In genetic research and medicine, polymerase chain reaction (PCR) is a commonly used technique for amplifying DNA samples for various experiments. This process is highly dependent on PCR consumables that are essential for a successful experiment. In this article, we discuss the essential consumables for a comprehensive PCR experiment: PCR plates, PCR tubes, sealing membranes, and pipette tips.
PCR plate:
PCR plates are one of the most important consumables in any PCR experiment. They are designed for rapid temperature cycling and provide uniform heat transfer within the bore for ease of handling. The plates are available in a variety of configurations including 96-well, 384-well, and 1536-well.
PCR plates are made of plastic, which makes them reliable and easy to handle. In addition, some PCR plates are specially coated to inhibit the binding of DNA molecules and prevent contamination. The use of PCR plates is critical to reducing labor-intensive steps previously performed in microcentrifuges or PCR machines.
PCR tube:
PCR tubes are small tubes, usually made of polypropylene, used to hold the PCR reaction mixture during amplification. They come in a variety of colors, but the most common are clear and translucent. Clear PCR tubes are often used when users want to view amplified DNA because they are transparent.
These tubes are designed to withstand the high temperatures and pressures found in PCR machines, making them ideal for PCR experiments. In addition to amplification, PCR tubes can be used for other applications such as DNA sequencing and purification and fragment analysis.
Sealing film:
Seal film is an adhesive plastic film attached to the top of a PCR plate or tube to prevent evaporation and contamination of the reaction mixture during PCR. Sealing films are extremely important in PCR experiments, as exposed reaction mixtures or any environmental contamination in the plate can compromise the validity and effectiveness of the experiment.
Made of polyethylene or polypropylene, depending on the application, these plastic films are highly heat resistant and autoclavable. Some films are pre-cut for specific PCR plates and tubes, while others come in rolls and can be used with a variety of PCR plates or tubes.
Pipette tips:
Pipette tips are essential consumables for PCR experiments, as they are used to transfer small amounts of liquid, such as samples or reagents. They are usually made of polyethylene and can hold liquid volumes from 0.1 µL to 10 mL. Pipette tips are disposable and intended for single use only.
There are two types of pipette tips – filtered and non-filtered. Filter tips are suitable to prevent any aerosol or droplet contamination from occurring, while non-filter tips are used for PCR experiments using inorganic solvents or caustic solutions.
In summary, PCR plates, PCR tubes, sealing membranes, and pipette tips are just some of the basic consumables needed for a comprehensive PCR experiment. By ensuring you have all the necessary consumables, you can better perform PCR experiments efficiently and with the accuracy you need. Therefore, always make sure you have enough of these consumables readily available for any PCR experiment.
At Suzhou Ace Biomedical, we are dedicated to providing you with the highest quality lab supplies for all your scientific needs. Our range of pipette tips, PCR plates, PCR tubes, and sealing film are meticulously designed and crafted to ensure precision and accuracy in all your experiments. Our pipette tips are compatible with all major brands of pipettes and come in a variety of sizes to fit your specific needs. Our PCR plates and tubes are made from high-quality materials and are designed to withstand multiple thermal cycles while maintaining sample integrity. Our sealing film provides a tight seal to prevent evaporation and contamination from outside elements. We understand the importance of reliable and efficient lab supplies, which is why we strive to provide you with the best products and services possible. Our team of experts is always available to assist you with any questions or concerns you may have.
Post time: May-08-2023