Tips, as consumables used with pipettes, generally can be divided into: ①. Filter tips , ②. Standard tips, ③. Low adsorption tips, ④. No heat source, etc.
1. The filter tip is a consumable designed to avoid cross-contamination. It is often used in experiments such as molecular biology, cytology, and virology.
2. Standard tip is the most widely used tip. Almost all pipetting operations can use ordinary tip, which is the most economical type of tip.
3. For experiments with high sensitivity requirements, or precious samples or reagents that are easy to remain, you can choose a low-adsorption tip to increase the recovery rate. The surface of the low-adsorption tip has undergone a hydrophobic treatment, which can reduce the low surface tension liquid leaving more residues in the tip. (The picture is not complete and the memory is limited)
PS: The wide-mouth tip is ideal for sucking viscous materials, genomic DNA, and cell culture fluid;
The performance indicators of the tip: low adsorption, filter element, tightness, force of loading and ejection, no DNase and RNase, no pyrogen;
How to choose a good tip? “As long as the tip that can be installed is the tip that can be used”
——This is the general understanding of almost all users on the adaptability of the suction head. This statement can be said to be partly true but not entirely true.
The tip that can be mounted on the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is this reliable? A question mark is required here. Answering this question requires data to speak.
1. You may wish to perform a performance test after matching the pipette with the tip. After rinsing the tip, perform several repeated sample addition operations, weigh the sample addition amount each time, and record the reading.
2. Calculate the accuracy and precision of the pipetting operation after converting it into volume according to the density of the test liquid.
3. What we have to choose is a tip with good accuracy. If the accuracy of the pipette and the tip is not good, it means that the tightness of the tip and the pipette cannot be guaranteed, so that the results of each operation cannot be reproduced.
So what are the minimum points for a good tip?
A good tip depends on concentricity, taper, and the most important point is adsorption;
1. Let’s talk about the taper first: if it is better, the match with the gun will be very good, and the liquid absorption will be more accurate;
2. Concentricity: The concentricity is whether the circle between the tip of the tip and the link between the tip and the pipette is the same center. If it is not the same center, it means that the concentricity is not good;
3. Finally, the most important one is our adsorptivity: the adsorptivity is related to the material of the tip. If the material of the tip is not good, it will affect the accuracy of the pipetting, resulting in a large amount of liquid retention or abbreviation To hang on the wall, causing errors in pipetting;
So everyone should pay special attention to the above three points when choosing a suction head
Post time: Oct-30-2021