I-Pipette ingelinye lamathuluzi avamile asetshenziswa kulabhorethri yebhayoloji, yomtholampilo, neyokuhlaziya lapho uketshezi ludinga ukukalwa ngokunembile futhi ludluliselwe lapho kwenziwa ukuhlanjululwa, ukuhlola noma ukuhlola igazi. Atholakala njenge:
① isiteshi esisodwa noma iziteshi eziningi
② ivolumu engashintshi noma elungisekayo
③ okwenziwa ngesandla noma ngogesi
Ayini ama-Pipettes e-Single-Channel?
I-pipette yesiteshi esisodwa ivumela abasebenzisi ukuthi badlulise i-aliquot eyodwa ngesikhathi. Lezi zivamise ukusetshenziswa kumalabhorethri anomthamo omncane wamasampula, okungaba yilawo abandakanyeka ocwaningweni nasekuthuthukisweni.
I-pipette yesiteshi esisodwa inekhanda elilodwa elifisa noma likhiphe amazinga anembe kakhulu oketshezi ngokusebenzisa okulahlwayo.ithiphu. Angasetshenziselwa izinhlelo zokusebenza eziningi ngaphakathi kwamalabhorethri anomsebenzi omncane kuphela. Lokhu kuvame ukuba amalabhorethri enza ucwaningo oluhlobene namakhemikhali okuhlaziya, isiko lamaseli, izakhi zofuzo noma i-immunology.
Ayini ama-Multi-Channel Pipettes?
Amapayipi eziteshi eziningi asebenza ngendlela efanayo namapayipi esiteshi esisodwa, kodwa asebenzisa amaningiamathiphuukulinganisa nokukhipha amanani alinganayo oketshezi ngesikhathi esisodwa. Ukusetha okujwayelekile kuyiziteshi eziyisi-8 noma eziyi-12 kodwa amasethi eziteshi angu-4, 6, 16 kanye nama-48 nawo ayatholakala. Izinguqulo ezingu-96 zebhentshitop yeziteshi nazo zingathengwa.
Usebenzisa i-pipette enamashaneli amaningi, kulula ukugcwalisa ngokushesha umthombo ongu-96-, 384-, noma ongu-1,536.ipuleti le-microtiter, okungase kuqukathe amasampula ezinhlelo zokusebenza ezifana nokukhulisa i-DNA, i-ELISA (ukuhlolwa kokuxilonga), izifundo ze-kinetic kanye nokuhlolwa kwamangqamuzana.
Ishaneli Eyodwa vs. Ama-Pipettes eziteshi eziningi
Ukusebenza kahle
I-pipette yesiteshi esisodwa ilungile uma wenza umsebenzi wokuhlola. Lokhu kungenxa yokuthi ikakhulukazi kuhilela ukusebenzisa amashubhu ngamanye, noma ukulinganisa okukodwa ukwenza ekumpontshelweni kwegazi.
Kodwa-ke, lokhu kuba yithuluzi elingasebenzi ngokushesha lapho kukhuphuka umthamo. Uma kunamasampuli/ama-reagents amaningi okufanele adluliselwe, noma ama-assay amakhulu asetshenziswayo96 amapuleti microtitre kahle, kunendlela ephumelela kakhulu yokudlulisa uketshezi bese usebenzisa i-pipette yomzila owodwa. Ngokusebenzisa i-pipette yamashaneli amaningi esikhundleni salokho, inani lezinyathelo zamapayipi liyancipha kakhulu.
Ithebula elingezansi libonisa inani lezinyathelo zamapayipi ezidingekayo zokusetha isiteshi esisodwa, isi-8 kanye nese-12.
Inombolo yezinyathelo zamapayipi ezidingekayo (6 ama-reagents x96 Well Microtitre Plate)
I-Pipette enesiteshi esisodwa: 576
I-8-Channel Pipette: 72
I-12-Channel Pipette: 48
Umthamo Wokushaya Amapayipi
Umehluko owodwa oyinhloko phakathi kwe-pipette eyodwa kanye namashaneli amaningi umthamo wequla ngalinye elingadluliswa ngesikhathi esisodwa. Nakuba kuncike kumodeli osetshenziswayo, ngokuvamile awukwazi ukudlulisa ivolumu eningi ngekhanda ngalinye ku-pipette eneziteshi eziningi.
Ivolumu ye-pipette yesiteshi esisodwa engadlulisa ibanga phakathi kuka-0.1ul no-10,000ul, lapho ububanzi be-pipette yeziteshi eziningi buphakathi kuka-0.2 no-1200ul.
Isampula Iyalayisha
Ngokomlando, ama-pipette aneziteshi eziningi abengalawuleki futhi kunzima ukuwasebenzisa. Lokhu kubangele ukulayishwa kwesampula okungahambisani, kanye nobunzima bokulayishaamathiphu. Kukhona amamodeli amasha atholakalayo manje, asebenziseka kalula futhi ahamba ngendlela ethile ukuze alungise lezi zinkinga. Kuyafaneleka futhi ukuqaphela ukuthi nakuba ukulayishwa koketshezi kungase kungalungile kancane nge-pipette eneziteshi eziningi, maningi amathuba okuba anembe kakhulu sekukonke kunesiteshi esisodwa ngenxa yokungalungi okwenzeka ngenxa yephutha lomsebenzisi ngenxa yokukhathala ( bheka isigaba esilandelayo).
Ukunciphisa Iphutha Lomuntu
Amathuba ephutha lomuntu ancipha kakhulu njengoba inani lezinyathelo zepayipi lincipha. Ukwehluka kokukhathala nesithukuthezi kuyasuswa, okuholela kudatha nemiphumela enokwethenjelwa futhi ekwazi ukukhiqizwa kabusha.
Ukulinganisa
Ukuqinisekisa ukunemba nokunemba kwamadivayisi okuphatha uketshezi, ukulinganisa okuvamile kuyadingeka. I-ISO8655 evamile ithi isiteshi ngasinye kufanele sihlolwe futhi sibikwe. Uma i-pipette inamashaneli amaningi, kuthatha isikhathi eside ukulinganisa okungadla isikhathi.
Ngokusho kwe-pipettecalibration.net ukulinganisa okujwayelekile okungu-2.2 ku-pipette yamashaneli ayi-12 kudinga imijikelezo yamapayipi engama-48 kanye nesisindo se-gravimetric (imiqulu emi-2 x ukuphindaphinda okungu-2 x amashaneli ayi-12). Ngokuya ngesivinini somqhubi, lokhu kungathatha amahora angu-1.5 nge-pipette ngayinye. Amalabhorethri ase-United Kingdom adinga ukulinganiswa kwe-UKAS azodinga ukwenza isamba sesisindo segravimetric esingu-360 (imiqulu emi-3 x ukuphindaphinda okungu-10 x amashaneli ayi-12). Ukwenza le nombolo yokuhlola mathupha akusebenzi futhi kungase kudlule ukonga isikhathi esifinyelelwe ngokusebenzisa i-pipette eneziteshi eziningi kwamanye amalebhu.
Kodwa-ke, ukuze unqobe lezi zinkinga izinsizakalo zokulinganisa i-pipette ziyatholakala ezinkampanini eziningana. Izibonelo zalokhu i-Gilson Labs, i-ThermoFisher kanye ne-Pipette Lab.
Lungisa
Akuyona into abaningi abacabanga ngayo lapho bethenga i-pipette entsha, kodwa ukuphindaphinda kwamanye ama-pipettes amaningi akulungiseki. Lokhu kusho ukuthi uma isiteshi esi-1 sonakalisiwe, kungase kudingeke ukuthi kushintshwe i-multifold. Kodwa-ke, abanye abakhiqizi bathengisa ukushintshwa kweziteshi ngazinye, ngakho-ke qiniseka ukuthi uhlola ukulungiswa nomkhiqizi lapho uthenga i-pipette yamashaneli amaningi.
Isifinyezo – Single vs Multi-Channel Pipettes
I-pipette eneziteshi eziningi iyithuluzi elibalulekile layo yonke ilabhorethri enanoma yini engaphezu kwamasampula amancane kakhulu. Cishe kuzo zonke izimo umthamo omkhulu woketshezi oludingekayo ukuze udluliselwe ungaphakathi komthamo walelo naleloithiphuku-pipette eneziteshi eziningi, futhi kunezithiyo ezimbalwa kakhulu ezihlobene nalokhu. Noma yikuphi ukwenyuka okuncane kobunzima ekusebenziseni i-pipette yamashaneli amaningi kudlulwa kakhulu ukuncipha kwenetha komthwalo wokusebenza, okunikwe amandla ngenani elinciphe kakhulu lezinyathelo zamapayipi. Konke lokhu kusho ukunethezeka komsebenzisi okuthuthukisiwe, kanye nephutha lomsebenzisi elinciphile.
Isikhathi sokuthumela: Dec-16-2022