I-PIPETTE ingenye yamathuluzi ajwayelekile asetshenziswa ezindaweni ezibonakalayo, emtholampilo, kanye nasezindlini zokuhlaziya lapho uketshezi ludinga ukulinganiswa ngokunembile futhi ludluliselwe lapho wenza ama-dilutions, acpas noma ukuhlolwa kwegazi. Ziyatholakala njengoba:
① Isiteshi esisodwa noma isiteshi esiningi
② ivolumu ehleliwe noma eguqukayo
③ Manual noma nge-elekthronikhi
Yini ama-pipete esitendana esisodwa?
I-pipette yesiteshi esisodwa ivumela abasebenzisi ukuthi badlulise i-aliquot eyodwa ngasikhathi. Lokhu kuvame ukusetshenziselwa ama-laboratories ngokugcwala okuphansi kwamasampula, okuvame ukuba yilabo ababambe iqhaza ocwaningweni nasekuthuthukisweni.
I-pipette yesiteshi esisodwa inekhanda elilodwa ku-afisa noma likhiphe amazinga anembe kakhulu owuketshezi ngokusebenzisa okuchithwayouthiphu. Zingasetshenziselwa izinhlelo zokusebenza eziningi ngaphakathi kwama-laboratoratories ane-frocking encane kuphela. Lokhu kuvame ukuba yi-laboratoratories eyenza ucwaningo oluhlobene namakhemikhali wokuhlaziya, isiko leseli, i-genetics noma i-immunology.
Yini ama-pipete ama-pipete ahlukahlukene ahlukahlukene?
Umsebenzi we-Multi-Channel Pipette ngendlela efanayo namapayipi wesiteshi esisodwa, kepha asebenzisa okuningiamathiphuukukala nokusabalalisa amanani alinganayo kawuketshezi ngasikhathi sinye. Ukusetha okujwayelekile kuneziteshi ezi-8 noma eziyi-12 kepha amasethi wesiteshi wesiteshi esingu-4, 6, 16 no-48 ayatholakala. Izinhlobo ezingama-96 ze-Benchtop nezinguqulo nazo zingathengwa.
Kusetshenziswa i-pipette yesiteshi esiningi, kulula ukugcwalisa ngokushesha i-96-, 384-, noma 1,536-kahleipuleti le-microtat, okungaqukatha amasampula wezinhlelo zokusebenza ezifana ne-DNA Amplification, e-ELISA (Ukuhlolwa Ukuxilonga), Izifundo zeKinetic kanye nokuhlolwa kwamangqamuzana.
Isiteshi esisodwa se-vs vs ama-pipette ama-multi-channel
Ikhono lokwenza
I-pipette yesiteshi esisodwa ilungile lapho wenza umsebenzi wokuhlola. Lokhu kungenxa yokuthi ikakhulukazi kuhilela nje ukusebenzisa amashubhu ngamanye, noma umdlalo owodwa we-cross ukuze wenze ukumpontshelwa igazi.
Kodwa-ke, lokhu kushesha kuba ithuluzi elingasebenzi kahle lapho kudlula kuncishisiwe. Lapho kunamasampula amaningi / ama-reagents okudlulisela, noma ama-ages akhudlwana aqhutshwa ngaphakathiAmapuleti we-microtitre kahle, kunendlela ephumelelayo yokudlulisa uketshezi bese usebenzisa i-pipette yesiteshi esisodwa. Ngokusebenzisa i-pipette yesiteshi esiningi esikhundleni salokho, inani lezinyathelo zasePipetting lincishiswa kakhulu.
Ithebula elingezansi libonisa inani lezinyathelo zePipetting ezidingekayo kwisiteshi esisodwa, 8 no-12 Settups.
Inani lezinyathelo zePipetting ezidingekayo (ama-reagents ayi-6 x96 Well microtitre Plate)
I-Pipette yesiteshi esisodwa: 576
I-8-Channel Pipette: 72
I-12-Channel Pipette: 48
Ivolumu yepayiphu
Umehluko owodwa osemqoka phakathi kwama-Pipette angashadile futhi anhlobonhlobo yivolumu ngalinye elingadluliselwa ngasikhathi sinye. Yize kuncike kwimodeli esetshenziswayo, ngokuvamile awukwazi ukudlulisa umthamo omkhulu ngekhanda ngalinye ku-pipette yeziteshi eziningi.
IVolumu I-Pipette yesiteshi esisodwa ingadlulisela amabanga phakathi kwe-0,1UL ne-10,000ul, lapho uhla lwe-pipette yeziteshi eziningi luphakathi kuka-0.2 no-1200ul.
Ukulayisha isampula
Ngokomlando, ama-Pipette ama-Multi-Channels abelokhu engasebenzi futhi kunzima ukuyisebenzisa. Lokhu kudale ukulayisha okwesampula okungahambelani, kanye nobunzima bokulayishaamathiphu. Kunamamodeli amasha atholakalayo manje, asebenziseka kalula futhi ahambe ngendlela yokulungisa lezi zinkinga. Kuhle nokuqaphela ukuthi yize ukulayishwa koketshezi kungaba yinto engalingani kangako nge-pipette yesiteshi esiningi, maningi amathuba okuba anembe kakhudlwana kunesiteshi esisodwa ngenxa yephutha lomsebenzisi ngenxa yokukhathala ( bheka isigaba esilandelayo).
Ukunciphisa iphutha lomuntu
Amathuba wephutha lomuntu ancishiswa kakhulu njengoba inani lezinyathelo ze-Pipetting liyancipha. Ukuhlukahluka kusuka ekukhathaleni nasekucindezelekeni kuyasuswa, okuholela ekutheni idatha nemiphumela ethembekile futhi ephindekanze.
Ukulinganisa
Ukuqinisekisa ukunemba nokunemba kwamadivayisi wokuphatha awuketshezi, kudingeka ukulinganiswa okujwayelekile. Isiteshi esijwayelekile se-ISO8655 sithi isiteshi ngasinye kufanele sihlolwe futhi sibikwe. Iziteshi eziningi I-Pipette inesikhathi eside, kuthatha isikhathi eside ukulinganisa okungadliwa isikhathi.
Ngokuya nge-Pipetttalibration.net Ukulinganisa okujwayelekile kwe-2.2 I-Pipette engu-12-Channel kudinga ama-Pipetting Cycles angama-48 kanye nama-gravimetric wesisindo (2 volumes x 2 ukuphindaphinda iziteshi ze-X 12). Ngokuya ngejubane le-opharetha, lokhu kungathatha amahora angaphezu kuka-1.5 nge-pipette ngayinye. Ama-laboratories e-United Kingdom adinga ukulungiswa kwe-UKAS kungadinga ukwenza ingqikithi yama-gravimetric angu-360 (amavolumu ama-x 10 amaphilisi x 12 iziteshi). Ukwenza le nombolo yezivivinyo ngesandla kuba nzima futhi kungahle kudlule ukonga isikhathi okutholwe ngokusebenzisa i-pipette yesiteshi esiningi kwamanye ama-lab.
Kodwa-ke, ukunqoba lezi zinsizakalo zokulinganisa izinkinga zePipette zitholakala ezinkampanini eziningana. Izibonelo zalezi ngama-Gilson Labs, Thermofofler kanye ne-pipette lab.
Lungisa
Akuyona into abaningi abacabanga ngayo lapho bethenga i-pipette entsha, kepha inqwaba yama-pipete athile aneziteshi eziningi awalungiswa. Lokhu kusho ukuthi uma isiteshi esingu-1 silimele, lonke i-manifold kungenzeka ukuthi lithathelwe indawo. Kodwa-ke, abanye abakhiqizi bathengisa ukushintshwa kweziteshi ngazinye, ngakho-ke qiniseka ukuthi ubheka ukulungiswa nomenzi lapho uthenga i-multi-change pipette.
Isifinyezo - Ama-Pipette E-Multi-Channel
I-Pipette yesiteshi esiningi iyithuluzi elibalulekile kuzo zonke izintobula ezinalutho olungaphezu kwamasampula amancane kakhulu. Cishe zonke izimo ivolumu ephezulu yoketshezi edingekayo ekudluliseleni ingaphakathi komthamo ngamunyeuthiphuKu-pipette yesiteshi esiningi, futhi kunezingqinamba ezimbalwa kakhulu ezihambisana nalokhu. Noma yikuphi ukwanda okuncane kokuxakaniseka ekusebenziseni i-pipette yesiteshi esinama-multi amaningi kudlule kakhulu ukwehla kwethayini ngokulayisha umsebenzi, kunikwe amandla inani elincishisiwe kakhulu lezinyathelo zePipetting. Konke lokhu kusho induduzo yomsebenzisi ethuthukisiwe, futhi yehlise iphutha lomsebenzisi.
Isikhathi sePosi: Dece-16-2022