Kungani Izinto Ezisetshenziswayo Zaselabhorethri Zidingeka Ukuze I-DNase kanye Ne-RNase Imahhala?

Emkhakheni webhayoloji yamangqamuzana, ukunemba nokwethembeka kubaluleke kakhulu. Noma yikuphi ukungcoliswa kwezinto ezisetshenziswayo kulabhorethri kungaholela emiphumeleni eyiphutha, okungaba nemiphumela emibi kakhulu ocwaningweni lwesayensi nokuxilongwa. Umthombo owodwa ovamile wokungcola ubukhona be-DNase kanye nama-enzyme e-RNase. Lawa ma-enzyme alulaza i-DNA ne-RNA, ngokulandelana, futhi angatholakala kumatrices ahlukahlukene webhayoloji. Ukuze kuncishiswe ingozi yokungcola futhi uqinisekise imiphumela enembile, izinto ezisetshenziswa elabhorethri, njengeamathiphu we-pipette, amapuleti omthombo ojulile, Amapuleti e-PCR, namashubhu, kufanele kube mahhala i-DNase ne-RNase.

Ama-enzyme e-DNase kanye ne-RNase atholakala yonke indawo futhi angatholakala emithonjeni ehlukahlukene yezinto eziphilayo, okuhlanganisa umzimba womuntu, izitshalo, nama-microorganisms. Badlala indima ebalulekile ezinqubweni zamaselula njengokuhlukana kwe-DNA, ukulungiswa kwe-DNA, nokuwohloka kwe-RNA. Nokho, ukuba khona kwazo endaweni yaselabhorethri kungaba yingozi ekuhloleni okubandakanya ukuhlaziywa kwe-DNA ne-RNA.

Amathiphu e-Pipette angenye yezinto ezisetshenziswa kakhulu elabhorethri. Asetshenziselwa ukuphatha uketshezi olunembile nokunembayo, okulwenza lubaluleke kakhulu ezinhlelweni ezihlukahlukene ezifana nokulungiselela isampula, ukulandelana kwe-DNA, kanye ne-PCR. Uma amathiphu e-pipette engeyona i-DNase ne-RNase mahhala, ukungcola kungenzeka ngesikhathi sokubhoboza, okuholela ekulimazeni amasampula e-DNA noma e-RNA. Lokhu kungase kubangele imiphumela engemihle noma engaphelele, efaka engozini ubuqotho baso sonke isilingo.

Amapuleti emithombo ejulile angenye ilabhorethri ebalulekile esetshenziswayo, ikakhulukazi ezinhlelweni ezisetshenziswa kakhulu. Zisetshenziselwa ukugcinwa kwesampula, ukuhlanjululwa kwe-serial, kanye nesiko lamaseli. Uma lezi zingcwecwe zingenayo i-DNase ne-RNase, noma imaphi amasampula e-DNA noma e-RNA agcinwe kuwo angangcoliswa, okuholela ekulimaleni kwama-nucleic acid. Lokhu kungonakalisa ukunemba kwezinhlelo zokusebenza ezingezansi ezifana ne-PCR, qPCR, noma ukulandelana kwesizukulwane esilandelayo.

Ngokufanayo, amapuleti e-PCR namashubhu ayizingxenye ezibalulekile ezinhlelweni ze-polymerase chain reaction (PCR). I-PCR iyindlela esetshenziswa kabanzi yokukhulisa ukulandelana kwe-DNA. Uma amapuleti e-PCR namashubhu kungcoliswe i-DNase noma i-RNase, inqubo yokukhulisa ingahle ibe sengozini, okuholela emiphumeleni engalungile kanye nezincazelo ezingamanga. I-DNase kanye ne-RNase-free PCR consumables ivimbela ukucekelwa phansi kwe-DNA eqondiwe noma i-RNA phakathi nenqubo yokukhulisa, kuqinisekisa imiphumela ethembekile nephinde ikhiqizeke.

Ukuze kubhekwane nenkinga yokungcola, izinto ezisetshenziswayo zaselabhorethri zidinga ukukhiqizwa ngezinqubo ezilawulwa kakhulu nezinto ezigunyazwe ukuthi azikho mahhala i-DNase ne-RNase. Izinkampani ezifana ne-Suzhou Ace Biomedical Technology Co., Ltd., zisebenza ngokukhethekile ekukhiqizeni izinto ezisetshenziswa elabhorethri ezihlangabezana nalezi zidingo eziqinile. Njengomkhiqizi ohamba phambili kulo mkhakha, i-Suzhou Ace Biomedical Technology Co., Ltd. ibeka phambili ikhwalithi nokwethembeka.

I-Suzhou Ace Biomedical Technology Co., Ltd. iyabuqonda ubunjalo obubucayi bokungcoliswa kwe-DNase ne-RNase ezintweni ezisetshenziswayo zaselabhorethri. Amathiphu abo e-pipette, amapuleti emithombo ejulile, amapuleti e-PCR, namashubhu konke kukhiqizwa kusetshenziswa izinto ezisezingeni eliphezulu ezithatha izinyathelo eziqinile zokulawula ikhwalithi ukuze kuqinisekiswe ukuthi azikho i-DNase ne-RNase.

Inkampani isebenzisa amasu okukhiqiza athuthukile futhi inamathela ezindinganisweni zekhwalithi eziqinile ukuze iqede ubungozi bokungcola, ngaleyo ndlela iqinisekise imiphumela enembile nethembekile kubacwaningi kanye nodokotela ngokufanayo. Bayaqonda ukuthi noma yikuphi ukuyekethisa kukhwalithi yezinto ezisetshenziswayo zaselabhorethri kungaba nemiphumela efinyelela kude, hhayi kuphela ocwaningweni kodwa nasezinhlelweni zomtholampilo lapho ukuxilonga okunembile kubalulekile.

Sengiphetha, izinto ezisetshenziswa elabhorethri ezifana namathiphu e-pipette, amapuleti emithombo ejulile, amapuleti e-PCR, namashubhu kufanele kube mahhala i-DNase ne-RNase ukuze kuqinisekiswe ukunemba nokuthembeka kokuhlolwa kwebhayoloji yamangqamuzana. Ukungcoliswa ngalawa ma-enzyme kungaholela ekulimazeni amasampula e-DNA kanye ne-RNA, kubeke engcupheni ukufaneleka kwemiphumela etholiwe. Izinkampani ezifanaI-Suzhou Ace Biomedical Technology Co., Ltd. ukuqonda ukubaluleka kokukhiqiza izinto ezisetshenziswayo ezihlangabezana nalezi zidingo eziqinile, okuvumela ososayensi nabelaphi ukuthi benze umsebenzi wabo ngokuzethemba nangokunemba.