Yimaphi amapuleti okufanele ngikhethe ukukhishwa kwe-nucleic acid?

Ukukhethwa kwamapuleti okukhishwa kwe-nucleic acid kuya ngendlela ethile yokukhishwa esetshenziswayo. Izindlela ezahlukahlukene zokukhishwa zidinga izinhlobo ezahlukene zamapuleti ukufezekisa imiphumela efanele. Nazi izinhlobo ezimbalwa ezisetshenziswa kakhulu zepuleti zokukhishwa kwe-nucleic acid:

1. 96-Well PCR PLUS: Lawa mapuleti asetshenziswa kakhulu ngezindlela zokukhishwa kwe-nucleic acid. Ziyahambisana nezinhlelo ezizenzakalelayo zokuphatha uketshezi futhi zingabamba amavolumu amancane wesampula.
2. Amapuleti ajulile ajulile: La mapuleti anesilinganiso esikhudlwana sevolumu kunamapuleti e-PCR futhi asetshenziselwa izindlela zokukhishwa kwencwajana noma ezenzakalelayo ze-nucleic acid ezidinga amanani amakhulu esampula.
3. Amakholamu e-spin: Lawa makholomu asetshenziselwa izindlela zokukhishwa kwe-nucleic acid adinga ukuhlanzwa nokuhlushwa kwama-nucleic acid. Amakholomu agcwele ulwelwesi olususelwa ku-silica olubopha ama-nucleic acid futhi lizihlukanise kwezinye izinto ezingcolisayo.
4. Ubuhlalu bamandla kazibuthe Ubuhlalu buhlanganiswe nento ebopha ama-nucleic acid futhi ingahlukaniswa kalula kwezinye izinto ezingcolisayo zisebenzisa uMagnet.

Kubalulekile ukubheka iphrothokholi ethile noma ikhithi esetshenziselwa ukukhishwa kwe-nucleic acid ukuthola uhlobo olufanele lwepuleti ngendlela.

Ukukhishwa kwethu kokudla kwe-nucleic acid kwenzelwe ukuhlinzeka ngokukhishwa okuthembekile futhi okusebenzayo kwe-DNA ne-RNA kusuka ezinhlotsheni ezahlukahlukene zesampula. Okuqukethwe kwethu kuyahambelana nezinhlobonhlobo zezindlela zokukhishwa kanye namapulatifomu, kufaka phakathi izindlela zezandla nezizenzakalelayo.

Umugqa wethu womkhiqizo ufakaAmapuleti we-PCR, Amapuleti ajulile ajulile, Amakholamu e-SPEP, kanye nobuhlalu bakaMagnetic, bonke benzelwe ukufeza izidingo zezinqubo ezihlukile zokukhishwa. Amapuleti ethu e-PCR namapuleti ajulile enziwe ngezinto ezisezingeni eliphakeme ukuze aqinisekise ukuhambisana nezinhlelo zokuphatha uketshezi ezizenzakalelayo kanye nokubekezelela amaphrothokholi akwakhiyelwa aqinile. Amakholamu ethu we-spin agcwele ulwelwesi olususelwa ku-silica olunikeza ukubopha okuhle kwama-nucleic acid kanye nokususwa okusebenzayo kokungcola. Ubuhlalu bethu bamazibuthe buhlanganiswa nento yokuphathelene enikezela amandla aphezulu okubopha kanye nokuhlukaniswa okusebenzayo kwama-nucleic acid avela kwezinye izinto zesampula.

Ukukhishwa kwethu kwezinto zokudla ze-nucleic acid zihlolwe kabanzi ngokusebenza nekhwalithi ukuqinisekisa imiphumela engaguquki. Sizibophezele ekuhlinzekeni amakhasimende ethu ngokudla okuphezulu kakhulu ukusekela izidingo zabo zokukhishwa kwe-nucleic acid.

Sicela uxhumane nathi ukuthola eminye imininingwane mayelana nokukhishwa kwethu kwezinto zokudla ze-nucleic acid nokuthi zingazuzisa kanjani ucwaningo lwakho noma izinhlelo zokusebenza zokuxilonga.