Imaphi amapuleti okufanele ngiwakhethele Ukukhishwa kwe-Nucleic Acid?

Ukukhethwa kwamapuleti okukhishwa kwe-nucleic acid kuncike endleleni ethile yokukhipha esetshenziswayo. Izindlela ezihlukene zokukhipha zidinga izinhlobo ezahlukene zamapuleti ukuze kuzuzwe imiphumela emihle. Nazi izinhlobo ezimbalwa zamapuleti ezivame ukusetshenziswa ekukhishweni kwe-nucleic acid:

  1. Amapuleti e-PCR angama-96: Lawa mapuleti avame ukusetshenziselwa izindlela zokukhipha i-nucleic acid ephezulu. Zihambisana nezinhlelo zokubamba uketshezi ezizenzakalelayo futhi zingabamba amasampula amancane.
  2. Amapuleti omthombo ojulile: Lawa mapuleti anomthamo wevolumu omkhulu kunamapuleti e-PCR futhi asetshenziselwa izindlela zokukhipha i-nucleic acid ezisebenza ngesandla noma ezizenzakalelayo ezidinga amavolumu amakhulu esampula.
  3. Spin amakholomu: Lawa makholomu asetshenziselwa izindlela zokukhipha i-nucleic acid ezidinga ukuhlanzwa nokugxiliswa kwama-nucleic acid. Amakholomu agcwele ulwelwesi olusekelwe ku-silica oluhlanganisa ama-nucleic acid futhi luwahlukanise kokunye ukungcola.
  4. Ubuhlalu kazibuthe: Ubuhlalu obuzibuthe buvame ukusetshenziselwa izindlela zokukhipha i-nucleic acid ezizenzakalelayo. Ubuhlalu bumbozwe ngento ebophezela kuma-nucleic acid futhi ingahlukaniswa kalula nokunye ukungcola kusetshenziswa uzibuthe.

Kubalulekile ukubonisana nephrothokholi ethile noma ikhithi esetshenziselwa ukukhipha i-nucleic acid ukuze kutholwe uhlobo lwepuleti olufanele lwale ndlela.

Ukukhishwa kwethu kwezinto ezisetshenziswayo ze-Nucleic Acid kuklanyelwe ukuhlinzeka ngokukhipha okuthembekile nokusebenza kahle kwe-DNA ne-RNA ezinhlotsheni zesampula ezihlukahlukene. Izinto zethu ezisebenzisekayo ziyahambisana nohlu lwezindlela zokukhipha kanye nezinkundla, okuhlanganisa izindlela ezenziwa ngesandla nezizenzekelayo.

Umugqa wethu womkhiqizo uhlanganisaAmapuleti e-PCR, amapuleti omthombo ojulile, amakholomu aphendukayo, nobuhlalu obuzibuthe, konke okudizayinelwe ukuhlangabezana nezidingo zamaphrothokholi ahlukene wokukhipha. Amapuleti ethu e-PCR kanye namapuleti emithombo ejulile enziwe ngezinto ezisezingeni eliphezulu ukuze kuqinisekiswe ukuhambisana nezinhlelo zokubamba uketshezi ezizenzakalelayo kanye nokumelana nemithetho eqinile yokukhipha. Amakholomu ethu ajikelezayo agcwele ulwelwesi olusekelwe ku-silica oluhlinzeka ngokubopha okuhle kakhulu kwama-nucleic acid kanye nokususwa okuphumelelayo kokungcola. Ubuhlalu bethu obukazibuthe bumbozwe ngento yokuphathelene enikeza amandla okubopha aphezulu kanye nokuhlukaniswa okusebenzayo kwama-nucleic acid kwezinye izingxenye zesampula.

Ukukhishwa kwethu kwezinto ezisetshenziswayo ze-Nucleic Acid kuhlolwe kakhulu ukusebenza nekhwalithi ukuze kuqinisekiswe imiphumela engaguquki. Sizibophezele ekunikezeni amakhasimende ethu izinto ezisetshenziswayo zekhwalithi ephezulu kakhulu ukuze zisekele izidingo zawo zokukhipha i-nucleic acid.

Sicela usithinte ukuze uthole ulwazi olwengeziwe mayelana ne-Extraction of Nucleic Acid Consumables yethu nokuthi ingaluzuzisa kanjani ucwaningo lwakho noma izinhlelo zokusebenza zokuxilonga.


Isikhathi sokuthumela: Feb-28-2023