kuyini ukuxilongwa kwe-in vitro?

I-in vitro diagnostics isho inqubo yokuhlonza isifo noma isimo ngokuhlukanisa amasampula ebhayoloji avela ngaphandle komzimba. Le nqubo incike kakhulu ezindleleni zebhayoloji yamangqamuzana ahlukahlukene, okuhlanganisa i-PCR kanye nokukhipha i-nucleic acid. Ukwengeza, ukuphatha uketshezi kuyisici esibalulekile sokuxilongwa kwe-in vitro.

I-PCR noma i-polymerase chain reaction iyindlela esetshenziselwa ukukhulisa izingcezu ezithile ze-DNA. Ngokusebenzisa iziqalo ezithile, i-PCR ivumela ukukhuliswa okukhethiwe kokulandelana kwe-DNA, okungase kuhlaziywe ngezimpawu zesifo noma ukutheleleka. I-PCR ijwayele ukusetshenziselwa ukuthola izifo ezibangelwa amagciwane, amabhaktheriya, fungal kanye nezimuncagazi, kanye nezifo zofuzo nomdlavuza.

Ukukhishwa kwe-Nucleic acid kuyindlela esetshenziselwa ukuhlukanisa nokuhlanza i-DNA noma i-RNA kumasampula ezinto eziphilayo. Ama-nucleic acid akhishiwe abe esetholakala ukuze ahlaziywe okwengeziwe, okuhlanganisa ne-PCR. Ukukhishwa kwe-Nucleic acid kubalulekile ekuhlonzweni okunembile kanye nokuhlelwa kokwelashwa kwezifo nezimo ezihlukahlukene.

Ukuphatha uketshezi kuyinqubo ebandakanya ukudluliswa okunembayo, ukukhipha kanye nokuxutshwa kwenani elincane loketshezi endaweni yaselabhorethri. Amasistimu okuphatha uketshezi okuzenzakalelayo aseye aduma kakhulu eminyakeni yamuva nje njengoba enika amandla ukudlula okuphezulu kanye nokunemba okukhulu ekuhlolweni okufana ne-PCR nokukhipha i-nucleic acid.

Ukuxilongwa kwe-in vitro kuncike kakhulu kulezi zindlela zebhayoloji yamangqamuzana ngoba zivumela ukutholwa nokuhlaziywa kwezimpawu zofuzo namangqamuzana ahlobene nesifo. Isibonelo, i-PCR ingasetshenziselwa ukukhulisa ukulandelana kwezakhi zofuzo ezithile ezihlobene nomdlavuza webele, kuyilapho ukukhishwa kwe-nucleic acid kungasetshenziswa ukuhlukanisa i-DNA etholakala ngesimila kumasampula egazi.

Ngokungeziwe kulawa masu, amanye amasu namadivayisi ahlukahlukene asetshenziswa ku-in vitro diagnostics. Isibonelo, amadivaysi e-microfluidic asetshenziswa kakhulu ezinhlelweni zokusebenza eziphezulu kanye nezindawo zokunakekelwa. Lawa madivayisi aklanyelwe ukuphatha ngokunembile futhi alawule amanani amancane oketshezi, okuwenza alungele i-PCR nezinye izinhlelo zokusebenza zebhayoloji yamangqamuzana.

Ngokunjalo, ubuchwepheshe bokulandelana kwesizukulwane esilandelayo (i-NGS) budlala indima ebaluleke kakhulu ekuxilongweni kwe-in vitro. I-NGS inika amandla ukulandelana okuhambisanayo kwezigidi zezingcezu ze-DNA, okuvumela ukutholwa okusheshayo nokunembile kokuguqulwa kofuzo okuhlobene nesifo. I-NGS inamandla okuguqula ukuhlonzwa nokwelashwa kwezifo zofuzo kanye nomdlavuza.

Kafushane, ukuhlolwa kwe-in vitro kuyingxenye ebalulekile yemithi yesimanje futhi kuncike kakhulu kumasu ebhayoloji yamangqamuzana afana ne-PCR, isizinda se-nucleic acid, nokuphatha uketshezi. Lobu buchwepheshe, kanye nobuchwepheshe obufana nemishini ye-microfluidic kanye ne-NGS, buyashintsha indlela esixilonga ngayo nokwelapha izifo. Njengoba ubuchwepheshe buqhubeka nokuthuthuka, ukuhlolwa kwe-in vitro cishe kuzonemba kakhudlwana futhi kuphumelele, ekugcineni kuthuthukise imiphumela yesiguli kanye nekhwalithi yokuphila.

At I-Suzhou Ace Biomedical,sizinikele ekukunikezeni izinsiza zelebhu ezisezingeni eliphezulu kuzo zonke izidingo zakho zesayensi. Uhlu lwethu lwamathiphu we-pipette, amapuleti e-PCR, amashubhu e-PCR, nefilimu yokuvala kuklanywe ngobunono futhi kwaklanywa ukuze kuqinisekiswe ukunemba nokunemba kukho konke ukuhlola kwakho. Amathiphu ethu e-pipette ahambisana nawo wonke ama-brand amakhulu ama-pipettes futhi eza ngobukhulu obuhlukahlukene ukuze uvumelane nezidingo zakho ezithile. Amapuleti ethu e-PCR namashubhu enziwe ngezinto zekhwalithi ephezulu futhi aklanyelwe ukumelana nemijikelezo eminingi yokushisa kuyilapho kugcinwa ubuqotho besampula. Ifilimu yethu yokuvala ihlinzeka ngokuvala okuqinile ukuvimbela ukuhwamuka nokungcoliswa yizinto zangaphandle. Siyakuqonda ukubaluleka kokunikezwa kwelebhu okuthembekile nokusebenzayo, yingakho silwela ukukunikeza ngemikhiqizo namasevisi angcono kakhulu ngangokunokwenzeka. Ithimba lethu lochwepheshe lihlala litholakala ukuze likusize nganoma imiphi imibuzo noma izinkathazo ongase ube nazo.