Iyini ipuleti le-PCR?

Ipuleti le-PCR liwuhlobo lwe-primer, i-dNTP, i-Taq DNA polymerase, i-Mg, i-nucleic acid yesifanekiso, i-buffer nezinye izithwali ezihilelekile ekuphenduleni kokukhulisa ku-Polymerase Chain Reaction (PCR).

1. Ukusetshenziswa kwepuleti le-PCR

Isetshenziswa kakhulu emkhakheni wezofuzo, i-biochemistry, i-immune, imithi, njll., hhayi kuphela ocwaningweni oluyisisekelo olufana ne-gene isolation, i-cloning kanye nokuhlaziywa kokulandelana kwe-nucleic acid, kodwa nasekuxilongweni kwezifo nanoma iyiphi indawo lapho kukhona i-DNA. kanye ne-RNA. Kuyinto esetshenziswa kanye kuphela elabhorethri. Umkhiqizo.

2.96 Yebo PCRIzinto zepuleti

Impahla yayo ikakhulukazi i-polypropylene (PP) namuhla, ukuze ikwazi ukuzivumelanisa kangcono nezilungiselelo zokushisa eziphindaphindwayo neziphansi kwinqubo yokusabela kwe-PCR, futhi ingafinyelela izinga lokushisa eliphezulu kanye nokuvala inzalo yokucindezela okukhulu. Ukuze kuzuzwe ukusebenza komphumela ophezulu ngokuhambisana nesibhamu esiwumugqa, umshini we-PCR, njll., amapuleti e-PCR angama-96 noma angu-384 asetshenziswa kakhulu. Umumo wepuleti uhambisana nendinganiso yamazwe ngamazwe e-SBS, futhi ukuze uvumelane nemishini ye-PCR yabakhiqizi abahlukene, ingahlukaniswa ngezindlela ezine zokuklama: akukho siketi, isiketi esiyingxenye, isiketi esiphakanyisiwe kanye nesiketi esigcwele ngokusho komklamo wesiketi.

3. Umbala oyinhloko wepuleti le-PCR

Ezivamile zisobala futhi zimhlophe, phakathi kwazo amapuleti e-PCR amhlophe afaneleka kakhulu ku-PCR entsha yesikhathi sangempela ye-fluorescent quantitative.