Yiziphi izinto ezisetshenziswayo ezidingekayo zokuhlolwa okuphelele kwe-PCR?

Ocwaningweni lwezakhi zofuzo nakwezokwelapha, i-polymerase chain reaction (PCR) iyindlela evame ukusetshenziswa yokukhulisa amasampula e-DNA okuhlola okuhlukahlukene. Le nqubo incike kakhulu ezintweni ezisebenzisekayo ze-PCR ezibalulekile ekuhloleni okuyimpumelelo. Kulesi sihloko, sidingida izinto ezisetshenziswayo ezibalulekile zokuhlolwa okuphelele kwe-PCR: amapuleti e-PCR, amashubhu e-PCR, ulwelwesi lokuvala, namathiphu e-pipette.

Ipuleti le-PCR:

Amapuleti e-PCR angenye yezinto ezisebenzisekayo ezibaluleke kakhulu kunoma yikuphi ukuhlola kwe-PCR. Zenzelwe ukuhamba ngebhayisikili okushesha kwezinga lokushisa futhi zinikeze ukudluliswa kokushisa okufanayo ngaphakathi kwebhore ukuze kube lula ukuphatha. Amapuleti atholakala ezinhlobonhlobo zokucushwa kufaka phakathi i-96-well, 384-well, kanye ne-1536-well.

Amapuleti e-PCR enziwe ngepulasitiki, okwenza athembeke futhi aphatheke kalula. Ngaphezu kwalokho, amanye amapuleti e-PCR aboshwe ngokukhethekile ukuze avimbele ukuboshwa kwama-molecule e-DNA futhi avimbele ukungcola. Ukusetshenziswa kwamapuleti e-PCR kubalulekile ekwehliseni izinyathelo ezidinga abasebenzi abaningi ezenziwa ngaphambilini kuma-microcentrifuges noma emishinini ye-PCR.

I-PCR tube:

Amashubhu e-PCR amashubhu amancane, ngokuvamile enziwe nge-polypropylene, asetshenziselwa ukubamba ingxube yokusabela kwe-PCR ngesikhathi sokukhulisa. Ziza ngemibala ehlukahlukene, kodwa ezivame kakhulu zicacile futhi ziyashintshashintsha. Amashubhu e-PCR acacile avame ukusetshenziswa lapho abasebenzisi befuna ukubuka i-DNA ekhulisiwe ngenxa yokuthi asobala.

Lawa mashubhu aklanyelwe ukumelana namazinga okushisa aphezulu nezingcindezi ezitholakala emishinini ye-PCR, okuwenza alungele ukuhlolwa kwe-PCR. Ngaphezu kokukhulisa, amashubhu e-PCR angasetshenziselwa ezinye izinhlelo zokusebenza ezifana nokulandelana kwe-DNA nokuhlanzwa kanye nokuhlaziywa kweziqephu.

Ifilimu yokuvala:

Ifilimu ye-Seal iyifilimu yepulasitiki enamathelayo enamathiselwe phezulu kwepuleti le-PCR noma ishubhu ukuvimbela ukuhwamuka nokungcoliswa kwengxube yokusabela ngesikhathi se-PCR. Amafilimu okubeka uphawu abaluleke kakhulu ekuhlolweni kwe-PCR, njengoba izingxube zokusabela eziveziwe noma noma yikuphi ukungcoliswa kwemvelo epuleti kungabeka engcupheni ukufaneleka nokusebenza kokuhlolwa.

Eyenziwe nge-polyethylene noma i-polypropylene, kuye ngokuthi isetshenziswa kanjani, lawa mafilimu epulasitiki ayakwazi ukumelana nokushisa futhi azikwazi ukuzenzela. Amanye amafilimu asikwa kusengaphambili amapuleti namashubhu e-PCR athile, kuyilapho amanye efika emiqulu futhi angasetshenziswa nezinhlobonhlobo zamapuleti noma amashubhu e-PCR.

Amathiphu we-Pipette:

Amathiphu e-Pipette ayizinto ezisetshenziswayo ezibalulekile zokuhlolwa kwe-PCR, njengoba asetshenziselwa ukudlulisa amanani amancane oketshezi, njengamasampuli noma ama-reagents. Ngokuvamile zenziwa nge-polyethylene futhi zingabamba amavolumu oketshezi ukusuka ku-0.1 µL kuya ku-10 mL. Amathiphu e-Pipette ayalahleka futhi ahloselwe ukusetshenziswa kanye kuphela.

Kunezinhlobo ezimbili zamathiphu e-pipette - ahlungiwe futhi angahlungiwe. Amathiphu okuhlunga afaneleka ukuvimbela noma yikuphi ukungcoliswa kwe-aerosol noma amaconsi ukuthi kungenzeki, kuyilapho amathiphu angahlungi asetshenziselwa ukuhlolwa kwe-PCR kusetshenziswa izinyibilikisi ze-inorganic noma izixazululo ze-caustic.

Kafushane, amapuleti e-PCR, amashubhu e-PCR, ulwelwesi lokuvala, namathiphu we-pipette angenye yezinto ezisetshenziswayo eziyisisekelo ezidingekayo ekuhloleni okuphelele kwe-PCR. Ngokuqinisekisa ukuthi unakho konke okusebenzisekayo okudingekile, ungenza kangcono izivivinyo ze-PCR ngokunemba okudingayo. Ngakho-ke, qiniseka ukuthi unokwanele kwalokhu okusebenzisekayo okutholakala kalula kunoma yikuphi ukuhlolwa kwe-PCR.

At I-Suzhou Ace Biomedical, sizinikele ekukunikezeni impahla yelebhu yekhwalithi ephezulu kakhulu yazo zonke izidingo zakho zesayensi. Ibanga lethu leamathiphu we-pipette, Amapuleti e-PCR, Amashubhu e-PCR, futhiifilimu yokuvalaaklanywe ngobunyoninco futhi aklanywa ukuze kuqinisekiswe ukunemba nokunemba kukho konke ukuhlola kwakho. Amathiphu ethu e-pipette ahambisana nawo wonke ama-brand amakhulu we-pipettes futhi afika ngobukhulu obuhlukahlukene ukuze uvumelane nezidingo zakho ezithile. Amapuleti ethu e-PCR namashubhu enziwe ngezinto zekhwalithi ephezulu futhi aklanyelwe ukumelana nemijikelezo eminingi yokushisa kuyilapho kugcinwa ubuqotho besampula. Ifilimu yethu yokuvala ihlinzeka ngokuvala okuqinile ukuvimbela ukuhwamuka nokungcoliswa yizinto zangaphandle. Siyakuqonda ukubaluleka kokunikezwa kwelebhu okuthembekile nokusebenzayo, yingakho silwela ukukunikeza ngemikhiqizo namasevisi angcono kakhulu ngangokunokwenzeka. Ithimba lethu lochwepheshe lihlala litholakala ukuze likusize nganoma imiphi imibuzo noma izinkathazo ongase ube nazo.