Uyayinqamulaithiphu ye-pipettelapho ufaka i-glycerol? Ngenza ngesikhathi sami se-PhD, kodwa kwadingeka ngifunde ukuthi lokhu kwandisa ukunemba nokungalungi kwepayipi lami. Futhi uma ngikhuluma iqiniso lapho ngisika ithiphu, ngangingase ngithelele ngokuqondile i-glycerol esuka ebhodleleni ngiyifaka eshubhuni. Ngakho-ke ngishintshe indlela yami yokuthuthukisa imiphumela ye-pipetting futhi ngithole imiphumela ethembekile futhi ekwazi ukukhiqiza kabusha lapho ngisebenza ngoketshezi lwe-viscous.
Isigaba se-liquid esidinga ukunakwa okukhethekile lapho i-pipetting inguketshezi olubonakalayo. Lezi zivame ukusetshenziswa elebhu, kungaba ngendlela ehlanzekile noma njengezingxenye zebhafa. Abameleli abadumile boketshezi lwe-viscous kuma-laboratories ocwaningo yi-glycerol, i-Triton X-100 ne-Tween® 20. Kodwa futhi, amalabhorethri enza ukulawula izinga lokudla, izimonyo, imithi kanye neminye imikhiqizo yabathengi ibhekana nezixazululo ze-viscous nsuku zonke.
I-viscosity ibizwa ngokuthi i-dynamic, noma i-kinematic viscosity. Kulesi sihloko ngigxila kakhulu ku-viscosity eguquguqukayo yoketshezi ngoba ichaza ukunyakaza koketshezi. Izinga le-viscosity licaciswe ku-millipascal ngomzuzwana (mPa*s). Kunalokho amasampula oketshezi angaba ngu-200 mPa*s afana no-85 % glycerol asengadluliswa kusetshenziswa i-piette ye-air-cushion yakudala. Uma usebenzisa inqubo ekhethekile, i-pipetting ehlehlayo, isifiso samabhamuza omoya noma izinsalela esiphundu ziyancipha kakhulu futhi kuholela emiphumeleni enembe kakhudlwana yamapayipi. Kodwa noma kunjalo, akuyona into engcono kakhulu esingayenza ukuze sithuthukise ipayipi ye-viscous liquids (bheka umdwebo 1).
Lapho i-viscosity ikhula, ubunzima buyanda. Izisombululo ze-viscous ezimaphakathi ezingafika ku-1,000 mPa*s zinzima kakhulu ukuthi zidluliselwe kusetshenziswa ama-pipette e-air-cushion yakudala. Ngenxa yokungqubuzana okuphezulu kwangaphakathi kwama-molecule, uketshezi lwe-viscous lunokugeleza okunensa kakhulu futhi ukufakwa kwamapayipi kufanele kwenziwe kancane kakhulu nangokucophelela. Inqubo yokuhlehlisa amapayipi ngokuvamile ayanele ukudluliswa koketshezi olunembile futhi abantu abaningi bakala amasampula abo. Leli su liphinde lisho ukucabangela ukuminyana koketshezi kanye nezimo zaselabhorethri ezinjengomswakama nezinga lokushisa ukuze kubalwe ngokunembile umthamo woketshezi oludingekayo ngesisindo. Ngakho-ke, kunconywa amanye amathuluzi e-pipetting, abizwa kanjalo ngamathuluzi okuhambisa kahle. Lezi zinethiphu enepiston ehlanganisiwe, njengesirinji. Ngakho-ke, uketshezi lungafiseleka kalula futhi lukhishwe kuyilapho kunikezwa ukudluliswa koketshezi okunembile. Indlela ekhethekile ayidingekile.
Noma kunjalo, amathuluzi amahle okufuduka afinyelela umkhawulo ngezixazululo ezibonakala kakhulu njengoju oluwuketshezi, ukhilimu wesikhumba noma amafutha athile ayimishini. Lolu ketshezi oludinga kakhulu ludinga elinye ithuluzi elikhethekile elisebenzisa isimiso esihle sokufuduka kodwa ngaphezu kwalokho linomklamo olungiselelwe ukubhekana nezixazululo ezibonakala kakhulu. Leli thuluzi elikhethekile liqhathaniswe namathiphu akhona wokufuduka ukuze uthole umkhawulo lapho kubalulekile ukushintsha usuka ephuzwini elivamile lokusabalalisa uye kwelinye ithiphu elikhethekile ukuze uthole izixazululo ezine-viscous. Kwaboniswa ukuthi ukunemba kuyanda futhi amandla adingekayo ekulangazeleleni nasekukhipheni ayancishiswa lapho kusetshenziswa ithiphu elikhethekile loketshezi olune-viscous. Ukuze uthole ulwazi oluthe xaxa nezibonelo eziwuketshezi, sicela ulande i-Applicaton Note 376 ekusebenzeni okuthuthukisiwe koketshezi olubonakalayo.
Isikhathi sokuthumela: Jan-23-2023