Izinto ezisetshenziswa ngama-reagent zingenye yezinto ezisetshenziswa kakhulu emakolishi nasezilabhorethri, futhi ziyizinto ezibalulekile kubahloli. Kodwa-ke, noma ngabe izinto ezisetshenziswayo ze-reagent ziyathengwa, zithengiwe noma ziyasetshenziswa, kuzoba nochungechunge lwezinkinga ngaphambi kokuphathwa kanye nabasebenzisi bezinto ezisetshenziswayo ze-reagent. Yiziphi izinkinga eziqondile? Ake ngikunikeze isingeniso esifushane.
Ukuze kuthengwe ama-reagents nezinto ezisetshenziswayo, ngenxa yolwazi lwazo lwe-asymmetry, oluhambisana neqiniso lokuthi umphakeli wama-reagents kanye nezinto ezisetshenziswayo uqashe abathengisi ukuze bathengiswe, amanani akhuphuka ngemuva kongqimba lokwenyuka kwentengo. Ngenxa yalokho, intengo yokuthenga i-reagent efanayo kumalabhorethri amabili eduze kwenyuvesi / ilabhorethri efanayo esitezi esisodwa ihluke kakhulu. Ukwengeza, iningi labasebenzi bocwaningo/abahlolayo bezesayensi abakwazanga ukuhlonza izimfanelo zomphakeli, okuholele ekutholeni [izimpahla zomgunyathi” kanye [nokuthengwa kwamanye amazwe okuhambisanayo”). Ekugcineni, basebenze kanzima isikhathi esingaphezu kwesigamu sonyaka sokuhlolwa, kodwa imiphumela yocwaningo yayiphelele ngoba bathenga ama-reagents mbumbulu. okungavumelekile. Ukwenziwa amanga kokusebenziseka kwe-reagent kuthinta kakhulu ucwaningo lwesayensi nemiphumela yokuhlolwa, futhi akuvamile ukuthi abacwaningi bachithe isikhathi esiningi, imali, namandla ocwaningweni olungaphumeleli. Ezinye izindlela zomgunyathi zifihliwe kakhulu. Le khithi ye-ELISA izophinda isebenzise umkhiqizo othile wenkomba ukuze ilingise amanye amakhithi ezinkomba. Kodwa uma kuqhathaniswa nendlela yangaphambilini ye-VEGF, okuyiphakheji yomkhiqizo, "i-smart" iwukuthi izinkomba ezisetshenziselwa ukushintshwa zihlukene kakhulu futhi zifihliwe, okunzima ukuzivimbela.
Ngakho-ke ngingawathola kanjani ama-reagents angempela nezinto ezisetshenziswayo ukuze ngigweme ukukhohliswa? Nazi izindlela ezimbalwa:
1. Thola izinto ezisetshenziswayo ezifanele nabahlinzeki be-reagent
Lapho uthenga izinto ezisebenzisekayo ze-reagent, kufanele ugweme ukuthenga izinto ezisebenzisekayo ze-reagent mbumbulu emthonjeni. Ngakho-ke, indlela yokukhetha umphakeli ofanele wama-reagents nezinto ezisetshenziswayo kubaluleke kakhulu. Ukukhethwa kwabahlinzeki-mpahla kungase kusekelwe emaphuzwini amabili: 1 ukukhetha izinhlobo ezingaphezu kwezimbili ezinkulu nabahlinzeki abanegama elihle; 2 ukusungula uhlelo lokulawula ukuhlinzekwa kwezidingo oluzwakalayo. Misa izindinganiso zokuhlola zabahlinzeki abaphindayo nabathengekayo, bhalisa ikhwalithi yokunikezwa ngakunye kwe-reagent kanye nezinto ezisebenzisekayo, futhi ube nendlela yokujezisa yokwephula, njengokubanqabela ukubamba iqhaza ekubhideni nasekuhlinzekeni ngesikhathi somjikelezo wokuhlinzeka. Ukukhetha abahlinzeki abangaphezu kwababili kungasetshenziswa ukuze kuqhathaniswe ikhwalithi kanye nentengo yezinhlangothi zombili, ukuze kuhlinzekwe ukukhetha okungcono nangaphezulu kwabasebenzi abahlobene emanyuvesi/amalebhu.
2. Funda amakhono alula okuhlonza
Kunezindlela eziningi zokuhlonza zama-reagents nezinto ezisetshenziswayo. Okulandelayo uhlu olufushane nje lwalokhu okubili:
1. Bheka iphakheji
Lapho sithola ama-reagents nezinto ezisetshenziswayo, kufanele siqale siqinisekise ukuthi uphawu aluzange ludatshulwe noma azikho ezinye izindlela zokuhamba. Uma uhlola ukuthi azikho yini izimpawu ezinyakazayo, naka ukuthi imigqa yephethini yophawu nemifanekiso ixhunywe ngendlela efanele yini. Uma imigqa yamaphethini nezithombe ingahambisani, ukupakishwa bekulokhu kwenziwa.
2. Bheka ilebula elimelene nokuguquguquka kombala/ukunamathela
Indlela enembile kakhulu yokuhlonza izinto ezisebenzisekayo ze-reagent ukushintsha i-engeli yokubuka, futhi ungabona ukuthi ilebula ephikisayo eshintsha umbala ephikisana nomgunyathi ivela kule mibala emibili elandelayo. Okokuqala, klwebha "ilebula yokulwa nomgunyathi" ephaketheni ukuze uthole ikhodi yokulwa nomgunyathi, bese ungena kuwebhusayithi esemthethweni ehambisanayo ukuze uhlole.
Isikhathi sokuthumela: Aug-09-2022