UNGAYEKA KANJANI UKUHLANGABEZA I-96 DEEP WELL PLATE

Ulahlekelwa ngamahora amangaki ngeviki ngenxa yamapuleti emithombo ejulile?

Umzabalazo ungokoqobo. Kungakhathaliseki ukuthi mangaki ama-pipette noma amapuleti owalayishile ocwaningweni lwakho noma emsebenzini, ingqondo yakho ingaqala ukudlala amaqhinga ngawe uma kukhulunywa ngokulayisha ipuleti lomthombo ojulile we-96.

Kulula kakhulu ukwengeza amavolumu kumthombo ongalungile noma umugqa ongalungile. Kulula ngephutha ukuphinda kabili ipuleti lomthombo ojulile ofanayo.

Noma ulayisha yonke isampula engalungile emithonjeni eminingi, okukubize amahora omsebenzi.

Noma, mhlawumbe wenze konke kahle, kodwa uqala ukuziqagela okwesibili. Iqala phansi.

Isikhathi sakho sibaluleke kakhulu. Ama-reagents akho abaluleke kakhulu. Futhi, okubaluleke kakhulu, idatha yakho ibaluleke kakhulu.

Akudingekile ukuthi sikutshele ukuthi yikuphi ukuchitha isikhathi lokhu, lapho ngokuvamile kufanele wenze kabusha ama-reagents futhi uxube. Futhi, akuzwakali kukuhle kangako nakuleveli yokuzethemba.

Nawa amathiphu namasu angcono kakhulu avela kwabanye ongaqala ukuwafaka esimisweni sakho ngelebhu.

Iyini ipuleti lomthombo ojulile elingu-96?

Isisekelo esivame ukunganakwa kumalebhu nasezikhungweni zokucwaninga yonke indawo, amapuleti emithombo ejulile alungele ukugcinwa kwamasampula esikhathi esifushane nesikhathi eside, ukulungiswa, nokuxuba. Angaba nomthombo oyisikwele noma phansi okuyindilinga.

Ukusetshenziswa kwabo kuyahlukahluka, kepha kuvame ukusetshenziswa ezinhlelweni zesayensi yempilo nasekusetshenzisweni kocwaningo, okubandakanya:

• Umsebenzi wamangqamuzana ezicubu nokuhlaziya amaseli
• Ukuhlolwa kwama-enzyme
• Izifundo ze-Proteomics
• Amadamu e-reagent
• Isitoreji sesampula esiphephile (kuhlanganise nesitoreji se-cryogenic)

Amathiphu aphezulu namasu okunqoba amaphutha angama-96 wepuleti elijulile

Sihlanganise uhlu lwamasistimu aphezulu nezindlela ezivela kozakwenu:

1. Hlola isimo sakho sengqondo futhi uhlale ugxilile:Njenganoma yini empilweni, amaphutha avamise ukwenzeka lapho ukhathele, ucindezelekile, noma uphazamisekile (... noma konke okungenhla). Yeka ukukhathazeka ngokusheshisa umsebenzi wakho. Yehlisa ijubane, futhi ucabange ngesinyathelo ngasinye ngokucophelela. Futhi uhlale ugxilile. Ukukhuluma nokusebenza kwenza eminye imisebenzi ihambe ngokushesha, kodwa hhayi ngalo msebenzi. Abanye abacwaningi balenga "Akukho ukukhuluma" bhalisa njengoba bephakathi nalo msebenzi. Umculo ophumuzayo (ikakhulukazi izinsimbi) uyakhuthazwa, nokho, uma udinga umsindo wangemuva ngenkathi usebenza!
2. Qondanisa amathiphu akho e-pipette nemithombo ehambisanayo:Ibhokisi elisha le-pipette lingcono kakhulu kumapuleti omthombo ojulile. Qondanisa umthombo nebhokisi njengoba uhamba. Yiba nebhokisi eliyisipele kokubekwe eceleni uma kwenzeka uphelelwa, ngakho-ke akumele uphazamise isistimu yakho uma udinga okwengeziwe. Sebenzisa amathiphu e-pipette ukuze ulandelele ukubala kahle.
3. Kubhale:Dala ishidi le-Excel lemiksi eyinhloko, kanye namamephu wepuleti ajulile angama-96. Umthombo ngamunye unegama leziqalo namasampula. Setha zonke izingxube zakho eziyinhloko ngendlela enengqondo, kanye nekhodi yombala yesethi ngayinye yokuqala (uma usebenzisa engaphezu kweyodwa). Hamba naleli shidi elebhu, bese ubheka umaka ishidi lapho uhamba. Ungase futhi ubhale amanani e-reagent kokuthunyelwe futhi ukugcine eduze kwakho njengokhiye wakho oyisampula njengoba ulayisha. Khetha isistimu ozosebenza ngayo (isb ngama-alfabhethi noma ngokwezinombolo, kuye ngokuthi abhalwa kanjani amakhodi) futhi ungalokothi uphambuke ohlelweni lwakho. Lapho wenza imiksi, beka yonke into ngononina ku-rack yakho, bese uyihambisa ekhoneni elikude uma usuqedile.
4. I-tape ingumngane wakho omusha omkhulu:Tapa ipuleti lonke, eceleni kwendawo oyilayishayo. Sebenza ngale ndlela ngale ndlela, uhambisa itheyiphu isikhathi ngasinye lapho isigaba siphelile. Ungalebula itheyipu yakho (isb. A – H, 1 – 12) ukukusiza ukuthi uhlale usendleleni.
   Isibonelo, ngenkathi ulayisha i-Gene A mastermix kumakholomu 1 kanye no-2 epuleti lakho lomthombo ojulile, qala ngokuthatha itheyipu bese umboza ngobumnene ikholomu 3 no-4. Ungenza ngisho le kholomu eyodwa ngesikhathi, ukuze uhlale uhlelekile. Kuyasiza ukuthi uhlale uqondile ngesikhathi semithombo ephakathi eqinile. Khumbula nje ukubamba ipuleti phansi kancane lapho ususa itheyiphu yakho, ukuze ugweme ukuchaphazeka.
5. Qhubeka nayo:Uma ubona ukuthi isistimu yakho ayisebenzi, ungayishintshi phakathi nendawo. Yishintshe ngaphambi noma ngemva kwalokho, kodwa ungalokothi uye phakathi (kuholela ekudidekeni okukhulu!).
6. Zijwayeze:Hlala uhambisana nenqubo oyikhethayo. Ukwenza lezi zinyathelo enkumbulo yemisipha kuzothatha isikhashana, kodwa ngokuhamba kwesikhathi kufanele uqale ukubona ukuthuthuka okuphawulekayo emsebenzini wakho (kanye nokukhungatheka okuncane kakhulu endaweni yakho yokusebenza!)

Khetha okokusebenza okufanele:

Kusuka ezintweni ezisetshenziswayo kuye kukhwalithi, imithombo eyindilinga noma iphansi lekholi, kunezinketho ezihlukahlukene lapho u-oda ipuleti elijulile le-96.

Okunye okucatshangelwayo kuhlanganisa:

• Material: Imaphi amasampuli owasebenzisayo? Ingabe umthombo wakho ojulile udinga ukumbozwa nge-lobind noma yenziwe nge-silicon?
• Usayizi: Ingakanani ivolumu edinga ukungena epuletini lakho elijulile elingu-96 PCR?
• Izinga lokushisa: Yimaphi amazinga okushisa imithombo yakho ejulile edinga ukumelana nayo?
• Yimaphi amandla e-centrifugation engakwazi ukumelana nepuleti yakho yomthombo ojulile engu-96?

Nakhu ososayensi abaningi abakusebenzisayo ezinhlelweni ezijwayelekile:

Lezi zingcwecwe ezilula ezingama-96 zeDeep Well

Lawa mapuleti emithombo ejulile asiza kanjani amalebhu nabaphathi balebhu:

• Anindlela elulaukuqoqa nokulungisa amasampula (njengoba kungekho ukushoda kwalezo zinto ezenzeka elebhu yakho nsuku zonke)
• Buyisa indawo yelebhu eyigugu, enekhono eliqinile lokupakisha elenza kube lula ukuzigcina kunangaphambili
• Gwema ukuchitheka ngeukuxuba okuthuthukisiwezamasampula akho amancane oketshezi
• Umklamo ukuthikunciphisa ukugcinwa ezindongeni, ngakho-ke umosha kancane isampula yakho
• Khokha33% ngaphansikunalokho obungakwenza kwamanye amabhrendi ahamba phambili

Izici zifaka:

• Iphansi eliyindilinga
• Iqhwa noma ifakwe efrijini (kufika ku -80 C)
• Ukuzinza - ngeke basabele ngama-solvents epuleti
• Ungafaki izinsimbi ezisindayo ukuze zithuthukiswe ngokuphephile
• Idizayinelwe ngokosayizi ojwayelekile wamazwe ngamazwe (i-SBS), izenze zifanelekele izindawo zokusebenza ezizenzakalelayo
• Vumela ukugcinwa koketshezi okuncane kwesampula yakho ezindongeni

Ukukhetha ipuleti elifanele lomthombo kungakusiza ukuthi ugweme:

• Amaphoyinti edatha aphuthelwe
• Ukuqalisa kabusha kwesampula
• Ukuhamba komsebenzi okunensayo
• Uphuthelwe umnqamulajuqu wephrojekthi

Jabulela ukucwaninga

Amapuleti emithombo ejulile angama-96 atholakala kumalebhu nasezikhungweni zocwaningo emhlabeni jikelele. Zingonga isikhathi, umzamo, nendawo yokugcina, kodwa isistimu efanele ibalulekile njengoba uqedela umsebenzi wakho.

Kusukela ekukhuleni komthamo wokugcina, kuya ekuxubeni okuthuthukisiwe, amapuleti emithombo ejulile alungele ikhemistri ehlanganisiwe nezicelo zelabhulali, amelana namakhemikhali amaningi, izincibilikisi, notshwala obusetshenziswa kukhemistri ehlanganisiwe.

Ilungele ukuqoqwa kwesampula, ukulungiswa kwesampula, nokugcinwa kwesampula yesikhathi eside (noma yesikhashana), amapuleti emithombo ejulile nomata bokuvala kungathuthukisa ukuhamba komsebenzi, futhi ipuleti elijulile elifanele lizokusiza ukuthi ukhiqize idatha yekhwalithi ephezulu kakhulu yezinhlelo zokusebenza ezivamile isayensi yempilo (nangaphezulu).