Ubani ongayazi i-acetone, i-ethanol & co. iqala ukuphuma ngaphandleithiphu ye-pipettengokuqondile ngemva kwesifiso? Mhlawumbe, sonke sike sabhekana nalokhu. Izindlela zokupheka eziyimfihlo njengokuthi “ukusebenza ngokushesha ngangokunokwenzeka” kuyilapho “ubeka amashubhu eduze kakhulu ukuze ugweme ukulahleka kwamakhemikhali nokuchitheka” kungokwemikhuba yakho yansuku zonke? Ngisho noma amaconsi amakhemikhali egijima ngokushesha kuyaye kubekezelelwe ukuthi ipayipi akusalungile. Izinguquko ezincane nje kumasu amapayipi, futhi ukukhetha okulungile kohlobo lwe-pipette kungasiza ukunqoba lezi zinselele zansuku zonke!
Kungani ama-pipettes edonsa?
Ama-pipette akudala aqala ukuconsa lapho efaka uketshezi olushintshashintshayo ngenxa yomoya ongaphakathi ku-pipette. Lokhu okubizwa ngokuthi i-air cushion kukhona phakathi kwesampula yoketshezi kanye nepiston ngaphakathi kwe-pipette. Njengoba kwaziwa ngokujwayelekile, umoya uyavumelana nezimo futhi uvumelana namathonya angaphandle afana nezinga lokushisa nomfutho womoya ngokukhula noma ukucindezelwa. Izinto eziwuketshezi nazo zingaphansi kwemithelela yangaphandle futhi ziyahwamuka ngokwemvelo njengoba umswakama womoya uphansi. Uketshezi oluguquguqukayo luhwamuka ngokushesha kakhulu kunamanzi. Ngesikhathi se-pipettting, iyahwamuka ingene emcamelweni womoya iphoqa owakamuva ukuthi ande futhi uketshezi lucindezelwe luphume ichopho le-pipette ... i-pipette iyaconsa.
Ungavimba kanjani uketshezi ukuthi lungaphumi
Enye indlela yokunciphisa noma yokumisa ukuconsa iwukuba ufinyelele izinga eliphezulu lomswakamo ku-cushion yomoya. Lokhu kwenziwa nge-pre-wetting theithiphu ye-pipettefuthi ngaleyo ndlela kugcwale umcamelo womoya. Uma usebenzisa uketshezi oluguquguqukayo oluphansi olufana no-70 % we-Ethanol noma u-1 % i-acetone, langazelela futhi ukhiphe uketshezi lwesampula ubuncane izikhathi ezi-3, ngaphambi kokulangazelela umthamo wesampula ofuna ukuwudlulisa. Uma ukugcwala koketshezi oluguquguqukayo kungaphezulu, phinda le mijikelezo yangaphambi kokumanzisa izikhathi ezingama-5-8. Kodwa-ke, ngokugxila okuphezulu kakhulu okufana no-100 % we-ethanol noma i-chloroform, lokhu ngeke kwanele. Kungcono ukusebenzisa olunye uhlobo lwe-pipette: i-piette displacement enhle. Lawa ma-pipette asebenzisa amathiphu anepiston ehlanganisiwe ngaphandle kwe-air cushion. Isampula lithintana ngqo nepiston futhi abukho ubungozi bokuconsa.
Iba umpetha wamapayipi
Ungathuthukisa kalula ukunemba kwakho lapho ufaka uketshezi olushintshashintshayo ngamapayipi ngokukhetha indlela efanele noma ngokushintsha ithuluzi olisebenzisayo. Ukwengeza, uzokwandisa ukuphepha ngokugwema ukuchitheka futhi wenze lula ukuhamba kwakho komsebenzi.
Isikhathi sokuthumela: Jan-17-2023