Ungavala kanjani i-PCR Plate

Isingeniso

Amapuleti e-PCR, okuyisisekelo selabhorethri iminyaka eminingi, ziya ziba ziningi nakakhulu esimweni sesimanje njengoba amalabhorethri enyusa umthamo wawo futhi aya ngokuya esebenzisa ama-automation phakathi nokusebenza kwawo. Ukufeza lezi zinhloso ngenkathi kugcinwa ukunemba nobuqotho bokuhlolwa kungaba nzima. Enye yezindawo ezivamile lapho amaphutha angangena khona iwukuvala uphawu lweAmapuleti e-PCR, ngendlela engalungile evumela ukuhwamuka kwamasampula, ukuguqula i-pH futhi ngenxa yalokho kuphazamise ukusebenza kwe-enzymatic, nokumema ukungcola. Ukufunda ukuvala aIpuleti le-PCRngendlela efanele iqeda lezi zingozi futhi iqinisekisa imiphumela ephindaphindekayo.

Thola Uphawu Olulungile Lwepuleti lakho le-PCR

I-Plate Caps vs. Film Seals vs. Lids
Ofelebaziyindlela enhle yokuvala ipuleti lakho ngophawu oluqinile, kuyilapho zisakunikeza amandla okuvula kalula futhi ulivale kabusha ipuleti njengoba ukudinga ngaphandle kokumosha. Kodwa-ke, ama-caps anezinkinga ezimbalwa ezibalulekile.

Okokuqala, kuzodingeka uthenge ikepisi ethile ehambisanayo, okuyenza ingaguquguquki. Kuzodingeka wenze isiqiniseko sokuthi ikepisi olikhethayo lilingana nepuleti, elincike kumkhiqizi walo, futhi ukhethe ukuthi idome noma iyisicaba ngokusekelwe kumshini wokushisa ukushisa oyisebenzisayo.

Okwesibili, ukusebenzisa ama-caps epuleti kungase kuphindaphindeke kakhulu futhi kuyisicefe, ngengozi yokungcola okuphambene uma ubeka i-cap engalungile emthonjeni ongalungile.

Yize ama-seal efilimu engaguquguquki kangako mayelana nokususa nokufaka esikhundleni, ayashintshashintsha kakhulu njengoba azolingana nanoma yiluphi uhlobo lwepuleti le-PCR, kungakhathalekile ukuthi ungubani umenzi. Zingamane zinqunywe ngobukhulu, zibenze ziphumelele kakhulu.

Enye inketho yisivalo sepuleti. Lokhu kunikeza ukuvikeleka okuncane kwamakepisi nama-seal, futhi asetshenziselwa kuphela ikhava yesikhashana ukuvimbela ukungcola.

I-Optical vs. I-Foil Film Seals

Kungakhathaliseki ukuthi udinga i-optical, uphawu olucacile noma uphawuifilimu ye-aluminium foilukuvala ipuleti lakho kunqunywa ngefomethi yakho yokuhlola.Amafilimu okuvalwa kwe-Opticalasobala ukuze akuvumele ukuthi ubheke amasampula, kuyilapho uwavikela futhi uvimbela ukuhwamuka. Ziwusizo futhi ikakhulukazi ekuhloleni kwe-qPCR okubandakanya ukwenza izilinganiso ezinembe kakhulu ze-fluorescence ngokuqondile kusuka epuletini, lapho uzodinga ifilimu elivalayo elihlunga njenge-fluorescence encane ngangokunokwenzeka. Kubalulekile ukwenza isiqiniseko sokuthi uphawu noma ikepisi oyisebenzisayo inezinga eliphezulu ngokwanele lokucaca kokubona ukuze uqinisekise ukuthi ukufundwa kunembile.

Amafilimu e-foil afanele noma imaphi amasampula azwela ukukhanya noma azogcinwa ngaphansi kuka-80°C. Ngenxa yalesi sizathu, amasampula amaningi amiselwe ukugcinwa isikhathi eside azodinga ifilimu ye-foil. Amafilimu e-foil nawo ayabhoboza, okuwusizo ekuhloleni imithombo ngayinye, noma ekudluliseni amasampula ngezinaliti. Lokhu kungenzeka mathupha noma njengengxenye yesikhulumi serobhothi.

Futhi cabanga ukuthi izinto ezinolaka ezihlanganisa ama-asidi, izisekelo noma izincibilikisi zizodinga uphawu olungamelana nazo, lapho i-foil seal ifaneleka kakhulu.

I-Adhesive vs. Heat Sealing Film
Ama-adhesive film sealsziqonde phambili futhi kulula ukuzisebenzisa. Okudingayo nje ukuthi umsebenzisi afake isigxivizo epuletini, futhi asebenzise ithuluzi lokufaka isicelo elilula ukuze acindezele phansi futhi akhe isigxivizo esiqinile.

Izivalo zokushisa zithuthuke kakhulu, zinikeza uphawu oluhlala isikhathi eside oluye lwanciphisa amazinga okuhwamuka uma kuqhathaniswa ne-adhesive seal evamile. Le nketho ifanelekile uma ubheke ukugcina amasampula esikhathini eside, nakuba lokhu kuza nemfuneko eyengeziwe yezinto zokusebenza zokuvala amapuleti.

Ungavala kanjani i-PCR Plate

I-Plate Sealing Method

Ukuzinamathisela

1. Qiniseka ukuthi usebenza endaweni yokusebenza eyisicaba futhi ezinzile

2. Susa ifilimu ephaketheni layo, bese ususa ukusekela

3. Faka uphawu ngokucophelela epuletini, ube uqinisekisa ukuthi imithombo ivalekile

4. Sebenzisa ithuluzi lokufaka isicelo ukufaka ingcindezi kuyo yonke ipuleti. Qala ukusuka komunye umkhawulo bese uhamba ngendlela yakho uye komunye, ucindezela ngokulinganayo

5. Phinda lokhu izikhathi eziningi

6. Gijimisa isifakeli sakho emithonjeni engaphandle, ukuze uqiniseke ukuthi nayo ivalwe kahle.

Ama-Heat Seals

Izimpawu zokushisa zisebenza ngokuncibilikisa ifilimu emgqeni womthombo ngamunye, ngosizo lwe-plate sealer. Ukuze usebenzise isiciko sokushisa, bheka imiyalelo enikezwe umenzi wezinto zokusebenza. Qiniseka ukuthi umkhiqizi othola kuye okokusebenza uthembekile, njengoba kubaluleke kakhulu ukuthi uphawu lufanele, lusebenze kahle futhi alungeni amanzi.

Amathiphu Aphezulu Okuvalwa Kweplate

a. Lapho ucindezela uphawu, hamba ngazo zombili izikhombisi-ndlela ezivundlile neziqondile ukuze uqinisekise uphawu olufanele.

b. Kuhlale kuwumkhuba omuhle ukuhlola ukuhlola kwanoma yini oyenzayo, futhi lokhu akufani nokuvala amapuleti. Hlola ngepuleti elingenalutho ngaphambi kokusebenzisa elinamasampuli.

c. Lapho uhlola, susa uphawu bese ubheka ukuze ubone ukuthi ingcina ibambelele kahle, ingenazikhala. Kukhona ukumelwa okubonakalayo kwalokhu emqulwini wokuqala oyinkomba. Uma ungazange uvale ipuleti ngendlela efanele, lapho ususa uphawu kuyoba nezikhala lapho okunamathelayo kungazange kwenziwe isibopho ngokugcwele epuleti.

d. Ukuze uthunyelwe nokuthutha amasampula, ungase ukuthole kuwusizo ukufaka uphawu lwepulasitiki phezu kwe-foil seal ukuze uthole ukuvikelwa okwengeziwe (ikakhulukazi ekubhobozeni).

e. Njalo qinisekisa ukuthi awekho amaqhubu noma imibimbi lapho ugcoba ifilimu - lokhu kuzodala ukuvuza nokuhwamuka.