Ukunemba nokunemba ngisho ne-pipette engcono kakhulu elinganisiwe kungasulwa uma ukhetha uhlobo olungalungile lwamathiphu. Kuye ngokuhlolwa okwenzayo, uhlobo olungalungile lwamathiphu lungenza futhi i-pipette yakho ibe umthombo wokungcola, iholele ekumoshweni kwamasampula ayigugu noma ama-reagents-noma ize ikubangele ukulimala ngokomzimba ngendlela yokulimala okuphindaphindiwe kokucindezeleka (RSI). Kunezinhlobo eziningi ezahlukene zamathiphu ongakhetha kuzo. Wazi kanjani ukuthi yikuphi okungcono kakhulu kwe-pipette yakho nesimo? Ungesabi, yilokho esikuzele lapha.
- 1) Khetha amathiphu we-pipette wekhwalithi ephezulu ngokunemba nokunemba
- 2) Amathiphu aqondile we-Universal noma we-Pipette?
- 3) Amathiphu we-Filter & Non-filter pipette. Izinzuzo kanye Nokuphazamiseka
- 4) Amathiphu okugcina aphansi
- 5) Amathiphu Ergonomic
1) Khetha amathiphu aphezulu we-pipette ukuze uthole ukunemba nokunemba
Ukucatshangelwa kokuqala okufika engqondweni uma ucabanga ukuthi yiluphi uhlobo lwethiphu ongalukhetha ukunemba nokunemba. Uma kukhona noma iyiphi i-batch-to-batch, noma ngaphakathi kwe-batch, ukuhluka kokuma kwamathiphu we-pipette, khona-keipayipi lakho ngeke linembe. Ukunemba kwe-pipette yakho kungathintekauma ithiphu ayilingani kahle i-pipette yakho. Uma kukhona uphawu olungalungile phakathi komgqomo wakho we-pipette kanye nethiphu, khona-ke umoya odonswe ngaphakathi ungaphuma futhi umthamo ofanele woketshezi awufiswanga. Ngakho-ke, umthamo wokugcina okhishiwe awulungile neze. Ukukhetha ithiphu elifanele i-pipette yakho kungaba ibhizinisi elikhohlisayo.
Okusiletha embuzweni….
2) Amathiphu aqondene nendawo yonke noma aqondene ne-Pipette?
Inketho engcono kakhulu ye-pipette yakho kanye nesicelo ukusebenzisa amathiphu aphezulu wendawo yonke. Lezi zeluleko zomhlaba wonke zingasetshenziswa nama-micropipettes amaningi emakethe. Amathiphu omhlaba wonke aklanyelwe ukulingana ngokuphephile futhi ngokuqinile ezungeze yonke imiphongolo ye-pipette, ehluka kancane ububanzi ukusuka kumkhiqizi kuya kumkhiqizi. Isibonelo, amathiphu anobuchwepheshe be-FlexFit ayavumelana nezimo ekugcineni kwephuzu (okungukuthi, eduze kakhulu nomgqomo), okubenza bavumelane kangcono nohlu olubanzi lwezinhlobo ze-pipette. Kuma-Labclinics, ungathola amathiphu wendawo yonke anazo zonke izici ezixoxwe ngezansi (isithiyo se-aerosol, iziqu, i-ergonomic, njll.).
3) Amathiphu Okuhlunga & Okungahlungi. Izinzuzo kanye nokuphazamiseka
Amathiphu okuvimbela, noma amathiphu okuhlunga, enzelwe izimo ezihlukene. Uma uzokwenza ipayipi into ongayenzangcolisa i-pipette yakho-ngokwesibonelo amakhemikhali aguquguqukayo, abolayo, noma abonakalayo - khona-ke uzofuna ukucabangela amathiphu okuvimbela ukuvikela i-pipette yakho namasampuli akho.
Amathiphu okuhlunga avimbela ukungcoliswa kwe-PCR
Amathiphu e-Aerosol Barrier, ebizwa nangokuthiamathiphu e-pipette yokuhlunga, afakwe isihlungi ngaphakathi kwengxenye eseduze yethiphu. Isihlungi sivikela ama-pipette akho kuma-aerosols kanye nezixazululo ezifisayo eziguquguqukayo noma ze-viscous emgqonyeni, konke okungangcolisa futhi kulimaze i-pipette. Lawa macebiso ngokuvamile afika enziwe inzalo ngaphambili kanye ne-DNase/RNase-free. Kodwa-ke, "umgoqo" uyigama elingalungile kwamanye alawa macebiso. Amathiphu athile kuphela aphezulu anikeza umgoqo wokuvala wangempela. Izihlungi eziningi zinciphisa uketshezi ekungeneni emgqonyeni we-pipette. Isithiyo sokuhlunga kulawa mathiphu siwenza abe yinketho yezinhlelo zokusebenza ezibucayi, njenge-qPCR. Umgoqo uvimbela ukungcoliswa kwe-PCR ngokumisa isampula ye-carryover kusuka ku-pipette, okuzokunikeza imiphumela enamandla kakhulu. Futhi, khumbula ukusebenzisa ukulawula kwakho okuhle kwe-PCR nokulawula okungekuhle ukuze uthole isampula ye-carryover. Ukwengeza, amathiphu okuhlunga 'amasondo okuqeqesha' amahle kwabasanda kuzalwa. Izikhathi eziningi ukungcoliswa kwe-pipette kwenzeka lapho ilungu lelebhu elisha lifisa ngephutha uketshezi ku-pipette ngokwayo. Kulula kakhulu, futhi kubiza ngempumelelo, ukulahla ithiphu kunokuthumela yonke ipayipi ukuze ilungiswe ngoba uketshezi lusepiston.
4) Amathiphu okugcina aphansi
Noma ngabe ukhetha liphi ithiphu, ukugcinwa okuphansi kuyisici esibalulekile. Amathiphu okugcina okuphansi enza njengoba nje negama liphakamisa—gcina amazinga aphansi oketshezi. Uma uke wabheka ithiphu yepayipi evamile, ungase ubone uketshezi oluncane olusele ngemva kokulukhipha. Amathiphu okugcina kancane anciphisa lokhu ukuthi kungenzeki ngoba ane-hydrophobic plastic additive egcina uketshezi lunganamatheli ngaphakathi kwamathiphu.
5) Amathiphu Ergonomic
Ukwenza imisebenzi ephindaphindayo, njengepayipi, kungabangela ukulimala kwamalunga futhi kubangele ukulimala okuphindaphindiwe kokucindezeleka (RSI). Ngenxa yalokhu, izinkampani zenze amathiphu e-ergonomic adinga amandla aphansi okufaka nokukhipha futhi, ngakho-ke, anciphisa ingozi ye-RSI. Lokho kusho, lesi sici sonke sibuyela ekulinganeni okuhle. Ithiphu eklanyelwe ngokuqondile ukulingana ne-pipette yakho ngokuchazwa ithiphu le-ergonomic.
Isikhathi sokuthumela: May-10-2022