Yini ama-Cryovials?
Izitsha zokugcina ze-Cryogenickukhona iziqukathi ezincane, ezinezingqimba neziyisilinda eziklanyelwe ukugcina nokugcina amasampula emazingeni okushisa aphansi kakhulu. Nakuba ngokwesiko lezi zitsha zenziwe ngengilazi, manje sezivame ukwenziwa nge-polypropylene ukuze kube lula nezizathu zezindleko. Ama-Cryovials aklanywe ngokucophelela ukuthi amelane namazinga okushisa afinyelela ku -196℃, futhi amukele inhlobonhlobo yamaseli. Lokhu kuyahlukahluka kusuka kuma-stem cells okuxilongwa, ama-microorganisms, amangqamuzana ayinhloko kuya kumaseli amisiwe. Ngaphandle kwalokho, kungase futhi kube nezidalwa ezincane ze-multicellular ezigcinwe ngaphakathiizitsha zokugcina ze-cryogenic, kanye ne-nucleic acid namaprotheni okudingeka agcinwe emazingeni okushisa okugcina i-cryogenic.
Izitsha zokugcina ze-Cryogenic ziza ngezindlela ezihlukahlukene, futhi ukuthola uhlobo olufanele olufeza zonke izidingo zakho kuzoqinisekisa ukuthi ugcina ubuqotho besampula ngaphandle kokukhokha ngokweqile. Funda ku-athikili yethu ukuze ufunde kabanzi mayelana nokucatshangelwa okubalulekile kokuthenga lapho ukhetha i-cryovial efanele yesicelo sakho selabhorethri.
Izakhiwo ze-Cryogenic Vial okufanele zicatshangelwe
Imicu Yangaphandle vs Yangaphakathi
Abantu bavame ukwenza lokhu kukhetha ngokusekelwe kulokho abakuthandayo, kodwa empeleni kukhona umehluko obalulekile wokusebenza okufanele ucatshangelwe phakathi kwezinhlobo ezimbili zentambo.
Amalabhorethri amaningi avame ukukhetha amabhodlela anentambo yangaphakathi ukuze anciphise indawo yokugcina amashubhu ukuze avumele ukungena kangcono emabhokisini efriji. Ngaphandle kwalokhu, ungase ucabange ukuthi inketho yentambo yangaphandle iyindlela engcono kakhulu kuwe. Zibhekwa njengezithwala ingozi ephansi yokungcola, ngenxa yomklamo okwenza kube nzima nakakhulu kunoma yini enye ngaphandle kwesampula ukungena ku-vial.
Izitsha ezifakwe ngaphandle ngokuvamile zikhethwa ezinhlelweni ze-genomic, kodwa noma iyiphi inketho ibhekwa njengefanele i-biobanking nezinye izinhlelo zokusebenza eziphezulu.
Into yokugcina okufanele uyicabangele ekufakeni intambo - uma ilabhorethri yakho isebenzisa i-automation, kungase kudingeke ucabangele ukuthi iyiphi intambo engasetshenziswa nezinsimbi zokubamba.
Ivolumu Yesitoreji
Izitsha ze-Cryogenic zitholakala ngobukhulu obuhlukahlukene ukumboza izidingo eziningi, kodwa ikakhulukazi zihluka phakathi komthamo we-1 mL no-5 mL.
Isihluthulelo siwukuqinisekisa ukuthi i-cryovial yakho ayigcwaliswanga ngokweqile nokuthi kunegumbi elengeziwe elitholakalayo, uma kwenzeka isampula livuvukala ngenkathi iqhwa. Empeleni, lokhu kusho ukuthi amalabhorethri akhetha ibhodlela elingu-1 mL lapho egcina amasampuli ka-0.5 mL wamaseli amiswe ku-cryoprotectant, kanye nezitsha ezingu-2.0 mL ngo-1.0 mL wesampula. Elinye icebiso lokungagcwalisi kakhulu izitsha zakho ukwenza ukuthi usebenzise ama-cryovials anezimpawu ezineziqu, okuzoqinisekisa ukuthi uvikela noma yikuphi ukuvuvukala okungadala ukuqhekeka noma ukuvuza.
I-Screw Cap vs Flip Top
Uhlobo oluphezulu olukhethayo luncike kakhulu ekutheni uzosebenzisa i-nitrogen yesigaba se-liquid noma cha. Uma kunjalo, uzodinga ama-cryovial ane-screw capped. Lokhu kuqinisekisa ukuthi azikwazi ukuvuleka ngephutha ngenxa yokungaphathwa kahle noma ukushintsha kwezinga lokushisa. Ukwengeza, ama-screw caps avumela ukubuyisa kalula kusuka kumabhokisi e-cryogenic kanye nesitoreji esisebenza kahle.
Kodwa-ke, uma ungasebenzisi i-nitrogen yesiteji ewuketshezi futhi udinga itop elula okulula ukuyivula, khona-ke i-flip top iyindlela engcono kakhulu. Lokhu kuzokongela isikhathi esiningi njengoba kulula kakhulu ukuyivula, okwenza kube wusizo ikakhulukazi ekusebenzeni okuphezulu kokusebenza kanye nalabo abasebenzisa izinqubo ze-batch.
Seal Security
Indlela engcono kakhulu yokuqinisekisa isivalo esivikelekile ukuqinisekisa ukuthi ikepisi lakho le-cryovial nebhodlela zombili zakhiwe ngento efanayo. Lokhu kuzoqinisekisa ukuthi ziyashwabana futhi zande ngazwi linye. Uma zenziwa kusetshenziswa izinto ezihlukene, zizoshwabana futhi zande ngamazinga ahlukene njengoba izinga lokushisa lishintsha, izikhala eziholayo kanye nokuvuza okungaba khona kanye nokungcola okuwumphumela.
Ezinye izinkampani zinikeza amawasha akabili kanye nama-flange ngezinga eliphezulu kakhulu lokuphepha kwesampula kuma-cryovial anentambo yangaphandle. Ama-cryovial e-O-Ring athathwa njengathembekile kakhulu kuma-cryovial anentambo yangaphakathi.
Ingilazi vs Ipulasitiki
Ukuze kuphephe futhi kube lula, ama-laboratories amaningi manje asebenzisa ipulasitiki, ngokuvamile i-polypropylene, esikhundleni sama-ampule engilazi avaliwe. Ama-ampole engilazi manje asebhekwa njengenketho ephelelwe yisikhathi njengoba phakathi nenqubo yokuvala ukuvuza kwemigodi engabonakali kungase kuvele, okuthi uma incibilika ngemva kokugcinwa ku-nitrogen ewuketshezi kubangele ukuthi aqhume. Futhi awafaneleki ngendlela yesimanje amasu okulebula, okuwukhiye wokuqinisekisa ukulandeleka kwesampula.
I-Self Standing vs Izansi Eziyindilinga
Izitsha ze-Cryogenic ziyatholakala zombili njengezizimele nezingezansi ezimise okwenkanyezi, noma njengezingezansi eziyindilinga. Uma udinga ukubeka izitsha zakho phezulu qiniseka ukuthi ukhetha ukuzimelela
Ukulandelelwa kanye Nokulandelela Isampula
Le ndawo yokugcina i-cryogenic ngokuvamile ayinakwa kodwa ukulandelela isampula nokulandeleka kuyisici esibalulekile okufanele sicatshangelwe. Amasampula e-Cryogenic angagcinwa iminyaka eminingi, okuthi ngokuhamba kwesikhathi abasebenzi bangashintsha futhi ngaphandle kwamarekhodi agcinwe kahle bangakwazi ukuhlonza.
Qiniseka ukuthi ukhetha izitsha ezenza ukuhlonza isampula kube lula ngangokunokwenzeka. Izinto okufanele uzibheke zihlanganisa:
Izindawo zokubhala ezinkulu zokurekhoda imininingwane eyanele ukuze kutholakale amarekhodi uma ibhodlela lisendaweni engalungile - ngokuvamile ubunikazi beseli, usuku oluqandisiwe, kanye nama-initials omuntu obhekene necala anele.
Amabhakhodi ukusiza ukuphathwa kwesampula kanye nezinhlelo zokulandela umkhondo
Amakepisi anemibala
Inothi lesikhathi esizayo - ama-chips amelana kakhulu nokubanda ayathuthukiswa okuthi, uma efakwe ngaphakathi kwe-cryovial ngayinye, akwazi ukugcina umlando wokushisa onemininingwane kanye nolwazi lwenqwaba enemininingwane, imiphumela yokuhlolwa kanye neminye imibhalo yekhwalithi efanele.
Ngaphezu kokucabangela ukucaciswa okuhlukene kwezitsha ezitholakalayo, omunye umcabango udinga futhi unikezwe inqubo yobuchwepheshe yokugcina ama-cryovial ku-nitrogen ewuketshezi.
Izinga Lokushisa Lesitoreji
Kunezindlela eziningana zokugcina isitoreji se-cryogenic samasampuli, ngayinye isebenza ezingeni lokushisa elithile. Izinketho nezinga lokushisa ezisebenza kulo zihlanganisa:
Isigaba soketshezi LN2: gcina izinga lokushisa elingu -196℃
Isigaba se-Vapor LN2: siyakwazi ukusebenza kumazinga okushisa athile aphakathi kuka -135°C no -190°C kuye ngemodeli.
Iziqandisi zomhwamuko we-nitrogen: -20°C kuya ku-150°C
Uhlobo lwamaseli agcinwayo kanye nendlela yokugcina ekhethwa umcwaningi izonquma ukuthi iyiphi inketho kwezintathu ezitholakalayo elabhorethri yakho ezisebenzisayo.
Nokho, ngenxa yamazinga okushisa aphansi kakhulu asetshenziswayo akuwona wonke amashubhu noma imiklamo ezofaneleka noma ephephe. Izinto ezisetshenziswayo zingashwabana ngokwedlulele emazingeni okushisa aphansi kakhulu, ukusebenzisa isitsha esingafanele ukusetshenziswa ezingeni lokushisa olikhethile kungabangela umkhumbi ukuthi uphahlazeke noma uqhekeke ngesikhathi sokugcinwa noma ukuncibilika.
Hlola ngokucophelela izincomo zabakhiqizi ngokusetshenziswa okufanele njengoba ezinye izitsha ze-cryogenic zifanele amazinga okushisa afinyelela ku- -175°C, amanye -150°C amanye nje ngo-80°C.
Kuyafaneleka futhi ukuqaphela ukuthi abakhiqizi abaningi bathi izitsha zabo ze-cryogenic azifanele ukucwiliswa esigabeni se-liquid. Uma lezi zitsha zigcinwe esigabeni esiwuketshezi lapho zibuyela ekamelweni lokushisa lezi zitsha noma ama-cap seal azo angase aphahlazeke ngenxa yokwakheka okusheshayo kokucindezela okubangelwa ukuvuza okuncane.
Uma amaseli azogcinwa esigabeni esiwuketshezi se-nitrogen ewuketshezi, cabanga ukugcina amaseli ezitsheni ezifanele ze-cryogenic ezivalwe ngokushisisa kushubhu we-cryoflex noma ukugcina amaseli kuma-ampule engilazi avalwe ngendlela eyisimanga.
Isikhathi sokuthumela: Nov-25-2022