Yini amaCryvials?
Ama-vials wesitoreji se-Cryogeniczizincane, eziboshwe futhi zinama-cylindrical eziklanyelwe ukugcina nokugcina amasampula emazingeni okushisa aphansi we-ultra. Yize ngokwesiko la ma-vials enziwe ngengilazi, manje ajwayele ukwenziwa nge-polypropylene ukuze kube lula futhi abe nezizathu zezindleko. AmaCryvials aklanyelwe ngokucophelela ukubekezelela amazinga okushisa aphansi as -196 ℃, kanye nokuhlalisa izinhlobo ezahlukahlukene zeselula. Lokhu kuyehluka kusuka ekuxilongeni amaseli, ama-microorganisms, amaseli aphambili ukuze asungulwe imigqa yamaseli. Ngaphandle kwalokho, kungahle kube nezinto ezincane ze-multicelleller ezigcinwe ngaphakathiAma-vials wesitoreji se-Cryogenic, kanye ne-nucleic acid namaprotheni adinga ukugcinwa emazingeni okushisa okushisa a-cryogenic.
Ama-vials wesitoreji se-Cryogenic eza ngezindlela ezahlukahlukene ezahlukahlukene, futhi athole uhlobo olufanele olugcwalisa zonke izidingo zakho ezoqinisekisa ukuthi ugcina ubuqotho besampula ngaphandle kokukhokha ngokweqile. Funda ngendatshana yethu ukuze ufunde kabanzi ngokucatshangelwa okusemqoka lapho ukhetha i-cryrovial efanele isicelo sakho selabhorethri.
Izakhiwo ze-crynic vial okufanele zicatshangelwe
Imicu yangaphakathi vs
Abantu bavame ukwenza lokhu kukhetho kususelwa ekuthandweni komuntu siqu, kepha empeleni kukhona umehluko obalulekile obalulekile ongasicubungula phakathi kwezinhlobo ezimbili zentambo.
Ama-laboratories amaningi avame ukukhetha ama-vials ahlanganiswe ngaphakathi ukunciphisa isikhala sokugcina ishubhu ukuvumela ukulingana okungcono kumabhokisi efriji. Ngaphandle kwalokhu, ungabheka ukuthi inketho engaphandle etholwayo iyindlela engcono kuwe. Babhekwa njengokuphatha ingozi yokungcola ephansi, ngenxa yomklamo owenza kube nzima kakhulu nganoma yini enye ngaphandle kwesampula yokungena kwi-vial.
Ama-vials angaphandle ahlanganisiwe ngokuvamile ancanyelwa izinhlelo zokusebenza ze-genomic, kepha noma iyiphi inketho ibhekwa njengezilungele i-biobanking nezinye izinhlelo zokusebenza eziphezulu.
Into eyodwa yokugcina okufanele uyicabangele ngokucophelela - Uma ngabe ilabhorethri yakho isebenzisa okuzenzakalelayo, kungadingeka ukuthi ucabangele ukuthi iyiphi intambo engasetshenziswa ngama-gripper wensimbi.
Ivolumu yokugcina
Ama-vials ahlukene atholakala ngosayizi ahlukahlukene ukumboza izidingo eziningi, kepha iningi bahluka phakathi komthamo we-1 ml no-5 ml.
Isihluthulelo ukuqiniseka ukuthi i-cryovial yakho ayigcwalisiwe nokuthi kukhona igumbi elengeziwe elikhona, uma kwenzeka kugcwaliswe isampula. Empeleni, lokhu kusho ukuthi ama-laboratories akhetha ama-vials angu-1 ml lapho egcina amasampula angu-0,5 ml wamaseli amiswe ku-cryproprectant, kanye nama-2.0 ml vents we-1.0 ml wesampula. Elinye ithiphu lokungagcwaliseli ama-vials akho ukukwenza usebenzise ama-cryvials anezimpawu ezithweswe iziqu, ezokwenza isiqiniseko sokuthi uvimbela noma yikuphi ukuvuvukala okungadala ukuqhekeka noma ukuvuza.
Screw cap vs flip top
Uhlobo oluphezulu okhethayo luncike kakhulu ekutheni uzobe usebenzisa i-nitrogen yesigaba se-liquid noma cha. Uma unjalo, uzodinga ama-cryvials ahlanganisiwe. Lokhu kuqinisekisa ukuthi ngeke bakwazi ukuphuma ngengozi ngenxa yokushintshana noma izinguquko zokushisa. Ngokwengeziwe, ama-screw cap avumela ukubuyisa okulula kusuka kumabhokisi e-crynic kanye nesitoreji esisebenza kahle.
Kodwa-ke, uma ungasebenzisi i-Liquid Stage Nitrogen futhi udinga phezulu okulula okulula ukuvula, khona-ke i-Flip Top iyindlela engcono. Lokhu kuzokusindisa isikhathi esiningi njengoba kulula kakhulu ukuvula, okwenza kube wusizo ikakhulukazi emisebenzini ephakeme ephakeme kanye nalawo asebenzisa izinqubo ze-batch.
Seal Security
Indlela engcono kakhulu yokuqinisekisa ukuthi uphawu oluvikelekile ukuqiniseka ukuthi iCryvial cap nebhodlela lakho lakhiwe ngezinto ezifanayo. Lokhu kuzoqinisekisa ukuthi bayancipha futhi banwebe ngelinye izwe. Uma benziwa besebenzisa izinto ezahlukene, bazoncipha futhi bandise ngamanani ahlukene njengoba kushintsha izinga lokushisa, izikhala eziholayo kanye nokungcola okungaba khona.
Ezinye izinkampani zinikeza ama-washers ama-ntambama kanye ne-flange yezinga eliphakeme kakhulu lokuphepha okuyisibonelo kuma-crycrials angaphandle ane-threaded. Ama-O-Ring Cryvials abhekwa njengethembeke kakhulu ngama-cryrials angaphakathi nambili.
Ipulasitiki yengilazi vs
Ukuze ukuphepha kanye lula, ama-laboratoratories amaningi manje asebenzisa ipulasitiki, imvamisa i-polypropylene, esikhundleni sama-amplece angenamanga ashisekile. Ama-amplusis e-Glass manje athathwa njengekhetho eliphelelwe yisikhathi ngangokunokwenzeka kwenqubo yokuvalwa kwe-PinHible Pinhole Ukuvuza, lapho kuncipha ngemuva kokugcinwa kwe-nitrogen ewuketshezi kungasenza baqhume. Futhi akulungele amasu wesimanjemanje wokulebula, okuyisihluthulelo sokuqinisekisa ukulandelwa kwesampula.
Ukuzimela vs vs ama-bottops ahlanganisiwe
Ama-Cryhic vials atholakala ngokuzenzela ngamabhodlela amise izinkanyezi, noma njengezinkomba eziyindilinga. Uma udinga ukubeka ama-vials akho ebusweni bese uqinisekisa ukukhetha ukuzimela
Ukulandelela kanye nokulandela isampula
Le ndawo yokugcina isitoreji i-cryogenic ivame ukunganakwa kepha ukulandelela isampula nokulandela umkhondo kuyisici esibalulekile okufanele sicabange. Amasampula ama-Crygenic angagcinwa iminyaka eminingi, lapho abasebenzi besikhathi abangashintsha khona futhi ngaphandle kwamarekhodi agcinwe kahle bangakwazi ukungazi.
Qiniseka ukuthi ukhetha ama-vials enza ukukhonjwa kwesampula kube lula ngangokunokwenzeka. Izinto okufanele uzibheke kuzo zifaka:
Izindawo ezinkulu zokubhala ukurekhoda imininingwane eyanele ukuze amarekhodi angatholakala uma i-vial itholakala endaweni engalungile - imvamisa ubunikazi beselula, usuku lokuqanjwa kweqhwa, kanye nokuqala komuntu obhekele kwanele.
Amabhakhodi wokusiza amasistimu wokuphatha amasampula nezinhlelo zokulandela umkhondo
Izimbotshana ezinemibala
Inothi yesikhathi esizayo - ama-chips amelana nokubanda okubandayo ayakhiwa, lapho efakwe ngaphakathi kwamaCrmavials, angagcina umlando onemininingwane we-Thermal kanye nemininingwane enemininingwane ye-batch, imiphumela yokuhlola kanye neminye imibhalo efanelekile.
Ngaphezu kokuthola ukucatshangelwa kwencazelo ehlukile yama-vials atholakalayo, eminye imicabango nayo idinga ukunikezwa inqubo yezobuchwepheshe yokugcina ama-cryrogen awuketshezi.
Ukushisa okushisa
Kunezindlela eziningana zokugcina zesitoreji se-cryogenic samasampula, ngalinye lisebenza ngamazinga okushisa athile. Izinketho kanye nezinga lokushisa abalisebenza kuhlanganisa:
Isigaba se-Liquid LN2: Gcina izinga lokushisa le -196 ℃
Isigaba se-Vapor Isigaba se-LN2: Iyakwazi ukusebenza kumabanga athile okushisa phakathi kwango-115 ° C no -190 ° C kuya ngemodeli.
I-Nitrogen Vapor Freezers: -20 ° C kuya ku -150 ° C
Uhlobo lwamaseli agcinwe kanye nendlela yokulondolozwa yomcwaningi ekhethwayo kuzonquma ukuthi yikuphi okukhethwa kukho okutholakalayo okutholakalayo kwelabhoratri okusebenzisayo.
Kodwa-ke, ngenxa yamazinga okushisa aphansi kakhulu aqashelwe hhayi wonke amashubhu noma ama-design azofaneleka noma aphephile. Izinto zokwakha zingaba amazinga okushisa aphansi kakhulu, kusetshenziswa i-vial engafanele ukusetshenziswa ekushiseni kwakho okukhethiwe kungadala ukuthi umkhumbi ushaye noma uqhekeke ngesikhathi sokugcina noma ukuqhekeka.
Hlola ngokucophelela izincomo zabakhiqizi ngokusetshenziswa kahle njengoba ezinye izinhlobo ze-cryogenic zilungele amazinga okushisa aphansi as -175 ° C, amanye ama-c, amanye ama-80 ° C.
Kuhle futhi ukuqaphela ukuthi abakhiqizi abaningi bathi ama-vials abo ama-cryogenic awalungele ukucwiliswa esigabeni se-liquid. Uma lezi zinhlamvu zigcinwa esigabeni se-liquid lapho zibuyela ekushiseni kwegumbi lezi zinhlamvu noma uphawu lwazo lwe-cap zingaphazamisa ngenxa yokwakhiwa okusheshayo kwengcindezi ebangelwa ukuvuza okuncane okubangelwa ukuvuza okuncane.
Uma amaseli kufanele agcinwe esigabeni se-liquid se-nitrogen ewuketshezi, cabanga ngokugcina amaseli ezintweni ezifanele ze-cryogenic vials-livalwe uphawu ku-crCloglex tubing noma ukugcinwa kwamangqamuzana ema-amplusis avaliwe.
Isikhathi sePosi: Nov-25-2022