Siqinisekisa kanjani ukuthi imikhiqizo yethu ayinawo i-DNase RNase futhi ivalwa kanjani amagciwane?

Kwa-Suzhou Ace Biomedical, siyaziqhenya ngokuhlinzeka ngezinto ezisebenzisekayo zaselabhorethri ezisezingeni eliphezulu kubacwaningi nososayensi emhlabeni jikelele. Ukuzibophezela kwethu ekwenzeni okuhle kusishukumisela ukuba siqinisekise ukuthi imikhiqizo yethu ayinakho noma yikuphi ukungcola okungase kuthinte imiphumela yokuhlolwa. Kulesi sihloko, sixoxa ngezinyathelo eziqinile esizithathayo ukuze siqinisekise ukuthi imikhiqizo yethu ayinayo i-DNase-RNase, kanye nenqubo yokuvala inzalo ebhekana nayo.

I-DNase ne-RNase ama-enzyme alulaza ama-nucleic acid, okungama-molecule abalulekile ahilelekile ezinqubweni ezihlukahlukene zezinto eziphilayo. Ukungcola kwe-DNase noma kwe-RNase kungaba nomthelela omubi ekuhlolweni, ikakhulukazi lokho okubandakanya ukuhlaziywa kwe-DNA noma i-RNA okufana ne-PCR noma ukulandelana kwe-RNA. Ngakho-ke, kubalulekile ukuqeda noma yimiphi imithombo engaba khona yalawa ma-enzyme ezintweni ezisetshenziswa elabhorethri.

Ukuze sithole isimo se-RNase engena-DNase, sisebenzisa amasu amaningi kuzo zonke izigaba zenqubo yokukhiqiza. Okokuqala, siqinisekisa ukuthi izinto zethu zokusetshenziswa zingezeqophelo eliphezulu futhi azingcoliswanga yi-DNase RNase. Inqubo yethu ephelele yokukhetha abahlinzeki ihilela ukuhlolwa okuqinile nokuhlolwa ukuze kuqinisekiswe ukuthi izinto ezihlanzekile kuphela ezifakiwe emikhiqizweni yethu.

Ngaphezu kwalokho, silandela izindlela eziqinile zokukhiqiza kanye nezinyathelo zokulawula ikhwalithi ezindaweni zethu zokukhiqiza. Isikhungo sethu sokukhiqiza esisezingeni eliphezulu siqinisekisiwe nge-ISO13485, okusho ukuthi silandela izindinganiso zesistimu yokuphatha ikhwalithi eyaziwa emhlabeni wonke. Lesi sitifiketi asiqinisekisi kuphela ikhwalithi yemikhiqizo yethu, kodwa sibonisa ukuzibophezela kwethu ekuthuthukiseni okuqhubekayo nokwaneliseka kwamakhasimende.

Ukuze uvimbele ukungcoliswa kwe-DNase RNase ngesikhathi sokukhiqiza, sisebenzisa uchungechunge lwezinqubo zokuqeda ukungcola. Imishini yethu, okuhlanganisa namathiphu e-pipette namapuleti ajulile, ingena ezinyathelweni eziningi zokuhlanza nokuvala inzalo. Sisebenzisa ubuchwepheshe obuthuthukisiwe obufana ne-autoclaving kanye ne-electron beam sterilization ukuze sinikeze ukuvala inzalo okusebenza kahle kakhulu kuyilapho sigcina ubuqotho bezinto ezibonakalayo.

I-Autoclaving iyindlela esetshenziswa kakhulu yokubulala inzalo izinto ezisetshenziswa elabhorethri. Kuhilela ukufaka umkhiqizo ngaphansi kwesitimu esigcwele ingcindezi ephezulu, eqeda ngempumelelo noma yimaphi ama-microorganisms, okuhlanganisa i-DNase ne-RNase. Kodwa-ke, ezinye izinto ezisetshenziswayo zingase zingafaneleki ukwenza i-autoclaving ngenxa yezakhiwo zazo ezibonakalayo. Kulesi simo, sisebenzisa ukuvalwa kwe-e-beam, okusebenzisa i-electron enamandla amakhulu ukuze kufezwe inzalo. Ukuvala inzalo ye-electronic beam kunokusebenza kahle okuphezulu, akuncikile ekushiseni, futhi kulungele ukuvalwa inzalo kwezinto ezingezwani nokushisa.

Ukuqinisekisa ukusebenza kahle kwezindlela zethu zokuvala inzalo, sihlala siqapha futhi siqinisekisa izinqubo zethu. Senza ukuhlola kwe-microbiological ukuze siqinisekise ukungabikho kwamagciwane aphilayo, okuhlanganisa i-DNase ne-RNase. Lezi zinqubo zokuhlola eziqinile zisinika ithemba lokuthi imikhiqizo yethu ayinakho ukungcola okungaba khona.

Ngaphezu kwezinyathelo zethu zangaphakathi, senza nokuhlola okuzimele ngokubambisana namalabhorethri ahlonishwayo ezinkampani zangaphandle. Lezi zindawo zokuhlola zangaphandle zisebenzisa amasu azwela kakhulu ukuhlola imikhiqizo yethu ukungcoliswa kwe-DNase RNase futhi zingathola ngisho nokulandelela amanani alawa ma-enzyme. Ngokufaka imikhiqizo yethu ngaphansi kwalezi zivivinyo ezinzima, singaqinisekisa amakhasimende ethu ukuthi athola izinto ezisetshenziswa elabhorethri zekhwalithi ephezulu kakhulu nezingangcoli.

At I-Suzhou Ace Biomedical, ukuzibophezela kwethu kukhwalithi nokwaneliseka kwamakhasimende kusishukumisela ukuba siqinisekise ukuthi imikhiqizo yethu ayinayo i-DNase futhi ayinayo i-RNase. Kusukela ekukhethweni ngokucophelela kwezinto zokusetshenziswa kuye ekusetshenzisweni kwezindlela ezithuthukisiwe zokuvala inzalo, asigodli umzamo othile ekuphishekeleni kwethu ukwenza kahle. Ngokukhetha imikhiqizo yethu, abacwaningi bangaba nethemba ekuthembekeni nasekunembeni kwemiphumela yabo yokuhlola, ekugcineni basheshise inqubekelaphambili yesayensi.