96-Well Plate Plate (Ipuleti ejulile) luhlobo lwepuleti elisebenziseka kahle elivame ukusetshenziswa ezindaweni zokucwaninga. Inomklamo ojulile umgodi futhi ivame ukusetshenziselwa izivivinyo ezidinga amanani amakhulu amasampula noma ama-reagents. Lokhu okulandelayo kungenye yezicelo ezinkulu zohlelo lokusebenza kanye nezindlela zokusebenzisa amapuleti ajulile ajulile angu-96:
Uhla lwesicelo:
Ukuhlolisisa okuphezulu okuthi: Ezihlolweni ezinjengokuhlola izidakamizwa kanye nokuhlola umtapo wezincwadi, amapuleti ajulile ajulile ajulile angathola ama-Samples amaningi futhi athuthukise ukusebenza kahle kokuhlola.
Isiko Leseli: Ilungele izivivinyo zesiko leseli ezidinga umthamo omkhulu wamasiko aphakathi, ikakhulukazi isiko lamaseli anamathela.
I-Enzyme-ixhumeke i-Imunosorbent Assay (ELISA): Isetshenziswa ekuhlolweni kwe-ELISA edinga umthamo omkhulu wohlelo lokuphendula.
Ukuhlolwa kwe-molecular biology: njengokuphendula kwe-PCR, i-DNA / RNA Extraction, I-Electrophoresis Sampula Ukulungiswa, njll.
I-Protein Expression kanye nokuhlanzwa: Kusetshenziswe ezivivinyweni ezinenkulumo enkulu yamaprotheni noma kudinga inani elikhudlwana le-buffer.
Isitoreji sesampula yesikhathi eside: Ngenxa yokujula komgodi omkhulu, ukushintshwa kwevolumu yesampula ngesikhathi sokuqanda kungancishiswa, okulungele ukugcinwa isikhathi eside.
Indlela yokusebenzisa:
Ukulungiswa kwesampula: Ngokwezidingo zokuhlolwa, ukukala ngokunembile inani elifanele lesampula noma i-realent bese kwengeza emthonjeni wepuleti elijulile.
Ukubekwa uphawu
Ukuxuba: Shakeza ngobumnene noma usebenzise i-Multichannel Pipette ukuxuba isampula ukuze uqinisekise ukuthi isampula liyaxhumana ngokuphelele ne-reagent.
Ukufakwa: Beka ipuleti elijulile ebhokisini lokushisa elihlala njalo noma elinye indawo efanelekile yokufakwa ngokusho kwezidingo zokuhlola.
Idatha yokufunda: Sebenzisa amathuluzi afana nabafundi baka-microplate kanye nama-Weliorescence Microscopes ukufunda imiphumela yokuhlola.
Ukuhlanza nokubulala amagciwane: Ngemuva kokuhlolwa, sebenzisa ama-detegergents afanele ukuhlanza ipuleti elijulile futhi alisusele amagciwane.
Isitoreji: Ipuleti elijulile kufanele ligcinwe kahle ngemuva kokuhlanza kanye nokubulala amagciwane ukugwema ukungcoliswa.
Lapho usebenzisa amapuleti ajulile angama-96, amaphuzu alandelayo kufanele futhi aphawulwe:
Ukucaciswa kokusebenza: Landela ukucaciswa kokusebenza kwe-asuptic ukugwema ukungcoliswa kwesampula.
Ukunemba: Sebenzisa i-multichannel pipette noma uhlelo lokuphatha othomathikhi ukuthuthukisa ukunemba kokusebenza.
Ukumakekwa okucacile: Qiniseka ukuthi umthombo ngamunye wepuleti kahle umaki ngokusobala ukuze ukhombe futhi uqopha.
96-kahle ngokujulile-kahleAmapuleti ayithuluzi elibalulekile lokuhlola okuphezulu kwelebhu. Ukusetshenziswa okufanele kungathuthukisa kakhulu ukusebenza kahle nokunemba kokuhlolwa.
Isikhathi sePosi: Aug-13-2024