Ipuleti lomthombo elijulile elingu-96 (I-Deep Well Plate) wuhlobo lwepuleti elinemithombo eminingi elivame ukusetshenziswa ezindaweni zokucwaninga. Inomklamo wembobo ejulile futhi ivamise ukusetshenziselwa ukuhlola okudinga amavolumu amakhulu amasampula noma ama-reagents. Okulandelayo ezinye zezinhlelo eziyinhloko nezindlela zokusetshenziswa zamapuleti emithombo ejulile engu-96:
Ibanga lesicelo:
Ukuhlolwa komphumela ophezulu: Ezivivinyweni ezifana nokuhlolwa kwezidakamizwa nokuhlolwa komtapo wezincwadi okuhlanganisiwe, amapuleti emithombo ejulile angu-96 angakwazi ukwamukela amasampula engeziwe futhi athuthukise ukusebenza kahle kokuhlolwa.
Isiko leseli: Ifanele ukuhlolwa kwesiko lamaseli okudinga umthamo omkhulu wendlela yesiko, ikakhulukazi isiko lamaseli abambelelayo.
I-Enzyme-linked immunosorbent assay (ELISA): Isetshenziswa ekuhloleni kwe-ELISA okudinga umthamo omkhulu wesistimu yokusabela.
Ukuhlolwa kwebhayoloji yamangqamuzana: Okufana nokusabela kwe-PCR, ukukhishwa kwe-DNA/RNA, ukulungiswa kwesampula ye-electrophoresis, njll.
Ukubonakaliswa kwephrotheni nokuhlanzwa: Kusetshenziswa ekuhlolweni okuveza amaprotheni amakhulu noma kudinga umthamo omkhulu webhafa.
Isitoreji sesampula sesikhathi eside: Ngenxa yokujula kwembobo enkulu, ukuguqulwa kwevolumu yesampula ngesikhathi sokuqandisa kungancishiswa, okufanele ukugcinwa isikhathi eside.
Indlela yokusebenzisa:
Ukulungiselela isampula: Ngokuvumelana nezidingo zokuhlolwa, linganisa ngokunembile inani elifanele lesampula noma i-reagent bese uyengeza emthonjeni wepuleti lomthombo ojulile.
Ukuvala: Sebenzisa ifilimu evalwayo efanelekile noma igasket ukuze uvale ipuleti lomthombo ukuvimbela ukuhwamuka kwesampula noma ukungcola.
Ukuxuba: Nyakazisa ngobumnene noma usebenzise i-pipette ye-multichannel ukuze uhlanganise isampula ukuze uqinisekise ukuthi isampula ixhumene ngokugcwele ne-reagent.
Ukufukamela: Beka ipuleti elijulile lomthombo ebhokisini lokushisa elingashintshi noma enye indawo efanelekile yokufukamela ngokwezidingo zokuhlola.
Ukufunda idatha: Sebenzisa amathuluzi afana nezifundi ze-microplate nama-fluorescence microscopes ukuze ufunde imiphumela yokuhlolwa.
Ukuhlanza nokubulala amagciwane: Ngemva kokuhlolwa, sebenzisa okokugeza okufanelekile ukuze uhlanze ipuleti elijulile lomthombo futhi ubulale amagciwane.
Isitoreji: Ipuleti elijulile kufanele ligcinwe kahle ngemva kokuhlanza nokubulala amagciwane ukuze kugwenywe ukungcoliswa.
Uma usebenzisa amapuleti ajulile angu-96, kufanele kuqashelwe amaphuzu alandelayo:
Ukucaciswa komsebenzi: Landela ukucaciswa kokusebenza kwe-aseptic ukuze ugweme ukungcoliswa kwesampula.
Ukunemba: Sebenzisa i-pipette ye-multichannel noma isistimu yokuphatha uketshezi oluzenzakalelayo ukuze uthuthukise ukunemba komsebenzi.
Ukumaka okucacile: Qiniseka ukuthi umthombo ngamunye wepuleti lomthombo umakwe ngokucacile ukuze ukhonjwe kalula futhi urekhodwe.
96-ujule kahleamapuleti ayithuluzi elibalulekile lokuhlolwa komphumela ophezulu elabhorethri. Ukusebenzisa kahle kungathuthukisa kakhulu ukusebenza kahle nokunemba kokuhlolwa.
Isikhathi sokuthumela: Aug-13-2024