I-micropipete cishe iyithuluzi elisetshenziswa kakhulu elabhorethri. Basetshenziswa ososayensi emikhakheni ehlukahlukene kufaka phakathi i-academia, isibhedlela kanye ne-labs ye-forensics kanye nokuthuthukiswa kwezidakamizwa ukudlulisa amanani aqondile, amancane kakhulu
Ngenkathi kungaba ukucasuka futhi kukhungathekise ukubona ama-bubble e-Spot Air ku-Pipette Pipette elahlekile uma kungaboni noma kunganakwa kungathonya elikhulu ekuthembekeni nasekudayisweni kwemiphumela.
Izindaba ezimnandi ukuthi kunezindlela ezithile ezilula ongazithatha ukuvikela ama-bubble amoya, futhi uthuthukise ukusebenza kahle kwelebhu, ukwaneliseka komshayeli kanye nokunemba kwemiphumela.
Ngezansi, sihlola umphumela wokuthola i-bubble yomoya ku-pipette yakho ye-pipette nokuthi yini okufanele uyenze ngokulandelayo.
Umphumela wama-bubble kuIthiphu yePipette
Noma ngabe usebenzisa okunembile kakhulu, okuphezulu kwebanga, okugcizelelwa kahle, ama-Pipete asetshenziswayo futhi ahlinzekiwe futhi ahlanganisiwe ukuthembeka kwezemiphumela yakho kungathinteka ngamaphutha welebhu. Lapho ama-bubble engena kuuthiphuKungaba nemiphumela eminingana.
● Lapho umsebenzisi ebona i-bubble yomoya kumele achithe isikhathi esabalalisa uketshezi olufisayo ngendlela efanele, khipha ithiphu bese uqala inqubo futhi.
● Ama-bubble emoya angabonakali angahle aholele ekudluliseleni ngevolumu ephansi, ngaleyo ndlela aguqule ukuhlanganiswa kwezingxube zokuphendula eziholela ekuhlolweni okuhlulekile kanye nemiphumela engabazekayo noma engathembekile.
Le miphumela ingaba nemiphumela eminingana (1).
● Ukuncipha kwelebhu Ukusebenza kweLab
● imiphumela yokuhlolwa engabazekayo noma engalungile - uma imiphumela engalungile ikhishwa kungaba nemiphumela emibi kakhulu kufaka phakathi i-misdiagnosis kanye nemiphumela yeziguli empofu.
● Ukubuyiselwa kwemibhalo yesandla kusuka kumaphephabhuku - uma ontanga behluleka ukuphindaphinda imiphumela yakho ngenxa yama-bubble omoya abangela ukuthi amaphepha wemiphumela engalungile angahoxiswa.
Imikhuba emihle kakhulu yokuvimbela ama-bubble amoya
Esikhathini esiningi samacala ama-air bubble kumathiphu wePipette abangelwa yiphutha le-opharetha. Indlela empofu ngenxa yokuqeqeshwa okunganele noma ukukhathala imvamisa yinkinga eyisisekelo.
I-Pipetting ngumsebenzi onekhono okudinga ukunakwa ngo-110%, ukuqeqeshwa okufanele futhi uzijwayeze ukufeza imiphumela engaguquguquki futhi enembile.
Ngenkathi kunezinto eziningana ongazenza ukuze wehlise amaphutha ajwayelekile wePipet, ngezansi esiveza eminye imikhuba emihle engasetshenziswa ukugwema ama-bubble air inAmathiphu wePipette.
Thuthukisa inqubo yomsebenzisi
I-Pipette kancane
Uma i-plunger ikhishwe ngokushesha okukhulu lapho ifisa, ama-bubble omoya angeniswa esiqeshini. Lokhu kungaba yinkinga ikakhulukazi lapho kudluliswa uketshezi lwe-viscous. Umphumela ofanayo kungenzeka uma i-plunger ikhishwe ngokushesha ngemuva kokusabalalisa.
Ukugwema ama-bubble omoya lapho ufisa, nakekela ukusebenzisa i-piston yamapayipi ebhukwana ngendlela ebushelelezi futhi ejwayelekile, isebenzisa amandla angaguquki.
Sebenzisa ukujula okuphelele kokucwilisa
Ukwehluleka ukucwilisa ithiphu ye-pipette ejulile ngokwanele ngezansi kwe-meniscus ye-liquid reservoir ingaholela ekutheni isifiso somoya futhi ngaleyo ndlela ukwakheka kwe-bubble.
Kodwa-ke, ukucwilisa isicoco kakhulu kungafisa kakhulu uketshezi ngenxa yengcindezi eyengeziwe noma amaconsi kungenzeka ngaphandle kwethiphu ngakho-ke kubalulekile ukucwilisa iIthiphu yePipetteekujuleni okuyikho.
Ukujula okunconyiwe kuyehlukahluka phakathi kukasayizi we-pipette, thayipha nokwenza. Ngenkathi izincomo zomkhiqizi kufanele zilandelwe nangu umhlahlandlela jikelele onikezwe ilabhorethri kazwelonke.
Umhlahlandlela wokujula kokucwiliswa kwe-tip
I-Pipette Volume (μL) nokujula kokucwiliswa (mm)
- 1 - 100: 2 - 3
- 100 - 1 000: 2 - 4
- I-1,000 - 5,000: 2 - 5
I-Pre-WetAmathiphu wePipette
Lapho iPipetting Volumes inkulu kune-10μLAmathiphu wePipettezivame ukwenziwa ngaphambili ngokuzigcwalisa kaningana ngoketshezi olusatshalaliswa futhi lukuxosha ukuchitha ukuze kuthuthukiswe ukunemba.
Ukwehluleka ku-pre-wet kubo kungaholela kuma-bubble amoya, ikakhulukazi lapho usebenzisa i-viscous noma i-hydrophobic ketshezi. Ukugwema ama-Bubble Air Qinisekisa ukuthi amathiphu we-pre-wet lapho ivolumu yepayipi likhulu kune-10μL.
Sebenzisa amasu okubuyela emuva wePipetting uma kufanele
Izinto ze-Viscous: Inkinga evamile lapho i-Pipetting e-vipetting discous efana namaprotheni noma izixazululo ze-nucleic acid, i-12/40/60/80 isetshenziswa ama-Bubbles lapho kusetshenziswa inqubo ye-Pipetting.
I-Pipetting kancane, usebenzisa inqubo yokubuyela emuva ye-Pipetting inciphisa ubungozi bokwakheka kwe-bubble lapho kudluliswa izixazululo ze-viscous.
Isu le-ELISA
Ukubuyela emuva kwe-Pipetting nakho kunconyiwe lapho kufakwa ama-pripetting amancaneAmapuleti wokuhlola amancane ayi-96amasu e-ELISA. Lapho ama-bub Bub Bubble adonswa ku-pipette noma asatshalaliswa emithonjeni lapho engeza ama-reagents angathonya amanani wokuhlanya obona kanye nemiphumela. I-Reverse Pipetting inconywa ukunciphisa noma ukuqeda lolu daba.
Sebenzisa ama-Pipete e-ergonomic
Izitayela ezindala zesitayela ezingazange zenzelwe ama-ergonomics engqondweni zidinga umzamo owengeziwe womzimba, ukhathala futhi inqubo yakho yePipetting iba sloppy futhi imbi. Amaphutha ashiwo ngenhla njengokukhishwa kwe-Planger esheshayo kungenzeka kaningi.
Ngokutshala imali kwikhambi le-ergonomic ngaphezulu uzokwazi ukugcina inqubo enhle kakhulu futhi uvikele ukwakheka kwama-bubble air ngenxa yenqubo engeyona.
Zinike isikhathi sokuqeqesha abasebenzi
Ukuqeqeshwa njalo nokuhlola abasebenzi emasu e-Pipetting kungaqinisekisa ukuthi iphutha le-opharetha kanye nokwakheka kwe-air bubble kuncishisiwe.
Cabanga ngezixazululo ezenzakalelayo ezenzakalelayo
Njengoba kuphawulwe ngenhla kwama-bubble amaningi amoya kubangelwa opharetha. Kungenzeka kwenzeke ukwehlisa iphutha le-opharetha nenduduzo ngokusebenzisa ama-Pipette e-elekthronikhi noma ipulatifomu yokuphatha uketshezi eguqukayo efana neI-agilent bravo liquid robot yokuphatha.
Sebenzisa ikhwalithi enhleAmathiphu wePipette
AmaMicropipette avame ukuthengiwe ngokunakekelwa, kepha kaningi kucatshangelwa ikhwalithi ye-Pipette elahlekile ye-PIPETETE. Ngenxa yethonya ithiphu elinalo emiphumeleni yePipetting, i-CalleStiso 8655 idinga ukulinganisa okwengeziwe uma amapayipi namathiphu avela kubakhiqizi abahlukene asetshenziswa.
Lokhu kungaba ngoba amathiphu amaningi ashibhile angabukeka kahle ekuqaleni kepha uma ufunda ngokucophelela kungenzeka ukuthi kungenzeka ukuthi avele, ama-protors, ama-bubble ama-air, noma aqukethe ukungcola.
Ukuthenga amathiphu amahle wekhwalithi eyenziwe nge-polypropylene ephezulu enganciphisa ukwenzeka kwama-bubble amoya.
Ukuphetha
Ukuthola ama-bubble omoya ku-pipette yakho ye-pipette kunomthelela ekusebenzeni kahle kwelebhu kanye nokunganembile kanye nokungafanele imiphumela. Siphawule izinto eziningana ongazenza ukugwema ama-bubble omoya ukungena kuIthiphu yePipette.
Kodwa-ke, uma ikhwalithi engeyinhleAmathiphu wePipettezibangela ama-bubble omoya ukungena ephuze lakho le-pipette, uzokujabulela ukwazi ukuthi kufanelekile ukuthi kube semhlabeniAmathiphu wePipettezenziwa kumazinga aphezulu kakhulu futhi zenziwa nge-premium-grade polypropylene.
Inkampani ye-suzhou ace biomedicalKhiqiza izinga eliphezulu-10,20,100,100,200,200,200,100,1000,150,1000 kanye nama-1250 μL Vvolume Universal Pipette Amathiphu, amathiphu angama-96 / rack. Ukuqina okuhlukile - wonke ama-racks we-ace tip tip asukume aze afinyelele ezifunweni zokusebenzisa nge-multichannel Pipettors. I-Erembile, hlunga, i-rnase- / dnase-free, ne-nonpogenic.
Uyemukelwa ukuze ubuze imininingwane eminingi.
Isikhathi Sokuthumela: Dec-29-2022