I-micropipette cishe iyithuluzi elisetshenziswa kakhulu elabhorethri. Asetshenziswa ososayensi emikhakheni eyahlukene ehlanganisa ezezemfundo, izibhedlela kanye namalebhu e-forensics kanye nokuthuthukiswa kwezidakamizwa nokugoma ukuze kudluliselwe inani eliqondile, elincane kakhulu loketshezi.
Nakuba kungase kucasule futhi kukhungathekise ukubona amabhamuza omoya ephuzwini le-pipette elilahlwayo uma engabonakali noma enganakwa kungaba nomthelela omkhulu ekuthembekeni nasekukhiqizeni kabusha imiphumela.
Izindaba ezinhle ukuthi kunezinyathelo ezilula ongazithatha ukuze uvimbele amabhamuza omoya, futhi uthuthukise ukusebenza kahle kwelebhu, ukwaneliseka komqhubi kanye nokunemba nokunemba kwemiphumela.
Ngezansi, sihlola umphumela wokuthola ibhamuza lomoya ithiphu lakho le-pipette nokuthi yini okufanele uyenze ngokulandelayo.
Umphumela wama-Bubbles ku-Ithiphu ye-Pipette
Ngisho noma usebenzisa ama-pipette anembe kakhulu, angaphezulu kobubanzi, anakekelwe kahle, anesevisi futhi alinganisiwe ukwethembeka kwemiphumela yakho kungathinteka ngamaphutha elebhu. Lapho ama-bubbles engenaithiphuingaba nemiphumela eminingana.
● Uma umsebenzisi ebona ibhamuza lomoya kufanele achithe isikhathi ekhipha uketshezi olushiswayo ngendlela efanele, akhiphe ithiphu bese eqala inqubo futhi.
● Amabhamuza omoya angatholakali angase abangele ukudluliswa kwevolumu ephansi, ngaleyo ndlela aguqule ukuhlangana kwezingxube zokusabela okuholela ekuhloleni okuhlulekile kanye nemiphumela engabazekayo noma engathembeki.
Le miphumela ingaba nemiphumela embalwa (1).
● Ukuncipha Kwempumelelo Yelebhu - Ukuhlolwa nokuhlolwa kuyodingeka kuphindwe, kube nezindleko zomsebenzi kanye nezinto ezibonakalayo, ezingaba nkulu kakhulu.
● Imiphumela Yokuhlola Engabazekayo noma Engalungile - Uma kukhishwa imiphumela engalungile kungase kube nemiphumela emibi kakhulu ehlanganisa ukuxilonga okungalungile kanye nemiphumela emibi yesiguli.
● Ukuhoxiswa Kwemibhalo Yesandla Kumajenali - Uma ontanga behluleka ukuphindaphinda imiphumela yakho ngenxa yamabhamuza omoya abangela ukuthi amaphepha emiphumela angalungile angahoxiswa.
Izindlela Ezingcono Kakhulu Zokuvimbela Amabhamuza Omoya
Ezimweni eziningi amabhamuza omoya kumathiphu we-pipette abangelwa iphutha lomqhubi. Inqubo engalungile ngenxa yokuqeqeshwa okunganele noma ukukhathala ngokuvamile yiyona nkinga ewumsuka.
Ukushaya ngamapayipi kuwumsebenzi onekhono odinga ukunakwa okungu-110%, ukuqeqeshwa okufanele nokuzijwayeza ukuze kuzuzwe imiphumela engaguquki nenembile.
Nakuba kunezinto ezimbalwa ongazenza ukuze unciphise amaphutha amapayipi avamile, ngezansi sigqamise izinqubo ezingcono kakhulu ezingasetshenziswa ukugwema amabhamuza omoyaamathiphu we-pipette.
Thuthukisa Indlela Yomsebenzisi
Pipette Kancane
Uma i-plunger ikhishwa ngokushesha kakhulu lapho ifisa, amabhamuza omoya angangeniswa esihlokweni. Lokhu kungaba yinkinga ikakhulukazi uma udlulisela uketshezi lwe-viscous. Umphumela ofanayo ungenzeka uma i-plunger ikhishwa ngokushesha kakhulu ngemva kokuyikhipha.
Ukuze ugweme amabhamuza omoya lapho ufisa, qaphela ukusebenzisa i-piston yamapayipi okwenziwa ngesandla ngendlela ebushelelezi futhi evamile, usebenzisa amandla angaguquki.
Sebenzisa Ukujula Okulungile Kokucwiliswa
Ukwehluleka ukucwilisa ithiphu le-pipette lijule ngokwanele ngaphansi kwe-meniscus ye-liquid reservoir kungabangela ukufisa komoya futhi ngaleyo ndlela ukwakheka kwamabhamuza.
Kodwa-ke, ukucwilisa ithiphu lijule kakhulu kungafisa uketshezi olwengeziwe ngenxa yomfutho ophakeme noma amaconsi angenzeka ngaphandle kwethiphu ngakho-ke kubalulekile ukucwilisaithiphu ye-pipetteekujuleni okulungile.
Ukujula okunconywayo kuyahlukahluka phakathi kobukhulu be-pipette, uhlobo nokwenza. Nakuba izincomo zomkhiqizi kufanele zilandelwe nansi inkomba ejwayelekile ehlinzekwa yi-National Physical Laboratory.
Umhlahlandlela Wokujula Kokucwiliswa Kwethiphu
Ivolumu ye-Pipette (µl) Nokujula Kokucwiliswa (mm)
- 1 - 100: 2 - 3
- 100 – 1,000: 2 – 4
- 1,000 - 5,000: 2 - 5
Ngaphambi KokumanziAmathiphu we-Pipette
Uma ivolumu yepayipi ingaphezu kuka-10µlamathiphu we-pipettezivame ukumanziswa ngaphambili ngokuzigcwalisa izikhathi eziningana ngoketshezi olukhishwayo futhi lukhishwe ukuze lumosheke ukuze kuthuthukiswe ukunemba.
Ukwehluleka ukuwamanzisa kusengaphambili kungase kubangele amabhamuza omoya, ikakhulukazi uma usebenzisa uketshezi lwe-viscous noma lwe-hydrophobic. Ukuze ugweme amabhamuza omoya qinisekisa ukuthi unamathiphu okumanzisa ngaphambili uma ufaka ivolumu enkulu kuno-10µl.
Sebenzisa Amasu Okuhlehla Amapayipi Uma Kufanele
I-Viscous Substances: Inkinga evamile lapho kufakwe amapayipi izinto ze-viscous ezifana ne-protein noma i-nucleic acids solutions, i-glycerol kanye ne-Tween 20/40/60/80 ukwakheka okuvamile kwamabhamuza lapho kusetshenziswa indlela yokuya phambili yamapayipi.
Ukubhobhoza kancane, ukusebenzisa indlela yokuhlehla yamapayipi kunciphisa ubungozi bokwakheka kwebhamuza lapho kudluliselwa izixazululo ze-viscous.
I-ELISA Technique
I-reverse pipetting nayo iyanconywa lapho ufaka amavolumu amancane phakathi96 kahle micro test platengamasu we-ELISA. Uma ama-bubbles omoya edonselwa ku-pipette noma asakazwa emithonjeni lapho engeza ama-reagents angathonya amanani we-optical density kanye nemiphumela. Ukuhlehla kwamapayipi kuyanconywa ukuze kuncishiswe noma kuqedwe le nkinga.
Sebenzisa i-Ergonomic Pipettes
Amapayipi esitayela esidala angazange aklanywe nge-ergonomics engqondweni adinga umzamo owengeziwe womzimba, uyakhathala futhi indlela yakho yokufaka amapayipi iba budlabha futhi ibe mpofu. Amaphutha ashiwo ngenhla njengokukhishwa kwe-plunger okusheshayo kungenzeka kaningi.
Ngokutshala imali kusixazululo se-ergonomic eyengeziwe uzokwazi ukugcina indlela enhle kakhulu futhi uvimbele ukwakheka kwamabhamuza omoya ngenxa yesu elibi.
Thatha Isikhathi Sokuqeqesha Abasebenzi
Ukuqeqesha njalo nokuhlola abasebenzi kumasu okufaka amapayipi kungaqinisekisa ukuthi iphutha lomqhubi kanye nokwakheka kwamabhamuza omoya kuyancishiswa.
Cabangela Izixazululo Ezizenzakalelayo Eziningi
Njengoba kuphawuliwe ngenhla amabhamuza amaningi omoya abangelwa u-opharetha. Kungase kwenzeke ukunciphisa iphutha lomsebenzisi nokunethezeka ngokusebenzisa amapayipi kagesi noma ipulatifomu yokubamba uketshezi efanaI-Agilent Bravo Liquid Handling Robot.
Sebenzisa Ikhwalithi EnhleAmathiphu we-Pipette
Ama-micropipettes avame ukuthengwa ngokucophelela, kodwa ngokuvamile umcabango omncane unikezwa ikhwalithi yethiphu ye-pipette elahlayo. Ngenxa yethonya ithiphu elinalo emiphumeleni yokufaka amapayipi, i-standardISO 8655 idinga ukulinganiswa okwengeziwe uma kusetshenziswa amapayipi namathiphu avela kubakhiqizi abahlukene.
Lokhu kungenxa yokuthi amathiphu amaningi ashibhile angabukeka ekahle ekuqaleni kodwa uma uwatadisha ngokucophelela angase abe nokubaneka, ama-protrusions, imihuzuko, namabhamuza omoya, noma agobe noma aqukathe ukungcola.
Ukuthenga amathiphu ekhwalithi enhle eyenziwe nge-polypropylene yezinga eliphezulu kungase kunciphise ukwenzeka kwamabhamuza omoya.
Sengiphetha
Ukuthola amabhamuza omoya ithiphu lakho le-pipette kunomthelela ekusebenzeni kahle kwelebhu kanye nokunemba nokungalungi kwemiphumela. Siqaphele izinto ezimbalwa ongazenza ukuze ugweme amabhamuza omoya ukungenaithiphu ye-pipette.
Nokho, uma izinga eliphansiamathiphu we-pipetteabangela amabhamuza omoya ukuthi angene ithiphu lakho le-pipette, uzojabula ukwazi ukuthi ukulingana kwethu kwendawo yonkeamathiphu we-pipettezenziwe ngezindinganiso eziphakeme kakhulu futhi zenziwa nge-premium-grade pure polypropylene.
Inkampani yeSuzhou Ace Biomedicalkhiqiza ikhwalithi ephezulu engu-10,20,50,100,200,300,1000 namavolumu angu-1250 µL amathiphu we-pipette wendawo yonke, amathiphu angu-96/irack. Ukuqina okukhethekile - wonke ama-rack amathiphu e-ACE ahlangabezana nezidingo zokusetshenziswa nama-pipettor amaningi. Ayinyumba, Isihlungi, i-RNase-/DNase-free, kanye ne-nonpyrogenic.
Siyakwamukela ukuzosibuza ukuze uthole imininingwane eyengeziwe.
Isikhathi sokuthumela: Dec-29-2022