Amathiphu, njengokusebenzisekayo asetshenziswa nama-pipette, ngokuvamile angahlukaniswa abe: ①. Amathiphu okuhlunga , ②. Amathiphu ajwayelekile, ③. Amathiphu okukhangisa aphansi, ④. Awukho umthombo wokushisa, njll.
1. Ithiphu yesihlungi iyinto esebenzisekayo edizayinelwe ukugwema ukungcoliswa okuphambene. Ivame ukusetshenziswa ekuhlolweni okufana ne-molecular biology, cytology, kanye ne-virology.
2. Ithiphu elijwayelekile liyithiphu elisetshenziswa kakhulu. Cishe yonke imisebenzi yamapayipi ingasebenzisa ithiphu evamile, okuwuhlobo olunomnotho kakhulu lwethiphu.
3. Ngokuhlolwa okunezidingo zokuzwela okuphezulu, noma amasampula ayigugu noma ama-reagents okulula ukuwagcina, ungakhetha ithiphu le-adsorption ephansi ukuze ukhuphule izinga lokutakula. Ingaphezulu lethiphu ye-low-adsorption yenzelwe ukwelashwa kwe-hydrophobic, okunganciphisa uketshezi olucindezelayo olungaphezulu okushiya izinsalela eziningi kuphoyinti. (Isithombe asiphelele futhi inkumbulo inomkhawulo)
PS: Ithiphu yomlomo obanzi ilungele ukumunca izinto ezibonakalayo, i-genomic DNA, kanye noketshezi lwamaseli;
Izinkomba zokusebenza zethiphu: i-adsorption ephansi, isici sokuhlunga, ukuqina, amandla okulayisha nokukhipha, ayikho i-DNase ne-RNase, ayikho i-pyrogen;
Indlela yokukhetha iseluleko esihle? “Inqobo nje uma ithiphu elingafakwa liyithiphu elingasetshenziswa”
——Lokhu ukuqonda okujwayelekile cishe kwabo bonke abasebenzisi mayelana nokuvumelana nezimo kwekhanda lokumunca. Lesi sitatimende singashiwo siyiqiniso ngokwengxenye kodwa singelona iqiniso ngokuphelele.
Iphuzu elingafakwa ku-pipette lingakwazi ngempela ukwakha uhlelo lwe-pipetting nge-pipette ukuze liqaphele umsebenzi wepayipi, kodwa ingabe lokhu kunokwethenjelwa? Kudingeka uphawu lombuzo lapha. Ukuphendula lo mbuzo kudinga idatha ukuze ukhulume.
1. Ungase ufise ukwenza ukuhlolwa kokusebenza ngemva kokufanisa i-pipette nethiphu. Ngemva kokugeza ithiphu, yenza imisebenzi eminingi yokwengeza isampula ephindaphindiwe, kala inani lesampula lesengezo isikhathi ngasinye, bese urekhoda okufundiwe.
2. Bala ukunemba nokunemba kokusebenza kwamapayipi ngemva kokuwuguqula ube yivolumu ngokuvumelana nobuningi boketshezi lokuhlola.
3. Okumele sikukhethe yithiphu enembayo. Uma ukunemba kwe-pipette ne-tip akukuhle, kusho ukuthi ukuqina kwephuzu kanye ne-pipette akukwazi ukuqinisekiswa, ukuze imiphumela yomsebenzi ngamunye ayikwazi ukukhiqizwa kabusha.
Ngakho-ke yimaphi amaphuzu amancane wethiphu elihle?
Ithiphu enhle incike ekugxileni, ekucindezelweni, futhi iphuzu elibaluleke kakhulu i-adsorption;
1. Ake sikhulume nge-taper kuqala: uma kungcono, ukufana nesibhamu kuyoba kuhle kakhulu, futhi ukumuncwa kwe-liquid kuzoba okunembile kakhulu;
2. I-Concentricity: I-concentricity iwukuthi umbuthano phakathi kwesihloko sethiphu kanye nesixhumanisi phakathi kwephuzu ne-pipette yisikhungo esifanayo. Uma kungesona isikhungo esifanayo, kusho ukuthi ukugxila akukuhle;
3. Okokugcina, okubaluleke kakhulu i-adsorptivity yethu: i-adsorptivity ihlobene nempahla yethiphu. Uma impahla yethiphu ingalungile, izothinta ukunemba kwepayipi, okuholela ekugcinweni kwe-liquid enkulu noma isifinyezo Ukulenga odongeni, okubangela amaphutha ku-pipetting;
Ngakho wonke umuntu kufanele anake ngokukhethekile amaphuzu amathathu angenhla lapho ekhetha ikhanda lokumunca
Isikhathi sokuthumela: Oct-30-2021