Ukuhlukaniswa kwamathiphu we-pipette welabhorethri

Ukuhlukaniswa kwamathiphu we-pipette welabhorethri

zingahlukaniswa zibe izinhlobo ezilandelayo: Amathiphu ajwayelekile, amathiphu okuhlunga, amathiphu aphansi we-aspiration, amathiphu ezindawo zokusebenza ezizenzakalelayo kanye namathiphu omlomo obanzi.Ithiphu iklanyelwe ngokukhethekile ukunciphisa ukukhangisa kwesampula kwesampula ngesikhathi senqubo yepayipi. Kuyinto i-laboratory esetshenziswayo engasetshenziswa ngokuhambisana ne-pipette. Isetshenziswa kakhulu ezimeni ezahlukahlukene zamapayipi.

1.I-Universal Pipette Amathiphu

I-Universal Pipette Tips amathiphu asetshenziswa kakhulu, angasetshenziswa cishe kuyo yonke imisebenzi yamapayipi, futhi aphinde abe uhlobo olunomnotho kakhulu lwamathiphu. Ngokuvamile, amathiphu ajwayelekile angafaka imisebenzi eminingi yamapayipi. ezinye izinhlobo zezeluleko nazo ziye zavela kumathiphu ajwayelekile. Ngokuvamile kunezinhlobo eziningi zokupakisha zamathiphu ajwayelekile, futhi kunezinhlobo ezintathu ezivamile emakethe: ezikhwameni, emabhokisini, nasezingcwecweni ezifakwe ngaphambili (ezistakiwe).
Uma abasebenzisi beyisebenzisa, uma benezidingo ezikhethekile zokuvala inzalo, bangathenga amabhokisi afakwe inzalo ngokuqondile. , noma beka amathiphu esikhwama esingahluziwe ebhokisini lethiphu elingenalutho ukuze uzibulale inzalo ngaphambi kokusetshenziswa.

2.Amathiphu Ahlungiwe

Amathiphu ahlungiwe ayinto esebenzisekayo edizayinelwe ukuvikela ukuthelelana ngezifo. Isampula esithathwe ithiphu lesihlungi asikwazi ukungena ngaphakathi kwe-pipette, ngakho izingxenye ze-pipette zivikelwe ekungcoleni nasekugxumeni. Okubaluleke nakakhulu, ingaqinisekisa futhi ukuthi akukho ukungcoliswa kwesiphambano phakathi kwamasampuli futhi isetshenziswa kakhulu ekuhloleni okufana nebhayoloji yamangqamuzana, i-cytology kanye namagciwane.

3.Amathiphu okugcinwa okuphansi kwe-Pipette

Ocwaningweni oludinga ukuzwela okuphezulu, noma amasampula abalulekile noma ama-reagents athambekele ezinsaleni, ungakhetha amathiphu okukhangisa aphansi ukuze uthuthukise ukutakula. Kunezimo lapho kusele okuningi. Kungakhathaliseki ukuthi ukhetha luphi uhlobo lwethiphu, izinga lensalela eliphansi libalulekile.

Uma sibheka ngokucophelela inqubo yokusetshenziswa kwephuzu, sizothola ukuthi lapho uketshezi lukhishwa, kukhona njalo ingxenye engakwazi ukukhishwa futhi ihlale ithiphu. Lokhu kwethula iphutha elithile emiphumeleni kungakhathaliseki ukuthi yikuphi ukuhlola okwenziwayo. Uma leli phutha lingaphakathi kwebanga elamukelekayo, usengakhetha ukusebenzisa ukwaziswa okuvamile.Uma sibheka ngokucophelela inqubo yokusebenzisa ithiphu, sizothola ukuthi lapho uketshezi lukhishwa, kuhlale kunengxenye engakwazi ukukhanywa futhi ihlale. ku tip. Lokhu kwethula iphutha elithile emiphumeleni kungakhathaliseki ukuthi yikuphi ukuhlola okwenziwayo. Uma leli phutha lingaphakathi kwebanga elamukelekayo, usengakhetha ukusebenzisa imiyalo evamile.

4.Amathiphu we-Robotic Pipette

Indawo yokusebenza yethiphu ifaniswa kakhulu nendawo yokusebenzela ewuketshezi, ekwazi ukubona izinga loketshezi futhi iqinisekise ukunemba kwepayipi. Ama-pipette aphuma phambili avame ukusetshenziswa ku-genomics, i-proteomics, i-cytomics, i-immunoassay, i-metabolomics, ucwaningo nokuthuthukiswa kwe-biopharmaceutical, njll. Imikhiqizo yesikhungo sokusebenzela edumile evela kwamanye amazwe ihlanganisa i-Tecan, i-Hamilton, i-Beckman, i-Platinum Elmer (PE) ne-Agilent. Izikhungo zokusebenza zalezi zinhlobo ezinhlanu cishe sezilawule yonke imboni.

5. Amathiphu e-pipette yomlomo obanzi

Izeluleko zomlomo obanzi zilungele i-pipetting viscous materials, i-genomic DNA, kanyeIsiko lamaseliuketshezi; ziyahluka kumathiphu avamile ngokuba nembobo enkulu ezansi ukuze kube lula ukunciphisa amandla kanye nezindlela ezincane. ukusika. Lapho kufakwe amapayipi ama-viscous, ikhanda lokudonsa lendabuko linembobo encane phansi, okungelula ukulithatha nokuconsa, liphinde libangele izinsalela eziphezulu. Idizayini evuthayo isiza ukuphatha amasampula anjalo.

Ebusweni be-DNA ye-genomic kanye namasampula eseli abuthakathaka, uma ukuvuleka kukuncane kakhulu, kulula ukulimaza isampula futhi kubangele ukuphuka kweseli ngesikhathi sokusebenza. Amathiphu ecilongo anokuvulwa okukhudlwana okungaba ngu-70% kunamathiphu ajwayelekile alungile kumasampula antekenteke ngamapayipi. Isixazululo esihle kakhulu.