Emkhakheni wocwaningo lwesayensi, ikakhulukazi emikhakheni efana ne-biochemistry, i-cell biology, ne-pharmacology, ukukhethwa kwemishini yaselabhorethri kungaba nomthelela omkhulu ekusebenzeni kahle nokunemba kokuhlolwa. Esinye sesinqumo esibaluleke kangaka ukukhetha phakathi kwamapuleti emithombo engama-96 nama-384. Zombili izinhlobo zamapuleti zinamasethi azo ezinzuzo kanye nezithiyo ezingaba khona. Ukhiye wokuthuthukisa ukusebenza kahle kwelebhu usekuqondeni lo mehluko nokukhetha leyo evumelana kangcono nezidingo ezithile zokuhlolwa.

1. Umthamo kanye Nomphumela

Omunye umehluko oyinhloko phakathi kwamapuleti emithombo engu-96 kanye nama-384 inombolo yemithombo, ethinta ngokuqondile umthamo wama-reagents angasetshenziswa kanye nokuphuma kokuhlolwa. Ipuleti elinemithombo engu-96, elinemithombo emikhulu, ngokuvamile libamba umthamo owengeziwe, okulenza lifaneleke izivivinyo ezidinga ama-reagents amaningi noma amasampuli, kanye nokuhlolwa lapho ukuhwamuka kungase kubangele ukukhathazeka. Ngokuphambene, amapuleti anemithombo engu-384, anomthamo omkhulu wemithombo, avumela inani elikhulu lokuhlola ngasikhathi sinye, ngaleyo ndlela andise ukuphuma ngokuphawulekayo. Lokhu kunenzuzo ikakhulukazi ezinhlelweni zokusebenza ze-high-throughput screening (HTS), lapho ikhono lokucubungula izinombolo ezinkulu zamasampuli ngokushesha libalulekile.

2. Ukusebenza Kwezindleko

Izindleko zingesinye isici esibalulekile okufanele sicatshangelwe. Nakuba amapuleti anemithombo engu-384 evame ukuvumela ukuhlolwa okwengeziwe ngepuleti ngalinye, okunganciphisa izindleko zokuhlolwa ngakunye, angase futhi adinge imishini yokuphatha uketshezi enembile futhi evame ukubiza kakhulu. Ukwengeza, amavolumu amancane e-reagent asetshenziswa kumapuleti anemithombo engu-384 angaholela ekongeni kwezindleko ezibalulekile kuma-reagents ngokuhamba kwesikhathi. Kodwa-ke, amalebhu kufanele alinganise lokhu konga nokutshalwa kwezimali kokuqala kumishini ethuthuke kakhulu.

3. Ukuzwela kanye Nekhwalithi Yedatha

Ukuzwela kokuhlolwa okwenziwe kumapuleti wemithombo engu-96 uma kuqhathaniswa namapuleti angama-384 nakho kungahluka. Ngokuvamile, ivolumu enkulu kumapuleti anemithombo engu-96 ingasiza ekunciphiseni ukuhlukahluka futhi ithuthukise ukukhiqizwa kabusha kwemiphumela. Lokhu kubenza bafaneleke ukuhlolwa lapho ukunemba kubaluleke kakhulu. Ngakolunye uhlangothi, amapuleti emithombo engu-384, anevolumu emincane, angandisa ukuzwela ekuhloleni okuthile, njenge-fluorescence noma ama-assays asekelwe ku-luminescence, ngenxa yokugxila okuphezulu kwesignali.

4. Ukusetshenziswa Kwendawo

Indawo yaselabhorethri imvamisa iba sezingeni eliphezulu, futhi ukukhetha kwepuleti kungaba nomthelela ekutheni lesi sikhala sisetshenziswa kahle kangakanani. Amapuleti anemithombo engu-384 enza ukuhlolwa okwengeziwe kwenziwe endaweni efanayo uma kuqhathaniswa namapuleti anemithombo engama-96, okwenza kube ngcono ibhentshi lelebhu kanye nesikhala se-incubator. Lokhu kungaba yinzuzo ikakhulukazi kumalebhu anesikhala esilinganiselwe noma lapho imisebenzi ephezulu ibalulekile.

5. Ukuhambisana Kwezisetshenziswa

Ukuhambisana nemishini yelebhu ekhona kungenye indlela ebalulekile yokucatshangelwa. Amalabhorethri amaningi asenayo imishini eklanyelwe amapuleti anemithombo engu-96, kusukela kumarobhothi afaka amapayipi kuya kubafundi bamapuleti. Ukushintshela kumapuleti anemithombo engu-384 kungase kudinge okokusebenza okusha noma ukuguqulwa kwezinhlelo ezikhona, ezingabiza futhi zidle isikhathi. Ngakho-ke, amalebhu kufanele ahlole ngokucophelela ukuthi ingabe izinzuzo zokushintshela kumapuleti anemithombo engama-384 ziyazidlula lezi zinselele ezingaba khona.

Isiphetho

Ekugcineni, isinqumo phakathi kokusebenzisa amapuleti emithombo engama-96 noma angu-384 sincike ezidingweni ezithile zaselabhorethri kanye nohlobo lokuhlolwa okwenziwayo. Ocwaningweni oludinga amavolumu amakhulu nalapho ukuzwela nokukhiqiza kubalulekile, amapuleti emithombo engu-96 angaba yinketho engcono. Ngokuphambene, ekusetshenzisweni komkhiqizo ophezulu kanye nokusebenza kahle kwezindleko mayelana nokusetshenziswa kwe-reagent, amapuleti emithombo engama-384 angathuthukisa kakhulu ukusebenza kahle kwelabhorethri. Amalabhorethri kufanele acubungule lezi zici ngokucophelela, ecabangela izimo zazo eziyingqayizivele, ukuze enze ukukhetha okunolwazi kakhulu nokuphumelelayo.