Endaweni yocwaningo lwesayensi, ikakhulukazi emikhakheni efana ne-biochemistry, i-spel biology, kanye ne-pharmacology, ukukhethwa kwemishini yelebhu kungathinta kakhulu ukusebenza kahle nokunemba kokuhlolwa. Isinqumo esisodwa esinjalo ukukhethwa phakathi kwamapuleti aphakathi kwama-96-kahle nama-384-kahle. Zombili izinhlobo zepuleti zinamasethi wazo ezinzuzo nezingqinamba ezingaba khona. Isihluthulelo sokwenza kahle ukusebenza kahle kwelebhu silele ekuqondeni lokhu kwehluka futhi ukukhetha okulungele kangcono izidingo ezithile zokuhlolwa.

1. Ivolumu nokudlula

Enye yamahluko okuyisisekelo phakathi kwama-96-kahle nama-384-kahle amapuleti yinani lemithombo, elithinta ngqo umthamo we-reagents ongasetshenziswa kanye nokugcwaliswa kwezivivinyo. Ipuleti eli-96-kahle, elinemithombo emikhulu, imvamisa liphethe ivolumu ethe xaxa, okwenza lilungele ama-akhawuntana adinga ama-reagents noma amasampula amaningi, kanye nokuhlolwa lapho ukuhwamuka kungakhathazeka khona. Ngakolunye uhlangothi, amapuleti ama-384-kahle, ngokuqina kwawo okuphezulu kwemithombo, avumele inani elikhulu lemibono ngasikhathi sinye, okwenza kwanda ukukhishwa okuphawuleka kakhulu. Lokhu kuzuzisa kakhulu ukusetshenziswa kokuhlola okuphezulu (HTS), lapho amandla okucubungula amanani amakhulu amasampula ngokushesha abucayi.

2. Ukusebenza kwezindleko

Izindleko kungenye into ebucayi okufanele uyicabangele. Ngenkathi amapuleti angu-384-kahle avame ukuvumela imihlandlela eyengeziwe ngepuleti ngalinye, elinganciphisa izindleko nge-assay ngayinye, zingadinga nemishini yokuphatha ethe xaxa futhi evame ukubiza kakhulu. Ngaphezu kwalokho, amavolumu amancane we-realent asetshenziswa kumapuleti angama-384-kahle angaholela ekugcinweni kwezindleko ezibalulekile kuma-reagonts ngokuhamba kwesikhathi. Kodwa-ke, ama-labs kumele alinganise lokhu okulondoloziwe ngokutshalwa kwemali kokuqala emishini esezingeni eliphakeme.

3. Ukuzwela nekhwalithi yedatha

Ukuzwela kwezindlela ezenziwa ku-98-kahle amapuleti angama-384-kahle nakho kungahluka. Ngokuvamile, umqulu omkhulu kumapuleti angama-96-kahle angasiza ukunciphisa ukuhlukahluka futhi athuthukise kabusha ukuze kube nemiphumela. Lokhu kubenza balungele izivivinyo lapho ukunemba kubaluleke kakhulu. Ngakolunye uhlangothi, amapuleti ama-384-kahle, ngamavolumu amancane, angakhulisa ukuzwela ezimweni ezithile, njenge-fluorescence noma agamesi asuselwa ku-luminescence, ngenxa yokuqothuka okuphezulu kwesiginali.

4. Ukusetshenziswa kwesikhala

Isikhala selebhu sivame kwiprimiyamu, futhi ukukhetha kwepuleti kungathinta ukuthi lesi sikhala sisetshenziswe kahle kangakanani. Amapuleti angama-384-kahle anika amandla okuthe xaxa Lokhu kungazuzisa kakhulu ama-labs anesikhala esilinganiselwe noma lapho kubalulekile khona imisebenzi ephezulu kakhulu.

5. Ukuvumelana Kwemishini

Ukuhambisana kwemishini yelebhu ekhona kungenye yezinto ezibalulekile. Ama-laboratories amaningi asevele anemishini esetshenziselwa amapuleti angu-96 kahle, kusuka kumarobhothi e-Pipetting ukuya kubafundi bePlate. Ukushintshela kumapuleti angama-384-kahle kungadinga imishini emisha noma ukuguqulwa ezinhlelweni ezikhona, ezingabiza kakhulu nangesikhathi. Ngakho-ke, ama-labs kumele ahlole ngokucophelela ukuthi izinzuzo zokushintshela kumapuleti angu-384-kahle zidlula lezi zinselelo ezingaba khona.

Ukugcina

Ekugcineni, isinqumo phakathi kokusebenzisa amapuleti angu-96-kanye noma ama-384-kahle amapuleti ezidingweni ezithile zelebhu kanye nohlobo lwezivivinyo ezenziwayo. Okokuhlola okudinga amavolumu amakhulu nalapho ukuzwela nokuzala kubalulekile, amapuleti ane-96 kahle kungaba ukukhetha okungcono. Ngakolunye uhlangothi, ngezicelo zokusebenzisa phezulu kanye nokusebenza kwezindleko ngokuya ngokusetshenziswa kwe-realent, amapuleti ama-384 akwazi ukuthuthukisa kakhulu ukusebenza kwelabhoratri. Ama-laboraries kumele acashile lezi zinto ngokucophelela, ecubungula izimo zawo ezihlukile, ukwenza ukukhetha okunolwazi kakhulu futhi okusebenzayo.