Amapuleti e-PCR ngokuvamile asebenzisa amafomethi we-96-well kanye nama-384-well, alandelwa yi-24-well kanye ne-48-well. Imvelo yomshini we-PCR osetshenzisiwe kanye nohlelo lokusebenza oluqhubekayo luzonquma ukuthi ipuleti le-PCR likulungele yini ukuhlola kwakho.
Isiketi
"Isiketi" sepuleti le-PCR yipuleti elizungeze ipuleti. Isiketi singanikeza ukuzinza okungcono kwenqubo ye-pipetting ngesikhathi sokwakhiwa kwesistimu yokusabela, futhi inikeze amandla angcono wemishini ngesikhathi sokucubungula okuzenzakalelayo kwemishini. Amapuleti e-PCR angahlukaniswa abe kungabikho iziketi, iziketi eziyingxenye kanye neziketi ezigcwele.
Indawo yebhodi
Ingaphezulu lebhodi libhekisela endaweni yalo engaphezulu.
Idizayini yephaneli eyisicaba egcwele ifanele imishini eminingi ye-PCR futhi kulula ukuyivala nokuyiphatha.
Umklamo wepuleti onqenqemeni ophakeme unokuvumelana nezimo ezingcono kakhulu kumathuluzi athile e-PCR, okusiza ukulinganisa ukucindezela kwekhava yokushisa ngaphandle kwesidingo sama-adaptha, ukuqinisekisa ukudluliswa kokushisa okungcono kakhulu kanye nomphumela wokuhlola okuthembekile.
Umbala
Amapuleti e-PCRngokuvamile atholakala ngamafomethi ahlukahlukene emibala ukuze kube lula ukuhlukaniswa okubonakalayo nokuhlonza amasampuli, ikakhulukazi ekuhlolweni komphumela ophezulu. Yize umbala wepulasitiki ungenawo umthelela ekukhuliseni i-DNA, lapho usetha ukusabela kwe-PCR yesikhathi sangempela, sincoma ukuthi kusetshenziswe izinto ezidlekayo zepulasitiki ezimhlophe noma okokusetshenziswa kwepulasitiki okuyisithwathwa ukuze kuzuzwe i-fluorescence ezwelayo nenembile uma iqhathaniswa nezinto ezisetshenziswayo ezibonakalayo. Izinto ezisetshenziswayo ezimhlophe zithuthukisa ukuzwela nokuvumelana kwedatha ye-qPCR ngokuvimbela i-fluorescence ekuphumeni ngaphandle kweshubhu. Uma i-refraction incishisiwe, isignali eyengeziwe iboniswa emuva kumtshina, ngaleyo ndlela ikhulise isilinganiso sesignali-kumsindo. Ngaphezu kwalokho, udonga lwamashubhu amhlophe luvimbela isignali ye-fluorescent ukuthi idluliselwe kumojula yensimbi ye-PCR, igwema ukumuncwa noma ukukhombisa ngokungahambisani isignali ye-fluorescent, ngaleyo ndlela kuncishiswe umehluko ekuhlolweni okuphindaphindiwe.
Izinhlobo ezahlukene zezinsimbi, ngenxa yomklamo ohlukile wendawo yomtshina we-fluorescence, sicela ubhekisele ku-manuf.
Isikhathi sokuthumela: Nov-13-2021