Amasistimu okuphatha uketshezi oluzenzakalelayo anezinzuzo eziningi lapho ephatha uketshezi oluyinkinga njengoketshezi olubonakalayo noma oluguquguqukayo, kanye namavolumu amancane kakhulu. Amasistimu anamasu okuletha imiphumela enembile nethembekile ngamanye amaqhinga ahlelekayo kusofthiwe.
Ekuqaleni, isistimu yokuphatha uketshezi oluzenzakalelayo ingase ibonakale iyinkimbinkimbi futhi ingaphezu kwamandla. Kodwa uma usuqalile ukusebenza ngala madivayisi, uzobona ukuthi akwenza kube lula kanjani ukuhamba kwakho komsebenzi. Onjiniyela bathuthukise izici eziningi ezihlukene ukuze kube lula izinhlelo zokusebenza eziyinselele.
Lapho uphatha amavolumu amancane ngezinhlelo zokubamba uketshezi ezizenzakalelayo, kungenzeka ukuthi ufise wonke ama-reagents adingekayo ekuphenduleni kokukodwa.ithiphu, ehlukaniswe igebe lomoya. Le nqubo kuxoxwa ngayo kabanzi, ikakhulukazi mayelana nokungcoliswa koketshezi oluhlukene ngamaconsi angaphandleithiphu ye-pipette. Abanye abakhiqizi batusa lokhu noma kunjalo ukuze wonge isikhathi nomzamo. Amasistimu angakwazi ukulangazelela amanzi kuqala, kulandele i-reagent A, bese kuba i-reagent B, njll. Ungqimba ngalunye lwe-liquid luhlukaniswa negebe lomoya ukuvimbela ukuxubana noma ukusabela kuqale ngaphakathi kwethiphu. Lapho uketshezi lukhishwa, wonke ama-reagents axutshwa ngokuqondile futhi amanani amancane kakhulu ahlanzwa ngaphandleithiphungamavolumu amakhulu esihlokweni. Ithiphu kufanele ishintshwe ngemva kwesinyathelo ngasinye se-pipetting.
Inketho engcono ukusebenzisa amathuluzi akhethekile alungiselelwe amavolumu amancane, isb, ukudlulisa amavolumu angu-1 µL ekunikezeni i-free-jet. Lokhu kwandisa isivinini futhi kugwema ukungcoliswa okuphambene. Uma amavolumu angaphansi kuka-1 µl efakwe ngamapayipi, kungcono ukuwakhipha ngokuqondile oketshezini oluqondiwe noma ngokumelene nendawo yomkhumbi ukuze ukhiphe yonke ivolumu. Ukukhipha amavolumu amancane ngokuthinta uketshezi nakho kuyatuswa lapho uketshezi oluyinselelo olufana noketshezi lwe-viscous lufakwa ngamapayipi.
Esinye isici esiwusizo kakhulu sezinhlelo zokubamba uketshezi ezizenzakalelayo ukucwiliswa kwethiphu. Lapho kuphela isampula engu-1 µL ifunwa ku-ithiphu, i-liquid drop ngokuvamile inamathela ngaphandleithiphungesikhathi sokukhulelwa. Kungenzeka ukuhlela ithiphu ukuthi icwilise oketshezini emthonjeni ukuze amaconsi namaconsi amancane angaphandle kwethiphu afinyelele ukusabela.
Ngaphezu kwalokho, ukusetha i-aspiration nesivinini sokukhipha kanye nevolumu yokuphuma nesivinini kuyasiza futhi. Isivinini esiphelele sohlobo ngalunye loketshezi nevolumu singahlelwa. Futhi ukusetha le mingcele kuholela emiphumeleni ekwazi ukukhiqizwa kabusha kakhulu ngoba sifaka i-pipette ngesivinini esihlukile nsuku zonke kuye ngokusebenza kwethu siqu. Ukuphatha uketshezi okuzenzakalelayo kungadambisa ingqondo yakho futhi kwandise ukwethemba izinhlelo zokusebenza eziyinselele ngokuthatha izingxenye ezicasulayo.
Isikhathi sokuthumela: Feb-07-2023