Ochwepheshe baselabhorethri bangachitha amahora usuku ngalunye bephethe i-micropipette, futhi ukuthuthukisa ukusebenza kahle kwamapayipi kanye nokuqinisekisa imiphumela enokwethenjelwa ngokuvamile kuyinselele.Ukukhetha i-micropipette efanele yanoma yisiphi isicelo kuyisihluthulelo sempumelelo yomsebenzi waselabhorethri; akuqinisekisi kuphela ukusebenza kwanoma yikuphi ukuhlola, kodwa futhi kwandisa ukusebenza kahle.Ukuqonda izidingo zokuhamba komsebenzi wamapayipi kwenza abasebenzisi bakwazi ukukhetha amapayipi anembile futhi aphindaphindwayo, kodwa kunezinye izici eziningi okufanele zicatshangelwe ukuze kuthuthukiswe imiphumela yepayipi nokuqinisekisa impumelelo yocwaningo.
Uma sikhuluma kabanzi, uketshezi luwela ezigabeni ezintathu eziyinhloko: okunamanzi, okune-viscous, nokuguquguqukayo.Uketshezi oluningi lusekelwe emanzini, okwenza amapayipi okuthutha umoya abe yisinqumo sokuqala kwabaningi.Nakuba uketshezi oluningi lusebenza kahle nalolu hlobo lwe-pipette, amapayipi e-volumetric kufanele akhethwe lapho kusebenza noketshezi olubonakala kakhulu noma oluguquguqukayo.Umehluko phakathi kwalezi zinhlobo zamapayipi oketshezi ukhonjiswa futhi ku-Figure owuketshezi okufanele usetshenziswe ku-Figure. thayipha - ukuthola imiphumela emihle kakhulu.
Amapharamitha amabili abaluleke kakhulu athinta imiphumela yamapayipi ukunemba nokunemba (Umfanekiso 2). Ukuze kuzuzwe ukunemba okuphezulu kwamapayipi, ukunemba, nokwethembeka, imibandela embalwa kufanele ikhunjulwe.Njengomthetho wesithupha, umsebenzisi kufanele ahlale ekhetha ipayipi elincane elingakwazi ukuphatha ivolumu yokudlulisa oyifunayo.Lokhu kubalulekile njengoba ukunemba kuncipha njengoba isethi yevolumu yepayipi isondela ku-50. µl nge-pipette engu-5,000 µl, imiphumela ingase ibe mibi. Imiphumela engcono ingatholwa ngamapayipi angu-300 µl, kuyilapho ama-pipette angu-50 µl enikeza imiphumela engcono kakhulu. Ngaphezu kwalokho, ivolumu elisethwe kumapayipi enziwe ngesandla angashintsha ngesikhathi sokubhoboza amapayipi ngenxa yokuzungezisa ngephutha kwephayiphi. ukuze uqhubeke uqinisekisa ukunemba.Ukulinganisa kungenye into ebalulekile esiza ukuqinisekisa imiphumela enokwethenjelwa ngokubonisa ukunemba nokunemba kwe-pipette.Le nqubo kufanele ibe lula kumsebenzisi; isibonelo, amanye amapayipi e-elekthronikhi angasetha izikhumbuzi zokulinganisa, noma alondoloze umlando wokulinganisa.Akuwona nje amapayipi okufanele acatshangelwe.Uma ithiphu le-pipette lixega, livuza, noma liwa, lingabangela izinkinga ezihlukahlukene.Le nkinga evamile elebhu ivame ukubangelwa ukusetshenziswa kwamathiphu epayipi enhloso evamile, okuvame ukudinga "ukuthepha."Le nqubo ingabangela ukuthi i-tip yeluleke noma ihlehle i-tip. indawo engafanele, noma kubangele nokuthi ithiphu liwe ngokuphelele ku-pipette.Ukukhetha i-micropipette yekhwalithi ephezulu eklanywe ngamathiphu athile kuqinisekisa ukuxhumana okuphephile, okuhlinzeka ngezinga eliphezulu lokuthembeka kanye nemiphumela engcono.Ukwengeza, into elula njengama-pipette afaka amakhodi umbala futhi angasiza abasebenzisi ukuqinisekisa ukuthi amathiphu afanele akhethelwa amapayipi abo.
Esimweni esikhiqiza kakhulu, kubalulekile ukusebenza kahle ngangokunokwenzeka kuyilapho kugcinwa ukwethembeka nokuvumelana kwenqubo yokufaka amapayipi.Kunezindlela eziningi zokuthuthukisa ukusebenza kahle kwamapayipi, okuhlanganisa ukusetshenziswa kwamapayipi amaningi kanye/noma amapayipi kagesi.Lawa amathuluzi ahlukahlukene avame ukunikeza izindlela eziningana zokubhoboza amapayipi—njengokuhlanekezela amapayipi, ukusabalalisa okuguquguqukayo, ukwenza lula inqubo, ukwenza lula inqubo, ukwenza lula inqubo, ukwenza lula inqubo. njengokukhipha okuphindaphindiwe kulungele ukunikeza ama-aliquots amaningi evolumu efanayo ngaphandle kokugcwalisa ithiphu.Ukusebenzisa amapayipi esiteshi esisodwa ukudlulisa amasampula phakathi kwamafomethi ahlukene e-labware kungase kusheshe kube yisicefe futhi kube namaphutha.Amapayipi amaningi amashaneli avumela ukudlulisa amasampula amaningi ngesikhathi esisodwa ngokucwayiza kweso.Akukhona nje ukuthi lokhu kukhulisa ukusebenza kahle kwamapayipi, kusiza futhi ukuvimbela ukulimala kwamapayipi, futhi kuyasiza futhi ukuqeqesha iRS). ama-pipette anekhono lokushintsha izikhala zethiphu ngesikhathi sokufaka amapayipi, okuvumela ukudluliswa okufanayo kwamasampula amaningi phakathi kosayizi namafomethi we-labware ehlukene, okonga amahora esikhathi (Umfanekiso 3).
Ochwepheshe baselabhorethri ngokuvamile bachitha amahora ngosuku beshaya ngamapayipi. Lokhu kungabangela ukungaphatheki kahle futhi, ezimweni ezimbi kakhulu, ngisho nokulimala kwesandla noma kwengalo. Iseluleko esingcono kakhulu sokugwema lezi zingozi ezingase zibe khona ukunciphisa isikhathi obambe ngaso i-pipette sibe isikhathi esifushane ngangokunokwenzeka. Ngaphezu kwalokhu, abasebenzisi kufanele bakhethe i-micropipette engasindi nebhalansiswe kahle enesisindo phakathi nendawo ukuze izinze kangcono, abasebenzisi esandleni sokudla kufanele babe nesandla sokudla esinethezekile. idizayini yokubamba, futhi ulungise ivolumu ngokunethezeka nangokushesha ngangokunokwenzeka ukuze ugweme ukunyakaza okungadingekile.Futhi, amathiphu abalulekile, njengoba ukulayishwa kwethiphu kanye nokukhipha ngokuvamile kudinga amandla angaphezu kwepayipi futhi kunengozi engaba khona yokulimala, ikakhulukazi kuzilungiselelo eziphezulu.
Lapho ukhetha i-micropipette efanele yohlelo lwakho lokusebenza, kubalulekile ukubheka zonke izici zokuhamba komsebenzi wakho.Ngokucabangela i-pipette, izici zayo, uhlobo nomthamo woketshezi olufakwa ngamapayipi, namathiphu asetshenziswayo, ososayensi bangaqinisekisa imiphumela enembile, enembile nethembekile kuyilapho begcina ukukhiqiza futhi benciphisa ingozi yokulimala.
Kulolu shicilelo, ukutholwa kokuhlaziya okuyisisekelo kuhlolwa yi-HPLC-MS kusetshenziswa ama-microplates e-SPE wemodi exubile ye-cation exchange. Izinzuzo ze-SEC-MALLS kuzicelo ze-biopharmaceutical…
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Isikhathi sokuthumela: Jun-10-2022