Ochwepheshe baselabhorethri bangachitha amahora usuku ngalunye bephethe i-micropipette, futhi ukuthuthukisa ukusebenza kahle kwamapayipi kanye nokuqinisekisa imiphumela enokwethenjelwa ngokuvamile kuyinselele.Ukukhetha i-micropipette efanele yanoma yisiphi isicelo kuyisihluthulelo sempumelelo yomsebenzi waselabhorethri; akuqinisekisi ukusebenza kwanoma yikuphi ukuhlola kuphela, kodwa futhi kwandisa ukusebenza kahle.Ukuqonda izidingo zokugeleza kwamapayipi kwenza abasebenzisi bakwazi ukukhetha amapayipi anembile naphindaphindwayo, kodwa kunezici eziningi okufanele zicatshangelwe ukuze kuthuthukiswe imiphumela yepayipi kanye nokuqinisekisa impumelelo yamapayipi. ukuhlola.
Ngobubanzi, uketshezi luwela ezigabeni ezintathu eziyinhloko: ezinamanzi, i-viscous, kanye ne-volatile.Iziphuzo eziningi zisekelwe emanzini, okwenza amapayipi okuhambisa umoya abe yisinqumo sokuqala kwabaningi.Nakuba uketshezi oluningi lusebenza kahle nalolu hlobo lwe-pipette, amapayipi e-volumetric kufanele akhethwe uma ukusebenza nge-viscous noma uketshezi oluguquguqukayo kakhulu.Umehluko phakathi kwalezi zinhlobo zamapayipi uboniswa kuMfanekiso 1.Kubalulekile futhi ukusebenzisa indlela efanele yokufaka amapayipi - kungakhathaliseki ukuthi uketshezi luni. thayipha - ukuthola imiphumela emihle kakhulu.
Imingcele emibili ebaluleke kakhulu ethinta imiphumela ye-pipetting ukunemba nokunemba (Umfanekiso 2). Ukuze kuzuzwe ukunemba okuphezulu kwamapayipi, ukunemba, nokuthembeka, imibandela ethile kufanele ikhunjulwe.Njengomthetho wesithupha, umsebenzisi kufanele ahlale ekhetha i-piette encane kunazo zonke. engakwazi ukuphatha ivolumu yokudlulisa oyifunayo.Lokhu kubalulekile njengoba ukunemba kuncipha njengoba ivolumu ebekiwe isondela kumthamo omncane we-pipette.Isibonelo, uma ukhipha u-50 I-µl nge-5,000 µl pipette, imiphumela ingase ibe mibi. Imiphumela engcono ingatholwa ngamapayipi angu-300 µl, kuyilapho ama-pipette angu-50 µl ehlinzeka ngemiphumela engcono kakhulu. I-plunger.Kungakho abanye abakhiqizi be-pipette baye bahlakulela imiklamo yokuvala ivolumu yokuvala ukuvimbela izinguquko ezingalindelekile ngenkathi i-pipettting. ukuze uqhubeke uqinisekisa ukunemba.Ukulinganisa kungenye into ebalulekile esiza ukuqinisekisa imiphumela enokwethenjelwa ngokubonisa ukunemba nokunemba kwe-pipette.Le nqubo kufanele ibe lula kumsebenzisi; isibonelo, amanye amapayipi e-elekthronikhi angasetha izikhumbuzi zokulinganisa, noma alondoloze umlando wokulinganisa.Akuwona nje amapayipi okufanele acatshangelwe.Uma ithiphu le-pipette lixega, livuze, noma liwe, lingabangela izinkinga ezihlukahlukene.Le nkinga evamile elebhu kuvame ukubangelwa ukusetshenziswa kwamathiphu e-pipette enhloso evamile, avame ukudinga “ukuthepha.”Le nqubo yelula unqenqema lwethiphu ye-pipette futhi ingabangela ukuthi ithiphu livuze noma endaweni engafanele, noma kubangele ukuthi ithiphu liwe ngokuphelele ku-pipette.Ukukhetha i-micropipette yekhwalithi ephezulu eklanywe ngamathiphu athile kuqinisekisa ukuxhumana okuphephile, okuhlinzeka ngezinga eliphezulu lokuthembeka nemiphumela engcono.Ukwengeza, into elula njengamapayipi anekhodi yombala. futhi amathiphu angasiza abasebenzisi ukuqinisekisa ukuthi amathiphu alungile akhethiwe kuma-pipettes abo.
Esimweni esisezingeni eliphezulu, kubalulekile ukusebenza kahle ngangokunokwenzeka ngenkathi kugcinwa ukwethembeka nokuvumelana kwenqubo yepayipi.Kunezindlela eziningi zokuthuthukisa ukusebenza kahle kwamapayipi, okuhlanganisa ukusetshenziswa kwe-multichannel kanye/noma amapayipi kagesi.Lezi zinsimbi eziguquguqukayo ngokuvamile zinikeza izindlela ezimbalwa ezihlukene zamapayipi—njengamapayipi ahlehlayo, ukusatshalaliswa okuguquguqukayo, ukuhlanjululwa kwe-serial okuhleliwe, nokunye—ukwenza inqubo ibe lula.Isibonelo, izinqubo njengokukhipha okuphindaphindiwe kulungele ukunikeza ama-aliquot amaningi wevolumu efanayo ngaphandle kokugcwalisa ithiphu.Ukusebenzisa amapayipi esiteshi esisodwa ukudlulisa amasampula phakathi kwamafomethi ahlukene e-labware kungaba ngokushesha okuyisicefe futhi kube namaphutha.Amapayipi amashaneli amaningi avumela ukudlulisa amasampula amaningi ngesikhathi esisodwa. ngokucwayiza kweso.Lokhu akugcini nje ukwandisa ukusebenza kahle, futhi kusiza ukuvimbela amaphutha epayipi kanye nokulimala okuphindaphindayo (RSI).Abanye ama-pipette anekhono lokushintsha izikhala zethiphu ngesikhathi sokufaka amapayipi, okuvumela ukudluliswa okufanayo kwamasampula amaningi phakathi kosayizi namafomethi we-labware ehlukene, okonga amahora esikhathi (Umfanekiso 3).
Ochwepheshe baselabhorethri ngokuvamile bachitha amahora ngosuku betting ipayipi.Lokhu kungabangela ukungakhululeki futhi, ezimweni ezimbi kakhulu, ngisho nokulimala kwesandla noma kwengalo.Iseluleko esingcono kakhulu sokugwema lezi zingozi ezingase zibe khona ukunciphisa isikhathi obamba i-pipette esikhathini esifushane kakhulu. .Ngaphezu kwalokhu, abasebenzisi kufanele bakhethe i-micropipette engasindi futhi elinganiseke kahle enesisindo phakathi nendawo ukuze izinze kangcono.I-pipette kufanele ilingane kahle ezandleni zabasebenzisi besandla sobunxele nakwesokudla, ibe nokuhle. idizayini yokubamba, futhi ulungise ivolumu ngokunethezeka nangokushesha ngangokunokwenzeka ukuze ugweme ukunyakaza okungadingekile.Futhi, amathiphu abalulekile, njengoba ukulayisha ithiphu kanye nokukhishwa ngokuvamile kudinga amandla angaphezu kwamapayipi futhi kunengozi engaba khona yokulimala, ikakhulukazi kuzilungiselelo eziphezulu ze-throughput. .Amathiphu we-Pipette kufanele avele endaweni ngamandla amancane, anikeze uxhumano oluvikelekile, futhi kube lula ngokulinganayo ukuwakhipha.
Lapho ukhetha i-micropipette efanele yohlelo lwakho lokusebenza, kubalulekile ukubheka zonke izici zokuhamba komsebenzi wakho.Ngokucabangela i-pipette, izici zayo, uhlobo nomthamo woketshezi olufakwa ngamapayipi, namathiphu asetshenziswayo, ososayensi bangaqinisekisa ukuthi kunembe, kunembe futhi kunokwethenjelwa. imiphumela ngenkathi kugcinwa ukukhiqiza kanye nokunciphisa ingozi yokulimala.
Kulolu shicilelo, ukutholwa kokuhlaziya okuyisisekelo kuhlolwa yi-HPLC-MS kusetshenziswa ama-microplates e-SPE wemodi exubile ye-cation exchange. Izinzuzo ze-SEC-MALLS kuzicelo ze-biopharmaceutical…
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Isikhathi sokuthumela: Jun-10-2022