I-Polymerase chain reaction (PCR) iyindlela esetshenziswa kakhulu kubhayoloji yamangqamuzana ekukhuliseni izingcezu ze-DNA. I-PCR ibandakanya izinyathelo ezimbalwa, okuhlanganisa ukuhunyushwa, ukuhunyushwa, nokunwetshwa. Impumelelo yale nqubo incike kakhulu kwikhwalithi yamapuleti e-PCR namashubhu asetshenzisiwe. Kunezici eziningi okufanele uzicabangele lapho ukhetha amapuleti namashubhu e-PCR afanelekile wohlelo lwakho lokusebenza. Nazi ezinye izici ezibalulekile okufanele uzicabangele:
1. AmandlaAmapuleti e-PCRnamashubhu eza ngobukhulu obuhlukene kanye nekhono. Ukukhethwa kobukhulu namandla kuncike kakhulu enanini le-DNA okudingeka ikhuliswe ngokusabela okukodwa. Isibonelo, uma udinga ukukhulisa inani elincane le-DNA, ungakhetha ishubhu elincane. Uma inani elikhulu le-DNA lidinga ukukhuliswa, ipuleti elinomthamo omkhulu lingakhethwa.
2. Amapuleti we-PCR we-Material namashubhu angenziwa ngezinto ezahlukene ezifana ne-polypropylene, i-polycarbonate noma i-acrylic. I-Polypropylene iyinto esetshenziswa kakhulu ngenxa yokumelana namakhemikhali nokushisa. Ibuye ingabizi kakhulu uma iqhathaniswa nezinye izinto zokwakha. Ama-Polycarbonates nama-acrylics abiza kakhulu, kodwa anokucaca okungcono kokubona futhi alungele i-PCR yesikhathi sangempela.
3. I-PCR ye-thermal conductivity ihlanganisa imijikelezo eminingi yokushisa, edinga ukufudumeza okusheshayo nokupholisa kwengxube yokusabela. Ngakho-ke, amapuleti e-PCR namashubhu kufanele abe ne-conductivity enhle yokushisa ukuze kuqinisekiswe ukushisa okufanayo nokupholisa kwengxube yokusabela. Amapuleti anezindonga ezincane kanye nezindawo eziyisicaba zilungele ukwandisa ukudluliswa kokushisa.
4. Ukuhambisana kwamapuleti namashubhu e-PCR kufanele kuhambisane nomjikelezo oshisayo owusebenzisayo. Amapuleti namashubhu kufanele akwazi ukumelana namazinga okushisa aphezulu adingekayo ukuze kukhuliswe izingcezu ze-DNA. Hlala uthintana nomkhiqizi wamabhayisikili ashisayo ukuze uthole amapuleti namashubhu anconyiwe.
5. Seal Uphawu oluqinile lubalulekile ukuvimbela ukungcoliswa kwengxube yokusabela. Amapuleti e-PCR namashubhu angavalwa kusetshenziswa izindlela ezahlukene ezifana nezimpawu zokushisa, amafilimu anamathelayo noma izivalo. Ukuvalwa kokushisa kuyindlela ephephe kunazo zonke futhi kunikeza umgoqo oqinile ekungcoleni.
6. Ukuvala inzalo amapuleti e-PCR namashubhu kufanele kungabi nakungcola okungase kuphazamise ukusabela. Ngakho-ke, kufanele zifakwe inzalo ngaphambi kokusetshenziswa. Kubalulekile ukukhetha amapuleti namashubhu okulula ukuwavala amagciwane futhi amelana nezindlela zokuvala amakhemikhali nezokushisa.
Kafushane, ukukhetha ipuleti elilungile le-PCR namashubhu kubalulekile ekukhuliseni ngempumelelo i-DNA. Inketho incike kakhulu ohlotsheni lohlelo, inani le-DNA ekhulisiwe, kanye nokuhambisana namabhayisikili ashisayo.
I-Suzhou Ace Biomedical Technology Co., Ltd. inikeza izinhlobonhlobo zamapuleti namashubhu e-PCR ekhwalithi ephezulu ngobukhulu obuhlukene, amandla nezinto zokwakha ukuhlangabezana nezidingo zomcwaningi ngamunye.
Isikhathi sokuthumela: May-17-2023