Ungawakhetha kanjani amapuleti afanele ama-PCR namashubhu wohlelo lwakho lokusebenza?

Ukusabela kwe-Polmerase Chain Reaction (PCR) kuyindlela esetshenziswa kabanzi nge-biology ye-molecular yokukhuliswa kwezingcezwana ze-DNA. I-PCR ifaka izinyathelo eziningana, kufaka phakathi i-denaturation, ukunxusa kanye nokunwetshwa. Impumelelo yale ndlela incike kakhulu kwikhwalithi yamapuleti amapuleti e-PCR namashubhu asetshenzisiwe. Kunezici eziningi okufanele zicatshangelwe lapho ukhetha amapuleti afanele e-PCR namashubhu wohlelo lwakho lokusebenza.Hela izici ezithile okufanele zicatshangelwe:

1. AmandlaAmapuleti we-PCRkanye namashubhu eza ngosayizi kanye namakhono ahlukile. Ukukhethwa kosayizi kanye nomthamo kuncike kakhulu kwinani le-DNA elidinga ukunyuswa ekuphenduleni okukodwa. Isibonelo, uma udinga ukukhulisa inani elincane le-DNA, ungakhetha ishubhu elincane. Uma inani elikhulu le-DNA lidinga ukukhuliswa, ipuleti elinesitha esikhulu lingakhethwa.

2. Amapuleti we-PCR abonakalayo angenziwa ngezinto ezahlukahlukene ezifana ne-polypropylene, i-polycarbonate noma acrylic. I-PolyPropylene iyinto esetshenziswa kakhulu esetshenziswa kakhulu ngenxa yokumelana kwayo kwamakhemikhali nokushisa. Kuyabiza kakhulu uma kuqhathaniswa nezinye izinto. Ama-polycarbonate nama-acrylics abiza kakhulu, kepha abe nokucaca okungcono kakhulu futhi alungele i-PCR yesikhathi sangempela.

I-3. I-TREPAL PROUNTION PCR ifaka imijikelezo eminingi ye-Thermal, edinga ukufudumeza okusheshayo nokupholisa kwengxube yokusabela. Ngakho-ke, amapuleti e-PCR namashubhu kumele abe nokuvuselelwa okuhle okushisayo ukuze aqinisekise ukufudumala okufanayo nokupholisa kwengxube yokusabela. Amapuleti anezindonga ezincanyana nezindawo ezisefulethini zilungele ukukhulisa ukudluliselwa kokushisa.

4. Ukuhambisana namapuleti we-PCR namashubhu kufanele kuhambisane ne-cyper eshisayo oyisebenzisayo. Amapuleti namashubhu kufanele akwazi ukubekezelela amazinga okushisa aphezulu adingekayo ukuze kuthunyelwe kwezingcezu ze-DNA. Hlala uthintana nomkhiqizi we-cyper we-thermal ngamapuleti namashubhu anconyiwe.

I-5. Songa uphawu oluqinile lubalulekile ukuvikela ukungcoliswa kwengxube yokusabela. Amapuleti e-PCR namashubhu angavalelwa kusetshenziswa izindlela ezahlukahlukene ezifana nezimpawu zokushisa, amafilimu anamathela noma amabhodlela. Ukushisa kokushisa kunendlela ephephe kunazo zonke futhi kunikeza isithiyo esiqinile sokulwa nokungcola.

I-6 Ngakho-ke, kufanele babulawe inzalo ngaphambi kokusebenzisa. Kubalulekile ukuthi ukhethe amapuleti namashubhu okulula ukuwela futhi amelana nezindlela zamakhemikhali nezishisayo zokushisa.

Ngamafuphi, ukukhetha ipuleti le-PCR elilungile namashubhu kubalulekile ukuze kukhuliswe ngempumelelo kwe-DNA. Ukukhetha kuncike kakhulu ohlotsheni lwesicelo, inani le-DNA elikhulisiwe, kanye nokuhambisana nabakhi be-Thermal.

I-Suzhou Ace Biomedical Technology Co, Ltd. Inikeza uhla lwamapuleti we-PCR esezingeni eliphakeme namashubhu ngosayizi ahlukahlukene, amakhono nezinto zokwakha ukuze uhlangabezane nezidingo zawo wonke umcwaningi.