Amaphutha ajwayelekile ama-5 ukugwema lapho usebenzisa amathiphu we-pipette kwilebhu
1. Ukukhetha okungalungileIthiphu yePipette
Ukukhetha ithiphu le-pipette elifanele kubalulekile ukunemba nokunemba kokuhlolwa kwakho. Iphutha elilodwa elijwayelekile lisebenzisa uhlobo olungalungile noma usayizi wethiphu ye-pipette. Ithiphu ngayinye yenzelwe izinhlelo ezithile, futhi ukusebenzisa ithiphu engalungile kungaholela emiphumeleni engahambisani futhi kuchithe ama-reagents.
Ukugwema leli phutha, ngaso sonke isikhathi Bheka imihlahlandlela yomenzi noma uthintane nochwepheshe emkhakheni. Cabanga ngezinto ezifana nokuhambisana kwe-PIP nge-pipette, kudingeka ivolumu yesampula, kanye nohlobo lokuhlola olwenzayo. Ngokukhetha ithiphu efanelekayo ye-pipette, ungaqinisekisa ukusebenza kahle kanye nemiphumela ethembekile.
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Ukunamathiselwa okungafanele kwethiphu ye-pipette kungenye iphutha elingahlehlisa ukunemba nokunemba. Uma ithiphu inganamathiselwe ngokuphephile, kungakhulula noma kuvinjelwe ngesikhathi senqubo yePipetting, okuholela ekulahlekelweni kwesampula nokungcola.
Ukuze ugweme lokhu, landela imiyalo yomenzi yokunamathisela ithiphu ye-pipette kahle. Qinisekisa ukuthi ithiphu ifanelana ngokuqinile nangokuphephile ku-pipette nozzle. Ngokwengeziwe, hlola ichopho nganoma yiziphi izimpawu zokugqoka noma ukulimala, bese uyifaka esikhundleni uma kunesidingo. Okunamathiselwe kwethiphu efanelekile kubalulekile emiphumeleni ethembekile nezenzekayo.
3. Ukweqisa noma ukunqamula
I-Pipetting enembile ifaka ukulinganisa ngokucophelela nokudlulisela ivolumu oyifunayo ye-Liquid. Amaphutha amabili ajwayelekile angenzeka phakathi nale nqubo akweqile futhi aqedwe. Ukweqisa kubhekiswa ukweqa ivolumu oyifunayo, ngenkathi ukucindezelwa kusho ukuthi i-Pipetting ngaphansi kwenani elidingekayo.
Womabili lamaphutha angaholela emaphutheni abalulekile emiphumeleni yakho yokuhlola. Ukweqisa kungaholela ekuhlafunweni kwamasampula noma ama-reagents, kanti ukuxhaswa kungaholela ekugxileni okunganele noma ukuhlanganiswa kokuphendula.
Ukugwema ukweqisa noma ukwehlisa amandla, qiniseka ukwenza inqubo efanele yePipetting. Zijwayeze ngokulinganisa kukaPipette kanye nemikhawulo yePipetting. Setha ivolumu ngokufanele, ukuqinisekisa ukuthi i-Pipetting enembile yevolumu oyifunayo. Njalo ulinganise ama-Pipete akho ukuze ulondoloze ukunemba nokunemba.
4. Ukuthinta isitsha sesampula
Ukungcola kuyinkinga enkulu kunoma yisiphi isilungiselelo selebhu. Iphutha elilodwa elijwayelekile ukuthi abacwaningi bakwenza ukuthinta ngengozi isitsha sesampula ngethiphu yePipette. Lokhu kungaletha izinhlayiya zakwamanye amazwe noma izinto zesampula, okuholela emiphumeleni engalungile.
Ukuvimbela leli phutha, ube nengqondo yokunyakaza kwakho futhi ulondoloze isandla esiqinile ngenkathi uPipetting. Gwema ukubeka ingcindezi ngokweqile kwi-pipette noma ukusebenzisa amandla angenasidingo lapho usakaza noma ufisa. Ngokwengeziwe, beka iphuzu eliseduze nendawo ewuketshezi ngaphandle kokuthinta izindonga ezitsheni. Ngokuzijwayeza inqubo enhle yePipetting, unganciphisa ubungozi bokungcoliswa kwesampula.
I-5. Amasu Angalungile E-Dispensing
Iphutha lokugcina lokugwema amasu angalungile asabalalisa. Ukusatshalaliswa okungafanele kungaholela ekusatshalalisweni okungalingani noma okungalingani koketshezi, okuthinta ubuqiniso bemiphumela yakho yokuhlola. Amaphutha ajwayelekile afaka ukusabalalisa okusheshayo noma okungalawulwa, ukudonsa, noma ukushiya amavolumu asele empini.
Ukuqinisekisa ukusabalalisa okunembile futhi okungaguquki, naka ijubane ne-angle ye-pipette ngesikhathi senqubo. Gcina isivinini esilawulwayo nesiqinile, okuvumela uketshezi ukuba lugeleze kahle. Ngemuva kokusabalalisa, linda isikhashana ukuvumela noma yiluphi uketshezi olusele ukuba ushaye ngokuphelele ngaphambi kokususa i-pipette esitsheni.
Ukugwema amaphutha ajwayelekile lapho usebenzisa amathiphu we-Pipette kuLab kubalulekile ukuthola imiphumela ethembekile nezenzekayo. Ngokukhetha ithiphu elifanele le-pipette, likunamathisele kahle, lisebenzisa amasu anembile ama-Pipetting, ukuvimbela ukungcoliswa kwesampula, futhi kuqashe amasu afanelekile okusabalalisa, ungathuthukisa ukunemba kokuhlolwa kwakho.
Isikhathi sePosi: Mar-06-2024