Amaphutha Ama-5 Avamile Okufanele Ugwenywe Lapho Usebenzisa Amathiphu E-Pipette Kulebhu
1. Ukukhetha OkungalungileIthiphu ye-Pipette
Ukukhetha ithiphu elifanele le-pipette kubalulekile ukuze uthole ukunemba nokunemba kokuhlolwa kwakho. Iphutha elilodwa elivamile ukusebenzisa uhlobo olungalungile noma usayizi wethiphu ye-pipette. Ithiphu ngayinye yakhelwe izinhlelo zokusebenza ezithile, futhi ukusebenzisa ithiphu engalungile kungaholela emiphumeleni engahambisani nama-reagents amoshiwe.
Ukuze ugweme leli phutha, hlala ubheka imihlahlandlela yomkhiqizi noma thintana nochwepheshe kulo mkhakha. Cabangela izici ezifana nokuhambisana kwethiphu ne-pipette, umthamo wesampula odingekayo, kanye nohlobo lokuhlolwa okwenzayo. Ngokukhetha ithiphu elifanele le-pipette, ungaqinisekisa ukusebenza kahle kanye nemiphumela ethembekile.
2. Okunamathiselwe Ithiphu Okungafanele
Ukunamathiselwa okungalungile kwephuzu le-pipette kungenye iphutha elingase liphazamise ukunemba nokunemba. Uma ithiphu linganamathiselwe ngokuvikelekile, lingase lixege noma liphume phakathi nenqubo yepayipi, okuholela ekulahlekeni kwesampula kanye nokungcola.
Ukuze ugweme lokhu, landela imiyalelo yomkhiqizi yokunamathisela ithiphu le-pipette ngendlela efanele. Qinisekisa ukuthi ithiphu ilingana ngokuqinile futhi ivikelekile kubhobho ye-pipette. Ukwengeza, hlala uhlole ithiphu ukuze uthole noma yiziphi izimpawu zokuguga noma zokulimala, bese ulishintsha uma kunesidingo. Okunamathiselwe kwethiphu efanele kubalulekile ukuze kube nemiphumela ethembekile futhi ekwazi ukukhiqiza kabusha.
3. Ukubhoboza noma Ukushayelwa Ngaphansi Kombhobho
I-pipetting enembile ihilela ukulinganisa ngokucophelela nokudlulisa umthamo odingekayo woketshezi. Amaphutha amabili ajwayelekile angenzeka phakathi nalolu hlelo wukubhodla amapayipi ngokweqile kanye nokufaka amapayipi angaphansi. I-overpipetting isho ukweqa umthamo ofunekayo, kanti ukuhambisa umhume ongaphansi kusho ukubhobhoza ngaphansi kwenani elidingekayo.
Womabili amaphutha angaholela kumaphutha abalulekile emiphumeleni yakho yokuhlola. Ukukhipha ubhontshisi kungabangela ukuhlanjululwa kwamasampuli noma ama-reagents, kuyilapho ukufakwa ngaphansi kombhobho kungaholela ekugxiliseni okunganele noma izingxube zokusabela.
Ukuze ugweme ukubhoboza amapayipi ngokweqile noma ukufaka amapayipi angaphansi, qiniseka ukuthi usebenzisa indlela efanele yokufaka amapayipi. Zijwayeze ukulinganisa kwe-pipette kanye nemikhawulo yamapayipi. Setha ivolumu ngokufanele, uqinisekise ipayipi elinembile levolumu oyifunayo. Ukulinganisa njalo ama-pipette akho ukuze ugcine ukunemba nokunemba.
4. Ukuthinta Isitsha Sesampula
Ukungcola kuwukukhathazeka okukhulu kunoma iyiphi indawo yaselabhorethri. Iphutha elilodwa elivamile elenziwa abacwaningi ukuthinta ngephutha isitsha sesampula ngethiphu ye-pipette. Lokhu kungethula izinhlayiya zangaphandle noma izinto kusampula, okuholela emiphumeleni engalungile.
Ukuze uvimbele leli phutha, khumbula ukunyakaza kwakho futhi ugcine isandla esiqinile ngenkathi ushaya ipayipi. Gwema ukubeka ingcindezi ngokweqile ku-pipette noma ukusebenzisa amandla angadingekile lapho ukhipha noma ufisa. Ukwengeza, beka ithiphu eduze nendawo ewuketshezi ngaphandle kokuthinta izindonga zesitsha. Ngokuzijwayeza indlela enhle yokufaka amapayipi, unganciphisa ubungozi bokungcola kwesampula.
5. Amasu Okukhipha Okungalungile
Iphutha lokugcina okufanele ligwenywe amasu okukhipha angalungile. Ukukhipha okungalungile kungaholela ekusabalaliseni okuguquguqukayo noma okungalingani koketshezi, okuthinta ukufaneleka kwemiphumela yakho yokuhlola. Amaphutha avamile ahlanganisa ukusabalalisa okusheshayo noma okungalawuleki, ukuconsa, noma ukushiya ngephutha amavolumu asele ethiphu.
Ukuqinisekisa ukusabalalisa okunembile nokuhambisanayo, qaphela isivinini kanye ne-angle ye-pipette ngesikhathi senqubo. Gcina isivinini esilawulwayo nesinyakazayo, uvumele uketshezi ukuthi luhambe kahle. Ngemuva kokukhipha, linda isikhashana esincane ukuze uvumele noma yikuphi uketshezi olusele luphume ngokuphelele ngaphambi kokukhipha i-pipette esitsheni.
ukugwema amaphutha avamile lapho usebenzisa amathiphu e-pipette elebhu kubalulekile ukuze uthole imiphumela ethembekile futhi ekwazi ukukhiqiza kabusha. Ngokukhetha ithiphu le-pipette elilungile, ulinamathisele kahle, usebenzise amasu anembile epayipi, ukuvimbela ukungcoliswa kwesampula, nokusebenzisa amasu okukhipha afanele, ungathuthukisa ukunemba nokunemba kokuhlolwa kwakho.
Isikhathi sokuthumela: Mar-06-2024