I-Pipette sesona sixhobo sixhaphakileyo esisetyenziswa kwiilabhoratri zebhayoloji, zeklinikhi, kunye nohlalutyo apho ulwelo kufuneka lulinganiswe ngokuchanekileyo kwaye ludluliselwe xa kusenziwa udilution, uvavanyo okanye uvavanyo lwegazi. Zifumaneka njenge:
① isitishi esinye okanye izitishi ezininzi
② umthamo osisigxina okanye olungelelanisiweyo
③ ngesandla okanye ngombane
Yintoni i-Single-Channel Pipettes?
I-pipette yesiteshi esisodwa ivumela abasebenzisi ukuba badlulisele i-aliquot enye ngexesha. Ezi zithanda ukusetyenziswa kwiilabhoratri ezinomthamo ophantsi weesampulu, ezinokuthi zisoloko zibandakanyeka kuphando nophuhliso.
Umbhobho wetshaneli enye unentloko enye yokunqwenela okanye ukukhupha amanqanaba achanekileyo olwelo ngokulahlwayo.ingcebiso. Zingasetyenziselwa izicelo ezininzi ngaphakathi kwiilabhoratri ezinomthamo omncinci kuphela. Oku kudla ngokuba ziilabhoratri ezenza uphando olunxulumene nekhemistri yohlalutyo, inkcubeko yeeseli, imfuzo okanye i-immunology.
Yintoni i-Multi-Channel Pipettes?
Iipipette zamatshaneli amaninzi zisebenza ngendlela efanayo neepayipi ezinetshaneli enye, kodwa zisebenzisa ezininziiingcebisoukulinganisa nokukhupha izixa ezilinganayo zolwelo ngaxeshanye. Ukuseta okuqhelekileyo zizi-8 okanye iitshaneli ezili-12 kodwa i-4, 6, 16 kunye ne-48 iiseti zetshaneli zikwakhona. Iinguqulelo ezingama-96 zebhentshiphu yesitishi nazo zinokuthengwa.
Ukusebenzisa i-pipette yamajelo amaninzi, kulula ukugcwalisa ngokukhawuleza i-96-, 384-, okanye i-1,536-kahle.ipleyiti microtiter, enokuthi iqulethe iisampuli zezicelo ezifana ne-DNA amplification, i-ELISA (uvavanyo lokuxilonga), izifundo ze-kinetic kunye nokuhlolwa kwe-molecular.
Ijelo elinye vs. IiPipettes zamatshaneli amaninzi
Ukusebenza kakuhle
I-pipette yetshaneli enye ifanelekile xa usenza umsebenzi wokulinga. Oku kungenxa yokuba ikakhulu kubandakanya ukusebenzisa imibhobho nganye, okanye ungqamaniso olunye ukwenza utofelo-gazi.
Nangona kunjalo, oku ngokukhawuleza kuba sisixhobo esingasebenziyo xa i-output yonyuswa. Xa kukho iisampulu ezininzi / ii-reagents ezigqithiselwayo, okanye iimvavanyo ezinkulu ziyaqhutywa96 iipleyiti zemikrotitre kakuhle, kukho indlela efanelekileyo kakhulu yokudlulisa ulwelo emva koko usebenzisa ipayipi ye-channel enye. Ngokusebenzisa i-pipette yamajelo amaninzi endaweni yoko, inani lamanyathelo epayipi liyancipha kakhulu.
Itheyibhile engezantsi ibonisa inani lamanyathelo okufakela umbhobho ofunekayo kwitshaneli enye, i-8 kunye ne-12 yokucwangcisa ishaneli.
Inani lamanyathelo okufaka imibhobho afunekayo (6 ii-reagents x96 Well Microtitre Plate)
Isitishi esinye iPipette: 576
I-8-Channel Pipette: 72
I-12-Channel Pipette: 48
Umthamo wePipetting
Umehluko omnye obalulekileyo phakathi kwepayipi enye kunye ne-multi-channel yi-volume kwiqula elinokuthi lidluliselwe ngexesha elinye. Nangona kuxhomekeke kumzekelo osetyenziswayo, ngokuqhelekileyo awukwazi ukudlulisa umthamo omkhulu ngentloko nganye kwipipette yamajelo amaninzi.
Umthamo we-pipette ye-channel enye unokudlulisa uluhlu phakathi kwe-0.1ul kunye ne-10,000ul, apho uluhlu lwe-pipette yamajelo amaninzi luphakathi kwe-0.2 kunye ne-1200ul.
Isampulu iyalayisha
Ngokwembali, ii-pipettes ezinamatshaneli amaninzi azizange zilawuleke kwaye kunzima ukuzisebenzisa. Oku kubangele ukulayishwa kwesampulu engahambelaniyo, kunye nobunzima bokulayishaiingcebiso. Kukho iimodeli ezintsha ezikhoyo ngoku, nangona kunjalo, ezisebenziseka ngakumbi kwaye zihamba ngendlela ethile yokulungisa le miba. Kuyafaneleka ukuba uqaphele ukuba nangona ukulayishwa kolwelo kunokungachanekanga ngakumbi ngepayipi ye-channel multi-channel, kunokwenzeka ukuba ichaneke ngakumbi kune-channel enye ngenxa yokungachaneki okwenzekayo kwimpazamo yomsebenzisi ngenxa yokukhathala ( jonga kumhlathi olandelayo).
Ukunciphisa Impazamo Yomntu
Ubunokwenzeka bempazamo yomntu buncitshiswa kakhulu njengoko inani lamanyathelo okubhobhoza liyancipha. Ukwahluka kokudinwa kunye nokukruquka kuyasuswa, okukhokelela kwidatha kunye neziphumo ezithembekileyo kwaye zikwazi ukuphinda zenziwe kwakhona.
Ulungelelwaniso
Ukuqinisekisa ukuchaneka kunye nokuchaneka kwezixhobo zokuphatha ulwelo, ukulinganisa rhoqo kuyadingeka. Umgangatho we-ISO8655 uthi itshaneli ngalinye kufuneka livavanywe kwaye linikwe ingxelo. Okukhona imijelo enayo i-pipette, kokukhona ithatha ixesha elide ukulinganisa okunokutya ixesha.
Ngokutsho kwe-pipettecalibration.net umgangatho we-2.2 wokulinganisa kwi-pipette ye-channel 12 ifuna imijikelo ye-pipetting engama-48 kunye ne-gravimetric weights (i-2 imiqulu x 2 ukuphindaphinda x i-12 channels). Ngokuxhomekeke kwisantya somqhubi, oku kunokuthatha iiyure ezingaphezu kwe-1.5 ngepayipi nganye. Iilebhu zaseUnited Kingdom ezifuna ulungelelwaniso lwe-UKAS kuya kufuneka zenze umlinganiselo opheleleyo wama-360 wegravimetric (imiqulu emi-3 x uphinda-phindo lwe-10 x amajelo ali-12). Ukwenza eli nani lovavanyo ngesandla akunakwenzeka kwaye kunokodlula ugcino lwexesha oluphunyeziweyo ngokusebenzisa i-pipette yamajelo amaninzi kwezinye iilebhu.
Nangona kunjalo, ukoyisa ezi ngxaki iinkonzo zokulinganisa i-pipette ziyafumaneka kwiinkampani ezininzi. Imizekelo yezi yiGilson Labs, iThermoFisher kunye nePipette Lab.
Ukulungisa
Akuyona into abantu abaninzi abacinga ngayo xa kuthengwa i-pipette entsha, kodwa ubuninzi bezinye iipayipi ze-channel ezininzi azikwazi ukulungiswa. Oku kuthetha ukuba itshaneli e-1 yonakele, i-manifold yonke inokufuneka itshintshwe. Nangona kunjalo, abanye abavelisi bathengisa ukutshintshwa kweetshaneli ezizimeleyo, ngoko qiniseka ukuba ukhangele ukulungiswa kunye nomenzi xa uthengela i-pipette yamajelo amaninzi.
Isishwankathelo – Single vs Multi-Channel Pipettes
I-pipette ye-multi-channel sisixhobo esixabisekileyo kwilabhoratri nganye enezinto ezingaphezulu kwe-throughput encinci kakhulu yeesampuli. Phantse kuzo zonke iimeko ubuninzi bomthamo wolwelo olufunekayo kugqithiso lungaphakathi komthamo wento nganyeingcebisokwipipette yamajelo amaninzi, kwaye kukho imiqobo embalwa ehambelana noku. Nakuphi na ukunyuka okuncinci kobunzima ekusebenziseni i-pipette ye-multi-channel igqithiswe kakhulu ngokunciphisa kwenetha kumthwalo wokusebenza, owenziwe ngenani elincitshisiweyo kakhulu lamanyathelo epayipi. Konke oku kuthetha ukuphucula intuthuzelo yomsebenzisi, kunye nokuncipha kwempazamo yomsebenzisi.
Ixesha lokuposa: Dec-16-2022