Kutheni kufuneka izinto ezisetyenziswa kwiLabhoratri ukuze zibe yi-DNase kunye ne-RNase Free?

Kwinkalo yebhayoloji yeemolekyuli, ukuchaneka nokuthembeka kubaluleke kakhulu. Nakuphi na ukungcoliseka kwezinto ezisetyenziswa kwilabhoratri kunokukhokelela kwiziphumo ezigwenxa, ezinokuba neziphumo ezibi kakhulu kuphando lwenzululwazi kunye noxilongo. Omnye umthombo oqhelekileyo wongcoliseko bubukho be-DNase kunye ne-RNase enzymes. Ezi enzymes zithoba i-DNA kunye ne-RNA, ngokulandelanayo, kwaye inokufumaneka kwiimatriki ezahlukeneyo zebhayoloji. Ukunciphisa umngcipheko wosulelo kunye nokuqinisekisa iziphumo ezichanekileyo, izinto ezisetyenziswa kwilabhoratri, njengeiingcebiso zepipette, iipleyiti zequla elinzulu, Iipleyiti zePCR, kunye neetyhubhu, kufuneka i-DNase kunye ne-RNase simahla.

I-DNase kunye ne-RNase enzymes zikho yonke indawo kwaye zifumaneka kwimithombo eyahlukeneyo yebhayoloji, kubandakanywa nomzimba womntu, izityalo, kunye ne-microorganisms. Badlala indima ebalulekileyo kwiinkqubo zeselula ezifana nokuqhekeka kwe-DNA, ukulungiswa kwe-DNA, kunye nokuthotywa kwe-RNA. Nangona kunjalo, ubukho bazo kwindawo yaselabhoratri bunokuba yingozi kwiimvavanyo ezibandakanya uhlalutyo lwe-DNA kunye ne-RNA.

Iingcebiso zePipette zezinye zezona zinto zisetyenziswa kakhulu kwilabhoratri. Zisetyenziselwa ukuphatha ulwelo oluchanekileyo noluchanekileyo, luzenza zibaluleke kakhulu kwizicelo ezahlukeneyo ezinje ngolungiselelo lwesampulu, ulandelelwano lweDNA, kunye nePCR. Ukuba iingcebiso ze-pipette azikho i-DNase kunye ne-RNase ekhululekile, ukungcola kunokwenzeka ngexesha lokubhobhoza, okukhokelela ekuthotyweni kwe-DNA okanye iisampuli ze-RNA. Oku kunokukhokelela kwiziphumo ezingezizo ezingalunganga okanye ezingagqibekanga, ezibeka emngciphekweni ukuthembeka kwalo lonke uvavanyo.

Iipleyiti zequla elinzulu yenye enye ilabhoratri eyimfuneko enokutyiwa, ngakumbi kwizicelo eziphuma phambili. Zisetyenziselwa ukugcinwa kwesampulu, ukuhlanjululwa kwe-serial, kunye nenkcubeko yeeseli. Ukuba ezi cwecwe azikho i-DNase kunye ne-RNase ekhululekile, nayiphi na isampuli ze-DNA okanye i-RNA ezigcinwe kuzo zingangcoliswa, ezikhokelela ekuthotyweni kwe-nucleic acids. Oku kunokuphazamisa ukuchaneka kwezicelo ezisezantsi ezifana ne-PCR, qPCR, okanye ulandelelwano lwesizukulwana esilandelayo.

Ngokufanayo, iipleyiti zePCR kunye neetyhubhu zizinto ezisisiseko kwi-polymerase chain reaction (PCR) izicelo. I-PCR yindlela esetyenziswa kakhulu yokukhulisa ulandelelwano lwe-DNA. Ukuba iiplate ze-PCR kunye neetyhubhu zihlanjululwe nge-DNase okanye i-RNase, inkqubo yokukhulisa inokuthi iphazamiseke, ikhokelela kwiziphumo ezingachanekanga kunye nokutolika kwamanga. I-DNase kunye ne-RNase-free PCR consumables consumables inqanda ukuthotywa kwe-DNA ekujoliswe kuyo okanye i-RNA ngexesha lenkqubo yokukhulisa, iqinisekisa iziphumo ezithembekileyo kunye nokuveliswa kwakhona.

Ukujongana nomba wongcoliseko, izinto ezisetyenziswa kwilabhoratri kufuneka zenziwe ngeenkqubo ezilawulwa kakhulu kunye nezinto eziqinisekisiweyo ukuba ziyi-DNase kunye ne-RNase yasimahla. Iinkampani ezifana neSuzhou Ace Biomedical Technology Co., Ltd., zisebenza ngokukhethekileyo kwimveliso yezinto ezisetyenziswa kwilabhoratri ezihlangabezana nezi mfuno zingqongqo. Njengomvelisi ohamba phambili kwintsimi, i-Suzhou Ace Biomedical Technology Co., Ltd. ibeka phambili umgangatho kunye nokuthembeka.

I-Suzhou Ace Biomedical Technology Co., Ltd. iyayiqonda imeko ebalulekileyo ye-DNase kunye nongcoliseko lwe-RNase kwizinto ezisetyenziswa kwilabhoratri. Iingcebiso zabo ze-pipette, iipleyiti ezinzulu, iipleyiti ze-PCR, kunye neetyhubhu zonke zenziwe zisebenzisa izinto ezikumgangatho ophezulu ezithatha amanyathelo angqongqo okulawula umgangatho wokuqinisekisa ukuba zi-DNase kunye ne-RNase simahla.

Inkampani isebenzisa ubuchule obuphambili bokuvelisa kwaye ithobela imigangatho engqongqo yomgangatho wokuphelisa umngcipheko wosulelo, ngaloo ndlela iqinisekisa iziphumo ezichanekileyo nezithembekileyo kubaphandi kunye noogqirha ngokufanayo. Bayaqonda ukuba nakuphi na ukuthomalalisa kumgangatho wezinto ezisetyenziswa kwilabhoratri kunokuba neziphumo ezide, hayi kuphando kuphela kodwa nakwizicelo zeklinikhi apho uxilongo oluchanekileyo lubalulekile.

Ukuqukumbela, izinto ezisetyenziswa kwilabhoratri ezifana neengcebiso ze-pipette, iipleyiti ezinzulu, iipleyiti zePCR, kunye neetyhubhu kufuneka zibe yi-DNase kunye ne-RNase simahla ukuze kuqinisekiswe ukuchaneka nokuthembeka kovavanyo lwebhayoloji yemolekyuli. Ukungcoliswa kwezi enzyme kunokukhokelela ekuthotyweni kwe-DNA kunye neesampuli ze-RNA, eziphazamisa ukuqinisekiswa kweziphumo ezifunyenweyo. Iinkampani ezifanaI-Suzhou Ace Biomedical Technology Co., Ltd. ukuqonda ukubaluleka kokuveliswa kwezinto ezisetyenziswayo ezihlangabezana nezi mfuno zingqongqo, okwenza ukuba izazinzulu kunye neekliniki ziqhube umsebenzi wazo ngokuzithemba nangokuchaneka.