Kutheni le nto izidlo zalebhu ziyafuneka ukuba zibe yi-dnase kunye ne-rnase yasimahla?

Kwintsimi yebhayoloji yemolekyuli, ukuchaneka nokunyaniseka kubaluleke kakhulu. Nakuphi na ungcoliseko olunemisebenzi lunokukhokelela kwiziphumo eziqinisekisileyo, ezinokuba nemiphumo emibi kuphando lwezenzululwazi kunye noxilongo. Omnye umthombo oqhelekileyo wongcoliseko lubukho be-dnase kunye ne-rnase enzymes. Ezi zinja ze-enzymes zithoba i-DNA kunye ne-RNA, ngokwahlukeneyo, kwaye zinokufumaneka kumatriki eyahlukeneyo. Ukunciphisa umngcipheko wongcoliseko kwaye kuqinisekiswe iziphumo ezichanekileyo, izidlo zelebhu, ezinje ngeIingcebiso zePipette, Iipleyiti ezinzulu, Iipleyiti ze-PCR, kunye neeTubs, kufuneka ibe yi-dnase kunye ne-rnase yasimahla.

I-dnase kunye ne-enzy ye-rozys yi-ibiquitious kwaye inokufumaneka kwimithombo eyahlukeneyo yebhayiloji, kubandakanya umzimba womntu, izityalo, kunye ne-microorganisms. Badlala indima eyimfuneko kwiinkqubo zeselula ezinje ngokuhlanjwa kwe-DNA, ukuLungisa i-DNA, kunye ne-RNA. Nangona kunjalo, ubukho babo kwindawo yelebhu bunokuba yingozi kwiimvavanyo ezibandakanya iDNA kunye nohlalutyo lwe-RNA.

Iingcebiso zepayitha zenye yezona zinto zisetyenziswayo zelebhu eziqhelekileyo. Zisetyenziselwa ukuphatha ngokuchanekileyo nangokufanelekileyo Ukuba iingcebiso zepayitha aziyo-Dnase kunye ne-rnase yasimahla, ungcoliseko lunokwenzeka ngexesha lokumila, kukhokelela ekungcoleni kweDNA okanye iisampulu ze-DNA. Oku kunokubangela iziphumo ezimbi okanye ezingalunganga ezingenamngcipheko, zibeka emngciphekweni ingqibelelo yovavanyo lonke.

Iipleyiti ezinzulu ze-onlate zenye ilabhoratri esebenzayo, ngakumbi kwizicelo ezikhutshelweyo. Zisetyenziselwa ukugcinwa kwesampula, izidlo ze-serial, kunye nenkcubeko yeselfowuni. Ukuba ezi plates azikho dambi kwaye zimahla i-rnase, nayiphi na imizekelo ye-DNA okanye iisampulu ze-RNA ezigcinwe kuzo zinokubangcoliseka, zikhokelela ekungcoleni kwee-acid zenyukliya. Oku kunokuphawula ukuchaneka kwezicelo ezihlathini ezinjenge-PCR, QPCR, okanye ukulandelelana okulandelayo.

Ngokufanayo, iipleyiti ze-PCR kunye neetyhubhu zisisiseko sezinto ezisisiseko kwi-Polymerase Chain Reaction (PCR). I-PCR yindlela esetyenzisiweyo esetyenzisiweyo yokwazisa ukulandelelana kwe-DNA. Ukuba iipleyiti ze-PCR kunye neetyhubhu zingcolisiwe nge-DNAKE okanye i-RNAKIS, inkqubo ye-Amboring inokuthotywa, ikhokelela kwiziphumo ezingachanekanga kunye nokutolikwa kobuxoki. I-DNAIMICH kunye ne-ranase-free i-PRR TOUSTOUS Thintela ukudaniswa kweDNA ekujoliswe kuyo okanye iRNA ngexesha lenkqubo yokuSetyenziswa, ukuqinisekisa iziphumo ezinokuthenjwa kunye nokubuyiswa.

Ukulungisa umba ongcolisayo, izidlo zelebhu kufuneka zenziwe ngeenkqubo ezilawulwa kakhulu kunye nezixhobo eziqinisekisiweyo ukuba zi-DNAME kunye ne-RNAACH. Iinkampani ezinje ngeSuzhou Ace Itekhnoloji CO., Ltd., i-LTD. Njengomvelisi okhokelayo ebaleni, iSuzhou Ace Itekhnoloji ye-CO., LTD. ibeka phambili umgangatho kunye nokuthembeka.

I-Suzhou Ace Itekhnoloji yeBi., Ltd. Iqonda imeko ebaluleke kakhulu ye-Dnase kunye ne-rnase ungcoliseko kwimithwalo yelebhu. Iingcebiso zabo zemibhobho, iipleyiti ezinzulu ezinzulu, iipleyiti ze-PCR, iityhubhu zonke zenziwe zisebenzisa izixhobo ezisemgangathweni ezisemgangathweni ezilawula ukuba ziyi-DNAME kunye neRNAS Sund.

Inkampani isebenzisa ubuchwepheshe bokwenza imveliso kwaye ihambelane nemigangatho emise yomgangatho wokuphelisa umngcipheko wongcoliseko, ngaloo ndlela uqinisekise iziphumo ezichanekileyo nezinokuthenjwa kubaphandi kunye noochwephesha ngokufanayo. Bayakuqonda ukuba nakuphi na ukulalanisa kumgangatho welebhu kungakwazi ukufikelela kude, kungekuphela kuphando kuphela kodwa nakwizicelo zeklinikhi apho isifo esichanekileyo sibalulekile.

Ukuqukumbela, amalabhoratri enokuthathwa njengeengcebiso zepayiphu, iipleyiti ze-PCR, kunye neetyhubhu kufuneka zibe yi-dnase kunye ne-rnase yokuqinisekisa ukuchaneka kunye nokuthembeka kwe-moleculag yovavanyo lwe-moleculager. Ungcoliseko lwezi zinto ze-enzyme zingakhokelela ekungcoleni kweesampulu zeDNA kunye ne-RNNA, ukulalanisa kweziphumo ezifunyenweyo. Iinkampani ezinje ngeI-Suzhou Ace Itekhnoloji yeBhuqu ye-CO., LTD. Ndiyakuqonda ukubaluleka kokwenza izinto ezinokuhlangabezana nezi meko zihlangabezana nezi ngcaciso, zisebenze izazinzulu kunye noogqirha ukuba baqhube umsebenzi wabo ngokuzithemba kunye nokuchaneka.