Sizisebenzisa nini iipleyiti zePCR kwaye sisebenzisa nini iibhubhu zePCR?

Iipleyiti zePCR kunye neeTubhu zePCR: Unokukhetha njani?

I-Suzhou Ace Biomedical Technology Co., Ltd. lishishini elaziwayo elikhethekileyo ekuveliseni izinto ezisetyenziswa kwilabhoratri ezikumgangatho ophezulu. Umnikelo wethu uquka iipleyiti ze-PCR kunye neetyhubhu ezincedisa izazinzulu kwinkalo yebhayoloji yemolekyuli ngophando lwemfuzo kunye novavanyo. Zombini iiplati ze-PCR kunye neetyhubhu zineenzuzo kunye nokungonakali, kwaye ukhetho lwazo zombini luxhomekeke kwiimfuno ezithile zovavanyo.

PCR ipleyiti

Iipleyiti zePCRzi, 96, 384, okanye 1536 iipleyiti kakuhle ezisetyenziselwa nucleic acid amplification, ngokuqhelekileyo yi polymerase chain reaction (PCR). Banomthamo omkhulu, oyimfuneko xa izazinzulu kufuneka zivavanye amakhulu okanye amawaka eisampulu ngaxeshanye. Ifomathi yabo yequla isemgangathweni, nto leyo ekhokelela kukwakheka kwesampulu engaguqukiyo ngaphakathi kwequla ngalinye. Ukuqina kweeplate ze-PCR kuthetha ukuba zingasetyenziswa kwiinkqubo zerobhothi ngaphandle kokuguqulwa.

Ukongeza, iipleyiti ze-PCR zihambelana nezixhobo ezahlukeneyo, kubandakanywa iibhayisikili ze-thermal, abafundi be-fluorescence, kunye ne-PCR sequencers. Kwakhona beza ngemibala eyahlukeneyo, nto leyo enceda abaphandi bagcine umkhondo womsebenzi wabo. Iintlobo ezahlukeneyo zeepleyiti zePCR zisebenzisa izixhobo ezahlukeneyo, kwaye umgangatho weepleyiti nawo awulingani.

ityhubhu yePCR

Iityhubhu ze-PCR ziyi-cylindrical, zifana neetyhubhu ze-eppendorf, kwaye zihlala zinesisombululo se-PCR buffer kunye ne-template DNA. Iityhubhu zovavanyo zihlala zisetyenziswa kwi-PCR kuba zifuna ii-reagents ezimbalwa kuneepleyiti ze-PCR. Oku kubenza ukhetho olulungileyo xa uvavanya iisampuli ezincinci okanye iisayizi ezincinci zeesampulu. Iityhubhu ze-PCR zihlala zihambelana neebhayisikile ze-thermal block block, ezenza zifikeleleke ngakumbi kuneepleyiti.

Iityhubhu ze-PCR zineziphene, ngakumbi xa kuthelekiswa neepleyiti zePCR. Xa kuthelekiswa namacwecwe e-PCR, kulula ukuxuba ngaphandle kokunyuka okungafunekiyo. Ubungakanani babo bukhawulelwe kwimpendulo enye, oku kuthetha ukuba umthamo wesampulu ungaphantsi kweplate yePCR. Ngaphezu koko, azifanelekanga kwiinkqubo zerobhothi, ezinciphisa ukusetyenziswa kwazo kwizicelo eziphezulu.

njani ukukhetha?

Xa ukhetha iipleyiti zePCR kunye neetyhubhu, qwalasela iimfuno ezithile zovavanyo lwakho. Iipleyiti zePCR zilungele uvavanyo lwesampulu ephezulu kunye nomthamo wesampulu ephezulu. Ifomathi esemgangathweni esemgangathweni iqinisekisa iziphumo ezihambelanayo kwipleyiti yonke. Zikwahambelana noluhlu olubanzi lwezixhobo kunye noyilo lwabo oluqinileyo luvumela ukusetyenziswa kunye neenkqubo zerobhothi.

Kwelinye icala, iityhubhu zePCR zikulungele ngakumbi ukuvavanya iisampulu ezincinci okanye ezilinganiselweyo. Zifikeleleka ngakumbi, kwaye ukuhambelana kwazo kunye neebhayisikile zemodyuli ezishushu kuzenza zifikeleleke kubaphandi abaninzi. Zombini iiplate ze-PCR kunye neetyhubhu zineenzuzo kunye nokungonakali, kwaye isigqibo sihla kwiimfuno zovavanyo, uhlahlo lwabiwo-mali, kunye nokulula kumphandi.

ukuququmbela

I-Suzhou Ace Biomedical Technology Co., Ltd ibonelela ngeepleyiti zePCR ezikumgangatho ophezulu kunye neetyhubhu ukuze izazinzulu zizisebenzise kuphando lwazo. Iipleyiti ze-PCR zifanelekile kwizicelo eziphezulu, ngelixa iibhubhu ze-PCR zingcono ukuvavanya iisampuli ezincinci. Ukukhetha phakathi kweepleyiti ze-PCR kunye neetyhubhu kuxhomekeke kwiimfuno ezithile zovavanyo, uhlahlo lwabiwo-mali, kunye nokulula komphandi. Nantoni na isigqibo, iipleyiti ze-PCR kunye neetyhubhu zibonelela ngesisombululo esithembekileyo sovavanyo lwemfuza kunye nophando.