Ukuphendula okuphumelelayo wokukhula, kubalulekile ukuba izinto ezizezinye zokuphendula zikhona kugxininiso oluchanekileyo kwilungiselelo ngalinye. Ukongeza, kubalulekile ukuba kungabikho ungcoliseko.
Ngakumbi xa uninzi lweempendulo kufuneka lusekwe, kusekwe ukuba zilungiselele uMdibaniso obizwa ngokuba ngumxube endaweni yepayitha nganye ngokwahlukeneyo. Imixube eyangqinisisiweyo yangaphambili iyafumaneka kwezorhwebo, apho kuphela izampulu zesampulu. Ngenye indlela, umxube wenkosi unokulungiswa ngokwakho. Kuwo omabini mahluko, umxube usasazwa kwisitya ngasinye se-PCR ngaphandle kwetemplate kwaye isampulu ye-DNA nganye yongezwa ngokwahlukeneyo ekugqibeleni.
Sebenzisa umxube ongumxube uneengenelo ezininzi: Okokuqala, inani lamanyathelo amabini okuhlamba incitshisiwe. Ngale ndlela, zombini umngcipheko weempazamo zabasebenzisi ngexesha lomphunga kwaye umngcipheko wongcoliseko uyancitshiswa kwaye, kunjalo, ixesha lisindisiwe. Kwimigaqo, ukuchaneka kwepayilo kukuphakama, kuba imiqulu emikhulu ikhutshiwe. Oku kulula ukuyiqonda xa ujonge idatha yezobuchwephesha yombhobho: ivolumu encinci, ukonakaliswa kukuphambuka. Isibakala sokuba onke amalungiselelo avela kwinqanawa enye enefuthe elihle kwi-homogeneity (ukuba idityaniswe). Oku kukwaphucula ukuzala kovavanyo.
Xa ulungiselela uMxube wenkosi, ubuncinci i-10% yongezwa ivolumu (umz. Ngale ndlela, (kancinci) Ukungachaneki kwepayilisi, kunye nesiphumo selahleko yesampulu xa isisombululo esinezisombululo esinezicombululo sinokuhlawulwa. Izicoci ziqulathwe kwizisombululo ze-enzyme zizisombululo ezinjengee-Polyproses kunye ne-Master, ibangela ukuba i-foam ye-foam kunye nentsalela kumphezulu wangaphakathiIingcebiso zePipette.
Kuxhomekeka kwisicelo kunye nohlobo lolwelo oluza kusasazwa, indlela echanekileyo yombhobho (1) kufuneka ikhethwe kwaye izixhobo ezifanelekileyo ezikhethiweyo. Kwizisombululo eziqulathe iicoci, inkqubo yokufuduka ngokuthe ngqo okanye ukugcinwa kwe-chie ebizwa ngokuba yi-Pipet Tips ngendlela enye yendlela yokushicilelwa komoya. Isiphumo seI-ACE Pipette Timpoisekwe kwindawo ye-hydrophobic ye-hydrophobic. Utywala obuqulathe izicombululo azishiyi ifilimu yentsalela ngaphakathi nangaphandle, ukuze ukuphulukana nesisombululo kunokuncitshiswa.
Ngaphandle kwento eyenziweyo ngawo onke amacandelo, kubalulekile ukuba akukho lungcoliseko lwamalungiselelo okwenzeka. Akwanelanga ukusebenzisa izinto ezinokucoceka konyulo oluphezulu, kuba inkqubo yokulima kwi-pipette yomoya inokuthi ivelise ii-aerosols ezihlala kumbhobho. I-DNA enokuthi idluliselwe kwi-aerosol inokudluliselwa kwisampulu ukuya kwesinye kwinyathelo elilandelayo le-pipetting kwaye ke likhokelele kungcoliseko. Iinkqubo zokuhambisa ngqo ngqo ezikhankanywe apha ngasentla zinokunciphisa kwakhona lo mngcipheko. I-pipettes ye-cudional ye-hushiion ivakala ukuba isebenzise iingcebiso zokuhluza ukukhusela i-pipette cone ngokugcina i-spashes, i-aerosols, kunye nee-biomolecules.
IXESHA LOKUQALA: I-DEC-06-2022