Yintoni i-vitro yokugcina isifo?

Kwi-vitro diacnostics ibhekisa kwinkqubo yokuchonga isifo okanye imeko ngokuqhelisela iisampulu zebhayoloji ngaphandle komzimba. Le nkqubo ixhomekeke kakhulu ngeendlela ezahlukeneyo zemolekyuli, kubandakanya i-PCR kunye nokukhutshwa kwe-nucleic. Ukongeza, ukuphathwa kwe-fluid yinto ebalulekileyo ye-vitro diacnostics.

I-PCR okanye i-polymerase chain impendulo isetyenziselwa ukuphakamisa iziqwenga ezithile zeDNA. Ngokusebenzisa i-prims ezithile, i-PCR ivumela ukwahlula-hlulwa okukhethiweyo kulandelelwano lwe-DNA, olunokuhlaziywa ngemiqondiso yesifo okanye usulelo. I-PCR isetyenziselwa ukufumana intsholongwane, i-bacterial, i-fungal kunye nosulelo lweparasitic, kunye nezifo zemfuza kunye nomhlaza.

Ukukhutshwa kwe-nucleic straction yindlela esetyenziselwa ukwahlula kwaye icoce iDNA okanye i-RNA kwiisampulu zebhayoloji. I-acid ekhutshiweyo i-nucleic ifumaneka ngolungelelwano olungelulo, kubandakanya i-PCR. Ukukhutshwa kwe-nucleic ibalulekile kucwangciso oluchanekileyo kunye nokucwangciswa konyango kwizifo ezahlukeneyo kunye neemeko.

Ukuphathwa kwamanzi yinkqubo ebandakanya ukudluliselwa ngokuchanekileyo, ukusasazwa nokudityaniswa kwenani le-lwimi kwindawo yelebhu. Iinkqubo zokuphatha okuzenzekelayo ze-autoted i-autoted i-autoted i-autoted i-autoted i-autoted i-omet ye-autoted i-omet ye-autoted i-omet ye-autoted i-owele

Kwi-vitro disnostics ixhomekeke kakhulu kwezi ndlela zebhayoloji zemolekyuli kuba zivumela ukufunyanwa kunye nohlalutyo lwezifo zemfuza kunye nemolekyuli. Umzekelo, i-PCR isetyenziselwa ukukhupha ukulandelelana okukhethekileyo kwe-gene ehambelana nomhlaza webele, ngelixa ukuthwala i-nucleic straction kungasetyenziselwa ukwahlula i-DNA ye-tumoor esuka kwiisampulu zegazi.

Ukongeza kwezi ndlela, ezinye iindlela ezahlukeneyo kunye nezixhobo zisetyenziswa kwi-vitro yocingela. Umzekelo, izixhobo ze-microfluidic ziya zisetyenziswa ngokukuko kwiindlela eziphezulu kunye nezicelo zononophelo. Ezi zixhobo zenzelwe ukuba ziphathe kakuhle kwaye zixhase ubungakanani obuncinci bezitywala, zibenzela ukuba zilungele i-PCR kunye nezinye iinkqubo zemolekyuli zemolekyuli.

Ngokufanayo, ukulandelelana kwetekhnoloji elandelayo (i-NG) idlala indima ebaluleke kakhulu kwi-VITRO. Ii-NGO zenza ukuba ukulandelelana kwezigidi zeziqwengana zeDNA, ukuvumela ukufunyanwa ngokukhawuleza nokuchanekileyo kokuguqulwa kohlobo lwendalo. I-NGS inamandla okuguqula isifo kunye nokunyanga izifo zemfuza kunye nomhlaza.

Kwisishwankathelo, kwi-vitro diacnostics yinxalenye ebalulekileyo yonyango lwanamhlanje kwaye ixhomekeke kakhulu kwiindlela ze-molecular ezifana ne-PCR, i-sccleic yokukhupha, kunye nokuphatha amanzi. Ezi teknoloji, kunye neetekhnoloji ezinje ngezixhobo ze-microfluidic kunye nee-NG, zitshintsha indlela esichongiweyo kunye nokunyanga izifo. Njengoko itekhnoloji iyaqhubeka ukuqhubela phambili, kwi-vitro diacnostics kunokwenzeka ukuba ibe yeyona ichanekile kwaye isebenza kakuhle, ekugqibeleni iphumelele iziphumo kunye nomgangatho wobomi.

At Suzhou ace biomedical,Sinikezelwe kukubonelela ngelona shishini liphezulu lelebhu kuzo zonke iimfuno zakho zesayensi. Uluhlu lwethu lweengcebiso zepatte, iipleyiti ze-PCR, i-PUBR Tubs, kunye nefilimu zenziwe ngendlela efanelekileyo kwaye yenzelwe ukuqinisekisa ukuchaneka kunye nokuchaneka kuzo zonke iimvavanyo zakho. Iingcebiso zethu zepayiphu zihambelana nazo zonke iimpawu ezinkulu zemibhobho kwaye zize kwisayizi eyahlukeneyo ukulungiselela iimfuno zakho ezithile. Iipleyiti zethu ze-PCR kunye neetyhubhu zenziwa kwizinto eziphezulu eziphezulu kwaye zenzelwe ukumelana nemijikelezo emininzi ye-thermal ngelixa igcina ingqibelelo yesampulu. Ifilimu yethu yokutywina inika itywina eliqinileyo ukuthintela ukuphuma kunye nokungcoliseka kwezinto ngaphandle. Siyakuqonda ukubaluleka kweLebhu ethembekileyo nefanelekileyo, yiyo ukuba kutheni sizabalazela ukukunika ezona mveliso kunye neenkonzo ezilungileyo zinokwenzeka. Iqela lethu leengcali lihlala likhona ukukunceda kuyo nayiphi na imibuzo okanye iinkxalabo onokuba nazo.