yintoni ukuxilongwa kwe-in vitro?

I-in vitro diagnostics ibhekisa kwinkqubo yokufumanisa isifo okanye imeko ngokuhlela iisampulu zebhayoloji ezivela ngaphandle komzimba. Le nkqubo ixhomekeke kakhulu kwiindlela ezahlukeneyo zebhayoloji yemolekyuli, kubandakanya iPCR kunye ne-nucleic acid extraction. Ukongeza, ukuphathwa kwamanzi yinxalenye ebalulekileyo yokuxilongwa kwe-in vitro.

I-PCR okanye i-polymerase chain reaction bubuchule obusetyenziselwa ukukhulisa amaqhekeza athile e-DNA. Ngokusebenzisa i-primers ethile, i-PCR ivumela ukukhulisa okukhethiweyo kokulandelelana kwe-DNA, enokuthi emva koko ihlalutye iimpawu zesifo okanye ukusuleleka. I-PCR isetyenziswa ngokuqhelekileyo ukufumanisa izifo zentsholongwane, iibhaktheriya, i-fungal kunye ne-parasitic, kunye nezifo zofuzo kunye nomhlaza.

I-Nucleic acid extraction bubuchule obusetyenziselwa ukwahlula kunye nokucoca i-DNA okanye i-RNA kwiisampuli zebhayoloji. I-nucleic acids ekhutshiweyo emva koko iyafumaneka ukuhlalutya ngakumbi, kubandakanywa ne-PCR. I-Nucleic acid extraction iyimfuneko yokuxilongwa ngokuchanekileyo kunye nokucwangciswa kwonyango kwizifo ezahlukeneyo kunye neemeko.

Ukuphathwa kolwelo yinkqubo ebandakanya ukhutshelo oluchanekileyo, ukukhupha kunye nokuxuba imiyinge emincinci yolwelo kwindawo yaselabhoratri. Iinkqubo zokuphatha ulwelo oluzenzekelayo ziye zanda kakhulu kwiminyaka yamva nje njengoko zivumela ukuhamba okuphezulu kunye nokuchaneka okukhulu kwiimvavanyo ezifana ne-PCR kunye ne-nucleic acid extraction.

Uxilongo lwe-in vitro luxhomekeke kakhulu kobu buchule bebhayoloji yemolekyuli kuba ivumela ukuchongwa kunye nohlalutyo lweempawu ezinxulumene nesifo sofuzo kunye nemolekyuli. Ngokomzekelo, i-PCR ingasetyenziselwa ukukhulisa ulandelelwano oluthile lwemfuza oluhambelana nomhlaza webele, ngelixa i-nucleic acid extraction ingasetyenziselwa ukwahlula i-DNA ephuma kwi-tumor kwiisampuli zegazi.

Ukongeza kobu buchule, ezinye iindlela ezahlukeneyo kunye nezixhobo zisetyenziswa kwi-in vitro diagnostics. Ngokomzekelo, izixhobo ze-microfluidic zisetyenziswa kakhulu kwi-high-throughput kunye ne-point-of-care-care applications. Ezi zixhobo ziyilelwe ukuphatha ngokuchanekileyo kunye nokusebenzisa izixa ezincinci zolwelo, zizenze zilungele i-PCR kunye nezinye iinkqubo zebhayoloji yemolekyuli.

Ngokunjalo, itekhnoloji yesizukulwana esilandelayo sokulandelelana (NGS) idlala indima ebaluleke kakhulu kuxilongo lwe-vitro. I-NGS yenza ulandelelwano olunxuseneyo lwezigidi zamaqhekeza e-DNA, ivumela ukufunyaniswa okukhawulezileyo nokuchanekileyo koguquko lwemfuzo olunxulumene nesifo. I-NGS inamandla okuguqula ukuxilongwa kunye nonyango lwezifo zemfuzo kunye nomhlaza.

Isishwankathelo, ukuxilongwa kwe-in vitro yinxalenye ebalulekileyo yamayeza anamhlanje kwaye kuxhomekeke kakhulu kubuchule bebhayoloji yemolekyuli njengePCR, i-nucleic acid extraction, kunye nokuphathwa kolwelo. Ezi teknoloji, kunye nobuchwepheshe obufana nezixhobo ezincinci kunye ne-NGS, ziyayitshintsha indlela yokuxilonga kunye nokunyanga izifo. Njengoko itekhnoloji iqhubeka nokuhambela phambili, ukuxilongwa kwe-vitro kunokwenzeka ukuba kuchaneke ngakumbi kwaye kusebenze, ekugqibeleni kuphuculwe iziphumo zesigulana kunye nomgangatho wobomi.

At Suzhou Ace Biomedical,sizinikele ekukuboneleleni ngezona zinto zisemgangathweni ophezulu welebhu kuzo zonke iimfuno zakho zenzululwazi. Uluhlu lwethu lweengcebiso ze-pipette, iipleyiti ze-PCR, iityhubhu ze-PCR, kunye nefilimu yokutywina ziyilwe ngokucokisekileyo kwaye zenzelwe ukuqinisekisa ukuchaneka kunye nokuchaneka kuzo zonke iimvavanyo zakho. Iingcebiso zethu ze-pipette zihambelana nazo zonke iimpawu ezinkulu ze-pipettes kwaye ziza ngobukhulu obuhlukeneyo ukuze zilungele iimfuno zakho ezithile. Iipleyiti zethu zePCR kunye neetyhubhu zenziwe ngezinto ezikumgangatho ophezulu kwaye ziyilelwe ukuba zimelane nemijikelo emininzi ye-thermal ngelixa igcina ingqibelelo yesampulu. Ifilimu yethu yokutywina ibonelela ngesitywina esiqinileyo ukuthintela ukuvela komphunga kunye nokungcoliswa kwezinto zangaphandle. Siyakuqonda ukubaluleka konikezelo lwaselebhu oluthembekileyo nolusebenzayo, yiyo loo nto sizama ukukunika ezona mveliso kunye neenkonzo ezingcono kangangoko sinakho. Iqela lethu leengcaphephe lihlala likhona ukukunceda ngayo nayiphi na imibuzo okanye izinto ezikuxhalabisayo onokuba nazo.