Yintoni ipleyiti yePCR?

I-PCR plate luhlobo lwe-primer, i-dNTP, i-Taq DNA polymerase, i-Mg, i-template nucleic acid, i-buffer kunye nabanye abathwali ababandakanyekayo kwi-amplification reaction kwi-Polymerase Chain Reaction (PCR).

1. Ukusetyenziswa kwe-PCR plate

Isetyenziswa ngokubanzi kwiinkalo zemfuza, i-biochemistry, i-immunity, iyeza, njl., kungekhona nje kuphando olusisiseko olufana ne-gene isolation, i-cloning kunye ne-nucleic acid uhlalutyo lokulandelelana, kodwa kunye nokuxilongwa kwezifo okanye nayiphi na indawo apho kukho i-DNA. kunye ne-RNA. Yinto yexesha elinye esetyenziswa kwilabhoratri. Imveliso.

2.96 Kulungile PCRIzinto zepleyiti

Izinto zayo ziyi-polypropylene (PP) kule mihla, ukwenzela ukuba ikwazi ukulungelelanisa ngokuphindaphindiweyo kwimimiselo yokushisa ephezulu kunye nephantsi kwindlela yokusabela kwe-PCR, kwaye inokufikelela kwiqondo lokushisa eliphezulu kunye noxinzelelo oluphezulu. Ukuze ufezekise umsebenzi ophezulu ngokubambisana nompu womqolo, umatshini we-PCR, njl., iipleyiti ze-PCR ezingama-96 okanye ezingama-384 zisetyenziswa ngokuqhelekileyo. Ubume bepleyiti buhambelana nomgangatho wamazwe ngamazwe we-SBS, kwaye ukuze ulungelelanise noomatshini be-PCR abavelisi abahlukeneyo, inokwahlulwa ibe ziindlela ezine zokuyila: akukho siketi, isiqingatha sesiketi, isiketi esiphakamileyo kunye nesiketi esigcweleyo ngokwendlela yoyilo lwesiketi.

3. Umbala oyintloko we-PCR plate

Eziqhelekileyo ziyabonakala kwaye zimhlophe, phakathi kwazo iipleyiti zePCR ezimhlophe zifaneleke ngakumbi kwi-PCR yexesha lokwenene le-fluorescent quantitative.