Zeziphi izilumkiso ekulinganiseni i-pipette kunye ne-burette?
Umlinganiselo ochanekileyo wolwelo uyimfuneko kwimifuniselo yelabhoratri eyimpumelelo, ngakumbi kwimimandla efana nayouphando lwebhayoloji, ikhemistri, kwayeamayeza. Ulungelelwaniso lwezixhobo ezifanaiipipetteskwayeii-burettekubalulekile ekuqinisekiseni ukuchaneka komsebenzi wakho. Nokuba wenza ii-titrations, udlulisa ulwelo, okanye wenza uhlalutyo lweekhemikhali, ukulandela izilumkiso ezifanelekileyo ngexesha lenkqubo yokulinganisa kuqinisekisa iziphumo ezingaguqukiyo, ezithembekileyo.
At Ace Biomedical, siyakuqonda ukubaluleka kokusebenzisa izixhobo ezikumgangatho ophezulu kwimilinganiselo echanekileyo. Kweli nqaku, siya kukukhokela kwezona ndlela zilungileyo kunye nezilumkiso eziphambili zokulinganisa ii-pipettes kunye nee-burettes, ukuqinisekisa ukuchaneka okuphezulu kunye nokusebenza.
Ukuphonononga wethuiingcebiso zepipettekunye nezinye izixhobo ezichanekileyo, tyelela yethuiphepha lemvelisookanye ufunde ngakumbi malunga wethuiinkonzo.
Yintoni iCalibration kwaye kutheni ibalulekile?
Ukulinganisa kubhekiselele kwinkqubo yokuqinisekisa ukuchaneka kwezixhobo zebhubhoratri ngokuthelekisa imilinganiselo yazo kunye nemigangatho eyaziwayo. Kwii-pipettes kunye ne-burettes, oku kuthetha ukuqinisekisa ukuba umthamo abawulinganiselayo okanye abawuhambisayo uhambelana nemali ejoliswe kuyo ngokusondeleyo. Ngaphandle kokulungelelaniswa okufanelekileyo, imilinganiselo engachanekanga inokukhokelela kwiziphumo zovavanyo eziphosakeleyo, yiyo loo nto ukulinganisa rhoqo kubalulekile.
Imiqathango yokuLungelelanisa iPipette
I-pipette sisixhobo esichanekileyo esisetyenziselwa ukudlulisa umthamo othile wolwelo. Ukuqinisekisa ukuchaneka kwayo, landela la manyathelo okhuseleko abalulekileyo ngexesha lokulinganisa:
1. Qinisekisa ukuba iPipette icocekile
Ucoceko lubalulekile xa ulinganisa ipayipi. Nayiphi na intsalela okanye ukungcola okushiywe ngaphakathi kwepipette ukusuka ekusebenziseni kwangaphambili kunokutshintsha umlinganiselo. Coca i-pipette yakho ngokucokisekileyo kunye ne-arhente yokucoca efanelekileyo kwaye uyihlambulule ngayomamanzi amisiweukuqinisekisa ukuba akukho zikhemikhali ezihlala zihleli.
2. Cinga ngeeMpembelelo zoBubushushu kuMqulu
Ubushushu buchaphazela kakhulu umthamo wolwelo. Ukulinganisa kufuneka kuqhutywe kwiqondo lokushisa elifanayo apho i-pipette iya kusetyenziswa khona. Uninzi lweepipettes zilinganiswe kwiqondo lokushisa eliqhelekileyo20°C ukuya kuma-25°C. Ukuba iqondo lokushisa lolwelo liyahluka kolu luhlu, kunokuchaphazela umthamo okhutshwayo. Qinisekisa ukuba zombini i-pipette kunye ne-liquid ikwiqondo lokushisa elihambelanayo ukuze ugweme ukungafani.
3. Susa amaqamza oMoya
Amaqamza omoya ngaphakathi kwipipette anokubangela iimpazamo ezinkulu zokulinganisa. Ngaphambi kokulinganisa, qiniseka ukuba akukho amaqamza omoya kumbhobho wepipette okanye ithiphu. Thepha i-pipette ngobumnene okanye uyifake ukuze ususe nawuphi na umoya obanjwe. Oku kuya kuqinisekisa ukuba i-pipette ikhupha umthamo ochanekileyo wolwelo.
4. Sebenzisa ubuChwephesha bokuPhatha ngokufanelekileyo
Indlela yokusingatha i-pipette ngexesha lokulinganisa inokuchaphazela ngokuthe ngqo ukuchaneka komlinganiselo. Hlala ubambe i-pipette ngokuthe nkqo ukuqinisekisa ukuhamba kolwelo okungaguqukiyo. Ukuthobisa i-pipette kunokukhokelela kwiimpazamo kwivolumu, ngoko ke kubalulekile ukuyiphatha ngokwemiyalelo yomenzi.
5. Khangela umonakalo obonakalayo
Ngaphambi kokulinganisa, khangela i-pipette umonakalo obonakalayo, njengokuqhekeka okanye ukuvuza. Nawuphi na umonakalo ungakhokelela kwimilinganiselo engachanekanga kwaye kufuneka ilungiswe ngokukhawuleza. I-pipette eyonakalisiweyo ayifanelekanga ukulinganisa ngokuchanekileyo, ngoko qinisekisa ukuba ikwimeko efanelekileyo ngaphambi kokusetyenziswa.
6. Sebenzisa ii-Calibration Liquids ezaziwayo
Ukulinganisa i-pipette, sebenzisa i-liquid enomthamo owaziwayo, njengayemamanzi amisiwe. Ukulinganisa ulwelo olukhutshwa yipipette kwaye uthelekise nexabiso elilindelekileyo. Ukuba kukho ukungafani, lungisa i-pipette ukuze ufanise umthamo ochanekileyo. Iitshekhi zokulinganisa rhoqo ziya kunceda ukugcina ukuchaneka kwexesha.
7. Gcina iPipette ngokuchanekileyo
Ukugcinwa okufanelekileyo kubalulekile ukugcina ukulinganisa kwepipette yakho. Xa ingasetyenziswa, gcina i-pipette kwindawo ekhuselekileyo, eyomileyo, kude neekhemikhali ezinzima kunye nomonakalo womzimba. Ukusebenzisa ityala elikhuselayo okanye umnikazi uqinisekisa ukuba i-pipette ihlala kwindawo efanelekileyo yokusetyenziswa kwixesha elizayo.
Izilumkiso zokulinganisa i-burette
I-burette iqhele ukusetyenziselwa ukukhupha umthamo ochanekileyo wolwelo ngexesha le-titrations okanye eminye imifuniselo. Ukulungelelaniswa okufanelekileyo kwe-burette kufuna ingqalelo ngenyameko kwiinkcukacha. Ngezantsi kukho izilumkiso eziphambili ekufuneka uzilandele xa ulinganisa i-burette:
1. Coca i-burette ngokucokisekileyo
Kanye njenge-pipette, i-burette kufuneka icocwe ngaphambi kokulinganisa. Nayiphi na intsalela evela kwiimvavanyo zangaphambili inokuphazamisa umlinganiselo. Coca i-burette ngokucokisekileyomamanzi amisiwekwaye uyihlambulule kaninzi ukuze ususe nakuphi na ungcoliseko.
2. Jonga amaqamza oMoya
Amaqamza omoya ngaphakathi kwe-burette okanye umbhobho angakhokelela kwiimpazamo ezibalulekileyo zokulinganisa. Ngaphambi kokulinganisa, qiniseka ukuba akukho amaqamza omoya. Gcwalisa i-burette ngamanzi, kwaye uvumele nawuphi na umoya ovalelweyo ukuba ubaleke ngokuvula i-stopcock, emva koko ukhuphe ulwelo ukucima amaqamza.
3. Zero the Burette
Ukunciphisa i-burette linyathelo elibalulekileyo lokulinganisa. Xa i-burette igcwele, qiniseka ukuba indawo yokuqala ibekwe kuyoInqaku elinguziro. Nakuphi na ukuphambuka kwinqanaba le-zero kunokubangela ukungachaneki kumlinganiselo womthamo ngexesha lokusetyenziswa. Ngalo lonke ixesha jonga i-burette iku-zero phambi kokuba uqale naluphi na uvavanyo okanye inkqubo yokulinganisa.
4. Sebenzisa ii-Calibration Liquids ezaziwayo
Njengakwi-pipettes, ukulinganisa i-burette usebenzisa imigangatho eyaziwayo yokuchaneka.Mamanzi amisiwelulwelo olufanelekileyo ukulungiselela le njongo kuba lunoxinaniso olwaziwayo kwaye kulula ukululinganisa. Emva kokuzalisa i-burette, uchithe i-liquid kwi-cylinder ephumelele kwaye uqhathanise umthamo kwixabiso elilindelekileyo. Lungisa ulungelelwaniso lwe-burette ukuba kukho ukungangqinelani kufunyenwe.
5. Hlola iStopcock
I-stopcock ilawula ukuhamba kolwelo olusuka kwi-burette. Qinisekisa ukuba isebenza ngokufanelekileyo kwaye ayinakuvuza. I-stopcock engasebenzi kakuhle inokubangela ukuhamba okungalinganiyo, okukhokelela kufundo olungachanekanga. Shintsha okanye ulungise istopcock ukuba kukho imfuneko ukuqinisekisa ukusebenza kakuhle.
6. Beka i-Burette ngokuthe nkqo
Ukufezekisa imilinganiselo echanekileyo, qiniseka ukuba i-burette ibekwe ngokuthe nkqo ngexesha lokulinganisa. Ukutyekisa i-burette kunokubangela ukuba ulwelo luhambe ngokungalinganiyo, okukhokelela kwiimpazamo. Sebenzisa i-burette stand ukuze ugcine i-burette ngokukhuselekileyo kwaye ugcine ukulungelelaniswa kwayo ngokuthe nkqo ngexesha lokulinganisa.
7. Funda iMeniscus ngokuchanekileyo
Xa ufunda inqanaba le-liquid kwi-burette, qinisekisa ukuba ukhonakwinqanaba lamehlokunye ne-meniscus. I-meniscus yindawo egobileyo yolwelo, kwaye kuninzi lwamanzi njengamanzi, ijika liya kuba lihle. Funda indawo ephantsi ye-meniscus ukuqinisekisa ukufundwa kwevolumu ngokuchanekileyo.
Ukulinganisa rhoqo kwee-pipettes kunye nee-burettes kubalulekile ekufezekiseni imilinganiselo echanekileyo, echanekileyo kwiimvavanyo zebhubhoratri. Ngokulandela la manyathelo okhuseleko angentla, uyaqinisekisa ukuba izixhobo zakho zibonelela ngedatha ethembekileyo ngexesha ngalinye. Nokuba usebenza ngaphakathiuphando lwebhayoloji, uhlalutyo lwekhemikhali, okanyeuvavanyo lwamayeza, imilinganiselo yolwelo echanekileyo ibalulekile kwimpumelelo yovavanyo lwakho.
At Ace Biomedical, siyakuqonda ukubaluleka kwezixhobo zelabhoratri ezikumgangatho ophezulu. Yethu iingcebiso zepipette kunye nezinye iimveliso ziyilelwe ukuhlangabezana nemigangatho ephezulu yokuchaneka, ukuqinisekisa ukuba imifuniselo yakho inika iziphumo ezingcono. Ngolwazi oluthe vetshe, ndwendwela wethuiphepha lasekhaya, okanye ukuba ufuna uncedo, zive ukhululekileQhagamshelana nathi.
Ixesha lokuposa: Dec-27-2024