Izinto ezibalulekileyo kufuneka ziqwalaselwe xa uvala inzalaIingcebiso zePipette? Makhe sijonge kunye.
1. Nciphisa incam ngephephandaba
Yifake kwibhokisi yencam yokuvala ubushushu obumanzi, i-121 degrees, i-1bar yoxinzelelo lwe-atmospheric, imizuzu engama-20; ukuze ugweme ingxaki yomphunga wamanzi, unokusonga ibhokisi yencam ngephephandaba, okanye uyibeke kwi-incubator emva kokuvala inzala ukuze yome.
2. Xa i-autoclaving, i-tip box ifanele isongelwe kwiphephandaba ukwenzela ukuvala inzala
Iingubo zephephandaba zinokufunxa amanzi kwaye zinokuphepha amanzi amaninzi, eyona nto ibalulekileyo kukuthintela ukungcoliseka kwakhona.
3. Imiba efuna ingqalelo kwi-sterilization yeengcebiso ze-pipette ngexesha lokukhutshwa kwe-RNA
Sebenzisa iityhubhu zeEP eziqhelekileyo kunye neengcebiso zepipette. Ngaphambi kokuba i-autoclaving, zicwilise emanzini e-DEPC ubusuku bonke ukuze ususe i-RNase. Emva kokususa i-DEPC ngosuku olulandelayo, zibeke kwibhokisi yencam ye-pipette yokuvala ubushushu obumanzi. I-121 degrees, imizuzu eyi-15-20. Ukuze uthintele iingxaki zomphunga wamanzi, amaphephandaba asenokugqunywa kwibhokisi yencam, okanye abekwe kwi-incubator ukuze ome emva kokuvalwa inzala. Kungcono ukucoca inzala ngokuthe ngqo phambi kokutsalwa, kwaye ungasebenzisi iingcebiso ze-pipette ezinde ukukhupha i-RNA.
Izinto eziluncedo ngobushushu obuphezulu bokuvala inzala:
Ukungena kobushushu obunamandla ngomphunga; ubuchule obuphezulu bokuvala inzala; ixesha elifutshane lokuvala inzala; akukho kungcoliseka kweekhemikhali okanye emzimbeni ngexesha lenkqubo yokuvala inzala; iiparitha ezimbalwa zokulawula izixhobo zokuvala inzala kunye nokusebenza okuzinzile; ukuvala umphunga kusetyenziselwa ukonga amanzi kunye namandla. Ukusebenza okuphezulu kwe-thermal.
Iingcebiso ze-pipette ze-Yongyue zenziwe nge-polypropylene (PP) yebakala lonyango, edibana ne-USP VI ibakala, inokumelana nekhemikhali ebalaseleyo, kwaye inokuvalwa inzala kwiqondo lobushushu obuphezulu obuyi-121 kunye noxinzelelo oluphezulu (unyango lwe-electron beam sterilization).
1. Nciphisa incam ngephephandaba
Yifake kwibhokisi yencam yokuvala ubushushu obumanzi, i-121 degrees, i-1bar yoxinzelelo lwe-atmospheric, imizuzu engama-20; ukuze ugweme ingxaki yomphunga wamanzi, unokusonga ibhokisi yencam ngephephandaba, okanye uyibeke kwi-incubator emva kokuvala inzala ukuze yome.
2. Xa i-autoclaving, i-tip box ifanele isongelwe kwiphephandaba ukwenzela ukuvala inzala
Iingubo zephephandaba zinokufunxa amanzi kwaye zinokuphepha amanzi amaninzi, eyona nto ibalulekileyo kukuthintela ukungcoliseka kwakhona.
3. Imiba efuna ingqalelo kwi-sterilization yeengcebiso ze-pipette ngexesha lokukhutshwa kwe-RNA
Sebenzisa iityhubhu zeEP eziqhelekileyo kunye neengcebiso zepipette. Ngaphambi kokuba i-autoclaving, zicwilise emanzini e-DEPC ubusuku bonke ukuze ususe i-RNase. Emva kokususa i-DEPC ngosuku olulandelayo, zibeke kwibhokisi yencam ye-pipette yokuvala ubushushu obumanzi. I-121 degrees, imizuzu eyi-15-20. Ukuze uthintele iingxaki zomphunga wamanzi, amaphephandaba asenokugqunywa kwibhokisi yencam, okanye abekwe kwi-incubator ukuze ome emva kokuvalwa inzala. Kungcono ukucoca inzala ngokuthe ngqo phambi kokutsalwa, kwaye ungasebenzisi iingcebiso ze-pipette ezinde ukukhupha i-RNA.
Izinto eziluncedo ngobushushu obuphezulu bokuvala inzala:
Ukungena kobushushu obunamandla ngomphunga; ubuchule obuphezulu bokuvala inzala; ixesha elifutshane lokuvala inzala; akukho kungcoliseka kweekhemikhali okanye emzimbeni ngexesha lenkqubo yokuvala inzala; iiparitha ezimbalwa zokulawula izixhobo zokuvala inzala kunye nokusebenza okuzinzile; ukuvala umphunga kusetyenziselwa ukonga amanzi kunye namandla. Ukusebenza okuphezulu kwe-thermal.
Iingcebiso ze-pipette ze-Yongyue zenziwe nge-polypropylene (PP) yebakala lonyango, edibana ne-USP VI ibakala, inokumelana nekhemikhali ebalaseleyo, kwaye inokuvalwa inzala kwiqondo lobushushu obuphezulu obuyi-121 kunye noxinzelelo oluphezulu (unyango lwe-electron beam sterilization).
Ixesha lokuposa: Nov-02-2021