Ngophando lwemfuza kunye nonyango, i-Polymerase Chain Rein (PCR) yindlela eqhelekileyo esetyenzisiweyo yokwazisa iisampulu zeDNA zovavanyo ezahlukeneyo. Le nkqubo ixhomekeke kakhulu kwizicelo ze-PCR ezinokufumana uvavanyo oluphumelelayo. Kweli nqaku, sixubusha ngezinto ezingabalulekanga ukuze kuvavanywe ngokugqibeleleyo kwePRCR:
Iplate ye-PCR:
Iipleyiti ze-PCR zenye yezona zinto zininzi zibaluleke kakhulu kulo naluphi na uvavanyo lwe-PCR. Ziyilelwe ukuba zenze iqondo lokushisa ngokukhawuleza kwaye linikezele ngokutsha lwamaSum Iipleyiti ziyafumaneka kwiintlobo ngeentlobo zobulungisa ezibandakanya i-96-kakuhle, i-384-kunye ne-1536 - kakuhle.
Iipleyiti ze-PCR zenziwe ngeplastiki, ebangela ukuba bathembeke kwaye basebenzise lula. Ukongeza, ezinye iipleyiti ze-PCR zidibene ngokukhethekileyo ukuthintela ukubopha kweemolekyuli zeDNA kunye nokuthintela ungcoliseko. Ukusetyenziswa kweepleyiti ze-PCR kubalulekile ekunciphiseni amanyathelo amakhulu emisebenzi ebezimiselwe ngaphambili kwii-microcent okanye oomatshini be-PCRCR.
I-PCR Tube:
I-PUBR Iityhubhu ezincinci, zihlala zenziwa ngePolypropylene, esetyenziselwa ukubamba umxube wokuphendula we-PCR ngexesha le-Amlphation. Baza ngemibala eyahlukeneyo, kodwa eyona ixhaphakileyo icacile kwaye iguqukile. Chicile i-pubc tubes zihlala zisetyenziswa xa abasebenzisi bafuna ukujonga i-DNA eluntwini kuba babonakala.
Ezi tyhubhu zenzelwe ukumelana namaqondo obushushu aphezulu kunye noxinzelelo olufumaneka kuomatshini be-PCRR, bebenza balungele ubuvavanyo ye-PCR. Ukongeza kwi-amubrication, ii-fubrus ze-PCR zinokusetyenziselwa ezinye izicelo ezinje ngokulandelelana kwe-DNA kunye nokucocwa kunye nohlalutyo lweqhekeza.
Ukutywina ifilimu:
Ifilimu yokutywina yifilimu yeplasitiki edityaniswe kwiplate ye-PCR okanye ityhubhu yokuthintela ukuvela kunye nokungcoliseka komxube wokuphendula ngexesha le-PCR. Iifilimu zokutywina zibaluleke kakhulu kwiimvavanyo ze-PCR, njengoko i-umxube yokuphendula evulekileyo okanye naluphi na ungcoliseko lwendalo kwiplate lunokuthoba ubunyani kunye nokusebenza kwempumelelo.
Yenziwe ngePolythylene okanye iPolypropylene, kuxhomekeke kwisicelo, ezi filimu zeplastiki zixhaphake kakhulu kwaye zizenzekelayo. Ezinye iifilimu zisikwe ngaphambi kwepleyiti ye-PCR kunye neetyhubhu, ngelixa ezinye zingena kwiipleyi kwaye zinokusetyenziswa ngeendlela ezahlukeneyo ze-PCR okanye iityhubhu.
Iingcebiso zePipette:
Iingcebiso zepayitha zibalulekile kwiimvavanyo ze-PCR, njengoko zisetyenziselwa ukudlulisela izixa ezincinci zolwelo, ezinjengeesampulu okanye iirejista. Zihlala zenziwa nge-polyethylene kwaye zinokubamba imiqulu engamanzi ukusuka kwi-0.1 μl ukuya kwi-10 ml. Iingcebiso zepayitha ziyalahlwa kwaye zenzelwe ukusetyenziswa kancinci.
Zimbini iintlobo zeengcebiso zepayiphu-zityhafile kwaye zingafakwanga. Iingcebiso zokucoca zilungele ukuthintela nayiphi na i-aerosol okanye i-roumonity ingcoliseko, ngelixa iingcebiso ezingafakwanga zisetyenziselwa iimvavanyo ze-PCR ezisetyenziselwa izinyibilikisi ze-PRCROS okanye izisombululo ze-caustic.
Kwisishwankathelo, ipleyiti ye-PCR, i-PCR Tubis, i-membranes, kunye neengcebiso zepayitha zezinye zezicelo ezisisiseko ezifunekayo ukuze zivavanywe kakuhle. Ngokuqinisekisa ukuba unazo zonke izinto eziyimfuneko, ungangcono wenze i-PCR yovavanyo lwe-PCR ngokufanelekileyo nangokuchaneka okufunekayo. Ke ngoko, qhubeka uqinisekile ukuba unokwanela kwezi zinto zininzi ezinokufumaneka ngokulula kulo naluphi na uvavanyo lwe-PCR.
At Suzhou ace biomedical, sizinikezele ekunikezeleni ngeyona lambe iphambili kwi-lab ephezulu kuzo zonke iimfuno zakho zesayensi. Uluhlu lwethu lweIingcebiso zePipette, Ipleyiti ye-pcr, I-PUBR TUBES, kwayeUkutywina ifilimuziyilwe kakuhle kwaye zenzelwe ukuqinisekisa ukuchaneka kunye nokuchaneka kuzo zonke iimvavanyo zakho. Iingcebiso zethu zepayiphu zihambelana nazo zonke iimpawu ezinkulu zemibhobho kwaye zize kwisayizi eyahlukeneyo ukulungiselela iimfuno zakho ezithile. Iipleyiti zethu ze-PCR kunye neetyhubhu zenziwa kwizinto eziphezulu eziphezulu kwaye zenzelwe ukumelana nemijikelezo emininzi ye-thermal ngelixa igcina ingqibelelo yesampulu. Ifilimu yethu yokutywina inika itywina eliqinileyo ukuthintela ukuphuma kunye nokungcoliseka kwezinto ngaphandle. Siyakuqonda ukubaluleka kweLebhu ethembekileyo nefanelekileyo, yiyo ukuba kutheni sizabalazela ukukunika ezona mveliso kunye neenkonzo ezilungileyo zinokwenzeka. Iqela lethu leengcali lihlala likhona ukukunceda kuyo nayiphi na imibuzo okanye iinkxalabo onokuba nazo.
Ixesha lokuposa: I-08-2023