Kuphando lwemfuzo kunye namayeza, i-polymerase chain reaction (PCR) bubuchule obusetyenziswa ngokuqhelekileyo ekwandiseni iisampulu ze-DNA kwizilingo ezahlukeneyo. Le nkqubo ixhomekeke kakhulu kwizinto ezityiwayo ze-PCR eziyimfuneko kuvavanyo oluyimpumelelo. Kweli nqaku, sixoxa ngezinto ezityiwayo ezibalulekileyo zovavanyo olubanzi lwePCR: iipleyiti zePCR, iityhubhu zePCR, iimbrane zokutywina, kunye neengcebiso zepipette.
Ipleyiti yePCR:
Iipleyiti zePCR yenye yezona zinto zisetyenziswayo zibaluleke kakhulu kulo naluphi na uvavanyo lwePCR. Ziyilelwe ukukhwela ubushushu obukhawulezayo bebhayisikile kwaye zibonelela ngokugqithiselwa kobushushu obufanayo ngaphakathi kwebhore ukuze kube lula ukuphatha. Iipleyiti zifumaneka kwiindidi ezahlukeneyo zokucwangciswa kubandakanya i-96-well, 384-well, kunye ne-1536-well.
Iipleyiti zePCR zenziwe ngeplastiki, ezenza zithembeke kwaye zilula ukuphatha. Ukongeza, ezinye iipleyiti ze-PCR zigqunywe ngokukodwa ukuthintela ukubotshwa kwee-athomu ze-DNA kunye nokuthintela ukungcoliseka. Ukusetyenziswa kweepleyiti ze-PCR kubaluleke kakhulu ekunciphiseni amanyathelo omsebenzi owenziwe ngaphambili kwi-microcentrifuges okanye koomatshini be-PCR.
ityhubhu yePCR:
Iityhubhu zePCR ziityhubhu ezincinci, ezidla ngokwenziwa ngepolypropylene, ezisetyenziselwa ukubamba umxube wokusabela kwePCR ngexesha lokukhulisa. Ziza ngemibala eyahlukeneyo, kodwa ezona ziqhelekileyo zicacile kwaye ziguquguquka. Iityhubhu ze-PCR ezicacileyo zihlala zisetyenziswa xa abasebenzisi befuna ukujonga i-DNA eyandisiweyo kuba iyabonakala.
Le mibhobho yenzelwe ukumelana nobushushu obuphezulu kunye noxinzelelo olufumaneka koomatshini bePCR, bebenza ukuba balungele imifuniselo yePCR. Ukongeza kwi-amplification, ii-tubes ze-PCR zingasetyenziselwa ezinye izicelo ezifana nokulandelelana kwe-DNA kunye nokuhlanjululwa kunye nohlalutyo lwamaqhekeza.
Ifilimu yokutywina:
Ifilimu yetywina yifilimu yeplastiki encamathelayo encanyathiselwe phezulu kwipleyiti yePCR okanye ityhubhu ukuthintela ukuvela komphunga kunye nokungcoliseka komxube wokusabela ngexesha lePCR. Iifilimu zokutywina zibaluleke kakhulu kuvavanyo lwe-PCR, njengoko imixube yokusabela eveziweyo okanye nakuphi na ukungcoliseka kwendalo kwipleyiti kunokubeka esichengeni ukunyaniseka nokusebenza kovavanyo.
Yenziwe nge-polyethylene okanye i-polypropylene, kuxhomekeke kwisicelo, ezi filimu zeplastiki zinokumelana nokushisa kakhulu kwaye ziyakwazi ukuzimela. Ezinye iifilimu zisikwe kwangaphambili kwiipleyiti ze-PCR kunye neetyhubhu, ngelixa ezinye zingena kwimiqulu kwaye zingasetyenziswa ngeendidi ze-PCR okanye iibhubhu.
Iingcebiso zePipette:
Iingcebiso zePipette ziyimfuneko ekusetyenzisweni kwimifuniselo ye-PCR, njengoko zisetyenziselwa ukuhambisa izixa ezincinci zolwelo, ezifana neesampulu okanye ii-reagents. Ngokuqhelekileyo zenziwe nge-polyethylene kwaye zinokubamba umthamo wolwelo ukusuka kwi-0.1 µL ukuya kwi-10 mL. Iingcebiso zePipette ziyalahlwa kwaye zenzelwe ukusetyenziswa kanye kuphela.
Kukho iintlobo ezimbini zeengcebiso zepipette - ezihluziweyo kunye nezingahluzwanga. Iingcebiso zesihluzi zifanelekile ukuthintela nayiphi na i-aerosol okanye i-droplet contamination ukuba ingenzeki, ngelixa iingcebiso ezingahluziyo zisetyenziselwa imifuniselo ye-PCR kusetyenziswa izinyibilikisi ze-inorganic okanye izisombululo ze-caustic.
Isishwankathelo, iipleyiti ze-PCR, iityhubhu ze-PCR, i-membrane zokutywina, kunye neengcebiso ze-pipette zezinye zezinto ezisisiseko ezifunekayo kuvavanyo olubanzi lwe-PCR. Ngokuqinisekisa ukuba unazo zonke izinto eziyimfuneko, unokwenza ngcono imifuniselo ye-PCR ngokufanelekileyo nangokuchaneka okudingayo. Ke ngoko, hlala uqinisekisa ukuba unokwaneleyo kwezi zinto zisetyenziswayo zifumaneka lula kulo naluphi na uvavanyo lwePCR.
At Suzhou Ace Biomedical, sizinikele ekukuboneleleni ngezona zinto zisemgangathweni ophezulu welebhu kuzo zonke iimfuno zakho zenzululwazi. Uluhlu lwethu lweiingcebiso zepipette, Iipleyiti zePCR, Iityhubhu zePCR, kwayeifilimu yokutywinaziyilwe ngobuchule kwaye ziyilwe ukuqinisekisa ukuchaneka nokuchaneka kuyo yonke imifuniselo yakho. Iingcebiso zethu ze-pipette zihambelana nazo zonke iimpawu ezinkulu ze-pipettes kwaye ziza ngobukhulu obuhlukeneyo ukuze zilungele iimfuno zakho ezithile. Iipleyiti zethu zePCR kunye neetyhubhu zenziwe ngezinto ezikumgangatho ophezulu kwaye ziyilelwe ukuba zimelane nemijikelo emininzi ye-thermal ngelixa igcina ingqibelelo yesampulu. Ifilimu yethu yokutywina ibonelela ngesitywina esiqinileyo ukuthintela ukuvela komphunga kunye nokungcoliswa kwezinto zangaphandle. Siyakuqonda ukubaluleka konikezelo lwaselebhu oluthembekileyo nolusebenzayo, yiyo loo nto sizama ukukunika ezona mveliso kunye neenkonzo ezingcono kangangoko sinakho. Iqela lethu leengcaphephe lihlala likhona ukukunceda ngayo nayiphi na imibuzo okanye izinto ezikuxhalabisayo onokuba nazo.
Ixesha lokuposa: May-08-2023