Ngaba uyinqumle iincam yepipettexa ufaka i-glycerol? Ndenze ngexesha lam le-PhD, kodwa kwafuneka ndifunde ukuba oku kwandisa ukuchaneka kunye nokungachaneki kwepayipi yam. Kwaye ukunyaniseka xa ndisike incam, ngendandigalele ngokuthe ngqo i-glycerol esuka ebhotileni kwityhubhu. Ke ndiye ndatshintsha ubuchule bam bokuphucula iziphumo zemibhobho kwaye ndifumane iziphumo ezithembekileyo nezinokuphinda zenziwe kwakhona xa ndisebenza ngolwelo lwe-viscous.
Udidi lolwelo olufuna ingqwalasela eyodwa xa imibhobho iyilwelo lwe-viscous. Ezi zisoloko zisetyenziswa kwilebhu, nokuba zikwimo esulungekileyo okanye njengezixhobo zesithinteli. Abameli abadumileyo be-viscous liquids kwii-laboratories zophando yi-glycerol, i-Triton X-100 kunye ne-Tween® 20. Kodwa kwakhona, iilebhu ezenza ukulawulwa komgangatho wokutya, izimonyo, i-pharmaceuticals kunye nezinye iimveliso zabathengi zijongene nezisombululo ze-viscous imihla ngemihla.
I-viscosity ichazwa njenge-dynamic, okanye i-kinematic viscosity. Kweli nqaku ndigxininisa kwi-viscosity eguquguqukayo yolwelo njengoko ichaza intshukumo yolwelo. Iqondo le-viscosity lichazwe kwi-millipascal ngesekhondi (mPa * s). Endaweni yoko iisampuli zolwelo ezijikeleze i-200 mPa*s ezifana ne-85 % glycerol zisenokudluliselwa kusetyenziswa i-pipette ye-air-cushion yakudala. Xa usebenzisa ubuchule obukhethekileyo, i-reverse pipetting, i-aspiration of air bubbles okanye i-residues kwi-tip iyancipha kakhulu kwaye ikhokelela kwiziphumo ezichanekileyo zepayipi. Kodwa kunjalo, ayisiyiyo eyona nto sinokuyenza ukuphucula imibhobho yolwelo lwe-viscous (jonga umzobo 1).
Xa i-viscosity inyuka, ubunzima buyanda. Izisombululo ze-viscous eziphakathi ukuya kuthi ga kwi-1,000 mPa*s kunzima kakhulu ukuhanjiswa kusetyenziswa iipipette ze-classic air-cushion. Ngenxa yokukhuhlana okukhulu kwangaphakathi kweeathom, ulwelo lwe-viscous lunokuziphatha okucothayo kakhulu kwaye imibhobho kufuneka yenziwe ngokucothayo nangononophelo. Ubuchule bokuReverse Pipetting kaninzi abanelanga kugqithiso oluchanekileyo lolwelo kwaye abantu abaninzi balinganisela iisampulu zabo. Esi sicwangciso sikwathetha ukuthathela ingqalelo ukuxinana kolwelo kwakunye neemeko zaselabhoratri ezifana nokufuma kunye nobushushu ukubala ngokuchanekileyo umthamo wolwelo ofunekayo kubunzima. Ke ngoko, ezinye izixhobo zombhobho, ezibizwa ngokuba zizixhobo ezifanelekileyo zokufuduka, ziyacetyiswa. Ezi zinencam enepiston edibeneyo, njengesirinji. Ke ngoko, ulwelo lunokunqweneleka ngokulula kwaye lukhutshwe ngelixa ukuhanjiswa kolwelo okuchanekileyo kunikezelwa. Ubuchule obukhethekileyo abuyimfuneko.
Nangona kunjalo, izixhobo ezifanelekileyo zokufuduka zifikelela kumda onezisombululo ezibonakala kakhulu ezinje ngolwelo lobusi, ikhrimu yesikhumba okanye ioyile ethile yoomatshini. Olulwelo lufuna kakhulu lufuna esinye isixhobo esikhethekileyo esikwasebenzisa umgaqo-siseko wokufuduka kodwa ukongezelela sinoyilo olulungisiweyo lokujongana nezisombululo ezibonakala kakhulu. Esi sixhobo esikhethekileyo siye safaniswa neengcebiso ezikhoyo zokufuduka ezifanelekileyo ukuze kufumaneke umda apho kubalulekile ukutshintshela kwi-tip eqhelekileyo yokusabalalisa ukuya kwingcebiso ekhethekileyo yezisombululo ezibukhali kakhulu. Kwaboniswa ukuba ukuchaneka kunyuswa kwaye imikhosi efunekayo yokulangazelela kunye nokukhupha iyancitshiswa xa usebenzisa ingcebiso ekhethekileyo yolwelo olubonakalayo. Ngolwazi oluthe vetshe kunye nemizekelo elulwelo, nceda ukhuphele i-Applicaton Note 376 ekusebenzeni okulungiselelweyo kulwelo olubonakalayo.
Ixesha lokuposa: Jan-23-2023