Ukuqala umfuniselo kuthetha ukubuza imibuzo emininzi. Ziziphi izinto ezifunekayo? Zeziphi iisampuli ezisetyenziswayo? Zeziphi iimeko eziyimfuneko, umzekelo, ukukhula? Side kangakanani isicelo siphela? Ngaba kufuneka ndijonge ulingo ngeempelaveki, okanye ebusuku? Umbuzo omnye udla ngokulityalwa, kodwa awubalulekanga kangako. Loluphi ulwelo olusetyenziswayo ngexesha lokufakwa kwesicelo kwaye lufakwa njani ngemibhobho?
Kuba ulwelo lwemibhobho lishishini lemihla ngemihla kwaye ukuba ulwelo olunqwenelekayo luyanikezelwa, asidli ngokuchitha ixesha elininzi kunye nomzamo kwesi sihloko. Kodwa kunengqiqo ukucinga kabini malunga nesixhobo se-liquid kunye ne-pipette esetyenzisiweyo.
Ulwelo lunokwahlulwa ngokweendidi ezintlanu eziphambili: ezinamanzi, i-viscous (kubandakanywa nezicoci), eziguquguqukayo, ezixineneyo kunye nezosulelayo okanye ezinetyhefu. Ukuphathwa ngendlela engafanelekanga kwezi ndidi zolwelo kunempembelelo enkulu kwisiphumo semibhobho. Ngelixa imibhobho yezisombululo ezinamanzi njengezithinteli ezininzi zilula ngokufanelekileyo kwaye zenziwa ikakhulu ngeepipettes zakudala zombhobho womoya, ubunzima busenokuvela xa kufakwe imibhobho engamanzi aguquguqukayo njengeacetone. Ulwelo oluguquguqukayo lunoxinzelelo oluphezulu lomphunga olubangela ukuba umoya ube ngumphunga kwi-cushion yomoya kwaye ngokwenza njalo ukwakheka kwamathontsi. Ekugqibeleni, oku kuthetha isampuli okanye ilahleko ye-reagent ngaphandle kobuchule obuchanekileyo bombhobho. Xa ukubhobhoza ulwelo oluguquguqukayo, ukumanzisa kwangaphambili kweincam yepipette(ukulangazelela okuphindaphindiweyo kunye nemijikelo yokukhupha ukufumisa umoya ngaphakathi kwincam) kunyanzelekile ukunyusa ukuchaneka kwemibhobho. Udidi lwelwelo olwahluke ngokupheleleyo lubandakanya ulwelo lwe-viscous olufana ne-glycerol. Ezi zinendlela yokuziphatha ecothayo kakhulu ngenxa yokukhuhlana okukhulu kwangaphakathi kweeathom ezikhokelela kumnqweno weqamza lomoya, iintsalela kwincam kunye nesampulu okanye ilahleko yereagent. Ubuchwephesha obukhethekileyo bokubhobhoza obubizwa ngokuba yi-reverse pipetting iyanconywa xa usebenzisa i-classic air-cushion pipettes. Kodwa eyona nto ingcono kukusetyenziswa kwesixhobo esahlukileyo sombhobho, isixhobo sokufuduka esinencam efana nesirinji esebenza ngaphandle komqamelo womoya phakathi kwesampulu kunye nepiston ngaphakathi kwincam. Ulwelo lunokunqweneleka ngokukhawuleza kwaye lula ngezi zixhobo. Xa uhambisa i-viscous liquid, umthamo opheleleyo unokuchithwa ngaphandle kwee-residus kwi-tip.
Ke, ukucinga malunga nolwelo ngaphambi kokuba uqalise umfuniselo kunokwenza lula kwaye kuphuculwe ukuhamba kwakho komsebenzi kunye neziphumo. Uqwalaselo lweendidi ze-liquid, imingeni yabo kunye neengcebiso malunga nobuchule obufanelekileyo bombhobho kunye nezixhobo zokubhobhoza ziboniswa kwipowusta yethu. Usenokukhuphela ipowusta ukuze ube noguqulelo oluprintwayo kwilabhu yakho.
ISuzhou ACE Biomedical Technology Co., Ltd yinkampani yobuchwephesha ezinikele ekuboneleleni ngezinto ezilahlwayo ezikumgangatho ophezulu zonyango kunye nelebhu yeplastiki esetyenziswa ezibhedlele, kwiikliniki, kwiilebhu zokuxilonga kunye neelebhu zophando lwesayensi yobomi. Sinoluhlu lweIingcebiso ze-pipette (Iingcebiso zendalo yonke, iingcebiso ezizenzekelayo), microplate(24,48,96 amaqula), PCR izinto ezisetyenziswayo(ipleyiti yePCR, iityhubhu, iifilimu zokutywina),Umbhobho weCryovialnjalo njalo, sinokubonelela ngenkonzo ye-OEM/ODM, wamkelekile ukuba uqhagamshelane nathi ukuba unayo naziphi na iimfuno.
Suzhou ACE Biomedical Technology Co.,Ltd
I-imeyile:Joeyren@ace-biomedical.com
Umnxeba:+86 18912386807
Iwebhusayithi:www.ace-biomedical.com
Ixesha lokuposa: Feb-09-2023