I-polymerase chain reaction (PCR) yindlela esetyenziswa ngokubanzi ngabaphandi bebhayoloji, inzululwazi yezobungcwalisa kunye neengcali zelabhoratri zonyango.
Ukubala ezimbalwa zezicelo zayo, isetyenziselwa i-genotyping, ulandelelwano, i-cloning, kunye nohlalutyo lwe-gene expression.
Nangona kunjalo, ukuleyibhela iityhubhu zePCR kunzima kuba zincinci kwaye zinendawo encinci yokugcina ulwazi.
Ngelixa, iipleyiti zobungakanani be-PCR (qPCR) zibhalwe kuphela kwicala elinye.
Ngaba udinga isigxina, esiqinileyo ityhubhu yePCRukuba isetyenziswe kwilabhoratri yakho? Zama ukuxhasa umenzi owaziwayo.
Iphakheji Yonke
I-PCR-Tag Trax elinde ilungelo elilodwa lomenzi wechiza lolona khetho lwamva nje kunye nolona lungcono lokulebhelisha iityhubhu zePCR ezikwiprofayili ephezulu, imicu, kunye neeplats ze-qPCR.
Uyilo oluguquguqukayo lwethegi ye-non-adhesive yenza ukuba ikwazi ukuchonga i-0.2 ml yeprofayili ephezulu yeetyhubhu ze-PCR kunye neepleyiti ze-qPCR ezingagqokwanga kwiinkqubo ezahlukeneyo.
Inzuzo ephambili ye-PCR-Tag Trax kukukwazi ukubonelela ngeyona ndawo ifanelekileyo yendawo yokushicilela okanye, ukuba kuyimfuneko, ukubhala ngesandla.
Ukusebenzisa umshicileli wokudlulisa i-thermal, iithegi zinokuprintwa ngenombolo ezilandelelanisiweyo kunye ne-1D okanye i-2D yebhakhowudi kwaye inokumelana namaqondo obushushu asezantsi njenge -196 ° C kwaye aphezulu njenge +150 ° C.
Oku kubenza bahambelane kunye neebhayisekile ezininzi ze-thermo. Luluvo olulungileyo ukuvavanya isampulu yeethegi kwiibhayisekile zakho ze-thermo ukuqinisekisa ukuba aziphazamisi iimpendulo.
Kufuneka babe ngabahlobo beglavu, babonelele ngeliso lentaka ngokukhawuleza ulwazi olubhalwe kwiithegi emva kokuba iibhayisekile ze-thermo zivuliwe.
Iityhubhu zePCR zinokuza ngemibala eyahlukeneyo okanye ifomathi enemibala emininzi ukuze kube lula ukuleyibhelishwa kombala.
Iithegi ezincamathelayo zingasetyenziswa njengenkxaso yeetyhubhu zakho, zenza kube lula ukufaka ii-reagents kwi-pipette kuzo kwaye uzigcine efrijini okanye kwisikhenkcisi emva kokusabela.
Iityhubhu zePCR, 0.2mL
Iityhubhu zePCR zomntu ngamnye zinokubhalwa kwiindawo ezimbini ezahlukeneyo: iityhubhu kunye nekepusi yayo.
Ukufakwa kwekhowudi yombala lula, iilebhile ezisecaleni zetyhubhu zePCR ezincinci ziyafumaneka ngenani lemibala yazo zombini iiprinta zelaser kunye ne-thermal-transfer.
Ulwazi oluninzi lunokuprintwa kwezi zilebhile zetyhubhu zePCR kunokuba zibhalwe ngesandla, kwaye iibhakhowudi zingasetyenziselwa ukuphucula ukulandeleka.
Iileyibhile zikhuselekile kwaye zinokugcinwa kwizikhenkcisi zaselebhu kangangexesha elide.
Iileyibhile zamachaphaza angqukuva lolona khetho lulungileyo lokuleyibhela imiphezulu yetyhubhu yePCR.
Iileyibhile zamachaphaza, kwelinye icala, zinenani elilinganiselweyo lendawo kwityhubhu yokuprinta okanye ukubhala ulwazi. Ke ngoko ukubenza ukuba ibe yenye yezona ndlela zincinci zokuleyibhela iityhubhu zePCR.
Ukuba kufuneka usebenzise iileyibhile zamachaphaza kwiityhubhu ze-PCR kwaye uya kubhala inani elikhulu lazo, i-pikaTAG TM.
I-pikaTAG TM sisixhobo sesicelo esithatha iileyibhile zamachaphaza ngokuthe ngqo kwi-liner yazo kwaye zinamathele phezulu kwiibhubhu.
Iqhayisa nge-ergonomic pen-like form eyenza ukuleyibheli kwechaphaza ngokukhawuleza kwaye kulula, ukususa umsebenzi ochitha ixesha lokuchola iilebhile ezincinci kunye nokuthintela ukulimala koxinzelelo olubangelwa yi-tube label.
Imicu yeeMibhobho zePCR
Imicu ye-PCR ihlala isetyenziswa kwiilebhu ezisebenzisa iinkqubo ezininzi ze-PCR kunye ne-qPCR.
Ukuleyibhelishwa kwezi migca kulucelomngeni ngakumbi kunokuleyibhelisela iityhubhu nganye kuba ityhubhu nganye iqhagamshelwe kwelandelayo, ngokwenjenjalo kuncitshiswa indawo esele ithintelwe.
Ngethamsanqa, imicu yelebhile ye-8-tube ihambelana ityhubhu nganye, isenza umcu wePCR ubhale impepho.
Ezi ziqwenga ziyilwe yi-GA ngamazwe, zinemingxuma phakathi kweleyibhile nganye ekuluhlu, ekuvumela ukuba uprinte iilebhile ezininzi njengoko kukho iityhubhu.
Beka umgca weleyibhile yonke ecaleni kwecala lombhobho, unamathisele zonke iileyibhile ngexesha elifanayo, uze uphule i-perforations ukuze ugcine iilebhile zifakwe ngokuqinileyo kwicala.
Kuluhlu lobushushu obuphakathi kwe -80 ° C ukuya + 100 ° C, ezi layibhile eziprintwayo zokudluliselwa kwe-thermal zikhuselekile ukuba zisetyenziswe kwiibhayisekile ze-thermo kwaye zinokugcinwa ngokukhuselekileyo kwizikhenkcisi zaselabhoratri.
Indlela yesiNtu
Ukubhala ngesandla yeyona ndlela ixhaphakileyo yokuchonga iityhubhu zePCR, nangona ikude lee kokulungileyo kuba ukubhala ngokucacileyo kwiityhubhu zePCR akunakwenzeka.
Ukubhala ngesandla kuphelisa uthotho kunye neebarcodes, nto leyo eyenza kube nzima ngakumbi ukulandelela iisampuli zakho.
Ukuba ukubhala kuphela kokhetho kwilebhu yakho, iziphawuli ze-cryo ezinencam entle zifanelekile ukuba utyale imali kuba zikuvumela ukuba ubhale ngokufundekayo kangangoko ngaphandle kokucima okanye ukufiphaza.
Qhagamshelana nathi ngeMibhobho yePCR ekumgangatho ophezulu
Senza kwaye sivelise umgangatho ophezuluIityhubhu zePCRukuze zisetyenziswe kwi-genotyping, ulandelelwano, i-cloning, kunye nohlalutyo lwejeni kwiilabhoratri zonyango ezahlukeneyo kunye neziko lophando.
Ngowona mava angcono ngeetyhubhu zePCR, yenzafikelele kuthi ngemveliso esemgangathweni nesebenzayo.
Ixesha lokuposa: Oct-30-2021