I-criovialszisetyenziselwa ukugcinwa kwe-Cryzogesic yemigca yeseli kunye nezinye izixhobo zebhayiloji ezigxekayo, kwii-Dewors ezizaliswe yinitrogen yolwelo.

Kukho amanqanaba aliqela abandakanyekayo kulondolozo oluphumelelayo lweeseli kwi-nitrogen yolwelo. Ngelixa umgaqo osisiseko kukuhamba kancinci, eyona ndlela iqashwe ngqo ixhomekeke kwiseli yeseli kunye ne-cryoprotecttent. Kukho izinto ezininzi zokhuseleko kunye neendlela ezilungileyo zokuthatha ingqalelo xa zigcina iiseli ezinamaqondo obushushu asezantsi.

Esi sithuba sijolise ekunikezeleni isishwankathelo sendlela i-lriovial igcinwe njani kwi-witrogen yolwelo.

Zeziphi ii-criovials

Ii-criovials zincinci, ii-vials ezikhawulezayo ezenzelwe ukugcina iisampulu zerediyo kumaqondo obushushu asezantsi kakhulu. Baqinisekisa ukuba iiseli ezigcinwe kwi-Crytoprotectant azingeni ngqo kwi-nitrogen yolwelo, zinciphisa umngcipheko weziphumo zeselula ngelixa zisaxhamla kwisiphumo sokupholisa initrogen.

Ii-vials zihlala zifumaneka kuluhlu lwevolumu kunye noyilo-banokuntywila ngaphakathi okanye ngaphandle kwentambo ngaphakathi okanye ngaphandle kwebhotile. Iifomati ezingenamkhethe kunye nezingacocekanga ziyafumaneka.

Ngubani osebenzisaI-cyrovialsukugcina iiseli kwi-nitrogen ye-nitrogen

Uluhlu lweelebhu ze-NHS kunye nelebhu yabucala, kunye namaziko ophando akhangela kwi-Biology yeTyhini, i-bitologal yeseli, i-biology kunye ne-biology yeseli esebenzisa i-cryovial

Iiseli ezigcinwe ngale ndlela zibandakanya iiseli ze-B kunye neeseli, iiseli ze-CHEMATOET kunye neeseli ze-hematoomboe, iiseli ze-proine, i-ronorinal, iiseli ze-ronomal, iiseli ze-ceripouter.

Amagqabantshintshi ngendlela yokugcina i-crieovials kwi-nitrogen ye-nitrogen

I-Crytoopresetion yinkqubo egcina iiseli kunye nezinye izinto zebhayiloji zebhayiloji ngokukholisa kubushushu obuphantsi kakhulu. Iiseli zinokugcinwa kwi-nitrogen yolwelo kangangeminyaka ngaphandle kwelahleko yokusebenza kweseli. Olu luhlu lweenkqubo ezisetyenzisiweyo.

Ukulungiswa kweseli

Eyona ndlela yokulungiselela iisampulu ziya kwahluka ngokuxhomekeka kuhlobo lweseli, kodwa ngokubanzi, iiseli ziqokelelwa kwaye zifakwe i-cent-cellet enesisu esityebileyo. Le pellet iphinda iphinde igcinwe kwi-superntunt exutywe ne-cryprotectant okanye i-cryopreation.

Iphakathi

I-Medium iqeshelwe ukugcina iiseli kwiindawo ezineqondo lobushushu eziphantsi ziya kuphangwa ngokuthintelwa i-Intra kunye neekristali zekristali kunye neseli ekufeni. Indima yabo kukubonelela ngendawo ekhuselekileyo, yokukhusela kwiseli kunye nezicubu ngexesha lokukhulula, ukugcinwa, kunye neenkqubo zokutsha.

Iphakathi elifana neplasma entsha ye-frozen (FFP), isisombululo sePlasmalyte okanye isisombululo se-Stuum-simahla, izisombululo zasimahla ze-SUMIS-SIP ezixutywe ne-dimthyl sulphoxide (DMSO) okanye i-vlycerol.

I-pellet yesampuli enokuphinda isuswe i-pellet ifakwe kwi-polyproplials i-rieovials efana neI-Suzhou Ace Ace Inkampani ye-Cryogenic Stores.

Kubalulekile ukuba ungayiphembeli i-cleovials njengoko oku kuyakwandisa umngcipheko wokuqhekeka kwaye ukukhululwa okukhoyo (1).

Ixabiso elilawulwayo

Ngokubanzi, izinga le-freeze elisezantsi le-freeze liqeshelwe ukuthungwa ngempumelelo kweeseli.

Emva kweesampulu zangenyeziwe kwi-Cryogenic Vials, zibekwe kwi-ices emanzi okanye kwifriji kunye nenkqubo yokukhuphela kuqalwa ngaphakathi kwemizuzu emi-5. Njengesikhokelo ngokubanzi, iiseli zipholile ngenqanaba le -1 ukuya -3 ngomzuzu (2). Oku kufezekiswa kusetyenziswa i-Compleler okanye ngokubeka i-vials kwibhokisi engafakwanga kwi-A-7 ° C ukuya kwi-CARMERE.

Dlulisela kwi-nitrogen yolwelo

I-vials ye-cryzogen crymogenic idluliselwe kwitanki ye-nitrogen ye-nitrogen yexesha elingenasiphelo linike iqondo lobushushu elingaphantsi kwe-135 ℃ ligcinwa.

La maqondo obushushu e-Ultra anokufumaneka ngokuntywiliselwa kwintuthuzelo kwi-fluid okanye i-vapor witrogen.

ISigaba se-Liqual okanye i-Vapor?

Ukugcinwa kwiSigaba seSigaba seSigaba soLwandle siyaziwa ukugcina ubushushu obubandayo ngokuhambelana ngokupheleleyo, kodwa kuhlala kungakhuthazwa ngenxa yezi zizathu zilandelayo:

• Imfuneko yevolumu enkulu (ubunzulu) ye-nitrogen yolwelo oluyingozi. Ukutshisa okanye i-asphyxiation ngenxa yoku ngumngcipheko wokwenyani.
• Amatyala abhaliweyo okungcoliseka komnqamlezo ngokungcoliseka kwe-esferious efana ne-Aspergilsus, i-HEP B kunye ne-Viprage kusasa nge-nitrogen ephakathi (2,3)
• Inokubakho kwi-nitrogen yolwelo ukuba ivule kwi-vials ngexesha lokuntywila. Xa isuswe kwindawo yokugcina kwaye ifudumeza kubushushu begumbi, i-nitrogen ngokukhawuleza iyakhula. Ngenxa yoko, i-vial inokuthi itshabalalise xa isuswe kwindawo yokugcina i-nitrogen ye-nitrogen, eyenza ingozi evela kuzo zombini iimpukane zokubhabha kunye nokuvezwa kwesiqulatho (1, 4).

Ngezi zizathu, ukugcinwa kobushushu obuninzi kubushushu kuxhaphake kakhulu kwiSigaba se-vapor nitrogen. Xa iisampulu kufuneka zigcinwe kwiSigaba soLwandle, i-tublex ye-tuby ekhethekileyo kufuneka isetyenziswe.

I-Dowden kwisigaba se-vapor kukuba iqondo lobushushu elithe nkqo linokwenzeka kukhokelela kwiqondo lokuhamba kwamaqondo obushushu phakathi kwe-135 ℃ kunye -190 ℃. Oku kunyanzelisa ukubekwa kweliso ngononophelo nangokukhuthala kwamanqanaba e-nitrogen ye-nitrogen kunye neqondo lokushisa (5).

Abavelisi abaninzi bacebisa ukuba ii-criovials zilungele ukugcina phantsi ukuya kwi-1: 35 ℃ okanye ukusetyenziswa kwinqanaba le-vapor kuphela.

Ukutsalwa kweeseli zakho ezigciniweyo

Inkqubo yokutsala ixinezelekile kwinkcubeko ye-qadi, kwaye ukuphatha ngokufanelekileyo kwaye ubuchule buyacelwa ukuqinisekisa ukusebenza kakuhle, ukubuyisela kwakhona kunye nokusebenza kweeseli. Iiprotokholi ngqo ngqo ziya kuxhomekeka kwiindidi ezithile zeseli. Nangona kunjalo, ukukhawuleza okukhawulezayo kuthathwa njengomgangatho ku:

• Nciphisa naliphi na ifuthe lokufumana kwakhona i-selfowuni
• Nceda ukunciphisa ixesha lokuvezwa kwiiluts ezikhoyo kwimithombo yeendaba
• Nciphisa nawuphi na umonakalo ngumkhenkce

Iibhafu zamanzi, iibhafu zeBead, okanye izixhobo ezizodwa ezenzekelayo ziqhele ukusetyenziswa kwiisampulu.

Uninzi rhoqo lweseli luyekwe ngexesha lemizuzu emi-1-2, ngokucheba kancinci kwi-37 ℃ i-37 ℃ ibe yindawo encinci yomkhenkce kwi-vial ngaphambi kokuba ihlanjwe kwindawo ephakathi.

Kwezinye iiseli ezinjengemibungu yaseMammalian, ukufudumala okucothayo kubalulekile ukuze basindile.

Iiseli zilungele ukwenkcinkca zeselula, indawo yeselula, okanye kwimeko yeeseli ze-haematopoiec ze-haematopoiec - izifundo zokuqinisekisa ukuthembeka kweeseli ze-Sgranotive.

Yinto eqhelekileyo ukuthatha i-alquets encinci yesampulu eyakhelwe kwangaphambili esetyenziselwa ukwenza ukubalwa kweseli ukumisela ubuchule bokubekwa kwinkcubeko. Ungavavanya iziphumo zeenkqubo zokuzodwa zeselula kwaye umisele ukusebenza kweseli.

Ezona ndlela zibalulekileyo zokugcina izitolo

I-cryopresses ephumeleleyo yeesampulu ezigcinwe kwii-cleovials zixhomekeke kwizinto ezininzi ezikumthetho olandelwayo kubandakanya ukugcinwa ngokufanelekileyo nokurekhodwa.

• Yahlula iiseli phakathi kweendawo zokugcina-Ukuba ivolumu ivumela, hlula iiseli phakathi kwee-vials kwaye uzigcine kwiindawo ezahlukeneyo ukunciphisa umngcipheko welahleko yesampulu ngenxa yokusilela kwezixhobo.
• Thintela ungcoliseko olunqamlezileyo-Khetha i-vials engatshatanga i-rials okanye i-autoclave ngaphambi kokusetyenziswa okulandelayo
• Sebenzisa i-vials ye-specker ye-SPERS kwiiseli zakho-I-Vials ziza kuluhlu lwevolumu phakathi kwe-1 ne-5mls. Kulumkele ukugqobhoza i-vials ukunciphisa umngcipheko wokuqhekeka.
• Khetha i-criyogenic vials-I-vials ezixineneyo ngaphakathi ziyacetyiswa ziiyunivesithi zendlela yokhuseleko-banokuthintela ungcoliseko ngexesha lokugcwalisa okanye xa kugcinwe kwi-nitrogen yolwelo.
• Thintela ukuvuza-Fumana amatywina e-BI-i-bi-bi-bin axolwe kwi-cap-cap okanye i-o-rings ukuthintela ukuvuza kunye nongcoliseko.
• Sebenzisa i-2D Barcodes kunye nelebhile i-vials-Ukuqinisekisa ukulandelela, i-victs ezineendawo ezinkulu zokubhala zenza ukuba i-vial nganye ibe ilebhile eyaneleyo. I-2D Barcode inokunceda ngolawulo lokugcina kunye nokugcinwa kweerekhodi. I-CAPS yekhowudi yekhowudi iluncedo ekuchongeni lula.
• Isondlo esaneleyo sokugcina-Ukuqinisekisa ukuba iiseli azilahleki, iinqanawa zokugcina kufuneka zibeke iliso kubushushu kunye nenqanaba le-nitrogen engamanzi. Iialarm kufuneka zilungele ukulumkisa abasebenzisi beempazamo.

Amanyathelo okhuseleko

I-nitrogen yolwelo sele iqhelisela kuphando lwale mihla, kodwa ithwala umngcipheko wokulimala kakhulu ukuba isetyenziswe ngokungachanekanga.

Izixhobo ezifanelekileyo zokukhusela (i-PPE) kufuneka inxibe ukunciphisa umngcipheko we-rostbite, itshisa kunye nezinye izinto ezinzima xa uphatha i-nitrogen ye-nitrogen. Nxiba

• Iiglosi ze-Crygenic
• I-Laboratory Coat
• Ifuthe elixhaphakileyo yobuso obunganyangekiyo egubungela intamo
• Izihlangu ezivaliweyo
• Iplastikhi yeplastikhi yeSplashproof

Ifriji ye-nitrogen ye-nitrogen kufuneka ibekwe kwiindawo ezinomoya ezingena kakuhle ukunciphisa umngcipheko we-asphyxiation-isibaleki i-nitrogen varogen varogen vaponer kwaye inyuselwe ioksijini ye-themosphegen. Iivenkile zevolumu ezinkulu kufuneka zibe neenkqubo ze-alarm eziphantsi.

Ukusebenza ngababini xa uphatha i-nitrogen ye-witrogen ilunge kwaye ukusetyenziswa kwayo ngaphandle kweeyure zomsebenzi eziqhelekileyo kufuneka zithintelwe.

I-criovials yokuxhasa indlela ohamba ngayo umsebenzi wakho

I-Suzhou Ace Inkampani ye-biomedical ibonelela ngokukhetha ngokubanzi kweemveliso ezihlangabezana neemfuno zakho ze-cryprestion yeentlobo ezahlukeneyo zeeseli. Iphothifoliyo ibandakanya uluhlu lwee-tubeess kakuhle njengoluhlu lwee-riovials ezinqindi.

Iikrizo zethu zezi:

• I-Lab Screw cap 0.5ml 1.5ml 2.0ml 2.0ml I-2.0ml I-Cryoogesic Vials i-vials

  ● I-0.5ml, i-1.5ml, i-2.0ml Inkcazo, isiketi okanye ngaphandle kwengozi
  ● Iyilo oluyeyonayo okanye lokuzimela loyilo, i-stele okanye i-non-puse ifumaneka zombini
  ● I-screw tube zenziwe ngenqanaba lezonyango lwe-Polyproplene
  ● I-PP ye-vials i-vials inokuphinda idlule kwaye ityalwe
  ● Uyilo lwangaphandle lwe-cap lunokunciphisa ukubakho kongcoliseko ngexesha lonyango lwesampula.
  ● I-screw cap criogenic tubes tubes curws ze-screws zokusetyenziswa
  ● Iityhubhu zihambelana ne-rotors eqhelekileyo
  ● I-Cry Gebgenic Tube o-i-tube i-tubes efanelekileyo ye-1-intshi kunye ne-2-intshi, i-48WEll, i-81well, i-96well kunye neebhokisi ze-readzer zefriji
  ● I-AutochedE i-Auto ukuya kwi-121 ° C kwaye iphindwe kwi-36 ° C

  Icandelo hayi	Isixhobo	Ivolumu	CapUmbala	IiPC /Ibhegi	Iingxowa / ityala
  I-Act05-bl-n	PP	I-0.5ml	Mnyama, tyheli, iblowu, ibomvu, i-purple, mhlophe	I-500	10
  I-Act15-bl-n	PP	1.5ml	Mnyama, tyheli, iblowu, ibomvu, i-purple, mhlophe	I-500	10
  I-Act15-BL-NW	PP	1.5ml	Mnyama, tyheli, iblowu, ibomvu, i-purple, mhlophe	I-500	10
  I-ArT20-bl-n	PP	2.0ml	Mnyama, tyheli, iblowu, ibomvu, i-purple, mhlophe	I-500	10