Ukubekwa emgangathweni kwezi nkqubo kubandakanya ukumiliselwa kwazo kunye nokusekwa kwaso kunye nokulungelelaniswa, ukuvumela intsebenzo yexesha elide-ngokuzimeleyo yomsebenzisi. Ukubekwa emgangathweni kuqinisekisa iziphumo ezisemgangathweni eziphezulu, kunye nokuzala kunye nokuthelekiswa.
Injongo ye (class) prc yesizukulwana sesiphumo esinokuthenjwa nesibuyisiweyo. Kwizicelo ezithile, isivuno seImveliso yePCRichaphazelekayo. Ngeempendulo, ukhathalelo kufuneka kuthathelwe ingqalelo ukuqinisekisa ukuba iisampulu azikholwanga kwaye ukuba i-PRR Umsebenzi we-PRR Umsebenzi uhlala uzinzile. Ngokukodwa, oku kuguqula ukuncitshisela ukumiliselwa konxibelelwano olunokukhokelela kwiziphumo ezibi okanye ubuxoki okanye zithintele indlela ye-PCR yokuphendula. Ngaphaya koko, iimeko ezisabelayo kufuneka zifane nesampulu nganye nganye phakathi kwabasebenzi kwaye zinokudluliselwa ekunikezelweni emva (kwendlela efanayo). Oku kubhekisa ekuqulunqweni kwendlela yokuphendula kunye nohlobo lolawulo lobushushu kumjikelezo. Iimpazamo zabasebenzisi, kunjalo, ziya kuthintelwa kangangoko kunokwenzeka.
Apha ngezantsi, siya kubonisa imiceli mngeni ehlanganelweyo ngexesha lokulungiselela kwaye sibaleke kwi-PCR-kunye neendlela ezikhoyo ezikhoyo ngokubhekisele kwizixhobo ze-PRR.
Ukulungiswa kwempendulo
Ukusasazwa kwamacandelo okuphendula kwiinqanawa ze-PCR-SORS, okanye iipleyiti, ngokulandelelana, kubandakanya imiceli mngeni emininzi ekufuneka yoyisiwe:
Iimeko zokuphendula
Owona mzobo uchanekileyo wezinto ezizezinye zibalulekile xa ujolise kwezona meko zibalulekileyo. Ukongeza kwindlela elungileyo yokutsiba, ukukhetha isixhobo esifanelekileyo kubalulekile. I-PRR Master-Huzys rhoqo iqulethe izinto eziyandisa i-viscosity okanye i-foam. Ngexesha lenkqubo yokulima, ezi zikhokelela ekumanzini kakhulu kweIingcebiso zePipette, ngaloo ndlela kunciphisa ukuchaneka kombhobho. Ukusetyenziswa kweenkqubo zokuchithwa ngokuthe ngqo okanye iingcebiso ezizezinye zepayipu ezingaphantsi kwendawo emanzi anokuphucula ukuchaneka kunye nokuchaneka kwenkqubo yombhobho.
UTSHINTSHO
Ngexesha lenkqubo yokusasaza, i-aerosols ivelisiwe ukuba,, ukuba ivunyelwe ukuba ifike ngaphakathi kombhobho, inokungcolisa enye isampulu ngexesha lenyathelo elilandelayo lombhobho. Oku kuthintelwe ngokusebenzisa iingcebiso zecebo lokucoca okanye iinkqubo zokufuduka ngokuthe ngqo.
Izinto ezinokusetyenziswa njengeiingcebiso, iinqanawa kunye neepleyiti ezisetyenziswa kumsebenzi we-PCR kufuneka zingaqulathanga isampulu okanye zibumbe iziphumo. Oku kubandakanya iDNA, iidnases, i-rnases kunye ne-PCR ethintela, kunye nezinto ezinokuthi zifumane izinto ezibonakalayo ngexesha lokuphendula.
Impazamo yomsebenzisi
Iisampuli ezingakumbi ziqwalaselwa, ukuphakama komngcipheko kwimpazamo. Inokwenzeka ngokulula ukuba isampulu ifakwe kwinqanawa engalunganga okanye engalunganga. Lo mngcipheko unokuncitshiswa ngokumakishwa ngokulula kwamaqula. Ngokuzenzekelayo kumanyathelo okusasaza, "Into yabantu", okt, iimpazamo kunye nokwahluka okunxulumene nomsebenzisi, zincitshiswa, ngaloo ndlela bevelisa ubume bendlela encinci yokuphendula. Oku kufuna ukuba iipleyiti zokuzinza okwaneleyo kokuqeshwa kwindawo yokusebenza. I-Barcode eqhotyoshelweyo ibonelela ngomatshini owongezelelweyo, owenza lula ukulandela umkhondo kuyo kuyo yonke inkqubo.
Inkqubo ye-thermoycler
Inkqubo yesixhobo inokungqina ixesha-isebenzisa ixesha kunye nempazamo. Imijikelezo ye-PRR ye-PRRRM ye-PCRMRMR SPRORS isebenza kunye ukwenze lula le nkqubo inyathelo kwaye, okona kubaluleke kakhulu, ukuyenza ikhuselekile:
Ukusebenza ngokulula kunye nesikhokelo esilungileyo somsebenzisi sisiseko senkqubo efanelekileyo. Ukwakha kwesi siseko, ulawulo lwabasebenzisi abakhuselweyo lomsebenzisi luya kuthintela iinkqubo zakho ukuba zingaguqulwa abanye abasebenzisi. Ukuba ngabahamba abaninzi (bohlobo olufanayo) bayasebenza, kuyanceda ukuba inkqubo inokudluliselwa ngqo kwisixhobo esinye ukuya kwenye i-USB okanye unxibelelwano. Isoftware yekhompyutha yenza ukuba iinkqubo eziphambili zekhompyuter kunye nezikhuselekileyo zeenkqubo, amalungelo abasebenzisi kunye namaxwebhu kwikhompyuter.
I-PCR ibaleka
Ngexesha lokubaleka, iDNA ikhulisiwe kwinqanawa yokusabela, apho isampulu nganye kufuneka ifakwe phantsi, iimeko ezisaleleyo. Le miba ilandelayo ifanelekile kwinkqubo:
Ulawulo lobushushu
Ukuchaneka okugqibeleleyo kulawulo lobushushu kunye ne-homogeneity yebloko yebhloko sisiseko semeko yobushushu kuzo zonke iisampulu. Umgangatho ophezulu wokufudumeza kunye nezinto ezipholileyo (izinto ezipholileyo (izinto zepeltier), kunye nendlela eqhagamshelwe kwibhlokhi, zizinto ezifihlakeleyo eziya kuthi ziqwalasele ingozi yamaqondo obushushu awaziwa ngokuba ngumngcipheko owaziwayo njengo "umphetho"
Ukuthuma
Ukugxininiswa kwamacandelo okwenza izinto zokuphendula akufuneki kutshintshe ngaphezulu kwendlela yokuphendula ngenxa yokuphuma. Ngaphandle koko, kunokwenzeka ukuba incinci kakhuluImveliso yePCRinokwenziwa, okanye ayikho konke konke. Kubalulekile ke ngoko ukubaluleka kakhulu ukunciphisa ukuvela kwamanzi ngokuqinisekisa itywina elikhuselekileyo. Kule meko, isiciko esishushu se-Thermoycler kunye netywina lesikhephe liyasebenza ngesandla. Iinketho ezahlukeneyo zokutywina ziyafumanekaIpleyiti ye-PCR (Ikhonkco: inqaku lokutywina), apho itywina elilungileyo lifezekiswa ngokuhamba kwengqondo. Ezinye izivalo zinokulunga, okoko kunyanzeliswa koxinzelelo lomgaqo-nkqubo kunokulungiswa kwitywina elikhethiweyo.
Inkqubo yokubekwa emgangathweni isendaweni ukuze ikhusele iziphumo ezichanekileyo nezibuyisiweyo kwixesha elide. Oku kubandakanya ulondolozo rhoqo lwezixhobo ukuqinisekisa ukuba ihlala ikwimo elungileyo yokusebenza. Zonke izinto ezinokuthethwa zinokusetyenziswa ngokugqibeleleyo kuzo zonke eziveliswe ngamaqashiso, kwaye ukufumaneka kwabo ngokuthembekileyo kufuneka kuqinisekiswe.
IXESHA LOKUQALA: UNv-29-2022