Ungayisebenzisa njani ipleyiti ye-PCR?

I-PCR (i-polymerase chain) Iipleyiti zisetyenziselwa ukuqhuba iimvavanyo ze-PCR, ezisetyenziswa ngokubanzi kuphando lwe-molecular ukuze zikhuphe ukulandelelana kwe-DNA.

Nanga amanyathelo aqhelekileyo okusebenzisa aIplate ye-PCRUvavanyo oluqhelekileyo:

1. Lungiselela umxube wakho wokuphendula we-PCR: Lungisa umxube wakho wokuphendula we-PCR ngokwemigaqo yeProtokholi yakho, equka i-DNA ye-template yakho, i-prim primers, i-PREPS, i-Buffer kunye nezinye izongezelelo.
2. Yongeza umxube wokuphendula kwiplate ye-PCR: Sebenzisa ipipette yejelo elinamajelo okanye umbhobho wencwadi, yongeza umxube wequla leplate ye-PCR. Qiniseka ukuba ukuphepha ukuqaphela amaqamza omoya kumxube wokuphendula, njengoko oku kunokuchaphazela iziphumo zovavanyo lwakho.
3. Yongeza i-DNA yeThutyana yeTemplate yakho kwiMixhwam yokuphendula: Kuxhomekeka kuvavanyo lwakho, unokufuna ukongeza i-DNA yetemplate yakho kumxube wokuphendula. Ukuba usebenzisa i-pipette yejelo elinamajelo, qiniseka ukuba utshintshe iingcebiso phakathi kweesampulu ukunqanda ungcoliseko lwe-Cross.
4. Tywina ipleyiti: Nje ukuba wongeze umxube wokudibana kunye ne-template ye-DNA kwipleyiti ye-PCR, tywina ipleyiti enetywina elifanelekileyo, njenge-PCR Plethi yokutywina ifilimu okanye i-PCR Plate yokutsala ifilimu okanye i-PCR Playithi.
5. Beka ipleyiti kwi-ThermoycCler: Ekugqibeleni, beka ipleyiti ye-PCR kwi-thermoycler kwaye uqhube iqhinga lakho le-PCR, eliqhelekileyo eliquka uthotho lweDNA ukuba ikhule.

Emva kokuba i-PCR yokuphendula igqityiwe, unokuzihlalutya iimveliso usebenzisa iindlela ezahlukeneyo zobuchwephesha, njenge-gel electrophoresis okanye ukulandelelana. Qiniseka ukuba ulandela imigaqo-nkqubo ethile yovavanyo lwakho ukufumana iziphumo ezichanekileyo nezinokuthenjwa.

Suzhou ace biomedicalNgumenzi ophambili okhokelo lomgangatho ophezuluI-PCR IXABISO. Sinikezelwe ekuboneleleni ngezixhobo ezifanelekileyo kwaye ezifanelekileyo kwiimvavanyo zakho ze-PCR, kunye noluhlu lweemveliso ezenzelwe ukuhlangabezana neemfuno zabaphandi kwiindidi ezahlukeneyo.

I-PCR yethu ye-PCR iqukaIipleyiti ze-PCR, i-PCR Tubis, i-PCR Tubers, kunye neefilimu zokutywina. Zonke iimveliso zethu zenziwa ngezixhobo ezisemgangathweni eziphezulu, ziqinisekisa ukuba banokumelana ne-rigis yenkqubo ye-PCR kwaye bavelise iziphumo ezichanekileyo nezichanekileyo.

Kwi-suzhou ace biomedical, siyakuqonda ukubaluleka kokuchaneka kwe-PCR yakho. Kungenxa yoko le nto iipuzzle zethu zenziwa zinemigangatho ephezulu yomgangatho kwaye iphinde iqengqele ukuba ifezekise okanye idlule kulindelo lwakho. Iimveliso zethu zenzelwe ukuba zihambelane noluhlu olubanzi lwee-thermocys, zibenze bakuthengisele ukhetho lwabaphandi kwiilebhu ezahlukeneyo.

Enoba uqhuba uphando olusisiseko, indlela yokufumana isifo sekliniki, okanye ezinye iinkqubo, i-suzhou ace biomedicacal inezinto ezinokufunyanwa kuzo. Sizimisele ukubonelela ngeemveliso ezizodwa kunye nenkonzo yabathengi, kwaye sinebhongo ngokuba liqabane elithembekileyo kubaphandi kwihlabathi liphela.

Nxibelelana nathi namhlanje ukuze ufunde ngakumbi malunga ne-PCR yethu ye-PCR kunye nendlela esinokuxhasa ngayo uphando lwakho.