Uyisebenzisa njani ipleyiti yePCR ukwenza umfuniselo?

I-PCR (i-polymerase chain reaction) iipleyiti zisetyenziselwa ukuqhuba imifuniselo ye-PCR, esetyenziswa ngokubanzi kuphando lwebhayoloji ye-molekyuli ukukhulisa ukulandelelana kwe-DNA.

Nanga amanyathelo aqhelekileyo okusebenzisa aPCR ipleyitikuvavanyo oluqhelekileyo:

1. Lungiselela umxube wokusabela kwe-PCR yakho: Lungiselela umxube wokusabela kwe-PCR ngokweprothokholi yovavanyo lwakho, oluquka itemplate yeDNA, iiprimer zePCR, dNTPs, Taq polymerase, buffer kunye nezinye izongezo.
2. Yongeza umxube wokuphendula kwi-PCR plate: Ukusebenzisa i-pipette ye-multi-channel okanye i-pipette ye-manual, yongeza umxube wokuphendula kwimithombo ye-PCR plate. Qinisekisa ukunqanda ukwazisa amaqamza omoya kumxube wokusabela, njengoko oku kunokuchaphazela iziphumo zovavanyo lwakho.
3. Yongeza itemplate yakho yeDNA kumxube wokusabela: Kuxhomekeke kuvavanyo lwakho, unokufuna ukongeza itemplate yakho yeDNA kumxube wokusabela. Ukuba usebenzisa i-pipette yamajelo amaninzi, qiniseka ukuba utshintshe iingcebiso phakathi kweesampuli ukuphepha ukungcoliseka.
4. Vala ipleyiti: Emva kokuba wongeze umxube wokusabela kunye netemplate yeDNA kwipleyiti yePCR, tywina ipleyiti ngetywina elifanelekileyo, elifana nefilimu yokutywina yePCR okanye i-cap strip.
5. Beka ipleyiti kwi-thermocycler: Okokugqibela, beka ipleyiti ye-PCR etywiniweyo kwi-thermocycler kwaye uqhube inkqubo yakho ye-PCR, ebandakanya uchungechunge lwemijikelezo yobushushu evumela ukuba i-DNA yandiswe.

Emva kokuba impendulo ye-PCR igqityiwe, unokuhlalutya iimveliso usebenzisa iindlela ezahlukeneyo, ezifana ne-gel electrophoresis okanye ulandelelwano. Qiniseka ukuba ulandela imigaqo ethile yovavanyo lwakho ukuze ufumane iziphumo ezichanekileyo nezithembekileyo.

Suzhou Ace Biomedicalngumvelisi ophambili womgangatho ophezuluPCR izinto ezisetyenziswayo. Sizinikezele ekuboneleleni ngezixhobo ezithembekileyo nezisebenzayo kwimifuniselo yakho yePCR, ngoluhlu lweemveliso eziyilelwe ukuhlangabezana neemfuno zabaphandi kwiinkalo ezahlukeneyo.

PCR zethu ezisetyenziswayo ziqukaIipleyiti zePCR, iityhubhu zePCR, imichilo yetyhubhu yePCR, kunye neefilimu zokutywina. Zonke iimveliso zethu zenziwe ngezinto ezisemgangathweni ophezulu, ziqinisekisa ukuba ziyakwazi ukumelana nobunzima benkqubo yePCR kwaye zivelise iziphumo ezihambelanayo nezichanekileyo.

ESuzhou Ace Biomedical, siyakuqonda ukubaluleka kokuchaneka kwimifuniselo yakho yePCR. Yiyo loo nto izinto zethu ezityiwayo zePCR zenziwe ngeyona migangatho iphezulu yomgangatho kwaye zivavanywa ngokungqongqo ukuqinisekisa ukuba ziyahlangabezana okanye zigqithise ubulindele. Iimveliso zethu zikwayilelwe ukuba zihambelane noluhlu olubanzi lwee-thermocyclers, zizenza ukhetho oluguquguqukayo lwabaphandi kwiilebhu ezahlukeneyo.

Ingaba uqhuba uphando olusisiseko, ukuxilongwa kweklinikhi, okanye ezinye izicelo, i-Suzhou Ace Biomedical ine-PCR consumables oyifunayo ukufezekisa iinjongo zakho. Sizimisele ukubonelela ngeemveliso ezikhethekileyo kunye nenkonzo yabathengi, kwaye sinebhongo ngokuba liqabane elithembekileyo kubaphandi kwihlabathi liphela.

Qhagamshelana nathi namhlanje ukuze ufunde ngakumbi malunga nezinto ezityiwayo zePCR kunye nendlela esinokuxhasa ngayo uphando lwakho.