UNGAYEKA NJANI UKUHLANGABEZA I-PLATE ENGUNZULU 96

Zingaphi iiyure ngeveki ophulukana nazo kwiipleyiti zequla elinzulu?

Umzabalazo yinyani. Nokuba zingaphi iipipettes okanye iipleyiti ozilayishile kuphando lwakho okanye emsebenzini, ingqondo yakho inokuqalisa ukudlala amaqhinga kuwe xa kufikwa ekulayisheni ipleyiti eyoyikekayo ye-96 enzulu.

Kulula kakhulu ukongeza iivolumu kwiqula elingalunganga okanye kumqolo ongalunganga. Kulula nje ukuphinda ngempazamo ipleyiti yomthombo ofanayo onzulu.

Okanye ulayisha yonke isampuli engalunganga kumaqula amaninzi, kuxabisa iiyure zakho zokusebenza.

Okanye, mhlawumbi wenze yonke into ngokufanelekileyo, kodwa uqala ngokuzicingela okwesibini. Ukuqala phantsi.

Ixesha lakho lixabiseke kakhulu. Iirejenti zakho zixabiseke kakhulu. Kwaye, okona kubaluleke kakhulu, idatha yakho ixabiseke kakhulu.

Akunyanzelekanga ukuba sikuxelele ukuba yinkcitho yexesha eli, xa uqhele ukwenza iireagents kwaye uxube. Kwaye, ayiziva imnandi kakhulu kwinqanaba lokuzithemba.

Nanga amacebiso angcono kunye namaqhinga avela kwabanye onokuqala ukuwabandakanya kwindlela yakho yaselebhu.

Yintoni ipleyiti yequla enzulu engama-96?

Isiseko esihlala singahoywa kwiilebhu nakwiindawo zophando kuyo yonke indawo, iipleyiti zequla ezinzulu zilungele ixesha elifutshane kunye nexesha elide lokugcina isampulu, ukulungiswa, kunye nokuxuba. Banokuba nesikwere somthombo okanye umzantsi ongqukuva.

Ukusetyenziswa kwazo kuyahluka, kodwa zihlala zisetyenziswa kwizicelo zesayensi yobomi kunye nokusetyenziswa kophando, kubandakanya:

• Umsebenzi wenkcubeko yeeseli zezicubu kunye nohlalutyo lweeseli
• Iimvavanyo ze-enzyme
• Izifundo zeProteomics
• Amadama e-reagent
• Ugcino olukhuselekileyo lwesampulu (kubandakanya ugcino cryogenic)

Iingcebiso eziphezulu kunye namaqhinga okoyisa iimpazamo zepleyiti ezinzulu ezingama-96

Siqulunqe uluhlu lweendlela eziphambili kunye neendlela ezivela koogxa bakho:

1. Jonga ingqondo yakho kwaye uhlale ugxile:Njengayo nayiphi na into ebomini, iimpazamo zikholisa ukwenzeka xa udiniwe, uxinezelekile, okanye uphazamisekile (… okanye konke oku kungasentla). Yeka ukuzikhathaza ngokukhawulezisa umsebenzi wakho. Yehlisa isantya, kwaye ucinge ngenyathelo ngalinye ngocoselelo ngakumbi. Kwaye uhlale ugxininise. Ukuthetha nokusebenza kwenza ukuba eminye imisebenzi ihambe ngokukhawuleza, kodwa hayi ngalo msebenzi. Abanye abaphandi baxhoma "Akuthethi" babhalise njengoko bephakathi kulo msebenzi. Umculo opholileyo (ingakumbi izixhobo zomculo) uyakhuthazwa, nangona kunjalo, ukuba ufuna ingxolo engemva ngelixa usebenza!
2. Tshatisa iingcebiso zakho zepipette kunye namaqula ahambelanayo:Ibhokisi entsha yepipette yeyona ilungileyo kwiipleyiti zequla elinzulu. Tshatisa iqula nebhokisi njengoko usiya. Yiba nebhokisi yogcino kwindawo yokulinda ukuze uphelelwe, ke akufuneki uchithe inkqubo yakho ukuba ufuna ngaphezulu. Sebenzisa iingcebiso zepipette ukugcina umkhondo wokubala kakuhle.
3. Yibhale:Yenza i-Excel sheet yomxube oyinkosi, kunye neemephu zepleyiti ezinzulu ezingama-96. Iqula ngalinye linegama lee-primers kunye neesampuli. Cwangcisa yonke imixube yakho ngendlela enengqiqo, kunye nekhowudi yombala kwiseti nganye yeprimer (ukuba usebenzisa ngaphezu kwesinye). Yiza neli phepha kunye nawe elebhu, kwaye ujonge uphawu xa usiya. Ungaphinda ubhale iimali ze-reagent kwi-post-kwaye uyigcine ecaleni kwakho njengesitshixo sakho sesampulu njengoko ulayisha. Khetha isixokelelwano sokusebenza ngazo (umz. ngokwealfabhethi okanye ngokwamanani, kuxhomekeke kwindlela ezikhowudwa ngayo) kwaye ungaze uphambuke kwisixokelelwano sakho. Xa usenza umxube, beka yonke into ngokulandelelana kwi-rack yakho, emva koko uyisuse kwikona ekude xa uyenziwe.
4. Iteyiphu ngumhlobo wakho omtsha wenene:Cima ipleyiti xa iyonke, ecaleni kwendawo oyilayishayo. Sebenza ngapha kweplate ngale ndlela, uhambisa iteyiphu rhoqo xa icandelo ligqityiwe. Ungaleyibhile iteyiphu yakho (umz. A – H, 1 – 12) ukukunceda uhlale endleleni.
   Umzekelo, ngelixa ulayisha i-Gene A mastermix kwikholamu 1 kunye ne-2 yepleyiti yakho enzulu, thatha iteyiphu kuqala kwaye ugqume ngobunono ikholamu yesi-3 neyesi-4. Unokwenza le kholamu enye ngexesha, ukuze uhlale ulungelelene. Inceda ukuhlala ulungelelene ngexesha lamaqula aphakathi anzima. Khawukhumbule nje ukubamba ipleyiti phantsi ngokuthe gqolo xa ususa iteyiphu yakho, ukunqanda ukutshiza.
5. Qhubeka nayo:Ukuba uyaqonda ukuba inkqubo yakho ayisebenzi, musa ukuyitshintsha phakathi. Yitshintshe ngaphambi okanye emva, kodwa ungaze uye embindini (kukhokelela ekubhidekeni okukhulu!).
6. Ziqhelanise:Hlala uhambelana nenkqubo oyikhethayo. Ukuzibophelela la manyathelo kwimemori yemisipha kuya kuthatha ixesha, kodwa ekuhambeni kwexesha kufuneka uqale ukubona ukuphucuka okubalulekileyo emsebenzini wakho (kwaye kuncinci kakhulu ukukhungatheka kwindawo yakho yokusebenza!)

Khetha isixhobo esifanelekileyo:

Ukusuka kwizinto zokusebenza ukuya kumgangatho, amaqula ajikelezayo okanye i-conical bottom, kukho iindlela ezahlukeneyo zokukhetha xa u-odola ipleyiti enzulu ye-96.

Ezinye izinto eziqwalaselwayo ziquka:

• Material: Yeyiphi iisampulu oyisebenzisayo? Ngaba iqula lakho elinzulu lifuna ukugqunywa nge-lobind okanye yenziwe ngesilicone?
• Ubungakanani: Ungakanani umthamo ofunekayo ukuze ungene kwipleyiti yakho enzulu ye-96 PCR?
• Ubushushu: Ngawaphi amaqondo obushushu amaqula akho anzulu afuna ukumelana nawo?
• Ngawaphi amandla e-centrifugation enokumelana nepleyiti yakho yequla enzulu engama-96?

Nantsi into eyenziwa zizazinzulu ezininzi kwizicelo jikelele:

Ezi alula 96 Deep Well Plates

Ezi pleyiti zinzulu zinceda njani iilebhu kunye nabaphathi balebhu:

• Anindlela elulaukuqokelela kunye nokulungisa iisampulu (kuba akukho kunqongophala kwezo zinto zenzeka kwilabhu yakho yonke imihla)
• Fumana i-labspace exabisekileyo emva, kunye nomthamo oqinileyo wokupakisha okwenza kube lula ukuyigcina kunangaphambili
• Kuphephe ukuchitheka ngeukuxuba okuphuculweyoyeesampuli zakho zolwelo ezincinci
• A uyilo ukubakunciphisa ukugcinwa kweendonga, ngoko uchitha ngaphantsi kwesampulu yakho
• Hlawula33% ngaphantsikunokuba ungenza kwezinye iibrendi eziphambili

Iimpawu ziquka:

• Umzantsi ongqukuva
• Inokufakwa emkhenkceni okanye ifakwe efrijini (ukuya kuthi ga kwi -80 C)
• Ukuzinza - abayi kusabela kunye ne-solvents kwi-plate
• Bandakanya iintsimbi ezinzima zokuphucula ngokukhuselekileyo
• Eyilwe ngokobungakanani bomgangatho wamazwe ngamazwe (i-SBS), ibenza bafanelekele iindawo zokusebenza ezizenzekelayo
• Vumela ukugcinwa kolwelo okuncinci kwesampulu yakho ezindongeni

Ukukhetha ipleyiti elungileyo kunokukunceda uphephe:

• Uphose amanqaku edatha
• Ukwenziwa kwakhona kwesampuli
• Ukuhamba okucothayo
• Uphose imihla ebekiweyo yeprojekthi

Ulonwabele uphando

Amacwecwe angama-96 anzulu afumaneka kwiilebhu nakumaziko ophando kwihlabathi jikelele. Bangagcina ixesha, umzamo, kunye nendawo yokugcina, kodwa inkqubo efanelekileyo ibalulekile njengoko ugqibezela umsebenzi wakho.

Ukusuka kumthamo wokugcina owandisiweyo, ukuya kumxube ophuculweyo, iipleyiti zequla ezinzulu zifanelekile kwikhemistri edibeneyo kunye nezicelo zethala leencwadi, ezixhathisayo kwiikhemikhali ezininzi, i-solvents, kunye ne-alcohols esetyenziswa kwi-combinator chemistry.

Ilungele ukuqokelelwa kwesampulu, ukulungiswa kwesampulu, kunye nokugcinwa kwesampulu yexesha elide (okanye ixesha elifutshane), iipleyiti zequla elinzulu kunye neemethi zokutywina zinokuphucula ukuhamba komsebenzi, kwaye ipleyiti elungileyo enzulu iya kukunceda uvelise eyona datha iphezulu yomgangatho wezicelo eziqhelekileyo isayensi yobomi (nangaphaya).