Uyitywina njani iPleti yePCR

Intshayelelo

Iipleyiti zePCR, isiseko selabhoratri iminyaka emininzi, siya sixhaphake ngakumbi kwimeko yangoku njengoko iilabhoratri zinyusa umthamo wazo kwaye zisebenzisa i-automation ngaphakathi kokuhamba kwazo. Ukufezekisa ezi njongo ngexa kugcinwe ukuchaneka kunye nemfezeko yemifuniselo kunokuba nzima. Enye yeendawo eziqhelekileyo apho iimpazamo zinokungena khona kukutywinwa kweIipleyiti zePCR, ngobuchule obuhlwempuzekileyo obuvumela ukuba iisampulu zibe ngumphunga, ukuguqula i-pH kwaye ke ngoko kuphazamise imisebenzi ye-enzymatic, kunye nokumema ukungcoliseka. Ukufunda indlela yokutywina aPCR ipleyitingokuchanekileyo kuphelisa le mingcipheko kwaye iqinisekisa iziphumo ezinokuphinda zikwazi ukuveliswa kwakhona.

Fumana itywina eliLungileyo kwiPleti yePCR yakho

I-Plate Caps vs. I-Film Seals vs. I-Lids
Iicapsziyindlela elungileyo yokutywina ipleyiti yakho ngetywina eliqinileyo, ngeli lixa ikunika uguquko lokuyivula ngokulula kwaye uyivale kwakhona ipleyiti njengoko ufuna ngaphandle kwenkunkuma. Nangona kunjalo, ii-caps zineengxaki ezimbalwa eziphambili.

Okokuqala, kuya kufuneka uthenge i-cap ethile ehambelanayo, eyenza ukuba ingaguquguquki. Kuya kufuneka uqiniseke ukuba ikepusi oyikhethayo ilingana nepleyiti, exhomekeke kumvelisi wayo, kwaye ukhethe nokuba idomile okanye ithe tyaba ngokusekelwe kwisixhobo sobushushu obusebenzisayo.

Okwesibini, ukusebenzisa ii-caps kwi-plate kunokuphindaphinda kakhulu kwaye kudinise, kunye nomngcipheko wokungcoliswa komnqamlezo ukuba ubeka i-cap engafanelekanga kwi-well engalunganga.

Ngelixa amatywina efilimu engabhetyebhetye kancinci malunga nokususa kunye nokutshintshwa, aguquguquka kakhulu njengoko aya kungena kulo naluphi na uhlobo lwepleyiti yePCR, kungajongwanga ukuba ngubani umenzi. Zinokunqunyulwa ngokulula, zizenze zisebenze kakhulu.

Enye inketho sisiciko sepleyiti. Ezi zibonelela ngokhuseleko oluncinane oluzigqumayo kunye netywina, kwaye zisetyenziselwa kuphela ukhuseleko lwexeshana elifutshane ukuthintela ukosuleleka.

I-Optical vs. I-Foil Film Seals

Nokuba ufuna i-optical, itywina elicacileyo okanye iifilimu ye-aluminium foilukutywina ipleyiti yakho kugqitywe ngefomathi yakho yovavanyo.Iifilimu zokutywina zamehloziselubala ukuvumela ukuba uqwalasele iisampuli, ngexa usazikhusela kwaye unqanda ukuvela komphunga. Zikwaluncedo ngakumbi kuvavanyo lwe-qPCR olubandakanya ukwenza imilinganiselo echanekileyo kakhulu ye-fluorescence ngokuthe ngqo kwipleyiti, apho uya kudinga ifilimu yokutywina ehluza i-fluorescence encinci kangangoko kunokwenzeka. Kubalulekile ukuqinisekisa ukuba itywina okanye ikepusi oyisebenzisayo inomgangatho ophezulu ngokwaneleyo wokucaca okubonakalayo ukuqinisekisa ukuba ufundo luchanekile.

Iifilim zefoyile zifanelekile kuzo naziphi na iisampulu ezibuthathaka okanye ezimele zigcinwe ngaphantsi kwe-80°C. Ngesi sizathu, uninzi lweesampulu ezimiselwe ukugcinwa kwexesha elide ziya kufuna ifilimu yefoyile. Iifilim zefoyile nazo ziyakwazi ukugqobhoza, nto leyo eluncedo ekuhloleni amaqula ngamanye, okanye ukuhanjiswa kweesampulu ngeenaliti. Oku kunokwenzeka ngesandla okanye njengenxalenye yeqonga lerobhothi.

Kwakhona qwalasela ukuba izinto ezinobundlongondlongo ezibandakanya iiasidi, iziseko okanye izinyibilikisi ziya kufuna itywina elinokumelana nazo, kwimeko apho isitywina sefoyile sifanelekile ngakumbi.

I-Adhesive vs. Ifilimu yokutywinwa kobushushu
Iimpawu zefilimu ezincamathelayozithe ngqo kakhulu kwaye kulula ukuzisebenzisa. Into oyifunayo kukuba umsebenzisi afake isitywina kwipleyiti, kwaye usebenzise isixhobo esilula sokufaka isicelo ukucinezela kwaye wenze itywina eliqinileyo.

Izitywina zobushushu zihambele phambili kakhulu, zibonelela ngesitywina esihlala ixesha elide esithe sacutha amaqondo okuphuma komphunga xa kuthelekiswa nesitywina esincamathelayo esiqhelekileyo. Olu khetho lufanelekile ukuba ujonge ukugcina iisampulu kwixesha elide, nangona oku kuza kunye nemfuneko eyongezelelweyo yezixhobo zokutywina iipleyiti.

Uyitywina njani iPleti yePCR

Indlela yokutywinwa kwePlate

Ukuzincamathelisa

1. Qinisekisa ukuba usebenza kumphezulu osicaba nozinzileyo

2. Susa ifilimu kwi-package yayo, kwaye ususe ukuxhaswa

3. Beka itywina ngononophelo epleyitini, ngelixa uqinisekisa ukuba onke amaqula agqunyiwe

4. Sebenzisa isixhobo sokufaka isicelo ukufaka uxinzelelo kwipleyiti. Qala ukusuka kwelinye icala kwaye usebenze indlela yakho ukuya kwelinye, ucinezele ngokulinganayo

5. Phinda oku izihlandlo ezininzi

6. Jikelezisa umfaki-sicelo kumaqula angaphandle, ukuqinisekisa ukuba nawo atywinwe ngokufanelekileyo.

Amatywina obushushu

Izitywina zokushisa zisebenza ngokunyibilika ifilimu ukuya kwi-rim yequla ngalinye, ngoncedo lwe-plate sealer. Ukusebenzisa i-heat sealer, bhekisa kwimiyalelo enikwe ngumenzi wesixhobo. Qinisekisa ukuba umenzi ofumana kuye izixhobo zakho uhloniphekile, njengoko kubaluleke kakhulu ukuba itywina ilungile, iyasebenza kwaye ayinamanzi.

Ipleyiti Ukutywinwa Iingcebiso eziphezulu

a. Xa ufaka uxinzelelo kwitywina, yiya kuzo zombini iindlela ezithe tye nezithe nkqo ukuze uqinisekise itywina elifanelekileyo.

b. Ihlala iluqheliselo olulungileyo ukuqhuba uvavanyo lwento oyenzayo, kwaye oku akufani nokutywinwa kwepleyiti. Vavanya ngepleyiti engenanto phambi kokuba usebenzise enye enesampulu.

c. Xa uvavanya, susa isitywina kwaye ujonge ukubona ukuba i-adhesive ibambelele ngokufanelekileyo, kungekho zithuba. Kukho ukubonakaliswa okubonakalayo koku kuxwebhu lokuqala lwereferensi. Ukuba awuzange utywine iplate ngokufanelekileyo, xa ususa isitywina kuya kubakho izikhewu apho i-adhesive ingazange idibanise ngokupheleleyo kwi-plate.

d. Ukuthunyelwa kunye nokuthutha iisampulu, unokufumanisa ukuba luncedo ukufaka isitywina seplastiki phezu kwetywina le-foil ukukhusela okongeziweyo (ingakumbi ekubhobhozeni).

e. Ngalo lonke ixesha qinisekisa ukuba akukho ziqhuma okanye imibimbi xa ufaka ifilim - ezi zinto ziya kubangela ukuvuza kunye nokunyuka komphunga.