Uwenziwa njani uPipette amaVolumes amaNcinci ngePipettes yeManuwali ephathwa ngesandla

Njengoko kugxininiswa ngakumbi ekunciphiseni ii-reagents kunye neendleko, amanani amancinci afunwa kakhulu, umzekelo, ukulungiselela i-PCR Mastermix okanye i-enzyme reactions. Kodwa ukubhobhoza imibhobho emincinci ukusuka kwi-0.2 - 5 µL ibeka imingeni emitsha yokuchaneka kombhobho kunye nokuchaneka. Ezi ngongoma zilandelayo zibalulekile:

1. I-Pipette kunye nobungakanani bencam: Soloko ukhetha i-pipette enomthamo ophantsi onokwenzeka kunye nencam encinci yokugcina umqamelo womoya umncinci kangangoko. Xa ufaka i-1 µL umz., khetha i-0.25 – 2.5 µL ipipetti kunye nencam ehambelanayo kune-1 – 10 µL ipiipi.
2. Ukulinganisa kunye nokugcinwa: Kubalulekile ukuba iipayipi zakho zilinganiswe ngokufanelekileyo kwaye zigcinwe. Uhlengahlengiso oluncinci kunye neengxenye eziqhekekileyo kwi-pipette zikhokelela ekunyukeni okukhulu kwemilinganiselo yempazamo ecwangcisiweyo kunye ne-random. Ulungelelwaniso ngokwe-ISO 8655 kufuneka lwenziwe kube kanye ngonyaka.
3. Iipipette zokufuduka ezilungileyo: Khangela ukuba unepipette efanelekileyo yokufuduka enoluhlu oluphantsi lwevolumu kwilebhu yakho. Ngokuqhelekileyo, ukusebenzisa olu hlobo lwe-pipette lukhokelela kwisiphumo esingcono sepayipi ngokubhekiselele ngokuchanekileyo kunye nokuchaneka kune-classic air-cushion pipettes.
4. Zama ukusebenzisa imiqulu emikhulu: Unokucinga ngokuhlambulula isampuli yakho kwi-pipette imiqulu emikhulu kunye nobuninzi obufanayo kwimpendulo yokugqibela. Oku kunokunciphisa iimpazamo zemibhobho ngeesampulu ezincinci kakhulu.

Ukongeza kwisixhobo esilungileyo, umphandi kufuneka abe nobuchwephesha obuhle kakhulu bepayipi. Nika ingqwalasela eyodwa kula manyathelo alandelayo:

1. Ingcebiso yokuncamathisela: Musa ukujamza i-pipette kwincam njengoko oku kungonakalisa isiphelo sencam ecolekileyo kubangele ukuba umqadi wolwelo uqondiswe ngokutsha okanye wonakalise i-orifice. Faka kuphela uxinzelelo olulula xa udibanisa isicatshulwa kwaye usebenzise i-pipette ene-spring-loaded tip cone.
2. Ukubamba i-pipette: Musa ukubamba i-pipette esandleni sakho ngelixa ulinde i-centrifuge, i-cycler, njl.
3. Ukumanzisa kwangaphambili: Ukufuma komoya ngaphakathi kwincam kunye ne-pipette ilungiselela i-tip yesampuli kwaye igweme ukukhupha umoya xa unqwenela umthamo wokudlulisa.
4. Ukunqwenela ngokuthe nkqo: Oku kubaluleke kakhulu xa uphatha amanani amancinci ukuphepha umphumo we-capillary owenzeka xa i-pipette ibanjwe kwi-angle.
5. Ubunzulu bokuntywiliselwa: ntywilisela incam kancinane kangangoko ukuthintela ulwelo ukuba lungene kwincam ngenxa yesiphumo secapillary. Umgaqo wesithupha: Incinci incam kunye nomthamo, isezantsi ubunzulu bokuntywiliselwa. Sincoma ubuninzi be-2 mm xa ufaka imibhobho emincinci emincinci.
6. Ukukhupha kwi-angle ye-45 °: Ukuphuma okulungileyo kokuphuma kwamanzi kuqinisekisiwe xa i-pipette ibanjwe kwi-angle ye-45 °.
7. Uqhagamshelwano nodonga lwenqanawa okanye umphezulu wolwelo: Imithamo emincinci ingakhutshwa ngokufanelekileyo kuphela xa incam ibanjwe eludongeni lwenqanawa, okanye intywiliselwe kulwelo. Nokuba ithontsi lokugqibela elivela kwincam lingasasazwa ngokuchanekileyo.
8. Ukuvuthela: Ukuvuthela kunyanzelekile emva kokukhupha imithamo ephantsi ukukhupha nethontsi lokugqibela lolwelo elikhoyo kwincam. Ukuvuthela kufuneka kuqhutywe ngokuchasene nodonga lwenqanawa. Kulumkele ukuzisa amaqamza omoya kwisampulu xa uvuthela kumphezulu wolwelo.