Ukuchaneka kunye nokuchaneka kwe-pipette engcono kakhulu yokulinganisa inokucinywa ukuba ukhetha uhlobo olungalunganga lweengcebiso. Ngokuxhomekeke kuvavanyo olwenzayo, uhlobo olungalunganga lweengcebiso lunokwenza ukuba i-pipette yakho ibe ngumthombo wongcoliseko, ikhokelela kwinkcitho yeesampulu ezixabisekileyo okanye i-reagents-okanye ibangele ingozi emzimbeni ngendlela yokulimala okuphindaphindiweyo (RSI). Kukho iintlobo ezininzi zeengcebiso onokukhetha kuzo. Uyazi njani ukuba yeyiphi eyona nto ingcono kwi-pipette yakho kunye nemeko? Ungaze woyike, yile nto silapha ngayo.
- 1) Khetha iingcebiso ezikumgangatho ophezulu wepipette ngokuchanekileyo kunye nokuchaneka
- 2) Universal okanye Pipette iingcebiso ezithile?
- 3) Isihluzi kunye neengcebiso zepipette ezingahluziyo. Izinto eziluncedo kunye neziphazamisi
- 4) Iingcebiso zokugcina eziphantsi
- 5) Iingcebiso ze-ergonomic
1) Khetha iingcebiso eziphezulu zepipette ngokuchanekileyo kunye nokuchaneka
Uqwalaselo lokuqala oluthi luthi qatha engqondweni xa ucinga malunga nokuba loluphi uhlobo lwengcebiso onokuthi ukhethe ukuchaneka nokuchaneka. Ukuba kukho nayiphi na i-batch-to-batch, okanye ngaphakathi kwe-batch, ukuhluka kwimilo yeengcebiso ze-pipette, ngokoumbhobho wakho awuyi kuchaneka. Ukuchaneka kwepipette yakho kunokuchaphazelekaukuba incam ayihambisani nepipette yakho ngokufanelekileyo. Ukuba kukho itywina elibi phakathi komgqomo wakho wepipette kunye nencam, umoya otsaliweyo unokubaleka kwaye umthamo ochanekileyo wolwelo awunqweneleki. Ke ngoko, umthamo wokugqibela okhutshiweyo awuchanekanga ngokupheleleyo. Ukukhetha ingcebiso efanelekileyo kwi-pipette yakho ingaba lishishini elikhohlisayo.
Oko kusizisa kumbuzo….
2) Iingcebiso ze-Universal okanye zePipette?
Olona khetho lungcono kwipipette yakho kunye nesicelo kukusebenzisa iingcebiso ezikumgangatho ophezulu. Ezi ngcebiso zendalo yonke zinokusetyenziswa kunye nee-micropipettes ezininzi kwiimarike. Iingcebiso zendalo yonke zenzelwe ukulingana ngokukhuselekileyo kunye nokuqiniswa kuzo zonke iibhobho zepipette, ezihluka kancinci ububanzi ukusuka kumenzi ukuya kumenzi. Ngokomzekelo, iingcebiso ngeteknoloji ye-FlexFit ziguquguqukayo ekupheleni kwe-tip (oko kukuthi, kufuphi ne-barrel), ebanika ukulungeleka okungcono kunye noluhlu olubanzi lweentlobo ze-pipette. Kwiikliniki zeLab, unokufumana iingcebiso zendalo yonke kunye nazo zonke iimpawu ezixoxwe ngezantsi (umqobo we-aerosol, uphumelele, i-ergonomic, njl.).
3) Iingcebiso zokucoca kunye nezingahluziyo. Izinto eziluncedo kunye nokuphazamiseka
Iingcebiso zokuthintela, okanye iingcebiso zokucoca, zenzelwe iimeko ezahlukeneyo. Ukuba uya kuba umbhobho into onokuyenzaungcolise ipipette yakho-umzekelo, iikhemikhali eziguquguqukayo, ezinobungozi, okanye ezibonisayo-ke uya kufuna ukuqwalasela iingcebiso zokuthintela ukukhusela i-pipette yakho kunye neesampuli zakho.
Iingcebiso zesihluzi zithintela ungcoliseko lwePCR
Iingcebiso ze-Aerosol Barrier, ebizwa ngokubaiingcebiso zokucoca ipayipi, zifakelwe isihluzi ngaphakathi kwendawo ekufutshane yencam. Isihluzi sikhusela iipipettes zakho kwi-aerosols kunye ne-aspirating volative or viscous solutions kwi-barrel, zonke ezinokuthi zingcolise kwaye zonakalisa i-pipette. Ezi ngcebiso zihlala zisiza ngaphambi kokwenziwa inzala kunye ne-DNase/RNase-free. Nangona kunjalo, "umqobo" yinto encinci yegama elingalunganga kwezinye zezi ngcebiso. Iingcebiso ezithile kuphela eziphezulu zibonelela ngomqobo wokutywina oyinyaniso. Uninzi lwamacebo acotha kuphela ulwelo ekungeneni kumbhobho wepipette. Isithintelo sokucoca kwezi ngcebiso sibenza ukuba bakhethe kwizicelo ezinovakalelo, njenge qPCR. Umqobo uthintela ukungcoliswa kwe-PCR ngokumisa isampuli ye-carryover kwi-pipette, eya kukunika iziphumo ezinamandla ngakumbi. Kwakhona, khumbula ukuqhuba ulawulo olulungileyo lwe-PCR kunye nolawulo olubi ukufumana isampulu ye-carryover. Ukongeza, iingcebiso zokucoca zilungile 'amavili oqeqesho' kwabaqalayo. Amaxesha amaninzi ukungcoliseka kwe-pipette kwenzeka xa ilungu elitsha elebhu linqwenela ngengozi ulwelo kwi-pipette ngokwayo. Kulula kakhulu, kwaye kuyabiza, ukulahla i-tip kunokuthumela yonke i-pipette ukuze ilungiswe kuba ulwelo lukwipiston.
4) Iingcebiso zokugcina eziphantsi
Nokuba yeyiphi na ingcebiso oyikhethayo, ukugcinwa okuphantsi luphawu oluphambili. Iingcebiso zokugcinwa okuphantsi zenza kanye njengoko igama libonisa-gcina amanqanaba aphantsi olwelo. Ukuba ukhe wajonga incam yombhobho osemgangathweni, unokubona intwana yolwelo eshiyekileyo emva kokunikezela. Iingcebiso zokugcinwa okuphantsi zinciphisa oku kungenzeki ngenxa yokuba zinezongezo zeplastiki ze-hydrophobic ezigcina i-liquid ekunamatheleni ngaphakathi kweengcebiso.
5) Iingcebiso ze-Ergonomic
Ukwenza imisebenzi ephindaphindiweyo, njengepayipi, kunokubangela umonakalo kumalungu kwaye kubangele ukulimala okuphindaphindiweyo koxinzelelo (RSI). Ngenxa yoko, iinkampani zenze iingcebiso ze-ergonomic ezifuna ukufakwa okuphantsi kunye ne-ejection forces kwaye, ngoko ke, ukunciphisa umngcipheko we-RSI. Oko kwathiwa, eli nqaku lonke libuyela ekulinganeni kakuhle. Ingcebiso eyenzelwe ngokukodwa ukulungelelanisa i-pipette yakho ngokufanelekileyo yinkcazo ye-ergonomic tip.
Ixesha lokuposa: May-10-2022