Yintoni iCryovials?
Iibhotile zeCryogenic zokugcinazincinci, izikhongozeli ezinezingxobo kunye nezisilinda eziyilelwe ukugcina nokugcina iisampuli kumaqondo obushushu asezantsi kakhulu. Nangona ngokwesiko ezi bhotile zenziwe ngeglasi, ngoku ziqhele ukwenziwa ngepolypropylene ukuze kube lula kunye neendleko. IiCryovials ziyilwe ngononophelo ukuba zimelane namaqondo obushushu asezantsi njenge -196℃, kwaye zikwazi ukwamkela iintlobo ngeentlobo zeeseli. Ezi ziyohluka ukusuka kwiiseli ze-stem zokuxilongwa, ii-microorganisms, iiseli eziphambili ukuya kwiiseli zeseli. Ngaphaya koko, kusenokubakho ii-organisms ezincinci ze-multicellular ezigcinwe ngaphakathiiibhotile zokugcina i-cryogenic, kunye ne-nucleic acid kunye neeprotheni ezifuna ukugcinwa kumazinga okushisa okugcina i-cryogenic.
Iibhotile zokugcina i-Cryogenic ziza ngeendlela ezahlukeneyo, kwaye ukufumana uhlobo oluchanekileyo oluzalisekisa zonke iimfuno zakho kuya kuqinisekisa ukuba ugcina ingqibelelo yesampula ngaphandle kokuhlawula ngaphezulu. Funda ngenqaku lethu ukuze ufunde ngakumbi malunga nezona zinto ziphambili ekuthatheni ingqalelo kuzo xa ukhetha i-cryovial efanelekileyo kwisicelo sakho selabhoratri.
Iipropati zeCryogenic Vial ekufuneka ziqwalaselwe
Yangaphandle vs Imisonto yaNgaphakathi
Abantu bahlala besenza olu khetho lusekwe kukhetho lomntu, kodwa eneneni kukho umahluko ongundoqo wokusebenza ekufuneka uqwalaselwe phakathi kwezi ntlobo zimbini zemisonto.
Iilabhoratri ezininzi zihlala zikhetha iibhotile ezithungelwe ngaphakathi ukunciphisa indawo yokugcina ityhubhu ukuvumela ukungena ngcono kwiibhokisi zesikhenkcisi. Ngaphandle koku, unokucinga ukuba ukhetho olufakwe ngaphandle lolona khetho lungcono kuwe. Bacingelwa ukuba baphathe umngcipheko ophantsi wongcoliseko, ngenxa yoyilo olwenza ukuba kube nzima ngakumbi kuyo nayiphi na into ngaphandle kwesampulu yokungena kwi-vial.
Iibhotile ezinemisonto yangaphandle zikhethwa ngokubanzi kwizicelo ze-genomic, kodwa nayiphi na inketho ithathwa njengefanelekileyo kwi-biobanking kunye nezinye izicelo eziphezulu zokuphuma.
Inye into yokugqibela ekufuneka uyiqwalasele ekufakeni imisonto - ukuba ilabhoratri yakho isebenzisa i-automation, unokufuna ukuqwalasela ukuba yeyiphi intambo enokusetyenziswa kunye nesixhobo sokubamba.
Umthamo Wokugcina
Iingqayi zeCryogenic zifumaneka ngokobukhulu obahlukeneyo ukugubungela uninzi lweemfuno, kodwa ubukhulu becala ziba phakathi komthamo we-1 mL kunye ne-5 mL.
Undoqo kukuqinisekisa ukuba i-cryovial yakho ayigcwaliswanga kwaye kukho igumbi elongezelelweyo elikhoyo, xa kunokwenzeka ukuba isampulu idumbe ngelixa ikhenkceza. Ngokwesiqhelo, oku kuthetha ukuba iilabhoratri zikhetha i-1 mL yeebhotile xa zigcina iisampulu ze-0.5 mL zeeseli ezixhonywe kwi-cryoprotectant, kunye ne-2.0 mL ye-1.0 mL yesampuli. Elinye icebiso lokungagcwalisi iibhotile zakho ngokugqithisileyo kukwenza ukuba usebenzise ii-cryovials ezineempawu ezithweswe izidanga, nto leyo eya kuqinisekisa ukuba uyakuthintela nakuphi na ukudumba okunokubangela ukuqhekeka okanye ukuvuza.
Screw Cap vs Flip Top
Uhlobo oluphezulu olukhethayo luxhomekeke ngokukodwa ekubeni uya kusebenzisa i-nitrogen yesigaba solwelo okanye hayi. Ukuba kunjalo, uya kufuna i-screw capped cryovials. Oku kuqinisekisa ukuba abakwazi ukuvula ngempazamo ngenxa yokuphatha kakubi okanye utshintsho lobushushu. Ukongeza, ii-screw caps zivumela ukufunyanwa lula kwiibhokisi ze-cryogenic kunye nokugcinwa okusebenzayo ngakumbi.
Nangona kunjalo, ukuba awusebenzisi i-nitrogen yenqanaba lolwelo kwaye ufuna umphezulu olungele ngakumbi ekulula ukuwuvula, ke i-flip top yeyona ndlela ingcono. Oku kuya kongela ixesha elininzi njengoko kulula kakhulu ukuyivula, nto leyo eyenza ukuba ibe luncedo ngakumbi kwimisebenzi ephezulu yokuhambisa kunye nezo zisebenzisa iinkqubo zebhetshi.
Ukhuseleko lwetywina
Eyona ndlela ingcono yokuqinisekisa isitywina esikhuselekileyo kukuqinisekisa ukuba ikepusi yakho ye-cryovial kunye nebhotile zombini zakhiwe ngento enye. Oku kuya kuqinisekisa ukuba ziyacutheka kwaye zande ngaxeshanye. Ukuba zenziwe kusetyenziswa izinto ezahlukeneyo, ngoko ziya kuncipha kwaye zande ngamazinga ahlukeneyo njengoko ubushushu buguquka, izikhewu ezikhokelayo kunye nokuvuza okunokwenzeka kunye nongcoliseko olulandela.
Ezinye iinkampani zibonelela ngeewasha ezimbini kunye neflange kwinqanaba eliphezulu lokhuseleko lwesampulu kwiicryovials ezinemisonto yangaphandle. I-O-Ring cryovials ithathwa njengeyona nto ithembekileyo kwii-cryovials ezifakwe ngaphakathi.
Iglasi vs iPlastiki
Ukukhusela kunye nokulula, iilabhoratri ezininzi ngoku zisebenzisa iplastiki, ngokuqhelekileyo i-polypropylene, endaweni yee-ampules zeglasi ezivaliweyo. Ii-ampole zeglasi ngoku zithathwa njengokhetho oluphelelwe lixesha njengoko ngexesha lenkqubo yokutywina ukuvuza kombhobho ongabonakaliyo kunokuvela, okuthi xa kunyibilikisiwe emva kokugcinwa kwinitrogen engamanzi kunokubangela ukuba kuqhume. Abazifanelanga iindlela zangoku zokuleyibheli, nto leyo engundoqo ekuqinisekiseni ukulandeleka kwesampulu.
UkuZimela vs Imizantsi eRounded
Iingqayi ze-Cryogenic zifumaneka zombini njengoko zizimele kunye namazantsi amile okwenkwenkwezi, okanye njengamazantsi angqukuva. Ukuba ufuna ukubeka iingqayi zakho phezu komhlaba ke qiniseka ukuba ukhethe ukuzimela
Ukulandelelwa kunye nokulandelela iSampuli
Le ndawo yokugcina i-cryogenic ihlala ingahoywa kodwa ukulandelwa kwesampulu kunye nokulandeleka kungumba obalulekileyo ekufuneka uqwalaselwe. Iisampulu ze-Cryogenic zinokugcinwa iminyaka emininzi, apho abasebenzi banokutshintsha kwaye ngaphandle kweerekhodi ezigcinwe ngokufanelekileyo ziyakwazi ukungabonakali.
Qinisekisa ukuba ukhetha iingqayi ezenza uchongo lwesampulu lube lula kangangoko. Izinto ekufuneka uziqwalasele ziquka:
Iindawo ezinkulu zokubhalela ukurekhoda iinkcukacha ezaneleyo ukuze iirekhodi zifunyanwe ukuba i-vial ibekwe kwindawo engachanekanga - ngokuqhelekileyo isazisi seseli, umhla owenziwe ngumkhenkce, kunye noonobumba bokuqala bomntu onoxanduva lwanele.
Iibhakhowudi ukunceda ulawulo isampuli kunye neenkqubo umkhondo
Iminqwazi enemibala
Inqaku lexesha elizayo - iitshiphusi ezikwaziyo ukumelana nokubanda kakhulu ziyaphuhliswa ezithi, xa zifakwe ngaphakathi kwi-cryovials nganye, zibenakho ukugcina imbali eneenkcukacha zobushushu kunye neenkcukacha zebhetshi ezineenkcukacha, iziphumo zovavanyo kunye namanye amaxwebhu afanelekileyo asemgangathweni.
Ukongeza ekuqwalaseleni iinkcukacha ezahlukeneyo zeengqayi ezikhoyo, enye ingcamango kufuneka inikwe kwinkqubo yobugcisa yokugcina i-cryovials kwinitrogen engamanzi.
Ubushushu boGcino
Kukho iindlela ezininzi zokugcina ukugcinwa kwe-cryogenic yeesampuli, nganye isebenza kwiqondo lokushisa elithile. Iinketho kunye nobushushu abasebenza kuzo ziquka:
Inqanaba lolwelo LN2: gcina ubushushu obuyi -196℃
Isigaba somphunga LN2: ziyakwazi ukusebenza kwiqondo elithile lobushushu phakathi kwe -135 ° C kunye -190 ° C ngokuxhomekeke kwimodeli.
Izikhenkcisi zomphunga wenitrogen: -20°C ukuya ku -150°C
Uhlobo lweeseli ezigcinwayo kunye nendlela yokugcina ekhethwa ngumphandi iya kugqiba ukuba yeyiphi kwezi zintathu iinketho ezikhoyo ezisebenzisayo ilabhoratri yakho.
Nangona kunjalo, ngenxa yobushushu obuphantsi kakhulu obusetyenziswayo ayizizo zonke iityhubhu okanye uyilo oluya kufaneleka okanye lukhuseleke. Izixhobo zinokuba brittle kakhulu kumaqondo obushushu aphantsi kakhulu, ukusebenzisa ivial engafanelanga ukusetyenziswa kwiqondo lobushushu obukhethileyo kunokubangela ukuba inqanawa yonakale okanye iqhekeze ngexesha lokugcinwa okanye ukunyibilika.
Jonga ngononophelo iingcebiso zabenzi bokusetyenziswa ngendlela eyiyo njengoko ezinye iingqayi ze-cryogenic zifanelekile kumaqondo obushushu asezantsi njenge-175°C, amanye -150°C amanye angama-80°C.
Kwakhona kuyafaneleka ukuba uqaphele ukuba abaninzi abavelisi bachaza ukuba izitya zabo ze-cryogenic azifanelekanga ukucwiliswa kwinqanaba le-liquid. Ukuba ezi ngqayi zigcinwe kwisigaba solwelo xa zibuyela kwiqondo lobushushu begumbi ezi ngqayi okanye i-cap seal yazo inokuqhekeka ngenxa yokwakheka ngokukhawuleza koxinzelelo olubangelwa kukuvuza okuncinci.
Ukuba iiseli ziza kugcinwa kwisigaba solwelo se-nitrogen engamanzi, cinga ngokugcina iiseli kwiingqayi ezifanelekileyo ze-cryogenic ezitywinwe ngobushushu kwityhubhu ye-cryoflex okanye ukugcina iiseli kwii-ampules zeglasi ezivalwe ngokwe-hermetically.
Ixesha lokuposa: Nov-25-2022