96-ipleyiti enzulu yequla (Icwecwe elinzulu) luhlobo lwepleyiti enamanzi amaninzi edla ngokusetyenziswa kwiilabhoratri. Inoyilo lomngxunya onzulu kwaye idla ngokusetyenziselwa imifuniselo efuna imiqulu emikhulu yeesampulu okanye iirejenti. Ezi zilandelayo zezinye zeendlela eziphambili zokusetyenziswa kunye neendlela zokusetyenziswa kweepleyiti zequla ezinzulu ezingama-96:
Uluhlu lwesicelo:
Ukuhlolwa kweziphumo eziphezulu: Kuvavanyo olunjengokuhlolwa kweziyobisi kunye nokuhlolwa kwethala leencwadi elihlanganisiweyo, iipleyiti zequla ezinzulu ezingama-96 zinokuthatha iisampulu ezininzi kwaye ziphucule ukusebenza kakuhle kovavanyo.
Ukukhuliswa kweeseli: Ilungele imifuniselo yenkcubeko yeseli efuna umthamo omkhulu wolwimi lwenkcubeko, ngakumbi inkcubeko yeeseli ezibambelelayo.
I-Enzyme-linked immunosorbent assay (ELISA): Isetyenziswe kwiimvavanyo ze-ELISA ezifuna umthamo omkhulu wenkqubo yokusabela.
Iimvavanyo zebhayoloji yemolekyuli: Ezinje nge-PCR reactions, DNA/RNA extraction, electrophoresis sample preparation, etc.
Ukubonakaliswa kweprotheyini kunye nokucocwa: Isetyenziswa kwimifuniselo eneprotein enkulu okanye ifuna umthamo omkhulu webuffer.
Ukugcinwa kwesampuli ixesha elide: Ngenxa yobunzulu bomngxuma omkhulu, ukuguqulwa komthamo wesampulu ngexesha lokukhenkceza kungancitshiswa, okulungele ukugcinwa kwexesha elide.
Indlela yokusetyenziswa:
Ukulungiselela isampulu: Ngokweemfuno zovavanyo, ukulinganisa ngokuchanekileyo isixa esifanelekileyo sesampulu okanye i-reagent kwaye uyongeze kwiqula lepleyiti yequla elinzulu.
Ukutywina: Sebenzisa ifilimu yokutywina efanelekileyo okanye igasket ukutywina ipleyiti yomthombo ukunqanda ukuvela komphunga okanye ukosuleleka.
Ukuxuba: Gcoba ngobumnene okanye usebenzise i-pipette ye-multichannel ukuxuba isampuli ukuqinisekisa ukuba isampuli idibene ngokupheleleyo ne-reagent.
Ukufukama: Beka ipleyiti enzulu kwiqula lobushushu elingaguqukiyo okanye enye indawo efanelekileyo yokufukamela ngokweemfuno zovavanyo.
Ukufunda idatha: Sebenzisa izixhobo ezifana ne-microplate readers kunye ne-fluorescence microscopes ukufunda iziphumo zovavanyo.
Ukucoca kunye nokubulala iintsholongwane: Emva kovavanyo, sebenzisa izicoci ezifanelekileyo ukucoca ipleyiti enzulu kwaye ubulale iintsholongwane.
Ugcino: Ipleyiti enzulu yequla kufuneka igcinwe ngokufanelekileyo emva kokucoca kunye nokubulala iintsholongwane ukuthintela ukosuleleka.
Xa usebenzisa iipleyiti zequla ezinzulu ezingama-96, kufuneka kuqatshelwe la manqaku alandelayo:
Iinkcukacha zokusebenza: Landela imilinganiselo yokusebenza kwe-aseptic ukunqanda ungcoliseko lwesampulu.
Ukuchaneka: Sebenzisa i-pipette ye-multichannel okanye inkqubo yokuphatha ulwelo oluzenzekelayo ukuphucula ukuchaneka komsebenzi.
Ukumakishwa okucacileyo: Qinisekisa ukuba iqula ngalinye lepleyiti yomthombo liphawulwe ngokucacileyo ukuze libonwe lula kwaye lirekhodwe.
96-unzulu kakuhleiipleyiti sisixhobo esibalulekileyo kwimifuniselo ephezulu kwilabhoratri. Ukusetyenziswa ngokufanelekileyo kunokuphucula kakhulu ukusebenza kunye nokuchaneka kovavanyo.
Ixesha lokuposa: Aug-13-2024