I-96-ne-vere elungileyo yeplate (Ipleyiti enzulu) Luhlobo lwepleyiti enezinto ezininzi ezisetyenziswa kakuhle kwilebhu. Inoyilo lomngxunya onzulu kwaye ihlala isetyenziselwa uvavanyo olufuna imiqulu emikhulu yeesampulu okanye iirejista. Oku kulandelayo zezinye zezona rangs zendlela ephambili kunye neendlela zokusetyenziswa ze-96-pite ezinzulu ze-96.
Uluhlu lwesicelo:
Uvavanyo lwegalelo eliphezulu: kwiimvavanyo ezinjengokuvavanywa kweziyobisi kunye nokuhlolwa kwethala leencwadi ezingama-96-i-96 - iipleyiti ezinzulu ezingama-96 zinokufumana iisampulu ezininzi kunye nokuphucula ukusebenza kakuhle.
Inkcubeko yeselfowuni: Ifanelekile kwiimvavanyo zenkcubeko ezifuna ivolumu enkulu yenkcubeko, ngakumbi inkcubeko yeeseli ezihlelelekileyo.
I-Enzyme-I-Innosorbent Assay Assay (Elisa): Isetyenziswe kwiimvavanyo ze-Elisa ezifuna ivolumu enkulu yenkqubo yokuphendula.
I-molecular bialogy: njengokuphendula kwe-PCR, i-DNA / Ukukhutshwa kwe-DNA / iRNA, i-elektrophoressis isampulu yesampulu, njl.
Iprotein kunye nokuCoca: Isetyenziselwa iimvavanyo ezibonisa intetho enkulu yeprotein okanye ifuna ivolumu enkulu ye-buffer.
Ukugcinwa kwesampulu yethutyana yexesha elide: Ngenxa yobunzulu bendlela enkulu, ukutshintsha kwevolumu yesampulu ngexesha lokukhululeka kunokuncitshiswa, nto leyo efanelekileyo ukugcinwa kwexesha elide.
Indlela yokusetyenziswa:
Ukulungiselela kwesampulu: ngokweemfuno zovavanyo, ukulinganisa ngokuchanekileyo isixa esifanelekileyo sesampulu okanye ukuqalisa kwaye yongeze kwiqula lenzululwazi enzulu.
Ukutywina: Sebenzisa ifiliki efanelekileyo yokutywina okanye i-gasket yokutywina ipleyiti yequla ukuthintela isampulu okanye ungcoliseko.
Ukuxuba: Ngomsindo shake umbhobho we-multikhannel ukuxuba isampulu ukuqinisekisa ukuba isampulu inxibelelana ngokupheleleyo ne-regent.
I-Incubation: Beka ipleyiti enzulu kwibhokisi yobushushu obuqhubekayo okanye enye indawo efanelekileyo yokufakwa kwemveliso ngokweemfuno zovavanyo.
Idatha yokufunda: Sebenzisa izixhobo ezinje ngo-Mulflate kunye ne-microscopes ye-fluoresces yokufunda iziphumo zovavanyo.
Ukucocwa kunye nokuzibulala
Ukugcinwa: Ipleyiti enenzululwazi enzulu kufuneka igcinwe ngokufanelekileyo emva kokucoca kunye nokubulala iintsholongwane ukunqanda ungcoliseko.
Xa usebenzisa i-96 - iipleyiti ezinzulu kakhulu, ezi ngongoma zilandelayo kufuneka ziqatshelwe:
Ukucaciswa kwemisebenzi: Landela imigaqo-nkqubo yokusebenza ye-ASEptic ukuthintela iSampulu ungcoliseko lwesampulu.
Ukuchaneka: Sebenzisa i-pipette ye-multictte okanye inkqubo yokuphatha amanzi okuzenzekelayo yokuphucula ukuchaneka komsebenzi.
Ukumakisha okucacileyo: Qiniseka ukuba iqula elinye lequla eliphawulwe ngokucacileyo ukuba ichongwe ngokulula kunye nokurekhoda.
I-96-inzulu kakhulu-kakuhleIipleyiti sisixhobo esibalulekileyo sovavanyo oluninzi lokungena kwilabhoratri. Ukusetyenziswa ngokufanelekileyo kunokuphucula kakhulu ukusebenza nokuchaneka kovavanyo.
IXESHA LOKUQALA: Aug-13 ukuya ku-1224