96-ipleyiti enzulu yequla (Icwecwe elinzulu) luhlobo lwepleyiti enamanzi amaninzi edla ngokusetyenziswa kwiilabhoratri. Inoyilo lomngxuma onzulu kwaye idla ngokusetyenziselwa imifuniselo efuna imiqulu emikhulu yeesampulu okanye ii-reagents. Ezi zilandelayo zezinye zeendlela eziphambili zokusetyenziswa kunye neendlela zokusetyenziswa kweepleyiti zequla ezinzulu ezingama-96:
Uluhlu lwesicelo:
Ukuhlolwa kweziphumo eziphezulu: Kuvavanyo olunjengokuhlolwa kweziyobisi kunye nokuhlolwa kwethala leencwadi elihlanganisiweyo, iipleyiti zequla ezinzulu ezingama-96 zinokuthatha iisampulu ezininzi kwaye ziphucule ukusebenza kakuhle kovavanyo.
Ukukhuliswa kweeseli: Ilungele imifuniselo yenkcubeko yeseli efuna umthamo omkhulu wolwimi lwenkcubeko, ngakumbi inkcubeko yeeseli ezibambelelayo.
I-Enzyme-linked immunosorbent assay (ELISA): Isetyenziswe kwiimvavanyo ze-ELISA ezifuna umthamo omkhulu wenkqubo yokusabela.
Iimvavanyo zebhayoloji yemolekyuli: Ezinje nge-PCR reactions, DNA/RNA extraction, electrophoresis sample preparation, etc.
Ukubonakaliswa kweprotheyini kunye nokucocwa: Isetyenziswa kwimifuniselo eneprotein enkulu okanye ifuna umthamo omkhulu webuffer.
Ukugcinwa kwesampuli ixesha elide: Ngenxa yobunzulu bomngxuma omkhulu, ukuguqulwa komthamo wesampulu ngexesha lokukhenkceza kungancitshiswa, okulungele ukugcinwa kwexesha elide.
Indlela yokusetyenziswa:
Ukulungiselela isampulu: Ngokweemfuno zovavanyo, ukulinganisa ngokuchanekileyo isixa esifanelekileyo sesampulu okanye i-reagent kwaye uyongeze kwiqula lepleyiti yequla elinzulu.
Ukutywina: Sebenzisa ifilimu yokutywina efanelekileyo okanye igasket ukutywina ipleyiti yomthombo ukunqanda ukuvela komphunga okanye ukosuleleka.
Ukuxuba: Gcoba ngobumnene okanye usebenzise i-pipette ye-multichannel ukuxuba isampuli ukuqinisekisa ukuba isampuli idibene ngokupheleleyo ne-reagent.
Ukufukama: Beka ipleyiti enzulu kwiqula lobushushu elingaguqukiyo okanye enye indawo efanelekileyo yokufukamela ngokweemfuno zovavanyo.
Ukufunda idatha: Sebenzisa izixhobo ezifana ne-microplate readers kunye ne-fluorescence microscopes ukufunda iziphumo zovavanyo.
Ukucoca kunye nokubulala iintsholongwane: Emva kovavanyo, sebenzisa izicoci ezifanelekileyo ukucoca ipleyiti enzulu kwaye ubulale iintsholongwane.
Ugcino: Ipleyiti enzulu yequla kufuneka igcinwe ngokufanelekileyo emva kokucoca kunye nokubulala iintsholongwane ukuthintela ukosuleleka.
Xa usebenzisa iipleyiti zequla ezinzulu ezingama-96, kufuneka kuqatshelwe la manqaku alandelayo:
Inkcazo yokusebenza: Landela inkcazo yokusebenza kwe-aseptic ukunqanda ungcoliseko lwesampulu.
Ukuchaneka: Sebenzisa i-pipette ye-multichannel okanye inkqubo yokuphatha ulwelo oluzenzekelayo ukuphucula ukuchaneka komsebenzi.
Ukumakishwa okucacileyo: Qinisekisa ukuba iqula ngalinye lepleyiti yomthombo liphawulwe ngokucacileyo ukuze libonwe lula kwaye lirekhodwe.
96-unzulu kakuhleiipleyiti sisixhobo esibalulekileyo kwimifuniselo ephezulu kwilabhoratri. Ukusetyenziswa ngokufanelekileyo kunokuphucula kakhulu ukusebenza kunye nokuchaneka kovavanyo.
Ixesha lokuposa: Aug-13-2024