I-micropipette mhlawumbi sesona sixhobo sisetyenziswa kwilabhoratri. Zisetyenziswa zizazinzulu kuluhlu olubanzi lwamacandelo abandakanya iziko lemfundo, izibhedlele kunye neelebhu ze forensics kunye nophuhliso lwamachiza kunye nogonyo ukudlulisa ngokuthe ngqo, izixa ezincinci kakhulu zolwelo.
Ngelixa kunokucaphukisa kwaye kuyakhathaza ukubona amaqamza omoya kwincam yepipette elahlwayo ukuba ayibonwa okanye ayihoywa inokuba nempembelelo enkulu ekuthembekeni nasekuphindaphindeni iziphumo.
Iindaba ezimnandi zezokuba kukho amanyathelo alula onokuthi uwathathe ukuthintela amaqamza omoya, nokuphucula ukusebenza kakuhle kwelebhu, ukwaneliseka kwabaqhubi kunye nokuchaneka nokuchaneka kweziphumo.
Ngezantsi, siphonononga isiphumo sokufumana iqamza lomoya kwincam yepipette yakho kunye nento omawuyenze ngokulandelayo.
Isiphumo seBubbles kwiIngcebiso kaPipette
Nangona usebenzisa i-pipettes echanekileyo, ephezulu, egcinwe kakuhle, enenkonzo kunye nokulinganisa ukuthembeka kweziphumo zakho kunokuchaphazeleka kwiimpazamo zelebhu. Xa amaqamza engena kwiingcebisoinokuba neziphumo ezininzi.
● Xa umsebenzisi ebona iqamza lomoya kufuneka achithe ixesha ekhupha ulwelo olubasiweyo ngokufanelekileyo, akhuphe incam aze aqalise inkqubo kwakhona.
● Amaqamza omoya angabonakaliyo angabangela ukuhanjiswa kwevolumu ephantsi, ngaloo ndlela ukuguqula ukuxinwa kweengxube zokuphendula ezikhokelela kwizilingo ezingaphumelelanga kunye neziphumo ezithandabuzekayo okanye ezingathembekiyo.
Ezi ziphumo zinokuba neziphumo ezininzi (1).
● UKucutheka kokuSebenza kweLebhu – Iimvavanyo kunye novavanyo kuya kufuneka ziphindaphindwe, kube neendleko zabasebenzi kunye nezixhobo, ezinokuthi zibe nkulu kakhulu.
● Iziphumo zoVavanyo ezithandabuzekayo okanye ezingachanekanga - Ukuba iziphumo ezingachanekanga zikhutshwe kunokubakho iziphumo ezibi kakhulu ezibandakanya ukuxilonga kunye neziphumo ezibi zesigulane.
● Ukurhoxiswa kweMibhalo esuka kwiiJenali - Ukuba oontanga bayasilela ukuphinda iziphumo zakho ngenxa yamaqamu omoya abangela ukuba amaphepha eziphumo ezingachanekanga arhoxiswe.
IiNgcebiso eziGqwesileyo zokuThintela amaqamza oMoya
Kwiimeko ezininzi iibhubhu zomoya kwiingcebiso ze-pipette zibangelwa yimpazamo yomsebenzisi. Ubuchule obugwenxa ngenxa yoqeqesho olunganelanga okanye ukudinwa kudla ngokuba yeyona ngxaki iphambili.
Ukubetha ngemibhobho ngumsebenzi wezakhono ofuna ingqwalasela eyi-110%, uqeqesho olululo kunye nokuziqhelanisa nokufikelela kwiziphumo ezingaguqukiyo nezichanekileyo.
Ngelixa kukho izinto ezininzi onokuthi uzenze ukunciphisa iimpazamo zombhobho jikelele, ngezantsi siphawule ezona ndlela zilungileyo ezinokusetyenziswa ukunqanda amaqamza omoyaiingcebiso zepipette.
Phucula iNdlela yoMsebenzisi
Pipette Kancinci
Ukuba i-plunger ikhutshwe ngokukhawuleza xa inqwenela, amaqamza omoya anokwaziswa kwincam. Oku kunokuba yingxaki ngakumbi xa uhambisa ulwelo lwe-viscous. Isiphumo esifanayo singenzeka ukuba iplunger ikhululwe ngokukhawuleza emva kokukhupha.
Ukuze ugweme amaqamza omoya xa unqwenela, qaphela ukuba usebenze ipiston yeepipettes ze-manual ngendlela egudileyo kunye neqhelekileyo, usebenzisa amandla angaguqukiyo.
Sebenzisa Ubunzulu bokuntywiliselwa obuchanekileyo
Ukungaphumeleli ukuntywilisela incam ye-pipette enzulu ngokwaneleyo ngaphantsi kwe-meniscus ye-liquid reservoir kunokubangela ukulangazelela umoya kwaye ngaloo ndlela ukubunjwa kwe-bubble.
Nangona kunjalo, ukuntywiliselwa incam enzulu kakhulu kunokunqwenela ulwelo oluninzi ngenxa yoxinzelelo olongezelelweyo okanye amathontsi anokuthi enzeke ngaphandle kwencam ngoko ke kubalulekile ukuntywilisela.incam yepipettekubunzulu obuchanekileyo.
Ubunzulu obucetyiswayo buhluka phakathi kobukhulu bepipette, uhlobo kunye nokwenza. Ngelixa iingcebiso zomenzi kufuneka zilandelwe nasi isikhokelo esibonelelwa yiNational Physical Laboratory.
Isikhokelo ukuya kubunzulu bokuntywiliselwa kwengcebiso
Umthamo wePipette (µl) kunye nobunzulu boMbizo (mm)
- 1 – 100: 2 – 3
- 100 – 1,000: 2 – 4
- 1,000 - 5,000: 2 - 5
Phambi-KumanziIingcebiso zePipette
Xa imibhobho imithamo emikhulu kune-10µliingcebiso zepipettezivame ukumanziswa kwangaphambili ngokuzalisa amaxesha amaninzi kunye nolwelo olukhutshwayo kwaye lugxothwe ukuba luchithe ukuphucula ukuchaneka.
Ukungaphumeleli ukumanzisa kwangaphambili kunokubangela amaqamza omoya, ngakumbi xa usebenzisa i-viscous okanye i-hydrophobic liquids. Ukunqanda amaqamza omoya qinisekisa ukuba uneengcebiso zokumanzisa kwangaphambili xa umthamo wemibhobho ungaphezulu kwe-10µl.
Sebenzisa uBuchule boBuchule boMbhobho ukuba bufanelekile
I-Viscous Substances: Ingxaki eqhelekileyo xa i-pipetting viscous substances ezifana ne-protein okanye i-nucleic acids solutions, i-glycerol kunye ne-Tween 20/40/60/80 kukubunjwa rhoqo kwamaqamza xa kusetyenziswa ubuchule bokuhamba phambili kwemibhobho.
Ukufakela imibhobho ngokucothayo, ukusebenzisa ubuchule bokubuyisela umva umbhobho kunciphisa umngcipheko wokwakheka kweqamza xa kuhanjiswa izisombululo ze-viscous.
ELISA Technique
I-reverse pipetting iyacetyiswa xa ufaka imibhobho emincinci emincinci96 kakuhle micro test iipleyitikubuchule be-ELISA. Xa iibhubhu zomoya zitsalwa kwi-pipette okanye zichithwa kwimithombo xa zongeza i-reagents zinokuchaphazela ixabiso le-optical density kunye neziphumo. I-reverse pipetting iyacetyiswa ukunciphisa okanye ukuphelisa lo mbandela.
Sebenzisa i-Ergonomic Pipettes
Iipayipi zesitayile esidala ezingakhange ziyilwe nge-ergonomics engqondweni zifuna umgudu othe kratya womzimba, uyadinwa kwaye ubuchule bakho bokubhobhoza buba bubucukubhede kwaye buhlwempuzeke. Iimpazamo ezikhankanywe ngasentla ezifana nokukhupha iplunger ngokukhawuleza zinokuthi zenzeke rhoqo.
Ngokutyala imali kwisisombululo se-ergonomic ngakumbi uya kukwazi ukugcina ubuchule obugqwesileyo kwaye uthintele ukwakheka kwamaqamza omoya ngenxa yobuchule obugwenxa.
Thatha ixesha lokuqeqesha abasebenzi
Ukuqeqeshwa rhoqo kunye nokuvavanya abasebenzi kwiindlela zokusebenzisa imibhobho kunokuqinisekisa ukuba impazamo yabaqhubi kunye nokwakheka kwe-bubble yomoya kuyancitshiswa.
Qwalasela ngakumbi Izisombululo eziziSebenzisayo
Njengoko kuphawuliwe ngasentla amaqamza omoya amaninzi abangelwa ngumsebenzi. Kunokwenzeka ukunciphisa imposiso kunye nentuthuzelo yabaqhubi ngokusebenzisa iipayipi ze-elektroniki okanye iqonga lokuphatha ulwelo oluguquguqukayo njengeI-Agilent Bravo Liquid Handing Robot.
Sebenzisa uMgangatho OlungileyoIingcebiso zePipette
I-Micropipettes idla ngokuthengwa ngononophelo, kodwa kaninzi ingcamango encinci inikezelwa kumgangatho we-pipette elahlayo. Ngenxa yempembelelo yokuba i-tip ineziphumo zombhobho, umgangatho we-ISO 8655 ufuna ukulinganisa okongeziweyo ukuba iipayipi kunye neengcebiso ezivela kubakhiqizi abahlukeneyo zisetyenziswa.
Oku kungenxa yokuba iingcebiso ezininzi ezinexabiso eliphantsi zinokujongeka kakuhle ekuqaleni kodwa xa uzifundisisa ngononophelo zisenokuba nemibane, iiprotrusions, imikrwelo, namaqamza omoya, okanye zigobe okanye ziqulathe ubumdaka.
Ukuthenga iingcebiso zomgangatho olungileyo ezenziwe ngepolypropylene yodidi oluphezulu kunokunciphisa ukwenzeka kwamaqamza omoya.
Ukushwankathela
Ukufumana amaqamza omoya kwincam yepipette yakho kunempembelelo ekusebenzeni kakuhle kwelebhu kunye nokungachaneki nokungachaneki kweziphumo. Siqaphele izinto ezininzi onokuzenza ukunqanda amaqamza omoya ukuba angeneincam yepipette.
Nangona kunjalo, ukuba umgangatho ophantsiiingcebiso zepipettezibangela amaqamza omoya ukuba angene kwincam yepipette yakho, uya konwatyiswa kukwazi ukuba ukulunga kwethu jikelele.iingcebiso zepipettezenziwe ngemigangatho ephezulu kwaye zenziwe nge-premium-grade pure polypropylene.
Inkampani yaseSuzhou Ace Biomedicalukuvelisa umgangatho ophezulu we-10,20,50,100,200,300,1000 kunye ne-1250 µL imithamo yeengcebiso zepipette jikelele, iingcebiso ezingama-96/irack. Ukuqina okubalaseleyo - zonke iirakhi zeengcebiso ze-ACE zimelane neemfuno zokusetyenziswa kunye neepipetttors ezininzi. Inyumba, iFilter, i-RNase-/DNase-free, kunye ne-nonpyrogenic.
Wamkelekile ukuba usibuze ngeenkcukacha ezithe vetshe.
Ixesha lokuposa: Dec-29-2022