Ukuhlelwa kweengcebiso zepipette zebhubhoratri
zinokwahlulwa zibe zezi ntlobo zilandelayo: Iingcebiso eziqhelekileyo, iingcebiso zokucoca, iingcebiso ezinqwenelekayo eziphantsi, iingcebiso zeendawo zokusebenza ezizenzekelayo kunye neengcebiso ezibanzi. Yilabhoratri esetyenziswayo engasetyenziswa ngokubambisana nepipette. Isetyenziswa ikakhulu kwiimeko ezahlukeneyo zemibhobho.
1.Iingcebiso zePipette jikelele
Iingcebiso ze-Universal Pipette zizona ngcebiso ezisetyenziswa kakhulu, ezingasetyenziselwa phantse yonke imisebenzi yokubhobhoza, kwaye zikwayeyona ndlela yoqoqosho yeengcebiso. Ngokubanzi, iingcebiso ezisemgangathweni zinokugubungela uninzi lwemisebenzi yombhobho. ezinye iindidi zeengcebiso ziye zavela kwiingcebiso eziqhelekileyo. Ngokuqhelekileyo kukho iindlela ezininzi zokupakisha kwiingcebiso eziqhelekileyo, kwaye kukho iintlobo ezintathu eziqhelekileyo kwiimarike: kwiingxowa, kwiibhokisi, nakwiipleyiti ezifakwe ngaphambili (zipakishwe).
Xa abasebenzisi beyisebenzisa, ukuba baneemfuno ezikhethekileyo zokuvala inzala, banokuthenga iibhokisi ezinezintsholongwane ngokuthe ngqo. , okanye ubeke iingcebiso zesingxobo esingasulungekanga kwibhokisi yencam engenanto ukuze uzibulale phambi kokusetyenziswa.
2.Iingcebiso ezihluziweyo
Iingcebiso ezihluziweyo zisetyenziselwa ukuthintela usulelo olunqamlezayo. Isampuli ethathwe yi-tip yokucoca ayikwazi ukungena ngaphakathi kwi-pipette, ngoko ke iindawo ze-pipette zikhuselwe ekungcoleni kunye nokubola. Okubaluleke ngakumbi, kunokuqinisekisa ukuba akukho ngcoliseko phakathi kweesampulu kwaye isetyenziswa ngokubanzi kuvavanyo olufana nebhayoloji yemolekyuli, i-cytology kunye neentsholongwane.
3.Iingcebiso zePipette zokugcinwa okuphantsi
Kwimifuniselo efuna ubuntununtunu obuphezulu, okanye iisampulu ezixabisekileyo okanye ii-reagents ezithambekele kwiintsalela, unokukhetha iingcebiso eziphantsi ze-adsorption ukuphucula ukubuyiswa. Kukho iimeko apho ngaphezulu kushiywe. Kungakhathaliseki ukuba uluphi uhlobo lwengcebiso oyikhethayo, ireyithi yentsalela ephantsi ingundoqo.
Ukuba siqwalasela ngokucophelela inkqubo yokusetyenziswa kwesicatshulwa, siya kufumanisa ukuba xa i-liquid ikhutshwe, kukho rhoqo inxalenye engenakuchithwa kwaye ihlala kwi-tip. Oku kwazisa impazamo ethile kwiziphumo nokuba loluphi umfuniselo owenziweyo. Ukuba le mpazamo ingaphakathi kuluhlu olwamkelekileyo, usenokukhetha ukusebenzisa i-prompts eqhelekileyo.Ukuba siqwalasela ngokucophelela inkqubo yokusetyenziswa kwengcebiso, siya kufumanisa ukuba xa ulwelo lukhutshwa, kuhlala kukho inxalenye engenakukhuculwa kwaye ihlale. kwincam. Oku kwazisa impazamo ethile kwiziphumo nokuba loluphi umfuniselo owenziweyo. Ukuba le mpazamo ikuluhlu olwamkelekileyo, usenokukhetha ukusebenzisa imiyalelo eqhelekileyo.
4.Iingcebiso ze-Robotic Pipette
Indawo yokusebenzela incam ihambelana ikakhulu nendawo yokusebenzela engamanzi, enokubona inqanaba lolwelo kwaye iqinisekise ukuchaneka kombhobho. Iipipettes eziphezulu ezisetyenziswa ngokuqhelekileyo kwi-genomics, i-proteomics, i-cytomics, i-immunoassay, i-metabolomics, uphando lwe-biopharmaceutical kunye nophuhliso, njl. Iindawo zokusebenzela zezi ntlobo zintlanu ziphantse zalawula ishishini lonke.
5. Iingcebiso zepipette yomlomo obanzi
Iingcebiso ezibanzi zomlomo zifanelekile kwi-pipetting viscous materials, i-genomic DNA, kunyeInkcubeko yeSeliulwelo; ziyahluka kwiingcebiso eziqhelekileyo ngokuba nevula enkulu emazantsi ukuze kube lula ukuchithwa kunye neendlela ezincinci. ukusika. Xa kufakwe umbhobho wezinto ezibonakalayo, intloko yokufunxa yemveli inendawo encinci yokuvula ezantsi, ekungekho lula ukuyichola kunye nokuvuza, kwaye ibangela intsalela ephezulu. Uyilo oluvuthayo luququzelela ukuphathwa kweesampuli ezinjalo.
Ebusweni be-DNA ye-genomic kunye neesampuli zeeseli ezibuthathaka, ukuba ukuvulwa kuncinci kakhulu, kulula ukulimaza isampuli kwaye kubangele ukuphuka kweeseli ngexesha lokusebenza. Iingcebiso zexilongo ezinokuvulwa okumalunga ne-70% enkulu kuneengcebiso ezisemgangathweni zilungile kwiisampulu eziethe-ethe zemibhobho. Isisombululo esihle kakhulu.
Ixesha lokuposa: Dec-10-2022