Kummandla wophando lwezenzululwazi, ngakumbi kwiinkalo ezifana nebhayoloji, ibhayoloji yeseli, kunye nekhemesti, ukhetho lwezixhobo zaselabhoratri lunokuchaphazela kakhulu ukusebenza kunye nokuchaneka kovavanyo. Esinye eso sigqibo sibalulekileyo kukukhethwa phakathi kwamaqula angama-96 kunye namaqula angama-384. Zombini ezi ntlobo zepleyiti zineesethi zazo zoncedo kunye nezinto ezinokuthi zibe khona. Isitshixo sokuphucula ukusebenza kakuhle kwelebhu silele ekuqondeni lo mahluko kunye nokukhetha eyona ifanele iimfuno ezithile zovavanyo.

1. Umthamo kunye noPhuculo

Olunye ulwahlulo oluphambili phakathi kwe-96-well kunye ne-384-well-plate linani lamaqula, elichaphazela ngokuthe ngqo umthamo we-reagents ezinokuthi zisetyenziswe kunye ne-throughput of experiments. Ipleyiti enamanzi angama-96, enemingxuma emikhulu, ibamba umthamo othe kratya, iyenze ilungele uvavanyo olufuna iirejenti ezingaphezulu okanye iisampulu, kunye nemifuniselo apho ukuphuphuma komphunga kunokuba yinkxalabo. Ngokuchaseneyo, iipleyiti ezinamaqula angama-384, kunye noxinzelelo lwazo oluphezulu lwamaqula, zivumela inani elikhulu leemvavanyo ngaxeshanye, ngaloo ndlela kwandisa umthamo kakhulu. Oku kunenzuzo ngakumbi kwizicelo ze-high-throughput screening (HTS), apho ukukwazi ukucubungula amanani amaninzi eesampuli ngokukhawuleza kubaluleke kakhulu.

2. Ukusebenza kakuhle kweendleko

Iindleko yenye into ebalulekileyo ekufuneka iqwalaselwe. Ngelixa iipleyiti ezingama-384 zihlala zivumela iimvavanyo ezininzi ngepleyiti nganye, ezinokunciphisa iindleko zovavanyo ngalunye, zinokufuna izixhobo zokuphatha ulwelo ezichaneke ngakumbi kwaye zihlala zibiza kakhulu. Ukongeza, imiqulu emincinci ye-reagent esetyenziswa kwiipleyiti zequla ezingama-384 inokukhokelela kugcino lweendleko ezinkulu kwii-reagents ngokuhamba kwexesha. Nangona kunjalo, iilebhu kufuneka zilungelelanise olu londolozo notyalo-mali lokuqala kwizixhobo eziphucukileyo.

3. Uvakalelo kunye noMgangatho weDatha

Uvakalelo lweemvavanyo ezenziwe kwi-96-well xa kuthelekiswa ne-384-well plates nazo zinokwahluka. Ngokuqhelekileyo, umthamo omkhulu kwiipleyiti zequla ezingama-96 unokunceda ukunciphisa ukuguquguquka kunye nokuphucula ukuphindaphinda kweziphumo. Oku kubenza balungele imifuniselo apho ukuchaneka kubaluleke kakhulu. Kwelinye icala, iipleyiti ezingama-384, ezinemithamo emincinci, zinokunyusa ubuntununtunu kwiimvavanyo ezithile, ezifana ne-fluorescence okanye i-luminescence-based assays, ngenxa yoxinzelelo oluphezulu lwesiginali.

4. Ukusetyenziswa kwendawo

Indawo yelabhoratri idla ngokuba yiprimiyamu, kwaye ukhetho lwepleyiti lunokuba nefuthe kwindlela esi kusetyenziswa ngayo ngokufanelekileyo esi sithuba. Amacwecwe angama-384 amaqula enza ukuba uvavanyo luqhutywe kwindawo efanayo xa kuthelekiswa neepleyiti ezingamaqula angama-96, ngokwandisa ibhentshi yelabhu kunye nesithuba se-incubator. Oku kunokuba luncedo ngakumbi kwiilebhu ezinendawo enyiniweyo okanye apho imisebenzi yophumezo oluphezulu iyimfuneko.

5. Ukuhambelana kweZixhobo

Ukuhambelana nezixhobo zaselebhu ezikhoyo yenye into ebalulekileyo ekufuneka iqwalaselwe. Iilabhoratri ezininzi sele zinezixhobo ezilungiselelwe iipleyiti ezinamaqula angama-96, ukusuka kwiirobhothi ezifaka imibhobho ukuya kwiipleyiti zokufunda. Ukutshintshela kwiipleyiti ezinamaqula angama-384 kunokufuna izixhobo ezitsha okanye uhlengahlengiso kwiinkqubo esele zikho, ezinokubiza imali eninzi kwaye zidle ixesha. Ke ngoko, iilebhu kufuneka zivavanye ngononophelo ukuba ngaba izibonelelo zokutshintshela kwiipleyiti zamaqula angama-384 ziyodlula le mingeni inokubakho.

Ukuqukumbela

Ekugqibeleni, isigqibo phakathi kokusebenzisa iipleyiti zequla ezingama-96 okanye ezingama-384 sixhomekeke kwiimfuno ezithile zelabhoratri kunye nohlobo lovavanyo oluqhutywayo. Kwimifuniselo efuna umthamo omkhulu kwaye apho ubuntununtunu kunye nokuveliswa kwakhona kubaluleke kakhulu, iipleyiti zamaqula angama-96 zinokuba lolona khetho lungcono. Ngokwahlukileyo, kwizicelo eziphezulu kunye nokusebenza kakuhle kweendleko ngokwemigaqo yokusetyenziswa kwe-reagent, iipleyiti zequla ezingama-384 zinokuphucula kakhulu ukusebenza kwelabhoratri. Iilabhoratri zimele zizihlolisise ngenyameko ezi nkalo, ziqwalasela iimeko zazo ezikhethekileyo, ukuze zenze olona khetho lunolwazi nolunempumelelo.