Kwindawo yophando lwezenzululwazi, ngakumbi kwimimandla efana ne-biochemistry, i-biology yeseli, kunye ne-pharmacology, ukhetho lwezixhobo zelebhu lungayichaphazela kakhulu impumelelo kunye nokuchaneka kovavanyo. Esona sigqibo sibalulekileyo kukhetho phakathi kwe-96-ne-384 -iplate. Zombini iintlobo zeplate zineeseti zazo zeenzuzo kunye neendlela ezinokubakho. Eyona nto iphambili yokwenza i-lab ye-LEB i-Lab ilele ekuqondeni la mahluko kwaye ukhetha eyona ilungele iimfuno ezithile zovavanyo.

1. Ivolumu kunye negalelo

Elinye lamahluko okuqala phakathi kwe-96-ne-384 -ipeyinti ezingama-384 linani lequla, elinefuthe ngqo kumthamo weerejista ezinokusetyenziswa kunye nokufakelwa kwezilingo. Iplate ye-96-kunye nequla elikhulu, eliqhelekileyo libambe ivolumu engaphezulu, eyenza ifanelekile ukuba ifuneke i-assays okanye iisampulu ezinokubakho apho inkxalabo enokuba yinkxalabo. Kwelinye icala, iipleyiti ezingama-384, kunye nokuxinana kwazo kwezilwanyana, zivumele inani elikhulu le-assus ngaxeshanye, ngaloo ndlela zanda kakhulu. Oku kuyingenelo ikakhulu kwiZicelo zokuHlola eziPhezulu (HTS), apho amandla okwenza iinombolo ezinkulu zeesampulu zibalulekile.

2. Ukusebenza kweendleko

Ixabiso lenye into ebalulekileyo ekufuneka uyiqwalasele. Ngelixa i-384 -ipeyinti ezihle zihlala zivumela iindlela ezingaphezulu kwiplate nganye, ezinokunciphisa iindleko kwi-ASSEAY nganye, zinokufuna izixhobo zokugcina izinto ezingaphantsi komhlaba. Ukongeza, imiqulu emincinci esetyenziswa kwiipleyiti ezingama-384 ingakhokelela kulondolozo lweendleko ezibalulekileyo kwiiRegents ngokuhamba kwexesha. Nangona kunjalo, iilebhu kufuneka zilungelelanise ezi mgcino kutyalo mali lokuqala kwizixhobo eziphambili.

I-3. Ubuntununtunu kunye nomgangatho wedatha

Imvakalelo ye-assay eyenziwe nge-96-ngokuchaseneyo ne-384 -ipeyinti kakuhle ezingama-384 nazo zahluka. Ngokubanzi, ivolumu enkulu kwiipleyiti ezingama-96, inokunceda ukunciphisa ukwahluka kwaye kuphuculwe ukuzala kweziphumo. Oku kubangela ukuba balungele uvavanyo apho kuchanekile khona. Kwelinye icala, iipleyiti ezingama-384, ezinamagqabi amancinci, zinokunyusa ubuntununtunu kwindawo ethile, enjengokufana okanye ukususwa kwe-fluorescence okanye i-assulelonescence esekwe kuyo, ngenxa yokuxinana okuphezulu.

I-4. Ukusetyenziswa kwendawo

Indawo yelebhu ihlala kwipremiyamu, kwaye ukhetho lweplate lunokuba nefuthe ekusetyenzisweni ngokukuko okusebenzayo. Iipleyiti ezingama-384 zenza ukuba izikhuselo ezininzi ziqhutywa kwindawo efanayo yomzimba xa kuthelekiswa neepleyiti ezingama-96, zandisa ngokufanelekileyo i-Lab Bench Bonch kunye nendawo ye-Incubator. Oku kunokuba yingenelo ikakhulu kwiilebhu ngendawo encinci okanye apho ukusebenza okukhulu khona kubalulekile.

I-5. Izixhobo zokuvumela

Ukuhambelana nezixhobo zelebhu esele zikho yenye into ebalulekileyo. Uninzi lweelebhu sele zinezixhobo ezilungiselelwe iipleyiti ezingama-96, ukusuka kwiirobhothi zemibhobho kwiiplate zokufunda. Ukutshintsha ukuya kwiipleyiti ezingama-384 kunokufuna izixhobo ezitsha okanye uhlengahlengiso kwiinkqubo ezikhoyo, ezinokuba neendleko kwaye zichitha ixesha. Ke ngoko, iilebhu kufuneka zihlolisise ngononophelo ukuba ngaba izibonelelo zokutshintshela kwi-384-pile ivele ikhuphe le miceli mngeni.

Ukuqukumbela

Ekugqibeleni, isigqibo phakathi kokusebenzisa i-96-okanye i-384-hinges eziqengqelekayo kwiimfuno ezithile zelebhu kunye nohlobo lovavanyo lwenziwa. Uvavanyo olufuna imiqulu emikhulu kwaye apho ubuntununtunu kunye nokuzala kugxeke, iipleyiti ezingama-96 zinokuba lolona khetho lungcono. Kwelinye icala, ukusetyenziswa kweendlela zokungena kwixabiso eliphezulu kunye nokusebenza kweendleko ngokwendlela yokusetyenziswa ngokutsha, iipleyiti ezingama-384-zinokuphucula kakhulu ukusebenza kwelebhu. Iilabhoratri kufuneka zizilinganise ezi zinto ngononophelo, ziqwalasele iimeko zabo ezizodwa, ukwenza ukhetho olulwaziyo noluphumelele.